Genomic organization and expression of two independent gene arrays coding for two antigenic acidic ribosomal proteins of Leishmania
In the present paper we describe the isolation and characterization of four novel genes of the parasitic protozoan Leishmania infantum. These genes are organized as two independent gene clusters, and they are related by nucleotide sequence to eukaryotic genes encoding acidic ribosomal proteins. Each...
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| Veröffentlicht in: | The Journal of biological chemistry 1993-10, Vol.268 (29), p.21835-21843 |
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| creator | SOTO, M REQUENA, J. M GARCIA, M GOMEZ, L. C NAVARRETE, I ALONSO, C |
| description | In the present paper we describe the isolation and characterization of four novel genes of the parasitic protozoan Leishmania
infantum. These genes are organized as two independent gene clusters, and they are related by nucleotide sequence to eukaryotic
genes encoding acidic ribosomal proteins. Each gene cluster contains two tandemly linked genes coding for identical proteins.
Each of the proteins coded by the gene clusters (called LiP and LiP') are highly divergent in sequence, showing the characteristic
features of eukaryotic P-proteins from the P2 group. In spite of the sequence conservation of the coding regions of each of
the genes in the cluster, the 5'- and 3'-untranslated regions are heterogeneous in sequence. The analysis of the expression
of these genes indicates that logarithmic phase promastigotes show increased levels of LiP- and LiP'-specific transcripts
compared with stationary phase promastigotes. The steady state RNA levels of the LiP and LiP' genes show a similar dependence
of the growth phase of the parasite. Using specific probes for the divergent 3'-untranslated regions of each of the genes,
it was found that the abundance of the mature transcripts is different even when the transcripts are derived from the same
gene cluster. These findings probably indicate that the 3'-untranslated regions may influence the stability or turnover of
the transcripts derived from both LiP and LiP' gene clusters. |
| doi_str_mv | 10.1016/s0021-9258(20)80617-6 |
| format | Article |
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infantum. These genes are organized as two independent gene clusters, and they are related by nucleotide sequence to eukaryotic
genes encoding acidic ribosomal proteins. Each gene cluster contains two tandemly linked genes coding for identical proteins.
Each of the proteins coded by the gene clusters (called LiP and LiP') are highly divergent in sequence, showing the characteristic
features of eukaryotic P-proteins from the P2 group. In spite of the sequence conservation of the coding regions of each of
the genes in the cluster, the 5'- and 3'-untranslated regions are heterogeneous in sequence. The analysis of the expression
of these genes indicates that logarithmic phase promastigotes show increased levels of LiP- and LiP'-specific transcripts
compared with stationary phase promastigotes. The steady state RNA levels of the LiP and LiP' genes show a similar dependence
of the growth phase of the parasite. Using specific probes for the divergent 3'-untranslated regions of each of the genes,
it was found that the abundance of the mature transcripts is different even when the transcripts are derived from the same
gene cluster. These findings probably indicate that the 3'-untranslated regions may influence the stability or turnover of
the transcripts derived from both LiP and LiP' gene clusters.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/s0021-9258(20)80617-6</identifier><identifier>PMID: 8408038</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Amino Acid Sequence ; Animals ; Antigens, Protozoan - immunology ; Base Sequence ; Biological and medical sciences ; Cell structures and functions ; DNA, Complementary ; Fundamental and applied biological sciences. Psychology ; Genes, Protozoan ; Leishmania infantum ; Leishmania infantum - genetics ; Molecular and cellular biology ; Molecular Sequence Data ; Multigene Family ; Protozoan Proteins - genetics ; Protozoan Proteins - immunology ; Ribosomal Proteins - genetics ; Ribosomal Proteins - immunology ; Ribosome ; RNA, Messenger - metabolism ; RNA, Protozoan - metabolism ; Sequence Homology, Amino Acid</subject><ispartof>The Journal of biological chemistry, 1993-10, Vol.268 (29), p.21835-21843</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4216-b4edd8b5dc5cb2e5fc1aada305df78782d1a43d7b171ccf65fc6f201b9b0fc3f3</citedby><cites>FETCH-LOGICAL-c4216-b4edd8b5dc5cb2e5fc1aada305df78782d1a43d7b171ccf65fc6f201b9b0fc3f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3814142$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8408038$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SOTO, M</creatorcontrib><creatorcontrib>REQUENA, J. M</creatorcontrib><creatorcontrib>GARCIA, M</creatorcontrib><creatorcontrib>GOMEZ, L. C</creatorcontrib><creatorcontrib>NAVARRETE, I</creatorcontrib><creatorcontrib>ALONSO, C</creatorcontrib><title>Genomic organization and expression of two independent gene arrays coding for two antigenic acidic ribosomal proteins of Leishmania</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>In the present paper we describe the isolation and characterization of four novel genes of the parasitic protozoan Leishmania
infantum. These genes are organized as two independent gene clusters, and they are related by nucleotide sequence to eukaryotic
genes encoding acidic ribosomal proteins. Each gene cluster contains two tandemly linked genes coding for identical proteins.
Each of the proteins coded by the gene clusters (called LiP and LiP') are highly divergent in sequence, showing the characteristic
features of eukaryotic P-proteins from the P2 group. In spite of the sequence conservation of the coding regions of each of
the genes in the cluster, the 5'- and 3'-untranslated regions are heterogeneous in sequence. The analysis of the expression
of these genes indicates that logarithmic phase promastigotes show increased levels of LiP- and LiP'-specific transcripts
compared with stationary phase promastigotes. The steady state RNA levels of the LiP and LiP' genes show a similar dependence
of the growth phase of the parasite. Using specific probes for the divergent 3'-untranslated regions of each of the genes,
it was found that the abundance of the mature transcripts is different even when the transcripts are derived from the same
gene cluster. These findings probably indicate that the 3'-untranslated regions may influence the stability or turnover of
the transcripts derived from both LiP and LiP' gene clusters.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antigens, Protozoan - immunology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell structures and functions</subject><subject>DNA, Complementary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Protozoan</subject><subject>Leishmania infantum</subject><subject>Leishmania infantum - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - immunology</subject><subject>Ribosomal Proteins - genetics</subject><subject>Ribosomal Proteins - immunology</subject><subject>Ribosome</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Protozoan - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9rFDEYxoModVv9CIUcROxhNH8mmcxRSq3CggcVvIVM8mY3MpOsySxtvfaLN9Mu67E55CU8v_d5Ag9C55R8pITKT4UQRpueCfWBkQtFJO0a-QKtKFG84YL-folWR-Q1Oi3lD6mn7ekJOlEtUYSrFbq_hpimYHHKGxPDPzOHFLGJDsPtLkMpyzN5PN8kHKKDHdQrzngDEbDJ2dwVbJMLcYN9yo-YiXOocvU0Nrg6chhSSZMZ8S6nGUIsi-MaQtlONdO8Qa-8GQu8Pcwz9OvL1c_Lr836-_W3y8_rxraMymZowTk1CGeFHRgIb6kxznAinO9Up5ijpuWuG2hHrfWyAtIzQod-IN5yz8_Q-yff-o2_eyiznkKxMI4mQtoX3UlChBTiWZBK1fO-bSsonkCbUykZvN7lMJl8pynRS0v6x1KBXirQjOjHlrSse-eHgP0wgTtuHWqp-ruDboo1o88m2lCOGFe0pS37j23DZnsTMughJLuFSTNZ83rNqOKCPwB98KnO</recordid><startdate>19931015</startdate><enddate>19931015</enddate><creator>SOTO, M</creator><creator>REQUENA, J. M</creator><creator>GARCIA, M</creator><creator>GOMEZ, L. C</creator><creator>NAVARRETE, I</creator><creator>ALONSO, C</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19931015</creationdate><title>Genomic organization and expression of two independent gene arrays coding for two antigenic acidic ribosomal proteins of Leishmania</title><author>SOTO, M ; REQUENA, J. M ; GARCIA, M ; GOMEZ, L. C ; NAVARRETE, I ; ALONSO, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4216-b4edd8b5dc5cb2e5fc1aada305df78782d1a43d7b171ccf65fc6f201b9b0fc3f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antigens, Protozoan - immunology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell structures and functions</topic><topic>DNA, Complementary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Protozoan</topic><topic>Leishmania infantum</topic><topic>Leishmania infantum - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Protozoan Proteins - genetics</topic><topic>Protozoan Proteins - immunology</topic><topic>Ribosomal Proteins - genetics</topic><topic>Ribosomal Proteins - immunology</topic><topic>Ribosome</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Protozoan - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SOTO, M</creatorcontrib><creatorcontrib>REQUENA, J. M</creatorcontrib><creatorcontrib>GARCIA, M</creatorcontrib><creatorcontrib>GOMEZ, L. C</creatorcontrib><creatorcontrib>NAVARRETE, I</creatorcontrib><creatorcontrib>ALONSO, C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SOTO, M</au><au>REQUENA, J. M</au><au>GARCIA, M</au><au>GOMEZ, L. C</au><au>NAVARRETE, I</au><au>ALONSO, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic organization and expression of two independent gene arrays coding for two antigenic acidic ribosomal proteins of Leishmania</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1993-10-15</date><risdate>1993</risdate><volume>268</volume><issue>29</issue><spage>21835</spage><epage>21843</epage><pages>21835-21843</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>In the present paper we describe the isolation and characterization of four novel genes of the parasitic protozoan Leishmania
infantum. These genes are organized as two independent gene clusters, and they are related by nucleotide sequence to eukaryotic
genes encoding acidic ribosomal proteins. Each gene cluster contains two tandemly linked genes coding for identical proteins.
Each of the proteins coded by the gene clusters (called LiP and LiP') are highly divergent in sequence, showing the characteristic
features of eukaryotic P-proteins from the P2 group. In spite of the sequence conservation of the coding regions of each of
the genes in the cluster, the 5'- and 3'-untranslated regions are heterogeneous in sequence. The analysis of the expression
of these genes indicates that logarithmic phase promastigotes show increased levels of LiP- and LiP'-specific transcripts
compared with stationary phase promastigotes. The steady state RNA levels of the LiP and LiP' genes show a similar dependence
of the growth phase of the parasite. Using specific probes for the divergent 3'-untranslated regions of each of the genes,
it was found that the abundance of the mature transcripts is different even when the transcripts are derived from the same
gene cluster. These findings probably indicate that the 3'-untranslated regions may influence the stability or turnover of
the transcripts derived from both LiP and LiP' gene clusters.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>8408038</pmid><doi>10.1016/s0021-9258(20)80617-6</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Antigens, Protozoan - immunology Base Sequence Biological and medical sciences Cell structures and functions DNA, Complementary Fundamental and applied biological sciences. Psychology Genes, Protozoan Leishmania infantum Leishmania infantum - genetics Molecular and cellular biology Molecular Sequence Data Multigene Family Protozoan Proteins - genetics Protozoan Proteins - immunology Ribosomal Proteins - genetics Ribosomal Proteins - immunology Ribosome RNA, Messenger - metabolism RNA, Protozoan - metabolism Sequence Homology, Amino Acid |
| title | Genomic organization and expression of two independent gene arrays coding for two antigenic acidic ribosomal proteins of Leishmania |
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