The structure of a bovine lung cGMP-binding, cGMP-specific phosphodiesterase deduced from a cDNA clone

Polymerase chain reaction (PCR) methodology and cDNA library screening were used to isolate a cDNA clone encoding a cGMP-binding, cGMP-specific phosphodiesterase (cGB-PDE) from bovine lung. Degenerate oligonucleotides based on cGB-PDE peptide sequences were used as primers for a PCR reaction with bo...

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Veröffentlicht in:	The Journal of biological chemistry 1993-10, Vol.268 (30), p.22863-22873
Hauptverfasser:	McAllister-Lucas, L.M., Sonnenburg, W.K., Kadlecek, A, Seger, D, Trong, H.L., Colbran, J.L., Thomas, M.K., Walsh, K.A., Francis, S.H., Corbin, J.D.
Format:	Artikel
Sprache:	eng
Schlagworte:	3',5'-Cyclic-GMP Phosphodiesterases - chemistry 3',5'-Cyclic-GMP Phosphodiesterases - genetics 3',5'-Cyclic-GMP Phosphodiesterases - metabolism ADN Amino Acid Sequence Analytical, structural and metabolic biochemistry Animals Base Sequence Binding Sites Biological and medical sciences Blotting, Northern BOVIN Cattle Cell Line Cloning, Molecular Conserved Sequence Cyclic GMP - metabolism DNA, Complementary - metabolism Enzymes and enzyme inhibitors ESTERASAS ESTERASE Fundamental and applied biological sciences. Psychology GANADO BOVINO Gene Library Hydrolases Kinetics Lung - enzymology Molecular Sequence Data Polymerase Chain Reaction - methods POUMON PULMONES Recombinant Proteins - metabolism RNA, Messenger - biosynthesis RNA, Messenger - isolation & purification RNA, Messenger - metabolism SECUENCIA NUCLEICA Sequence Homology, Amino Acid SEQUENCE NUCLEIQUE Substrate Specificity Transfection
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