cis-diamminedichloroplatinum-mediated crosslinking of nuclear proteins to DNA is cell cycle specific
Immunochemical analysis was employed to investigate the cell cycle-dependent protein-DNA crosslinking by cis-diamminedichloroplatinum II ( cis-DDP), in HeLa-S 3 cells. Cells synchronized by double thymidine block or hydroxyurea were released into S phase and incubated at 2-h intervals with cis-DDP a...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1985-02, Vol.237 (1), p.202-207 |
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| container_title | Archives of biochemistry and biophysics |
| container_volume | 237 |
| creator | Banjar, Zainy M. Hnilica, Lubomir S. Briggs, Robert C. Dominguez, Evelio Stein, Janet L. Stein, Gary S. |
| description | Immunochemical analysis was employed to investigate the cell cycle-dependent protein-DNA crosslinking by
cis-diamminedichloroplatinum II (
cis-DDP), in HeLa-S
3 cells. Cells synchronized by double thymidine block or hydroxyurea were released into S phase and incubated at 2-h intervals with
cis-DDP as they progressed through S
1, G
2, M, and then into G
1 and S phases of the subsequent cycle. Immunoblots of the DNA-crosslinked antigens reacted with antisera to 0.35
m NaCl extract or residue of HeLa S-phase nuclei revealed that several antigens changed their DNA-crosslinking pattern during the progression of HeLa cells through their reproductive cycle. |
| doi_str_mv | 10.1016/0003-9861(85)90270-X |
| format | Article |
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cis-diamminedichloroplatinum II (
cis-DDP), in HeLa-S
3 cells. Cells synchronized by double thymidine block or hydroxyurea were released into S phase and incubated at 2-h intervals with
cis-DDP as they progressed through S
1, G
2, M, and then into G
1 and S phases of the subsequent cycle. Immunoblots of the DNA-crosslinked antigens reacted with antisera to 0.35
m NaCl extract or residue of HeLa S-phase nuclei revealed that several antigens changed their DNA-crosslinking pattern during the progression of HeLa cells through their reproductive cycle.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/0003-9861(85)90270-X</identifier><identifier>PMID: 4038596</identifier><identifier>CODEN: ABBIA4</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Biological and medical sciences ; Cell Cycle ; Cell cycle, cell proliferation ; Cell physiology ; Cisplatin - pharmacology ; Cross-Linking Reagents ; DNA - metabolism ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; HeLa Cells - metabolism ; Humans ; Immunochemistry ; Molecular and cellular biology ; Nucleoproteins - metabolism ; Protein Binding - drug effects ; Thymidine - metabolism</subject><ispartof>Archives of biochemistry and biophysics, 1985-02, Vol.237 (1), p.202-207</ispartof><rights>1985</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-168f0ef4810525f42549dc739898c400e2c9a8961abfc80bb8eeec08040a4ffb3</citedby><cites>FETCH-LOGICAL-c386t-168f0ef4810525f42549dc739898c400e2c9a8961abfc80bb8eeec08040a4ffb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-9861(85)90270-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9105845$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4038596$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Banjar, Zainy M.</creatorcontrib><creatorcontrib>Hnilica, Lubomir S.</creatorcontrib><creatorcontrib>Briggs, Robert C.</creatorcontrib><creatorcontrib>Dominguez, Evelio</creatorcontrib><creatorcontrib>Stein, Janet L.</creatorcontrib><creatorcontrib>Stein, Gary S.</creatorcontrib><title>cis-diamminedichloroplatinum-mediated crosslinking of nuclear proteins to DNA is cell cycle specific</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>Immunochemical analysis was employed to investigate the cell cycle-dependent protein-DNA crosslinking by
cis-diamminedichloroplatinum II (
cis-DDP), in HeLa-S
3 cells. Cells synchronized by double thymidine block or hydroxyurea were released into S phase and incubated at 2-h intervals with
cis-DDP as they progressed through S
1, G
2, M, and then into G
1 and S phases of the subsequent cycle. Immunoblots of the DNA-crosslinked antigens reacted with antisera to 0.35
m NaCl extract or residue of HeLa S-phase nuclei revealed that several antigens changed their DNA-crosslinking pattern during the progression of HeLa cells through their reproductive cycle.</description><subject>Biological and medical sciences</subject><subject>Cell Cycle</subject><subject>Cell cycle, cell proliferation</subject><subject>Cell physiology</subject><subject>Cisplatin - pharmacology</subject><subject>Cross-Linking Reagents</subject><subject>DNA - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HeLa Cells - metabolism</subject><subject>Humans</subject><subject>Immunochemistry</subject><subject>Molecular and cellular biology</subject><subject>Nucleoproteins - metabolism</subject><subject>Protein Binding - drug effects</subject><subject>Thymidine - metabolism</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVJSTdp_kELOoTSHtyObFkrXQohTT8gJJcGchPyeJSoteWtZBfy76vNLnvsSaD3meGdh7E3Aj4KEOoTADSV0Uq81-0HA_UaqvsXbCXAqAoaLY_Y6oC8Yic5_wIQQqr6mB3LArRGrViPIVd9cOMYIvUBH4cpTZvBzSEuYzWWLzdTzzFNOQ8h_g7xgU-exwUHcolv0jRTiJnPE_9yc8FD5kjDwPGp5DxvCIMP-Jq99G7IdLZ_T9nd16ufl9-r69tvPy4vritstJorobQH8lILaOvWy7qVpsd1Y7TRKAGoRuO0UcJ1HjV0nSYiBA0SnPS-a07Zu93eUuvPQnm2Y8jbPi7StGS7bo2p1VoVUO7A57sSebtJYXTpyQqwW7l2a85uzVnd2me59r6Mvd3vX7qi5jC0t1ny833uMrrBJxeL3gNmyl1atgX7vMOouPgbKNmMgSIW2Ylwtv0U_t_jH-21lxk</recordid><startdate>19850215</startdate><enddate>19850215</enddate><creator>Banjar, Zainy M.</creator><creator>Hnilica, Lubomir S.</creator><creator>Briggs, Robert C.</creator><creator>Dominguez, Evelio</creator><creator>Stein, Janet L.</creator><creator>Stein, Gary S.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19850215</creationdate><title>cis-diamminedichloroplatinum-mediated crosslinking of nuclear proteins to DNA is cell cycle specific</title><author>Banjar, Zainy M. ; Hnilica, Lubomir S. ; Briggs, Robert C. ; Dominguez, Evelio ; Stein, Janet L. ; Stein, Gary S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-168f0ef4810525f42549dc739898c400e2c9a8961abfc80bb8eeec08040a4ffb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Biological and medical sciences</topic><topic>Cell Cycle</topic><topic>Cell cycle, cell proliferation</topic><topic>Cell physiology</topic><topic>Cisplatin - pharmacology</topic><topic>Cross-Linking Reagents</topic><topic>DNA - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HeLa Cells - metabolism</topic><topic>Humans</topic><topic>Immunochemistry</topic><topic>Molecular and cellular biology</topic><topic>Nucleoproteins - metabolism</topic><topic>Protein Binding - drug effects</topic><topic>Thymidine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Banjar, Zainy M.</creatorcontrib><creatorcontrib>Hnilica, Lubomir S.</creatorcontrib><creatorcontrib>Briggs, Robert C.</creatorcontrib><creatorcontrib>Dominguez, Evelio</creatorcontrib><creatorcontrib>Stein, Janet L.</creatorcontrib><creatorcontrib>Stein, Gary S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Banjar, Zainy M.</au><au>Hnilica, Lubomir S.</au><au>Briggs, Robert C.</au><au>Dominguez, Evelio</au><au>Stein, Janet L.</au><au>Stein, Gary S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>cis-diamminedichloroplatinum-mediated crosslinking of nuclear proteins to DNA is cell cycle specific</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1985-02-15</date><risdate>1985</risdate><volume>237</volume><issue>1</issue><spage>202</spage><epage>207</epage><pages>202-207</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><coden>ABBIA4</coden><abstract>Immunochemical analysis was employed to investigate the cell cycle-dependent protein-DNA crosslinking by
cis-diamminedichloroplatinum II (
cis-DDP), in HeLa-S
3 cells. Cells synchronized by double thymidine block or hydroxyurea were released into S phase and incubated at 2-h intervals with
cis-DDP as they progressed through S
1, G
2, M, and then into G
1 and S phases of the subsequent cycle. Immunoblots of the DNA-crosslinked antigens reacted with antisera to 0.35
m NaCl extract or residue of HeLa S-phase nuclei revealed that several antigens changed their DNA-crosslinking pattern during the progression of HeLa cells through their reproductive cycle.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>4038596</pmid><doi>10.1016/0003-9861(85)90270-X</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-9861 |
| ispartof | Archives of biochemistry and biophysics, 1985-02, Vol.237 (1), p.202-207 |
| issn | 0003-9861 1096-0384 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Biological and medical sciences Cell Cycle Cell cycle, cell proliferation Cell physiology Cisplatin - pharmacology Cross-Linking Reagents DNA - metabolism Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology HeLa Cells - metabolism Humans Immunochemistry Molecular and cellular biology Nucleoproteins - metabolism Protein Binding - drug effects Thymidine - metabolism |
| title | cis-diamminedichloroplatinum-mediated crosslinking of nuclear proteins to DNA is cell cycle specific |
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