Direct evidence for the partial dehydration of phosphatidylethanolamine bilayers on approaching the hexagonal phase

X-ray diffraction studies on oriented multilayers of 1-palmitoyl-2-oleoylphosphatidylethanolamine (POPE) in the lamellar gel (L beta) and inverted hexagonal (HII) phases at various temperatures (5-50 degrees C) and relative humidities (0-100%) are reported. One-dimensional electron density profiles...

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Veröffentlicht in:Biochemistry (Easton) 1993-10, Vol.32 (40), p.10700-10707
Hauptverfasser: Katsaras, John, Jeffrey, Kenneth R, Yang, Daniel S. C, Epand, Richard M
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creator Katsaras, John
Jeffrey, Kenneth R
Yang, Daniel S. C
Epand, Richard M
description X-ray diffraction studies on oriented multilayers of 1-palmitoyl-2-oleoylphosphatidylethanolamine (POPE) in the lamellar gel (L beta) and inverted hexagonal (HII) phases at various temperatures (5-50 degrees C) and relative humidities (0-100%) are reported. One-dimensional electron density profiles of the L beta phase bilayers were constructed to a resolution of better than 4 A using direct methods to solve for the phase problem. In addition, the electron density profiles were fitted favorably using a model in which the atomic groups were assumed to be Gaussian distributed [Wiener, M. C., & White, S. H. (1992) Biophys. J. 61, 434-447]. The X-ray data clearly demonstrate that, at 100% relative humidity (RH), POPE samples exist in two distinct L beta phases, differing primarily in the amount of water between the lamellae. As the hexagonal phase transition temperature is approached, 100% RH POPE samples partially dehydrate, releasing approximately 5 water molecules per phospholipid and experiencing on average a 3-A decrease in repeat spacing. The lower temperature hydrated L beta phase POPE electron density distribution resembles that obtained from the L beta phase 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) bilayers and is unlike the partially dehydrated POPE bilayers.
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C</creatorcontrib><creatorcontrib>Epand, Richard M</creatorcontrib><title>Direct evidence for the partial dehydration of phosphatidylethanolamine bilayers on approaching the hexagonal phase</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>X-ray diffraction studies on oriented multilayers of 1-palmitoyl-2-oleoylphosphatidylethanolamine (POPE) in the lamellar gel (L beta) and inverted hexagonal (HII) phases at various temperatures (5-50 degrees C) and relative humidities (0-100%) are reported. One-dimensional electron density profiles of the L beta phase bilayers were constructed to a resolution of better than 4 A using direct methods to solve for the phase problem. In addition, the electron density profiles were fitted favorably using a model in which the atomic groups were assumed to be Gaussian distributed [Wiener, M. C., &amp; White, S. H. (1992) Biophys. J. 61, 434-447]. 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Psychology</subject><subject>Humidity</subject><subject>Lipid Bilayers</subject><subject>Mathematics</subject><subject>Membrane physicochemistry</subject><subject>Models, Theoretical</subject><subject>Molecular biophysics</subject><subject>Molecular Conformation</subject><subject>Phosphatidylcholines - chemistry</subject><subject>Phosphatidylethanolamines - chemistry</subject><subject>X-Ray Diffraction</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkM1v1DAQxS0EKkvhxBkpBwQHFBgn_lgfUYEWUUElyoWLNbEnjUs2DnYWdf97DLtaceA0M36_eRo_xp5yeM2h4W-6AACGY-nvsRWXDdTCGHmfrcq7qhuj4CF7lPNtGQVoccJO1q0xDZcrlt-FRG6p6FfwNDmq-piqZaBqxrQEHCtPw84nXEKcqthX8xDzPJTR70ZaBpziiJswUdWFEXeUclU4nOcU0Q1huvnrNdAd3sSpuJXVTI_Zgx7HTE8O9ZR9-_D--uyivvxy_vHs7WWNQpil9p3qOafWgZEKGnSNIofCKKe4BqlLIyT4TnTg-doLgQjaC-07roxuoD1lL_a-5ZqfW8qL3YTsaBxxorjNVkuzXoMQBXy1B12KOSfq7ZzCBtPOcrB_Irb_RFzoZwfbbbchf2QPmRb9-UHH7HDsE04u5CPW6vIdqQpW77GQF7o7yph-WKVbLe311Vd71QD__Oni3H4v_Ms9jy7b27hNJc_83wN_Aw3AoFw</recordid><startdate>19931012</startdate><enddate>19931012</enddate><creator>Katsaras, John</creator><creator>Jeffrey, Kenneth R</creator><creator>Yang, Daniel S. 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The lower temperature hydrated L beta phase POPE electron density distribution resembles that obtained from the L beta phase 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) bilayers and is unlike the partially dehydrated POPE bilayers.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>8399215</pmid><doi>10.1021/bi00091a021</doi><tpages>8</tpages></addata></record>
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source MEDLINE; American Chemical Society Journals
subjects Artificial membranes and reconstituted systems
Biological and medical sciences
Desiccation
Fundamental and applied biological sciences. Psychology
Humidity
Lipid Bilayers
Mathematics
Membrane physicochemistry
Models, Theoretical
Molecular biophysics
Molecular Conformation
Phosphatidylcholines - chemistry
Phosphatidylethanolamines - chemistry
X-Ray Diffraction
title Direct evidence for the partial dehydration of phosphatidylethanolamine bilayers on approaching the hexagonal phase
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