Microfluidic direct injection method for analysis of urinary 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) using molecularly imprinted polymers coupled on-line with LC–MS/MS
The work described in this paper involves development of a high-throughput on-line microfluidic sample extraction method using capillary micro-columns packed with MIP beads coupled with tandem mass spectrometry for the analysis of urinary NNAL. The method was optimized and matrix effects were evalua...
Gespeichert in:
| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2011-01, Vol.54 (2), p.368-378 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 378 |
|---|---|
| container_issue | 2 |
| container_start_page | 368 |
| container_title | Journal of pharmaceutical and biomedical analysis |
| container_volume | 54 |
| creator | Shah, Kumar A. Peoples, Michael C. Halquist, Matthew S. Rutan, Sarah C. Karnes, H. Thomas |
| description | The work described in this paper involves development of a high-throughput on-line microfluidic sample extraction method using capillary micro-columns packed with MIP beads coupled with tandem mass spectrometry for the analysis of urinary NNAL. The method was optimized and matrix effects were evaluated and resolved. The method enabled low sample volume (200
μL) and rapid analysis of urinary NNAL by direct injection onto the microfluidic column packed with molecularly imprinted beads engineered to NNAL. The method was validated according to the FDA bioanalytical method validation guidance. The dynamic range extended from 20.0 to 2500.0
pg/mL with a percent relative error of ±5.9% and a run time of 7.00
min. The lower limit of quantitation was 20.0
pg/mL. The method was used for the analysis of NNAL and NNAL–Gluc concentrations in smokers’ urine. |
| doi_str_mv | 10.1016/j.jpba.2010.09.001 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_759877825</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0731708510005133</els_id><sourcerecordid>759877825</sourcerecordid><originalsourceid>FETCH-LOGICAL-c409t-fa2d1a69c94e624be9327d19ef2de28a90788ce8ceccc8ae667f4909a7b5916c3</originalsourceid><addsrcrecordid>eNp9kUuO1DAQhiMEYpqBC7AAbxA9i_TYztMSm1GLl9Q9LJqR2FmOU55xy7EzdgLKjjtwFw7ESXCUBnZIlkouffXX40-S5wRvCCbl5XFz7BuxoTgmMNtgTB4kK1JXWUrL_MvDZIWrjKQVrouz5EkIR4xxQVj-ODmjmNGSknKV_Nxr6Z0yo261RK32IAek7TEG7SzqYLhzLVLOI2GFmYIOyCk0em2Fn1CermdiMlYP3gXRaesuUpKus7SfvG4nM_-acRDWGbS-vr7aXaAxaHuLOmdAjkZ4MyHd9VFwgBb1zkwd-ICkG3sTE86mRltA3_Rwh3bbX99_7A-X-8PT5JESJsCzUzxPbt69_bz9kO4-vf-4vdqlMsdsSJWgLRElkyyHkuYNsIxWLWGgaAu0FgxXdS0hPillLaAsK5UzzETVFIyUMjtPXi-6vXf3I4SBdzpIMEZYcGPgVcHqqqppEUm6kPGcIXhQPO7UxSNxgvlsFz_y2S4-28Ux49GuWPTiJD82HbR_S_74E4FXJ0AEKYzywkod_nFZjuPMM_dy4ZRwXNz6yNwcYqcidqE4K-pIvFkIiOf6qsHzIDVYCYvnvHX6f5P-BkH0wCk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>759877825</pqid></control><display><type>article</type><title>Microfluidic direct injection method for analysis of urinary 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) using molecularly imprinted polymers coupled on-line with LC–MS/MS</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Shah, Kumar A. ; Peoples, Michael C. ; Halquist, Matthew S. ; Rutan, Sarah C. ; Karnes, H. Thomas</creator><creatorcontrib>Shah, Kumar A. ; Peoples, Michael C. ; Halquist, Matthew S. ; Rutan, Sarah C. ; Karnes, H. Thomas</creatorcontrib><description>The work described in this paper involves development of a high-throughput on-line microfluidic sample extraction method using capillary micro-columns packed with MIP beads coupled with tandem mass spectrometry for the analysis of urinary NNAL. The method was optimized and matrix effects were evaluated and resolved. The method enabled low sample volume (200
μL) and rapid analysis of urinary NNAL by direct injection onto the microfluidic column packed with molecularly imprinted beads engineered to NNAL. The method was validated according to the FDA bioanalytical method validation guidance. The dynamic range extended from 20.0 to 2500.0
pg/mL with a percent relative error of ±5.9% and a run time of 7.00
min. The lower limit of quantitation was 20.0
pg/mL. The method was used for the analysis of NNAL and NNAL–Gluc concentrations in smokers’ urine.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2010.09.001</identifier><identifier>PMID: 20926216</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adult ; Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Butanols - urine ; Calibration ; Chromatography, Liquid - methods ; Female ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Glucuronates - urine ; Humans ; LC–MS/MS ; Male ; Medical sciences ; Microfluidics - methods ; Middle Aged ; Molecular Imprinting ; Molecular Structure ; Molecular Weight ; Molecularly imprinted polymer ; Nitrosamines - urine ; NNAL ; On-line microfluidic solid phase extraction ; Online Systems ; Pharmacology. Drug treatments ; polymers ; Polymers - chemistry ; Pyridines - urine ; Quality Control ; rapid methods ; Reference Standards ; Reproducibility of Results ; tandem mass spectrometry ; Tandem Mass Spectrometry - methods ; Time Factors ; Tobacco specific nitrosamine ; urine</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2011-01, Vol.54 (2), p.368-378</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-fa2d1a69c94e624be9327d19ef2de28a90788ce8ceccc8ae667f4909a7b5916c3</citedby><cites>FETCH-LOGICAL-c409t-fa2d1a69c94e624be9327d19ef2de28a90788ce8ceccc8ae667f4909a7b5916c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jpba.2010.09.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23406676$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20926216$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shah, Kumar A.</creatorcontrib><creatorcontrib>Peoples, Michael C.</creatorcontrib><creatorcontrib>Halquist, Matthew S.</creatorcontrib><creatorcontrib>Rutan, Sarah C.</creatorcontrib><creatorcontrib>Karnes, H. Thomas</creatorcontrib><title>Microfluidic direct injection method for analysis of urinary 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) using molecularly imprinted polymers coupled on-line with LC–MS/MS</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>The work described in this paper involves development of a high-throughput on-line microfluidic sample extraction method using capillary micro-columns packed with MIP beads coupled with tandem mass spectrometry for the analysis of urinary NNAL. The method was optimized and matrix effects were evaluated and resolved. The method enabled low sample volume (200
μL) and rapid analysis of urinary NNAL by direct injection onto the microfluidic column packed with molecularly imprinted beads engineered to NNAL. The method was validated according to the FDA bioanalytical method validation guidance. The dynamic range extended from 20.0 to 2500.0
pg/mL with a percent relative error of ±5.9% and a run time of 7.00
min. The lower limit of quantitation was 20.0
pg/mL. The method was used for the analysis of NNAL and NNAL–Gluc concentrations in smokers’ urine.</description><subject>Adult</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Butanols - urine</subject><subject>Calibration</subject><subject>Chromatography, Liquid - methods</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Glucuronates - urine</subject><subject>Humans</subject><subject>LC–MS/MS</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microfluidics - methods</subject><subject>Middle Aged</subject><subject>Molecular Imprinting</subject><subject>Molecular Structure</subject><subject>Molecular Weight</subject><subject>Molecularly imprinted polymer</subject><subject>Nitrosamines - urine</subject><subject>NNAL</subject><subject>On-line microfluidic solid phase extraction</subject><subject>Online Systems</subject><subject>Pharmacology. Drug treatments</subject><subject>polymers</subject><subject>Polymers - chemistry</subject><subject>Pyridines - urine</subject><subject>Quality Control</subject><subject>rapid methods</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>tandem mass spectrometry</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Time Factors</subject><subject>Tobacco specific nitrosamine</subject><subject>urine</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUuO1DAQhiMEYpqBC7AAbxA9i_TYztMSm1GLl9Q9LJqR2FmOU55xy7EzdgLKjjtwFw7ESXCUBnZIlkouffXX40-S5wRvCCbl5XFz7BuxoTgmMNtgTB4kK1JXWUrL_MvDZIWrjKQVrouz5EkIR4xxQVj-ODmjmNGSknKV_Nxr6Z0yo261RK32IAek7TEG7SzqYLhzLVLOI2GFmYIOyCk0em2Fn1CermdiMlYP3gXRaesuUpKus7SfvG4nM_-acRDWGbS-vr7aXaAxaHuLOmdAjkZ4MyHd9VFwgBb1zkwd-ICkG3sTE86mRltA3_Rwh3bbX99_7A-X-8PT5JESJsCzUzxPbt69_bz9kO4-vf-4vdqlMsdsSJWgLRElkyyHkuYNsIxWLWGgaAu0FgxXdS0hPillLaAsK5UzzETVFIyUMjtPXi-6vXf3I4SBdzpIMEZYcGPgVcHqqqppEUm6kPGcIXhQPO7UxSNxgvlsFz_y2S4-28Ux49GuWPTiJD82HbR_S_74E4FXJ0AEKYzywkod_nFZjuPMM_dy4ZRwXNz6yNwcYqcidqE4K-pIvFkIiOf6qsHzIDVYCYvnvHX6f5P-BkH0wCk</recordid><startdate>20110125</startdate><enddate>20110125</enddate><creator>Shah, Kumar A.</creator><creator>Peoples, Michael C.</creator><creator>Halquist, Matthew S.</creator><creator>Rutan, Sarah C.</creator><creator>Karnes, H. Thomas</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110125</creationdate><title>Microfluidic direct injection method for analysis of urinary 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) using molecularly imprinted polymers coupled on-line with LC–MS/MS</title><author>Shah, Kumar A. ; Peoples, Michael C. ; Halquist, Matthew S. ; Rutan, Sarah C. ; Karnes, H. Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-fa2d1a69c94e624be9327d19ef2de28a90788ce8ceccc8ae667f4909a7b5916c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adult</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Butanols - urine</topic><topic>Calibration</topic><topic>Chromatography, Liquid - methods</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Glucuronates - urine</topic><topic>Humans</topic><topic>LC–MS/MS</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microfluidics - methods</topic><topic>Middle Aged</topic><topic>Molecular Imprinting</topic><topic>Molecular Structure</topic><topic>Molecular Weight</topic><topic>Molecularly imprinted polymer</topic><topic>Nitrosamines - urine</topic><topic>NNAL</topic><topic>On-line microfluidic solid phase extraction</topic><topic>Online Systems</topic><topic>Pharmacology. Drug treatments</topic><topic>polymers</topic><topic>Polymers - chemistry</topic><topic>Pyridines - urine</topic><topic>Quality Control</topic><topic>rapid methods</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>tandem mass spectrometry</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Time Factors</topic><topic>Tobacco specific nitrosamine</topic><topic>urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shah, Kumar A.</creatorcontrib><creatorcontrib>Peoples, Michael C.</creatorcontrib><creatorcontrib>Halquist, Matthew S.</creatorcontrib><creatorcontrib>Rutan, Sarah C.</creatorcontrib><creatorcontrib>Karnes, H. Thomas</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shah, Kumar A.</au><au>Peoples, Michael C.</au><au>Halquist, Matthew S.</au><au>Rutan, Sarah C.</au><au>Karnes, H. Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microfluidic direct injection method for analysis of urinary 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) using molecularly imprinted polymers coupled on-line with LC–MS/MS</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2011-01-25</date><risdate>2011</risdate><volume>54</volume><issue>2</issue><spage>368</spage><epage>378</epage><pages>368-378</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>The work described in this paper involves development of a high-throughput on-line microfluidic sample extraction method using capillary micro-columns packed with MIP beads coupled with tandem mass spectrometry for the analysis of urinary NNAL. The method was optimized and matrix effects were evaluated and resolved. The method enabled low sample volume (200
μL) and rapid analysis of urinary NNAL by direct injection onto the microfluidic column packed with molecularly imprinted beads engineered to NNAL. The method was validated according to the FDA bioanalytical method validation guidance. The dynamic range extended from 20.0 to 2500.0
pg/mL with a percent relative error of ±5.9% and a run time of 7.00
min. The lower limit of quantitation was 20.0
pg/mL. The method was used for the analysis of NNAL and NNAL–Gluc concentrations in smokers’ urine.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>20926216</pmid><doi>10.1016/j.jpba.2010.09.001</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0731-7085 |
| ispartof | Journal of pharmaceutical and biomedical analysis, 2011-01, Vol.54 (2), p.368-378 |
| issn | 0731-7085 1873-264X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_759877825 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adult Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Butanols - urine Calibration Chromatography, Liquid - methods Female Fundamental and applied biological sciences. Psychology General pharmacology Glucuronates - urine Humans LC–MS/MS Male Medical sciences Microfluidics - methods Middle Aged Molecular Imprinting Molecular Structure Molecular Weight Molecularly imprinted polymer Nitrosamines - urine NNAL On-line microfluidic solid phase extraction Online Systems Pharmacology. Drug treatments polymers Polymers - chemistry Pyridines - urine Quality Control rapid methods Reference Standards Reproducibility of Results tandem mass spectrometry Tandem Mass Spectrometry - methods Time Factors Tobacco specific nitrosamine urine |
| title | Microfluidic direct injection method for analysis of urinary 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) using molecularly imprinted polymers coupled on-line with LC–MS/MS |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T12%3A07%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Microfluidic%20direct%20injection%20method%20for%20analysis%20of%20urinary%204-(methylnitrosamino)-1-(3-pyridyl)-1-butanol%20(NNAL)%20using%20molecularly%20imprinted%20polymers%20coupled%20on-line%20with%20LC%E2%80%93MS/MS&rft.jtitle=Journal%20of%20pharmaceutical%20and%20biomedical%20analysis&rft.au=Shah,%20Kumar%20A.&rft.date=2011-01-25&rft.volume=54&rft.issue=2&rft.spage=368&rft.epage=378&rft.pages=368-378&rft.issn=0731-7085&rft.eissn=1873-264X&rft.coden=JPBADA&rft_id=info:doi/10.1016/j.jpba.2010.09.001&rft_dat=%3Cproquest_cross%3E759877825%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=759877825&rft_id=info:pmid/20926216&rft_els_id=S0731708510005133&rfr_iscdi=true |