Enhanced Liver Regeneration Following Acute Hepatectomy by Low-Level Laser Therapy
Objective: The aim of the present study was to investigate the effect of low-level laser therapy (LLLT) on liver regeneration following hepatectomy. Background: LLLT has been found to modulate various biological processes. Materials and Methods: Twelve mature male rats were used. The liver was expos...
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| Veröffentlicht in: | Photomedicine and laser surgery 2010-10, Vol.28 (5), p.675-678 |
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| creator | Oron, U. Maltz, L. Tuby, H. Sorin, V. Czerniak, A. |
| description | Objective:
The aim of the present study was to investigate the effect of low-level laser therapy (LLLT) on liver regeneration following hepatectomy.
Background:
LLLT has been found to modulate various biological processes.
Materials and
Methods:
Twelve mature male rats were used. The liver was exposed, and 70% of it was excised. The rats were assigned randomly to two groups: control, non-laser treated, and experimental, laser-treated (diode [Ga-Al-As] laser 804 nm) group. For determination of newly formed blood vessels and proliferating cells, 5-Bromo-2′deoxyuridine (BrdU) was injected intraperitoneally. The rats were sacrificed 2 d post hepatectomy, and histological sections from each liver were processed for analysis of new blood-vessel formation using BrdU immunostaining kit. Mesenchymal stem cells (MSCs) were assessed using c-kit immunostaining. BrdU-labeled cells were counted as for estimation of newly formed hepatic cells.
Results:
It was found that the number of proliferating cells (BrdU positive cells) per area in the regenerating regions of the livers were significantly (
p
|
| doi_str_mv | 10.1089/pho.2009.2756 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_759876803</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A245661613</galeid><sourcerecordid>A245661613</sourcerecordid><originalsourceid>FETCH-LOGICAL-c403t-b4b27ecd106d5b20f8f70561888b6b251533da523ac31e2febd6f7f1e08f3f713</originalsourceid><addsrcrecordid>eNqFkctr3DAQh0VpadIkx16LoYeevNXDevi4hDwKhkJIz0KSR7sqtuRK3oT972uzSaFQKHOYYfh-w8CH0EeCNwSr9uu0TxuKcbuhkos36JxwLmvFOX67zk1b84aoM_ShlJ8Y01a27D06o7hllCh6jh5u4t5EB33VhSfI1QPsIEI2c0ixuk3DkJ5D3FVbd5ihuofJzODmNB4re6y69Fx38ARD1ZmyZB_3S3A6XqJ33gwFrl76Bfpxe_N4fV933---XW-72jWYzbVtLJXgeoJFzy3FXnmJuSBKKSss5YQz1htOmXGMAPVge-GlJ4CVZ14SdoG-nO5OOf06QJn1GIqDYTAR0qFoyVslhcJsIT-fyJ0ZQIfo05yNW2m9pQ0XggiyUpt_UEv1MAaXIviw7P8K1KeAy6mUDF5POYwmHzXBepWjFzl6laNXOQv_6eXhgx2h_0O_2lgAdgLWtYlxCGAhz_85-xtZLJoe</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>759876803</pqid></control><display><type>article</type><title>Enhanced Liver Regeneration Following Acute Hepatectomy by Low-Level Laser Therapy</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Oron, U. ; Maltz, L. ; Tuby, H. ; Sorin, V. ; Czerniak, A.</creator><creatorcontrib>Oron, U. ; Maltz, L. ; Tuby, H. ; Sorin, V. ; Czerniak, A.</creatorcontrib><description>Objective:
The aim of the present study was to investigate the effect of low-level laser therapy (LLLT) on liver regeneration following hepatectomy.
Background:
LLLT has been found to modulate various biological processes.
Materials and
Methods:
Twelve mature male rats were used. The liver was exposed, and 70% of it was excised. The rats were assigned randomly to two groups: control, non-laser treated, and experimental, laser-treated (diode [Ga-Al-As] laser 804 nm) group. For determination of newly formed blood vessels and proliferating cells, 5-Bromo-2′deoxyuridine (BrdU) was injected intraperitoneally. The rats were sacrificed 2 d post hepatectomy, and histological sections from each liver were processed for analysis of new blood-vessel formation using BrdU immunostaining kit. Mesenchymal stem cells (MSCs) were assessed using c-kit immunostaining. BrdU-labeled cells were counted as for estimation of newly formed hepatic cells.
Results:
It was found that the number of proliferating cells (BrdU positive cells) per area in the regenerating regions of the livers were significantly (
p
< 0.01) 2.6-fold higher in the laser-treated rats than in the control non-laser-treated rats. The density of the newly formed blood vessels and c-kit immunopositive cells in the regenerating area of the laser-treated livers was significantly (
p
< 0.01) 3.3- and 2.3-fold respectively higher than the control non-laser treated livers.
Conclusion:
It is concluded that LLLT following acute hepatectomy most probably stimulates a significant enhancement of liver regeneration conducive to both the formation of new hepatocytes and MSCs and angiogenesis in the regenerating liver.</description><identifier>ISSN: 1549-5418</identifier><identifier>EISSN: 1557-8550</identifier><identifier>DOI: 10.1089/pho.2009.2756</identifier><identifier>PMID: 20932182</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Acute Disease ; Analysis of Variance ; Animals ; Disease Models, Animal ; Hepatectomy ; Immunohistochemistry ; Lasers in surgery ; Lasers, Semiconductor - therapeutic use ; Liver ; Liver Regeneration - physiology ; Liver Regeneration - radiation effects ; Low-Level Light Therapy - methods ; Male ; Methods ; Original Articles ; Photomicrography ; Postoperative care ; Random Allocation ; Rats ; Rats, Inbred Strains ; Reference Values ; Regeneration ; Technology application</subject><ispartof>Photomedicine and laser surgery, 2010-10, Vol.28 (5), p.675-678</ispartof><rights>2010, Mary Ann Liebert, Inc.</rights><rights>COPYRIGHT 2010 Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-b4b27ecd106d5b20f8f70561888b6b251533da523ac31e2febd6f7f1e08f3f713</citedby><cites>FETCH-LOGICAL-c403t-b4b27ecd106d5b20f8f70561888b6b251533da523ac31e2febd6f7f1e08f3f713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20932182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oron, U.</creatorcontrib><creatorcontrib>Maltz, L.</creatorcontrib><creatorcontrib>Tuby, H.</creatorcontrib><creatorcontrib>Sorin, V.</creatorcontrib><creatorcontrib>Czerniak, A.</creatorcontrib><title>Enhanced Liver Regeneration Following Acute Hepatectomy by Low-Level Laser Therapy</title><title>Photomedicine and laser surgery</title><addtitle>Photomed Laser Surg</addtitle><description>Objective:
The aim of the present study was to investigate the effect of low-level laser therapy (LLLT) on liver regeneration following hepatectomy.
Background:
LLLT has been found to modulate various biological processes.
Materials and
Methods:
Twelve mature male rats were used. The liver was exposed, and 70% of it was excised. The rats were assigned randomly to two groups: control, non-laser treated, and experimental, laser-treated (diode [Ga-Al-As] laser 804 nm) group. For determination of newly formed blood vessels and proliferating cells, 5-Bromo-2′deoxyuridine (BrdU) was injected intraperitoneally. The rats were sacrificed 2 d post hepatectomy, and histological sections from each liver were processed for analysis of new blood-vessel formation using BrdU immunostaining kit. Mesenchymal stem cells (MSCs) were assessed using c-kit immunostaining. BrdU-labeled cells were counted as for estimation of newly formed hepatic cells.
Results:
It was found that the number of proliferating cells (BrdU positive cells) per area in the regenerating regions of the livers were significantly (
p
< 0.01) 2.6-fold higher in the laser-treated rats than in the control non-laser-treated rats. The density of the newly formed blood vessels and c-kit immunopositive cells in the regenerating area of the laser-treated livers was significantly (
p
< 0.01) 3.3- and 2.3-fold respectively higher than the control non-laser treated livers.
Conclusion:
It is concluded that LLLT following acute hepatectomy most probably stimulates a significant enhancement of liver regeneration conducive to both the formation of new hepatocytes and MSCs and angiogenesis in the regenerating liver.</description><subject>Acute Disease</subject><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Disease Models, Animal</subject><subject>Hepatectomy</subject><subject>Immunohistochemistry</subject><subject>Lasers in surgery</subject><subject>Lasers, Semiconductor - therapeutic use</subject><subject>Liver</subject><subject>Liver Regeneration - physiology</subject><subject>Liver Regeneration - radiation effects</subject><subject>Low-Level Light Therapy - methods</subject><subject>Male</subject><subject>Methods</subject><subject>Original Articles</subject><subject>Photomicrography</subject><subject>Postoperative care</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Reference Values</subject><subject>Regeneration</subject><subject>Technology application</subject><issn>1549-5418</issn><issn>1557-8550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctr3DAQh0VpadIkx16LoYeevNXDevi4hDwKhkJIz0KSR7sqtuRK3oT972uzSaFQKHOYYfh-w8CH0EeCNwSr9uu0TxuKcbuhkos36JxwLmvFOX67zk1b84aoM_ShlJ8Y01a27D06o7hllCh6jh5u4t5EB33VhSfI1QPsIEI2c0ixuk3DkJ5D3FVbd5ihuofJzODmNB4re6y69Fx38ARD1ZmyZB_3S3A6XqJ33gwFrl76Bfpxe_N4fV933---XW-72jWYzbVtLJXgeoJFzy3FXnmJuSBKKSss5YQz1htOmXGMAPVge-GlJ4CVZ14SdoG-nO5OOf06QJn1GIqDYTAR0qFoyVslhcJsIT-fyJ0ZQIfo05yNW2m9pQ0XggiyUpt_UEv1MAaXIviw7P8K1KeAy6mUDF5POYwmHzXBepWjFzl6laNXOQv_6eXhgx2h_0O_2lgAdgLWtYlxCGAhz_85-xtZLJoe</recordid><startdate>20101001</startdate><enddate>20101001</enddate><creator>Oron, U.</creator><creator>Maltz, L.</creator><creator>Tuby, H.</creator><creator>Sorin, V.</creator><creator>Czerniak, A.</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20101001</creationdate><title>Enhanced Liver Regeneration Following Acute Hepatectomy by Low-Level Laser Therapy</title><author>Oron, U. ; Maltz, L. ; Tuby, H. ; Sorin, V. ; Czerniak, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-b4b27ecd106d5b20f8f70561888b6b251533da523ac31e2febd6f7f1e08f3f713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acute Disease</topic><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Disease Models, Animal</topic><topic>Hepatectomy</topic><topic>Immunohistochemistry</topic><topic>Lasers in surgery</topic><topic>Lasers, Semiconductor - therapeutic use</topic><topic>Liver</topic><topic>Liver Regeneration - physiology</topic><topic>Liver Regeneration - radiation effects</topic><topic>Low-Level Light Therapy - methods</topic><topic>Male</topic><topic>Methods</topic><topic>Original Articles</topic><topic>Photomicrography</topic><topic>Postoperative care</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Reference Values</topic><topic>Regeneration</topic><topic>Technology application</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oron, U.</creatorcontrib><creatorcontrib>Maltz, L.</creatorcontrib><creatorcontrib>Tuby, H.</creatorcontrib><creatorcontrib>Sorin, V.</creatorcontrib><creatorcontrib>Czerniak, A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Photomedicine and laser surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oron, U.</au><au>Maltz, L.</au><au>Tuby, H.</au><au>Sorin, V.</au><au>Czerniak, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced Liver Regeneration Following Acute Hepatectomy by Low-Level Laser Therapy</atitle><jtitle>Photomedicine and laser surgery</jtitle><addtitle>Photomed Laser Surg</addtitle><date>2010-10-01</date><risdate>2010</risdate><volume>28</volume><issue>5</issue><spage>675</spage><epage>678</epage><pages>675-678</pages><issn>1549-5418</issn><eissn>1557-8550</eissn><abstract>Objective:
The aim of the present study was to investigate the effect of low-level laser therapy (LLLT) on liver regeneration following hepatectomy.
Background:
LLLT has been found to modulate various biological processes.
Materials and
Methods:
Twelve mature male rats were used. The liver was exposed, and 70% of it was excised. The rats were assigned randomly to two groups: control, non-laser treated, and experimental, laser-treated (diode [Ga-Al-As] laser 804 nm) group. For determination of newly formed blood vessels and proliferating cells, 5-Bromo-2′deoxyuridine (BrdU) was injected intraperitoneally. The rats were sacrificed 2 d post hepatectomy, and histological sections from each liver were processed for analysis of new blood-vessel formation using BrdU immunostaining kit. Mesenchymal stem cells (MSCs) were assessed using c-kit immunostaining. BrdU-labeled cells were counted as for estimation of newly formed hepatic cells.
Results:
It was found that the number of proliferating cells (BrdU positive cells) per area in the regenerating regions of the livers were significantly (
p
< 0.01) 2.6-fold higher in the laser-treated rats than in the control non-laser-treated rats. The density of the newly formed blood vessels and c-kit immunopositive cells in the regenerating area of the laser-treated livers was significantly (
p
< 0.01) 3.3- and 2.3-fold respectively higher than the control non-laser treated livers.
Conclusion:
It is concluded that LLLT following acute hepatectomy most probably stimulates a significant enhancement of liver regeneration conducive to both the formation of new hepatocytes and MSCs and angiogenesis in the regenerating liver.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>20932182</pmid><doi>10.1089/pho.2009.2756</doi><tpages>4</tpages></addata></record> |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Acute Disease Analysis of Variance Animals Disease Models, Animal Hepatectomy Immunohistochemistry Lasers in surgery Lasers, Semiconductor - therapeutic use Liver Liver Regeneration - physiology Liver Regeneration - radiation effects Low-Level Light Therapy - methods Male Methods Original Articles Photomicrography Postoperative care Random Allocation Rats Rats, Inbred Strains Reference Values Regeneration Technology application |
| title | Enhanced Liver Regeneration Following Acute Hepatectomy by Low-Level Laser Therapy |
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