Transepithelial calcium transport in the chick chorioallantoic membrane. II: Compartmentalization of calcium during uptake
Calcium transport from the eggshell to the developing chick embryo is carried out by the ectoderm cells of the chick chorioallantoic membrane. Primary cells isolated from chick chorioallantoic membrane ectoderm were used to analyze the subcellular distribution of 45Ca2+ accumulated from the extracel...
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| Veröffentlicht in: | Journal of cell science 1993-06, Vol.105 (2), p.381-388 |
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| creator | AKINS, R. E TUAN, R. S |
| description | Calcium transport from the eggshell to the developing chick embryo is carried out by the ectoderm cells of the chick chorioallantoic membrane. Primary cells isolated from chick chorioallantoic membrane ectoderm were used to analyze the subcellular distribution of 45Ca2+ accumulated from the extracellular medium. We present evidence suggesting that calcium may be sequestered into endosome-like vesicles during the initial phase of uptake. A combination of techniques were utilized to monitor calcium fluxes and calcium compartmentalization in the cultured chorioallantoic membrane cells: (1) fura-2 fluorescence was used to indicate cytosolic free calcium concentrations, (2) 45Ca2+ tracer was used to follow calcium accumulation in all cellular compartments, and (3) digitonin was used to differentially permeabilize subcellular membranes in order to localize 45Ca2+ by following tracer release profiles. Differences between cytosolic calcium flux and whole cell calcium accumulation suggested that the pathway of calcium uptake from the medium involves sequestration into an internal compartment separate from the cytosol. Kinetic analysis of the digitonin-mediated release of specific subcellular markers (lactate dehydrogenase, NAD-dependent isocitrate dehydrogenase, [3H]inulin, and [3H]-2-deoxyglucose) and preloaded 45Ca2+ indicated that calcium was localized in a compartment similar to endosomal vesicles. Our results are consistent with a transcytotic mechanism for chorioallantoic membrane calcium transport. |
| doi_str_mv | 10.1242/jcs.105.2.381 |
| format | Article |
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II: Compartmentalization of calcium during uptake</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Company of Biologists</source><creator>AKINS, R. E ; TUAN, R. S</creator><creatorcontrib>AKINS, R. E ; TUAN, R. S</creatorcontrib><description>Calcium transport from the eggshell to the developing chick embryo is carried out by the ectoderm cells of the chick chorioallantoic membrane. Primary cells isolated from chick chorioallantoic membrane ectoderm were used to analyze the subcellular distribution of 45Ca2+ accumulated from the extracellular medium. We present evidence suggesting that calcium may be sequestered into endosome-like vesicles during the initial phase of uptake. A combination of techniques were utilized to monitor calcium fluxes and calcium compartmentalization in the cultured chorioallantoic membrane cells: (1) fura-2 fluorescence was used to indicate cytosolic free calcium concentrations, (2) 45Ca2+ tracer was used to follow calcium accumulation in all cellular compartments, and (3) digitonin was used to differentially permeabilize subcellular membranes in order to localize 45Ca2+ by following tracer release profiles. Differences between cytosolic calcium flux and whole cell calcium accumulation suggested that the pathway of calcium uptake from the medium involves sequestration into an internal compartment separate from the cytosol. Kinetic analysis of the digitonin-mediated release of specific subcellular markers (lactate dehydrogenase, NAD-dependent isocitrate dehydrogenase, [3H]inulin, and [3H]-2-deoxyglucose) and preloaded 45Ca2+ indicated that calcium was localized in a compartment similar to endosomal vesicles. Our results are consistent with a transcytotic mechanism for chorioallantoic membrane calcium transport.</description><identifier>ISSN: 0021-9533</identifier><identifier>EISSN: 1477-9137</identifier><identifier>DOI: 10.1242/jcs.105.2.381</identifier><identifier>PMID: 8408272</identifier><identifier>CODEN: JNCSAI</identifier><language>eng</language><publisher>Cambridge: Company of Biologists</publisher><subject>Allantois - metabolism ; Allantois - ultrastructure ; Animals ; Biological and medical sciences ; Biological Transport, Active ; Biomarkers ; Calcium - metabolism ; Cell Compartmentation ; Cell Membrane Permeability - drug effects ; Cells, Cultured ; Chick Embryo - cytology ; Chick Embryo - metabolism ; Chorion - metabolism ; Chorion - ultrastructure ; Cytosol - metabolism ; Digitonin - pharmacology ; Ectoderm - cytology ; Ectoderm - metabolism ; Egg Shell - metabolism ; Embryology: invertebrates and vertebrates. Teratology ; Endocytosis ; Fetal membranes ; Fundamental and applied biological sciences. Psychology ; General aspects. Development. Fetal membranes ; Intracellular Membranes - drug effects ; Intracellular Membranes - metabolism ; Models, Biological</subject><ispartof>Journal of cell science, 1993-06, Vol.105 (2), p.381-388</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c267t-6ce257e531bc2fef58db5e96ffe741397945cace5636d80383e3eea32e5b566f3</citedby><cites>FETCH-LOGICAL-c267t-6ce257e531bc2fef58db5e96ffe741397945cace5636d80383e3eea32e5b566f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,3665,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3761436$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8408272$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>AKINS, R. E</creatorcontrib><creatorcontrib>TUAN, R. S</creatorcontrib><title>Transepithelial calcium transport in the chick chorioallantoic membrane. II: Compartmentalization of calcium during uptake</title><title>Journal of cell science</title><addtitle>J Cell Sci</addtitle><description>Calcium transport from the eggshell to the developing chick embryo is carried out by the ectoderm cells of the chick chorioallantoic membrane. Primary cells isolated from chick chorioallantoic membrane ectoderm were used to analyze the subcellular distribution of 45Ca2+ accumulated from the extracellular medium. We present evidence suggesting that calcium may be sequestered into endosome-like vesicles during the initial phase of uptake. A combination of techniques were utilized to monitor calcium fluxes and calcium compartmentalization in the cultured chorioallantoic membrane cells: (1) fura-2 fluorescence was used to indicate cytosolic free calcium concentrations, (2) 45Ca2+ tracer was used to follow calcium accumulation in all cellular compartments, and (3) digitonin was used to differentially permeabilize subcellular membranes in order to localize 45Ca2+ by following tracer release profiles. Differences between cytosolic calcium flux and whole cell calcium accumulation suggested that the pathway of calcium uptake from the medium involves sequestration into an internal compartment separate from the cytosol. Kinetic analysis of the digitonin-mediated release of specific subcellular markers (lactate dehydrogenase, NAD-dependent isocitrate dehydrogenase, [3H]inulin, and [3H]-2-deoxyglucose) and preloaded 45Ca2+ indicated that calcium was localized in a compartment similar to endosomal vesicles. Our results are consistent with a transcytotic mechanism for chorioallantoic membrane calcium transport.</description><subject>Allantois - metabolism</subject><subject>Allantois - ultrastructure</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Transport, Active</subject><subject>Biomarkers</subject><subject>Calcium - metabolism</subject><subject>Cell Compartmentation</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Cells, Cultured</subject><subject>Chick Embryo - cytology</subject><subject>Chick Embryo - metabolism</subject><subject>Chorion - metabolism</subject><subject>Chorion - ultrastructure</subject><subject>Cytosol - metabolism</subject><subject>Digitonin - pharmacology</subject><subject>Ectoderm - cytology</subject><subject>Ectoderm - metabolism</subject><subject>Egg Shell - metabolism</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Endocytosis</subject><subject>Fetal membranes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects. Development. Fetal membranes</subject><subject>Intracellular Membranes - drug effects</subject><subject>Intracellular Membranes - metabolism</subject><subject>Models, Biological</subject><issn>0021-9533</issn><issn>1477-9137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtLxDAURoMoOo4uXQpZiLvWPJqkdSeDjwHBja5LmrnRaNvUJF3orzeDw2xuLvkOH9yD0AUlJWUVu_k0saRElKzkNT1AC1opVTSUq0O0IITRohGcn6DTGD8JIYo16hgd1xWpmWIL9Psa9BhhcukDeqd7bHRv3DzgtP2ffEjYjTiH2Hw485WnD87rvtdj8s7gAYYuk1Di9foWr_ww6ZAGGJPu3a9Ozo_Y233pZg5ufMfzlPQXnKEjq_sI57t3id4e7l9XT8Xzy-N6dfdcGCZVKqQBJhQITjvDLFhRbzoBjbQWVEV5o5pKGG1ASC43NeE1Bw6gOQPRCSktX6Lr_94p-O8ZYmoHFw1sTwA_x1aJplY0Vy1R8Q-a4GMMYNspuEGHn5aSduu6za7zLlrWZteZv9wVz90Amz29k5vzq12uYzZgsyfj4h7jStKKS_4HB42KDw</recordid><startdate>199306</startdate><enddate>199306</enddate><creator>AKINS, R. E</creator><creator>TUAN, R. S</creator><general>Company of Biologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199306</creationdate><title>Transepithelial calcium transport in the chick chorioallantoic membrane. II: Compartmentalization of calcium during uptake</title><author>AKINS, R. E ; TUAN, R. S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c267t-6ce257e531bc2fef58db5e96ffe741397945cace5636d80383e3eea32e5b566f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Allantois - metabolism</topic><topic>Allantois - ultrastructure</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Transport, Active</topic><topic>Biomarkers</topic><topic>Calcium - metabolism</topic><topic>Cell Compartmentation</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>Cells, Cultured</topic><topic>Chick Embryo - cytology</topic><topic>Chick Embryo - metabolism</topic><topic>Chorion - metabolism</topic><topic>Chorion - ultrastructure</topic><topic>Cytosol - metabolism</topic><topic>Digitonin - pharmacology</topic><topic>Ectoderm - cytology</topic><topic>Ectoderm - metabolism</topic><topic>Egg Shell - metabolism</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Endocytosis</topic><topic>Fetal membranes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects. Development. Fetal membranes</topic><topic>Intracellular Membranes - drug effects</topic><topic>Intracellular Membranes - metabolism</topic><topic>Models, Biological</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>AKINS, R. E</creatorcontrib><creatorcontrib>TUAN, R. S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cell science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>AKINS, R. E</au><au>TUAN, R. S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transepithelial calcium transport in the chick chorioallantoic membrane. II: Compartmentalization of calcium during uptake</atitle><jtitle>Journal of cell science</jtitle><addtitle>J Cell Sci</addtitle><date>1993-06</date><risdate>1993</risdate><volume>105</volume><issue>2</issue><spage>381</spage><epage>388</epage><pages>381-388</pages><issn>0021-9533</issn><eissn>1477-9137</eissn><coden>JNCSAI</coden><abstract>Calcium transport from the eggshell to the developing chick embryo is carried out by the ectoderm cells of the chick chorioallantoic membrane. Primary cells isolated from chick chorioallantoic membrane ectoderm were used to analyze the subcellular distribution of 45Ca2+ accumulated from the extracellular medium. We present evidence suggesting that calcium may be sequestered into endosome-like vesicles during the initial phase of uptake. A combination of techniques were utilized to monitor calcium fluxes and calcium compartmentalization in the cultured chorioallantoic membrane cells: (1) fura-2 fluorescence was used to indicate cytosolic free calcium concentrations, (2) 45Ca2+ tracer was used to follow calcium accumulation in all cellular compartments, and (3) digitonin was used to differentially permeabilize subcellular membranes in order to localize 45Ca2+ by following tracer release profiles. Differences between cytosolic calcium flux and whole cell calcium accumulation suggested that the pathway of calcium uptake from the medium involves sequestration into an internal compartment separate from the cytosol. Kinetic analysis of the digitonin-mediated release of specific subcellular markers (lactate dehydrogenase, NAD-dependent isocitrate dehydrogenase, [3H]inulin, and [3H]-2-deoxyglucose) and preloaded 45Ca2+ indicated that calcium was localized in a compartment similar to endosomal vesicles. Our results are consistent with a transcytotic mechanism for chorioallantoic membrane calcium transport.</abstract><cop>Cambridge</cop><pub>Company of Biologists</pub><pmid>8408272</pmid><doi>10.1242/jcs.105.2.381</doi><tpages>8</tpages></addata></record> |
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| subjects | Allantois - metabolism Allantois - ultrastructure Animals Biological and medical sciences Biological Transport, Active Biomarkers Calcium - metabolism Cell Compartmentation Cell Membrane Permeability - drug effects Cells, Cultured Chick Embryo - cytology Chick Embryo - metabolism Chorion - metabolism Chorion - ultrastructure Cytosol - metabolism Digitonin - pharmacology Ectoderm - cytology Ectoderm - metabolism Egg Shell - metabolism Embryology: invertebrates and vertebrates. Teratology Endocytosis Fetal membranes Fundamental and applied biological sciences. Psychology General aspects. Development. Fetal membranes Intracellular Membranes - drug effects Intracellular Membranes - metabolism Models, Biological |
| title | Transepithelial calcium transport in the chick chorioallantoic membrane. II: Compartmentalization of calcium during uptake |
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