Gene therapy by intramuscular injection of plasmid DNA: studies on firefly luciferase gene expression in mice

Direct injection of nonviral, covalently closed circular plasmid DNA into muscle results in expression of the DNA in myofiber cells. We have examined the expression of firefly luciferase DNA constructs injected into adult murine skeletal muscle. Considerable variation in luciferase enzyme expression...

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Veröffentlicht in:	Human gene therapy 1993-08, Vol.4 (4), p.419-431
Hauptverfasser:	Manthorpe, M, Cornefert-Jensen, F, Hartikka, J, Felgner, J, Rundell, A, Margalith, M, Dwarki, V
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Blotting, Southern Cloning, Molecular Coleoptera - enzymology Coleoptera - genetics Cytomegalovirus - genetics Female Gene Expression Regulation, Enzymologic Genetic Therapy - methods Injections, Intramuscular Kinetics Luciferases - genetics Luciferases - metabolism Mice Molecular Sequence Data Plasmids - administration & dosage Promoter Regions, Genetic
Online-Zugang:	Volltext
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