Amplification and sequencing of variable regions in bacterial 23S ribosomal RNA genes with conserved primer sequences
Published bacterial 23S ribosomal RNA sequences were aligned, and universally conserved regions flanking highly variable regions were looked for. In strategically positioned conserved regions, six oligonucleotides suitable for polymerase chain reaction (PCR) and sequencing were designed, allowing fa...
Gespeichert in:
| Veröffentlicht in: | Current microbiology 1993-09, Vol.27 (3), p.147-151 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 151 |
|---|---|
| container_issue | 3 |
| container_start_page | 147 |
| container_title | Current microbiology |
| container_volume | 27 |
| creator | VAN CAMP, G CHAPELLE, S DE WACHTER, R |
| description | Published bacterial 23S ribosomal RNA sequences were aligned, and universally conserved regions flanking highly variable regions were looked for. In strategically positioned conserved regions, six oligonucleotides suitable for polymerase chain reaction (PCR) and sequencing were designed, allowing fast sequencing of four of the most variable 23S rRNA regions. Two other primers were designed for PCR amplification of nearly complete 23S rRNA genes. All these primers successfully amplified fragments of 23S rRNA genes from seven unrelated bacteria. Four primers were used to determine 938 bp of sequence for Campylobacter jejuni subsp. jejuni. These results indicate that the oligonucleotide sequences presented here are useful for PCR amplification and sequence determination of variable 23S rRNA regions for a broad variety of eubacterial species. |
| doi_str_mv | 10.1007/BF01576012 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75981160</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>75981160</sourcerecordid><originalsourceid>FETCH-LOGICAL-c378t-9555374ec4c903556eed52e66b8aee3b404c1f4681e0003a4f0381480aa000a23</originalsourceid><addsrcrecordid>eNqF0UtLAzEQB_AgitbqxbuQg3gQVieb1-6xFl9QFHycl2w6WyP7qMm24rc3pbUePYXJ_PiHzBBywuCSAeir61tgUitg6Q4ZMMHTBPKc7ZIBcMGTTEl2QA5D-IAocmD7ZF-rnHFQA7IYNfPaVc6a3nUtNe2UBvxcYGtdO6NdRZfGO1PWSD3OogjUtbQ0tsd4XdOUv1Dvyi50TayeH0d0hi0G-uX6d2ojR7_EKZ1716D_TcZwRPYqUwc83pxD8nZ78zq-TyZPdw_j0SSxXGd9kkspuRZohc2BS6kQpzJFpcrMIPJSgLCsEipjCADciAp4xkQGxsTapHxIzte5c9_Fp0NfNC5YrGvTYrcIhZZ5xpiCfyFTWijJVYQXa2h9F4LHqlj9zfjvgkGxWkbxt4yITzepi7LB6ZZuph_7Z5u-CdbUlTdx7GHLuNZCcOA_RjuQWA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16746536</pqid></control><display><type>article</type><title>Amplification and sequencing of variable regions in bacterial 23S ribosomal RNA genes with conserved primer sequences</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>VAN CAMP, G ; CHAPELLE, S ; DE WACHTER, R</creator><creatorcontrib>VAN CAMP, G ; CHAPELLE, S ; DE WACHTER, R</creatorcontrib><description>Published bacterial 23S ribosomal RNA sequences were aligned, and universally conserved regions flanking highly variable regions were looked for. In strategically positioned conserved regions, six oligonucleotides suitable for polymerase chain reaction (PCR) and sequencing were designed, allowing fast sequencing of four of the most variable 23S rRNA regions. Two other primers were designed for PCR amplification of nearly complete 23S rRNA genes. All these primers successfully amplified fragments of 23S rRNA genes from seven unrelated bacteria. Four primers were used to determine 938 bp of sequence for Campylobacter jejuni subsp. jejuni. These results indicate that the oligonucleotide sequences presented here are useful for PCR amplification and sequence determination of variable 23S rRNA regions for a broad variety of eubacterial species.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/BF01576012</identifier><identifier>PMID: 7691306</identifier><identifier>CODEN: CUMIDD</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Bacteriology ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Conserved Sequence ; DNA Primers - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genetics ; Microbiology ; Molecular Sequence Data ; Polymerase Chain Reaction ; RNA, Bacterial - genetics ; RNA, Ribosomal, 23S - genetics</subject><ispartof>Current microbiology, 1993-09, Vol.27 (3), p.147-151</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-9555374ec4c903556eed52e66b8aee3b404c1f4681e0003a4f0381480aa000a23</citedby><cites>FETCH-LOGICAL-c378t-9555374ec4c903556eed52e66b8aee3b404c1f4681e0003a4f0381480aa000a23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3774430$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7691306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VAN CAMP, G</creatorcontrib><creatorcontrib>CHAPELLE, S</creatorcontrib><creatorcontrib>DE WACHTER, R</creatorcontrib><title>Amplification and sequencing of variable regions in bacterial 23S ribosomal RNA genes with conserved primer sequences</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><description>Published bacterial 23S ribosomal RNA sequences were aligned, and universally conserved regions flanking highly variable regions were looked for. In strategically positioned conserved regions, six oligonucleotides suitable for polymerase chain reaction (PCR) and sequencing were designed, allowing fast sequencing of four of the most variable 23S rRNA regions. Two other primers were designed for PCR amplification of nearly complete 23S rRNA genes. All these primers successfully amplified fragments of 23S rRNA genes from seven unrelated bacteria. Four primers were used to determine 938 bp of sequence for Campylobacter jejuni subsp. jejuni. These results indicate that the oligonucleotide sequences presented here are useful for PCR amplification and sequence determination of variable 23S rRNA regions for a broad variety of eubacterial species.</description><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Conserved Sequence</subject><subject>DNA Primers - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Ribosomal, 23S - genetics</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UtLAzEQB_AgitbqxbuQg3gQVieb1-6xFl9QFHycl2w6WyP7qMm24rc3pbUePYXJ_PiHzBBywuCSAeir61tgUitg6Q4ZMMHTBPKc7ZIBcMGTTEl2QA5D-IAocmD7ZF-rnHFQA7IYNfPaVc6a3nUtNe2UBvxcYGtdO6NdRZfGO1PWSD3OogjUtbQ0tsd4XdOUv1Dvyi50TayeH0d0hi0G-uX6d2ojR7_EKZ1716D_TcZwRPYqUwc83pxD8nZ78zq-TyZPdw_j0SSxXGd9kkspuRZohc2BS6kQpzJFpcrMIPJSgLCsEipjCADciAp4xkQGxsTapHxIzte5c9_Fp0NfNC5YrGvTYrcIhZZ5xpiCfyFTWijJVYQXa2h9F4LHqlj9zfjvgkGxWkbxt4yITzepi7LB6ZZuph_7Z5u-CdbUlTdx7GHLuNZCcOA_RjuQWA</recordid><startdate>19930901</startdate><enddate>19930901</enddate><creator>VAN CAMP, G</creator><creator>CHAPELLE, S</creator><creator>DE WACHTER, R</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19930901</creationdate><title>Amplification and sequencing of variable regions in bacterial 23S ribosomal RNA genes with conserved primer sequences</title><author>VAN CAMP, G ; CHAPELLE, S ; DE WACHTER, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-9555374ec4c903556eed52e66b8aee3b404c1f4681e0003a4f0381480aa000a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Conserved Sequence</topic><topic>DNA Primers - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Ribosomal, 23S - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VAN CAMP, G</creatorcontrib><creatorcontrib>CHAPELLE, S</creatorcontrib><creatorcontrib>DE WACHTER, R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>VAN CAMP, G</au><au>CHAPELLE, S</au><au>DE WACHTER, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amplification and sequencing of variable regions in bacterial 23S ribosomal RNA genes with conserved primer sequences</atitle><jtitle>Current microbiology</jtitle><addtitle>Curr Microbiol</addtitle><date>1993-09-01</date><risdate>1993</risdate><volume>27</volume><issue>3</issue><spage>147</spage><epage>151</epage><pages>147-151</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><coden>CUMIDD</coden><abstract>Published bacterial 23S ribosomal RNA sequences were aligned, and universally conserved regions flanking highly variable regions were looked for. In strategically positioned conserved regions, six oligonucleotides suitable for polymerase chain reaction (PCR) and sequencing were designed, allowing fast sequencing of four of the most variable 23S rRNA regions. Two other primers were designed for PCR amplification of nearly complete 23S rRNA genes. All these primers successfully amplified fragments of 23S rRNA genes from seven unrelated bacteria. Four primers were used to determine 938 bp of sequence for Campylobacter jejuni subsp. jejuni. These results indicate that the oligonucleotide sequences presented here are useful for PCR amplification and sequence determination of variable 23S rRNA regions for a broad variety of eubacterial species.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>7691306</pmid><doi>10.1007/BF01576012</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0343-8651 |
| ispartof | Current microbiology, 1993-09, Vol.27 (3), p.147-151 |
| issn | 0343-8651 1432-0991 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75981160 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Bacteriology Base Sequence Biological and medical sciences Biotechnology Conserved Sequence DNA Primers - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetics Microbiology Molecular Sequence Data Polymerase Chain Reaction RNA, Bacterial - genetics RNA, Ribosomal, 23S - genetics |
| title | Amplification and sequencing of variable regions in bacterial 23S ribosomal RNA genes with conserved primer sequences |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T19%3A17%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Amplification%20and%20sequencing%20of%20variable%20regions%20in%20bacterial%2023S%20ribosomal%20RNA%20genes%20with%20conserved%20primer%20sequences&rft.jtitle=Current%20microbiology&rft.au=VAN%20CAMP,%20G&rft.date=1993-09-01&rft.volume=27&rft.issue=3&rft.spage=147&rft.epage=151&rft.pages=147-151&rft.issn=0343-8651&rft.eissn=1432-0991&rft.coden=CUMIDD&rft_id=info:doi/10.1007/BF01576012&rft_dat=%3Cproquest_cross%3E75981160%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16746536&rft_id=info:pmid/7691306&rfr_iscdi=true |