Inhibition of 3 β-hydroxysteroid dehydrogenase (3 β-HSD) activity in the granulosa cells of mouse ovarian follicles by follicular fluid peptide
A protein fraction (GF 2) was purified from sheep ovarian follicular fluid. Its action on 3 β-HSD activity in the mouse granulosa cells was measured in an in vitro system. The fraction (GF 2) caused dose-dependent cep emotion of the 3 β-HSD activity in granulosa cells and progesterone in the spent m...
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| Veröffentlicht in: | Steroids 1984-12, Vol.44 (6), p.497-509 |
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| creator | Kadam, A.L. Moodbidri, S.B. Nandedkar, T.D. |
| description | A protein fraction (GF
2) was purified from sheep ovarian follicular fluid. Its action on 3 β-HSD activity in the mouse granulosa cells was measured in an
in
vitro system. The fraction (GF
2) caused dose-dependent cep emotion of the 3 β-HSD activity in granulosa cells and progesterone in the spent medium. A maximum inhibition of the activity was achieved after 30 min incubation of the granulosa cells with the GF
2 fraction. Further purified HPLC fraction (Fr
1) also inhibited 3 β-HSD activity.
In
vivo administration of the GF
2 fraction in normal cycling female mice also decreased the 3 β-HSD activity in the granulosa cells of the ovarian follicles and plasma progesterone levels indicating the GF
2 fraction to be a 3 β-HSD inhibitor. |
| doi_str_mv | 10.1016/S0039-128X(84)80031-8 |
| format | Article |
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2) was purified from sheep ovarian follicular fluid. Its action on 3 β-HSD activity in the mouse granulosa cells was measured in an
in
vitro system. The fraction (GF
2) caused dose-dependent cep emotion of the 3 β-HSD activity in granulosa cells and progesterone in the spent medium. A maximum inhibition of the activity was achieved after 30 min incubation of the granulosa cells with the GF
2 fraction. Further purified HPLC fraction (Fr
1) also inhibited 3 β-HSD activity.
In
vivo administration of the GF
2 fraction in normal cycling female mice also decreased the 3 β-HSD activity in the granulosa cells of the ovarian follicles and plasma progesterone levels indicating the GF
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2) was purified from sheep ovarian follicular fluid. Its action on 3 β-HSD activity in the mouse granulosa cells was measured in an
in
vitro system. The fraction (GF
2) caused dose-dependent cep emotion of the 3 β-HSD activity in granulosa cells and progesterone in the spent medium. A maximum inhibition of the activity was achieved after 30 min incubation of the granulosa cells with the GF
2 fraction. Further purified HPLC fraction (Fr
1) also inhibited 3 β-HSD activity.
In
vivo administration of the GF
2 fraction in normal cycling female mice also decreased the 3 β-HSD activity in the granulosa cells of the ovarian follicles and plasma progesterone levels indicating the GF
2 fraction to be a 3 β-HSD inhibitor.</description><subject>3-Hydroxysteroid Dehydrogenases - antagonists & inhibitors</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Granulosa Cells - enzymology</subject><subject>Hormone metabolism and regulation</subject><subject>Hot Temperature</subject><subject>Kinetics</subject><subject>Mammalian female genital system</subject><subject>Mice</subject><subject>Ovarian Follicle - physiology</subject><subject>Progesterone Reductase - antagonists & inhibitors</subject><subject>Proteins - isolation & purification</subject><subject>Proteins - pharmacology</subject><subject>Sheep</subject><subject>Tissue Extracts - pharmacology</subject><subject>Trypsin</subject><subject>Vertebrates: reproduction</subject><issn>0039-128X</issn><issn>1878-5867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxi0EKkvhESr5gFB7CNhJHNsnVJU_rVSJQ0HiZjn2uGvkjRc7WZHH4FV4EJ4J7260V07WeH4z8-n7ELqg5C0ltHv3QEgjK1qL75eivRKlopV4glZUcFEx0fGnaHVCnqMXOf8ghHSNrM_QWcekZG2zQr_vhrXv_ejjgKPDDf77p1rPNsVfcx4hRW-xhcPHIww6A748ILcPH66wNqPf-XHGfsDjGvBj0sMUYtbYQAh5v28TpzITdzp5PWAXQ_AmQMb9vBRT0Am7MJU7W9iO3sJL9MzpkOHV8p6jb58-fr25re6_fL67ub6vTCPkWLFa2Nb2rtHCcsk6V9cchOSu7nlTU2ZrK2RLnexrZgwBbQ2lPQhi2o6JnjTn6M1x7zbFnxPkUW183gvXAxTVijMpSNfSArIjaFLMOYFT2-Q3Os2KErWPQh2iUHuflWjVIQolytzFcmDqN2BPU4v3pf966etsdHDFPePzCROMs6blBXt_xKCYsfOQVDYeBgPWJzCjstH_R8g_ezupVQ</recordid><startdate>198412</startdate><enddate>198412</enddate><creator>Kadam, A.L.</creator><creator>Moodbidri, S.B.</creator><creator>Nandedkar, T.D.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198412</creationdate><title>Inhibition of 3 β-hydroxysteroid dehydrogenase (3 β-HSD) activity in the granulosa cells of mouse ovarian follicles by follicular fluid peptide</title><author>Kadam, A.L. ; Moodbidri, S.B. ; Nandedkar, T.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-528d4dbf3a8d7956f227e897f2b73215d2d8941f9b25cc0eadc11be80c4658b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>3-Hydroxysteroid Dehydrogenases - antagonists & inhibitors</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Granulosa Cells - enzymology</topic><topic>Hormone metabolism and regulation</topic><topic>Hot Temperature</topic><topic>Kinetics</topic><topic>Mammalian female genital system</topic><topic>Mice</topic><topic>Ovarian Follicle - physiology</topic><topic>Progesterone Reductase - antagonists & inhibitors</topic><topic>Proteins - isolation & purification</topic><topic>Proteins - pharmacology</topic><topic>Sheep</topic><topic>Tissue Extracts - pharmacology</topic><topic>Trypsin</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kadam, A.L.</creatorcontrib><creatorcontrib>Moodbidri, S.B.</creatorcontrib><creatorcontrib>Nandedkar, T.D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Steroids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kadam, A.L.</au><au>Moodbidri, S.B.</au><au>Nandedkar, T.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of 3 β-hydroxysteroid dehydrogenase (3 β-HSD) activity in the granulosa cells of mouse ovarian follicles by follicular fluid peptide</atitle><jtitle>Steroids</jtitle><addtitle>Steroids</addtitle><date>1984-12</date><risdate>1984</risdate><volume>44</volume><issue>6</issue><spage>497</spage><epage>509</epage><pages>497-509</pages><issn>0039-128X</issn><eissn>1878-5867</eissn><coden>STEDAM</coden><abstract>A protein fraction (GF
2) was purified from sheep ovarian follicular fluid. Its action on 3 β-HSD activity in the mouse granulosa cells was measured in an
in
vitro system. The fraction (GF
2) caused dose-dependent cep emotion of the 3 β-HSD activity in granulosa cells and progesterone in the spent medium. A maximum inhibition of the activity was achieved after 30 min incubation of the granulosa cells with the GF
2 fraction. Further purified HPLC fraction (Fr
1) also inhibited 3 β-HSD activity.
In
vivo administration of the GF
2 fraction in normal cycling female mice also decreased the 3 β-HSD activity in the granulosa cells of the ovarian follicles and plasma progesterone levels indicating the GF
2 fraction to be a 3 β-HSD inhibitor.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>6599543</pmid><doi>10.1016/S0039-128X(84)80031-8</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0039-128X |
| ispartof | Steroids, 1984-12, Vol.44 (6), p.497-509 |
| issn | 0039-128X 1878-5867 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75980641 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 3-Hydroxysteroid Dehydrogenases - antagonists & inhibitors Animals Biological and medical sciences Female Fundamental and applied biological sciences. Psychology Granulosa Cells - enzymology Hormone metabolism and regulation Hot Temperature Kinetics Mammalian female genital system Mice Ovarian Follicle - physiology Progesterone Reductase - antagonists & inhibitors Proteins - isolation & purification Proteins - pharmacology Sheep Tissue Extracts - pharmacology Trypsin Vertebrates: reproduction |
| title | Inhibition of 3 β-hydroxysteroid dehydrogenase (3 β-HSD) activity in the granulosa cells of mouse ovarian follicles by follicular fluid peptide |
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