Cytotoxic T cells in HIV2 seropositive Gambians. Identification of a virus-specific MHC-restricted peptide epitope
A preliminary study of gag-specific, MHC-restricted CD8+ CTL has been performed in nine Gambian patients infected with HIV2. Such CTL were present in at least 55% of patients in fresh peripheral blood mononuclear cells without the requirement for in vitro restimulation. We have identified a nonamer...
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| Veröffentlicht in: | The Journal of immunology (1950) 1993-09, Vol.151 (6), p.3361-3369 |
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| container_end_page | 3369 |
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| container_issue | 6 |
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| container_title | The Journal of immunology (1950) |
| container_volume | 151 |
| creator | Gotch, F McAdam, SN Allsopp, CE Gallimore, A Elvin, J Kieny, MP Hill, AV McMichael, AJ Whittle, HC |
| description | A preliminary study of gag-specific, MHC-restricted CD8+ CTL has been performed in nine Gambian patients infected with HIV2. Such CTL were present in at least 55% of patients in fresh peripheral blood mononuclear cells without the requirement for in vitro restimulation. We have identified a nonamer peptide from HIV2 gag that is recognized by CD8+ HLA-B53 CTL using an amino acid sequence motif predicted from analysis of endogenous peptides eluted from HLA-B53 molecules. This peptide, from an HIV2/SIV conserved sequence, has previously been reported to be recognized by CTL from non-human primates vaccinated with recombinant vaccinia virus expressing the gag protein of SIV or infected with SIV virus. HLA-B53-restricted, HIV2 gag-specific CTL did not recognize target cells expressing HIV1 gag proteins, indicating that no cellular cross protection to HIV1 could be expected in this case. |
| doi_str_mv | 10.4049/jimmunol.151.6.3361 |
| format | Article |
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Identification of a virus-specific MHC-restricted peptide epitope</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Gotch, F ; McAdam, SN ; Allsopp, CE ; Gallimore, A ; Elvin, J ; Kieny, MP ; Hill, AV ; McMichael, AJ ; Whittle, HC</creator><creatorcontrib>Gotch, F ; McAdam, SN ; Allsopp, CE ; Gallimore, A ; Elvin, J ; Kieny, MP ; Hill, AV ; McMichael, AJ ; Whittle, HC</creatorcontrib><description>A preliminary study of gag-specific, MHC-restricted CD8+ CTL has been performed in nine Gambian patients infected with HIV2. Such CTL were present in at least 55% of patients in fresh peripheral blood mononuclear cells without the requirement for in vitro restimulation. We have identified a nonamer peptide from HIV2 gag that is recognized by CD8+ HLA-B53 CTL using an amino acid sequence motif predicted from analysis of endogenous peptides eluted from HLA-B53 molecules. This peptide, from an HIV2/SIV conserved sequence, has previously been reported to be recognized by CTL from non-human primates vaccinated with recombinant vaccinia virus expressing the gag protein of SIV or infected with SIV virus. HLA-B53-restricted, HIV2 gag-specific CTL did not recognize target cells expressing HIV1 gag proteins, indicating that no cellular cross protection to HIV1 could be expected in this case.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.151.6.3361</identifier><identifier>PMID: 7690804</identifier><language>eng</language><publisher>United States: Am Assoc Immnol</publisher><subject>AIDS/HIV ; Amino Acid Sequence ; Cytotoxicity, Immunologic ; Epitopes ; Gambia ; Gene Products, gag - chemistry ; Gene Products, gag - immunology ; HIV Infections - immunology ; HIV Seropositivity - immunology ; HIV-2 - immunology ; human immunodeficiency virus 2 ; Humans ; Immunity, Cellular ; Major Histocompatibility Complex ; Molecular Sequence Data ; Peptides - immunology ; Recombinant Proteins ; T-Lymphocytes, Cytotoxic - immunology</subject><ispartof>The Journal of immunology (1950), 1993-09, Vol.151 (6), p.3361-3369</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3231-a8b82953aa4549b612d15fb9472636c3013e16314d7c0882c80d78b2173f4ac83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7690804$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gotch, F</creatorcontrib><creatorcontrib>McAdam, SN</creatorcontrib><creatorcontrib>Allsopp, CE</creatorcontrib><creatorcontrib>Gallimore, A</creatorcontrib><creatorcontrib>Elvin, J</creatorcontrib><creatorcontrib>Kieny, MP</creatorcontrib><creatorcontrib>Hill, AV</creatorcontrib><creatorcontrib>McMichael, AJ</creatorcontrib><creatorcontrib>Whittle, HC</creatorcontrib><title>Cytotoxic T cells in HIV2 seropositive Gambians. Identification of a virus-specific MHC-restricted peptide epitope</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>A preliminary study of gag-specific, MHC-restricted CD8+ CTL has been performed in nine Gambian patients infected with HIV2. Such CTL were present in at least 55% of patients in fresh peripheral blood mononuclear cells without the requirement for in vitro restimulation. We have identified a nonamer peptide from HIV2 gag that is recognized by CD8+ HLA-B53 CTL using an amino acid sequence motif predicted from analysis of endogenous peptides eluted from HLA-B53 molecules. This peptide, from an HIV2/SIV conserved sequence, has previously been reported to be recognized by CTL from non-human primates vaccinated with recombinant vaccinia virus expressing the gag protein of SIV or infected with SIV virus. HLA-B53-restricted, HIV2 gag-specific CTL did not recognize target cells expressing HIV1 gag proteins, indicating that no cellular cross protection to HIV1 could be expected in this case.</description><subject>AIDS/HIV</subject><subject>Amino Acid Sequence</subject><subject>Cytotoxicity, Immunologic</subject><subject>Epitopes</subject><subject>Gambia</subject><subject>Gene Products, gag - chemistry</subject><subject>Gene Products, gag - immunology</subject><subject>HIV Infections - immunology</subject><subject>HIV Seropositivity - immunology</subject><subject>HIV-2 - immunology</subject><subject>human immunodeficiency virus 2</subject><subject>Humans</subject><subject>Immunity, Cellular</subject><subject>Major Histocompatibility Complex</subject><subject>Molecular Sequence Data</subject><subject>Peptides - immunology</subject><subject>Recombinant Proteins</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhq0KVLaFX4CQfIJTgr9iO0e0Kt2VWvVSuFqOM2ldJXGwnS799yTaLeLGaQ7vh2bmQegjJaUgov765IdhHkNf0oqWsuRc0jO0oVVFCimJfIM2hDBWUCXVO3SR0hMhRBImztG5kjXRRGxQ3L7kkMNv7_A9dtD3CfsR7_Y_GU4QwxSSz_4Z8LUdGm_HVOJ9C2P2nXc2-zDi0GGLn32cU5EmcKuAb3fbIkLK0bsMLZ5gyr4FDJPPYYL36G1n-wQfTvMS_fh-db_dFTd31_vtt5vCccZpYXWjWV1xa0Ul6kZS1tKqa2qhmOTScUI5UMmpaJUjWjOnSat0w6jinbBO80v0-dg7xfBrXtYxg0_riXaEMCejqloKwv9vpFItP9WrkR-NLoaUInRmin6w8cVQYlYk5hWJWZAYaVYkS-rTqX5uBmj_Zk4MFv3LUX_0D48HH8Gkwfb94qbmcDj80_QHHlmWuw</recordid><startdate>19930915</startdate><enddate>19930915</enddate><creator>Gotch, F</creator><creator>McAdam, SN</creator><creator>Allsopp, CE</creator><creator>Gallimore, A</creator><creator>Elvin, J</creator><creator>Kieny, MP</creator><creator>Hill, AV</creator><creator>McMichael, AJ</creator><creator>Whittle, HC</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19930915</creationdate><title>Cytotoxic T cells in HIV2 seropositive Gambians. Identification of a virus-specific MHC-restricted peptide epitope</title><author>Gotch, F ; McAdam, SN ; Allsopp, CE ; Gallimore, A ; Elvin, J ; Kieny, MP ; Hill, AV ; McMichael, AJ ; Whittle, HC</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3231-a8b82953aa4549b612d15fb9472636c3013e16314d7c0882c80d78b2173f4ac83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>AIDS/HIV</topic><topic>Amino Acid Sequence</topic><topic>Cytotoxicity, Immunologic</topic><topic>Epitopes</topic><topic>Gambia</topic><topic>Gene Products, gag - chemistry</topic><topic>Gene Products, gag - immunology</topic><topic>HIV Infections - immunology</topic><topic>HIV Seropositivity - immunology</topic><topic>HIV-2 - immunology</topic><topic>human immunodeficiency virus 2</topic><topic>Humans</topic><topic>Immunity, Cellular</topic><topic>Major Histocompatibility Complex</topic><topic>Molecular Sequence Data</topic><topic>Peptides - immunology</topic><topic>Recombinant Proteins</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gotch, F</creatorcontrib><creatorcontrib>McAdam, SN</creatorcontrib><creatorcontrib>Allsopp, CE</creatorcontrib><creatorcontrib>Gallimore, A</creatorcontrib><creatorcontrib>Elvin, J</creatorcontrib><creatorcontrib>Kieny, MP</creatorcontrib><creatorcontrib>Hill, AV</creatorcontrib><creatorcontrib>McMichael, AJ</creatorcontrib><creatorcontrib>Whittle, HC</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gotch, F</au><au>McAdam, SN</au><au>Allsopp, CE</au><au>Gallimore, A</au><au>Elvin, J</au><au>Kieny, MP</au><au>Hill, AV</au><au>McMichael, AJ</au><au>Whittle, HC</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytotoxic T cells in HIV2 seropositive Gambians. Identification of a virus-specific MHC-restricted peptide epitope</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1993-09-15</date><risdate>1993</risdate><volume>151</volume><issue>6</issue><spage>3361</spage><epage>3369</epage><pages>3361-3369</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>A preliminary study of gag-specific, MHC-restricted CD8+ CTL has been performed in nine Gambian patients infected with HIV2. Such CTL were present in at least 55% of patients in fresh peripheral blood mononuclear cells without the requirement for in vitro restimulation. We have identified a nonamer peptide from HIV2 gag that is recognized by CD8+ HLA-B53 CTL using an amino acid sequence motif predicted from analysis of endogenous peptides eluted from HLA-B53 molecules. This peptide, from an HIV2/SIV conserved sequence, has previously been reported to be recognized by CTL from non-human primates vaccinated with recombinant vaccinia virus expressing the gag protein of SIV or infected with SIV virus. HLA-B53-restricted, HIV2 gag-specific CTL did not recognize target cells expressing HIV1 gag proteins, indicating that no cellular cross protection to HIV1 could be expected in this case.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>7690804</pmid><doi>10.4049/jimmunol.151.6.3361</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 1993-09, Vol.151 (6), p.3361-3369 |
| issn | 0022-1767 1550-6606 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | AIDS/HIV Amino Acid Sequence Cytotoxicity, Immunologic Epitopes Gambia Gene Products, gag - chemistry Gene Products, gag - immunology HIV Infections - immunology HIV Seropositivity - immunology HIV-2 - immunology human immunodeficiency virus 2 Humans Immunity, Cellular Major Histocompatibility Complex Molecular Sequence Data Peptides - immunology Recombinant Proteins T-Lymphocytes, Cytotoxic - immunology |
| title | Cytotoxic T cells in HIV2 seropositive Gambians. Identification of a virus-specific MHC-restricted peptide epitope |
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