A recombinant human calcitonin receptor functions as an extracellular calcium sensor

Calcitonin, a peptide hormone active in calcium homeostasis, is used in the treatment of bone loss disorders because it inhibits osteoclast function. A human calcitonin receptor was cloned and expressed in baby hamster kidney cells. Three independent stable transfectants respond to calcitonin via in...

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Veröffentlicht in:The Journal of biological chemistry 1993-09, Vol.268 (27), p.19927-19930
Hauptverfasser: STROOP, S. D, THOMPSON, D. L, KUESTNER, R. E, MOORE, E. E
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container_end_page 19930
container_issue 27
container_start_page 19927
container_title The Journal of biological chemistry
container_volume 268
creator STROOP, S. D
THOMPSON, D. L
KUESTNER, R. E
MOORE, E. E
description Calcitonin, a peptide hormone active in calcium homeostasis, is used in the treatment of bone loss disorders because it inhibits osteoclast function. A human calcitonin receptor was cloned and expressed in baby hamster kidney cells. Three independent stable transfectants respond to calcitonin via increased intracellular calcium ([Ca2+]i) and cyclic adenosine 3',5'-monophosphate (cAMP). We made the interesting observation that these cells also respond to millimolar increases in extracellular calcium via a rapid and sustained elevation in [Ca2+]i, whereas three calcitonin receptor-negative baby hamster kidney cell lines, two of which express recombinant receptors related to the calcitonin receptor, show no sensitivity to changes in extracellular calcium. The increase of [Ca2+]i in response to both calcitonin and extracellular calcium is a function of the average number of calcitonin receptors per cell. These studies suggest a dual role for the calcitonin receptor as a hormone receptor and an extracellular calcium sensor.
doi_str_mv 10.1016/S0021-9258(20)80674-7
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subjects 8-Bromo Cyclic Adenosine Monophosphate - pharmacology
Animals
Biological and medical sciences
Calcitonin - metabolism
Calcitonin - pharmacology
Calcium - metabolism
Calcium - pharmacology
Cell Line
Cell Membrane - metabolism
Cell receptors
Cell structures and functions
Cloning, Molecular
Colforsin - pharmacology
Cricetinae
Cyclic AMP - metabolism
Cytosol - metabolism
Fundamental and applied biological sciences. Psychology
Glucagon - pharmacology
Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors
Humans
Kidney
Kinetics
Molecular and cellular biology
Receptors, Calcitonin
Receptors, Cell Surface - biosynthesis
Receptors, Cell Surface - metabolism
Recombinant Proteins - biosynthesis
Recombinant Proteins - metabolism
Time Factors
Transfection
title A recombinant human calcitonin receptor functions as an extracellular calcium sensor
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