Crystallization and Preliminary X-ray Analysis of the Di-Haem Cytochrome c Peroxidase from Pseudomonas aeruginosa
Cytochrome c 551 peroxidase is a periplasmic enzyme expressed in Pseudomas aeruginosa at low oxygen tensions. The glycosylated enzyme has been purified to homogeneity and crystallized by vapour diffusion techniques using polyethylene glycol 2000 as the precipitant in the presence of isopropanol. The...
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| Veröffentlicht in: | Journal of molecular biology 1993-08, Vol.232 (4), p.1208-1210 |
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| container_issue | 4 |
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| container_title | Journal of molecular biology |
| container_volume | 232 |
| creator | Fülöp, Vilmos Little, Richard Thompson, Adrian Greenwood, Colin Hajdu, Janos |
| description | Cytochrome
c
551 peroxidase is a periplasmic enzyme expressed in
Pseudomas aeruginosa at low oxygen tensions. The glycosylated enzyme has been purified to homogeneity and crystallized by vapour diffusion techniques using polyethylene glycol 2000 as the precipitant in the presence of isopropanol. The crystals belong to the trigonal space group
P 3
121 or
P3
221 with unit cell dimensions of
a =
b = 113·8 Å,
c = 72·0 Å. They are suitable for X-ray analysis and diffract to
d
min = 2·5 Å. There is one peroxidase molecule in the crystallographic asymmetric unit. |
| doi_str_mv | 10.1006/jmbi.1993.1472 |
| format | Article |
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c
551 peroxidase is a periplasmic enzyme expressed in
Pseudomas aeruginosa at low oxygen tensions. The glycosylated enzyme has been purified to homogeneity and crystallized by vapour diffusion techniques using polyethylene glycol 2000 as the precipitant in the presence of isopropanol. The crystals belong to the trigonal space group
P 3
121 or
P3
221 with unit cell dimensions of
a =
b = 113·8 Å,
c = 72·0 Å. They are suitable for X-ray analysis and diffract to
d
min = 2·5 Å. There is one peroxidase molecule in the crystallographic asymmetric unit.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1006/jmbi.1993.1472</identifier><identifier>PMID: 8396649</identifier><identifier>CODEN: JMOBAK</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Biological and medical sciences ; Crystalline structure ; crystallization ; cytochrome c551 peroxidase ; Cytochrome-c Peroxidase - chemistry ; Fundamental and applied biological sciences. Psychology ; Glycoproteins - chemistry ; glycosylation ; Hemeproteins - chemistry ; Molecular biophysics ; periplasmic protein ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - enzymology ; Structure in molecular biology ; X-Ray Diffraction</subject><ispartof>Journal of molecular biology, 1993-08, Vol.232 (4), p.1208-1210</ispartof><rights>1993 Academic Press</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c314t-18b383fd3024a9c0c25adb594e934a6c7b7b2f96e668829ba17fb8422e0e3a9b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/jmbi.1993.1472$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4888793$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8396649$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fülöp, Vilmos</creatorcontrib><creatorcontrib>Little, Richard</creatorcontrib><creatorcontrib>Thompson, Adrian</creatorcontrib><creatorcontrib>Greenwood, Colin</creatorcontrib><creatorcontrib>Hajdu, Janos</creatorcontrib><title>Crystallization and Preliminary X-ray Analysis of the Di-Haem Cytochrome c Peroxidase from Pseudomonas aeruginosa</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>Cytochrome
c
551 peroxidase is a periplasmic enzyme expressed in
Pseudomas aeruginosa at low oxygen tensions. The glycosylated enzyme has been purified to homogeneity and crystallized by vapour diffusion techniques using polyethylene glycol 2000 as the precipitant in the presence of isopropanol. The crystals belong to the trigonal space group
P 3
121 or
P3
221 with unit cell dimensions of
a =
b = 113·8 Å,
c = 72·0 Å. They are suitable for X-ray analysis and diffract to
d
min = 2·5 Å. There is one peroxidase molecule in the crystallographic asymmetric unit.</description><subject>Biological and medical sciences</subject><subject>Crystalline structure</subject><subject>crystallization</subject><subject>cytochrome c551 peroxidase</subject><subject>Cytochrome-c Peroxidase - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoproteins - chemistry</subject><subject>glycosylation</subject><subject>Hemeproteins - chemistry</subject><subject>Molecular biophysics</subject><subject>periplasmic protein</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - enzymology</subject><subject>Structure in molecular biology</subject><subject>X-Ray Diffraction</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EKkvhyg3JB8Qti7_i2MdqoRSpEnsAiZs1dibUVRK3dlKR_vVk2VVviNNI837zNHqPkLecbTlj-uPt4OOWWyu3XDXiGdlwZmxltDTPyYYxISphpH5JXpVyyxirpTJn5MxIq7WyG3K_y0uZoO_jI0wxjRTGlu4z9nGII-SF_qwyLPRihH4psdDU0ekG6adYXQEOdLdMKdzkNCANdI85_Y4tFKTduqL7gnObhjRCoYB5_hXHVOA1edFBX_DNaZ6TH5efv--uqutvX77uLq6rILmaKm68NLJrJRMKbGBB1ND62iq0UoEOjW-86KxGrY0R1gNvOm-UEMhQgvXynHw4-t7ldD9jmdwQS8C-hxHTXFxT27oxNf8vyNekRPMX3B7BkFMpGTt3l-OwhuQ4c4cy3KEMdyjDHcpYD96dnGc_YPuEn9Jf9fcnHUqAvsswhlieMGWMaaxcMXPEcI3rIWJ2JUQcA7YxY5hcm-K_PvgDpAGmqg</recordid><startdate>19930820</startdate><enddate>19930820</enddate><creator>Fülöp, Vilmos</creator><creator>Little, Richard</creator><creator>Thompson, Adrian</creator><creator>Greenwood, Colin</creator><creator>Hajdu, Janos</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19930820</creationdate><title>Crystallization and Preliminary X-ray Analysis of the Di-Haem Cytochrome c Peroxidase from Pseudomonas aeruginosa</title><author>Fülöp, Vilmos ; Little, Richard ; Thompson, Adrian ; Greenwood, Colin ; Hajdu, Janos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c314t-18b383fd3024a9c0c25adb594e934a6c7b7b2f96e668829ba17fb8422e0e3a9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Biological and medical sciences</topic><topic>Crystalline structure</topic><topic>crystallization</topic><topic>cytochrome c551 peroxidase</topic><topic>Cytochrome-c Peroxidase - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoproteins - chemistry</topic><topic>glycosylation</topic><topic>Hemeproteins - chemistry</topic><topic>Molecular biophysics</topic><topic>periplasmic protein</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - enzymology</topic><topic>Structure in molecular biology</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fülöp, Vilmos</creatorcontrib><creatorcontrib>Little, Richard</creatorcontrib><creatorcontrib>Thompson, Adrian</creatorcontrib><creatorcontrib>Greenwood, Colin</creatorcontrib><creatorcontrib>Hajdu, Janos</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fülöp, Vilmos</au><au>Little, Richard</au><au>Thompson, Adrian</au><au>Greenwood, Colin</au><au>Hajdu, Janos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Crystallization and Preliminary X-ray Analysis of the Di-Haem Cytochrome c Peroxidase from Pseudomonas aeruginosa</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>1993-08-20</date><risdate>1993</risdate><volume>232</volume><issue>4</issue><spage>1208</spage><epage>1210</epage><pages>1208-1210</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><coden>JMOBAK</coden><abstract>Cytochrome
c
551 peroxidase is a periplasmic enzyme expressed in
Pseudomas aeruginosa at low oxygen tensions. The glycosylated enzyme has been purified to homogeneity and crystallized by vapour diffusion techniques using polyethylene glycol 2000 as the precipitant in the presence of isopropanol. The crystals belong to the trigonal space group
P 3
121 or
P3
221 with unit cell dimensions of
a =
b = 113·8 Å,
c = 72·0 Å. They are suitable for X-ray analysis and diffract to
d
min = 2·5 Å. There is one peroxidase molecule in the crystallographic asymmetric unit.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>8396649</pmid><doi>10.1006/jmbi.1993.1472</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2836 |
| ispartof | Journal of molecular biology, 1993-08, Vol.232 (4), p.1208-1210 |
| issn | 0022-2836 1089-8638 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75957851 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Crystalline structure crystallization cytochrome c551 peroxidase Cytochrome-c Peroxidase - chemistry Fundamental and applied biological sciences. Psychology Glycoproteins - chemistry glycosylation Hemeproteins - chemistry Molecular biophysics periplasmic protein Pseudomonas aeruginosa Pseudomonas aeruginosa - enzymology Structure in molecular biology X-Ray Diffraction |
| title | Crystallization and Preliminary X-ray Analysis of the Di-Haem Cytochrome c Peroxidase from Pseudomonas aeruginosa |
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