An empirical assessment of stereological formulae applied to the counting of synaptic disks in the cerebral cortex
Known quantities of test objects approximating the parameters of cortical synapses were embedded in known volumes of a transparent embedding medium. The material was cut in slabs of appropriate thickness. The mean trace length (d) of the profiles of the test objects was measured and the number of pr...
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| Veröffentlicht in: | Journal of comparative neurology (1911) 1985-01, Vol.231 (2), p.175-179 |
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| container_end_page | 179 |
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| container_title | Journal of comparative neurology (1911) |
| container_volume | 231 |
| creator | Colonnier, Marc Beaulieu, Clermont |
| description | Known quantities of test objects approximating the parameters of cortical synapses were embedded in known volumes of a transparent embedding medium. The material was cut in slabs of appropriate thickness. The mean trace length (d) of the profiles of the test objects was measured and the number of profiles per unit area (NA) was calculated. Various stereological formulae were applied to these data to determine the number of test objects per unit volume (NV). For large numbers of those test objects most closely approximating the parameters of cortical synapses, the formula NV = NA/d and the DeHoff and Rhines formula ('61) for polydispersed circular disks NV = 8NAZ/π2 (where Z is the mean of the reciprocals of the trace lengths) gave accurate results (error ≤ 5%). Other popular formulae and procedures were not as accurate and underestimated their number by as much as 32%. |
| doi_str_mv | 10.1002/cne.902310205 |
| format | Article |
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The material was cut in slabs of appropriate thickness. The mean trace length (d) of the profiles of the test objects was measured and the number of profiles per unit area (NA) was calculated. Various stereological formulae were applied to these data to determine the number of test objects per unit volume (NV). For large numbers of those test objects most closely approximating the parameters of cortical synapses, the formula NV = NA/d and the DeHoff and Rhines formula ('61) for polydispersed circular disks NV = 8NAZ/π2 (where Z is the mean of the reciprocals of the trace lengths) gave accurate results (error ≤ 5%). 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Comp. Neurol</addtitle><description>Known quantities of test objects approximating the parameters of cortical synapses were embedded in known volumes of a transparent embedding medium. The material was cut in slabs of appropriate thickness. The mean trace length (d) of the profiles of the test objects was measured and the number of profiles per unit area (NA) was calculated. Various stereological formulae were applied to these data to determine the number of test objects per unit volume (NV). For large numbers of those test objects most closely approximating the parameters of cortical synapses, the formula NV = NA/d and the DeHoff and Rhines formula ('61) for polydispersed circular disks NV = 8NAZ/π2 (where Z is the mean of the reciprocals of the trace lengths) gave accurate results (error ≤ 5%). 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Psychology</subject><subject>Histological Techniques</subject><subject>Mathematics</subject><subject>Models, Neurological</subject><subject>Neuroanatomy - methods</subject><subject>quantitation</subject><subject>stereology</subject><subject>synapse</subject><subject>Synapses</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0021-9967</issn><issn>1096-9861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS1EVZbCkSOSD4hbih3HX8dqVVrQqr20qsTF8jqTYprYwU7U7n-P241WnODkw_vN88x7CH2g5JQSUn9xAU41qRklNeGv0IoSLSqtBH2NVkWnldZCvkFvc_5FCNGaqWN0zJSijeIrlM4ChmH0yTvbY5sz5DxAmHDscJ4gQezj_YvWxTTMvQVsx7H30OIp4uknYBfnMPlw_zKxC3acvMOtzw8Z-7Anis02FQsX0wRP79BRZ_sM75f3BN1-Pb9ZX1ab64tv67NN5VijeaUbxpgSvLHEEdpqoSi1WmhJobNSyC3lbTlNtJxyW6tWSNYIrYiTW-BWcHaCPu99xxR_z5AnM_jsoO9tgDhnI7nmjVD_B2lDai71M1jtQZdizgk6MyY_2LQzlJjnMkwpwxzKKPzHxXjeDtAe6CX9on9adJtLxF2ywfl8wIqRErUqmNxjj76H3b__NOur878XWBb2pcunw6RND6bEJbm5u7ow39d3m0vJNuYH-wNZ0bD_</recordid><startdate>19850108</startdate><enddate>19850108</enddate><creator>Colonnier, Marc</creator><creator>Beaulieu, Clermont</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19850108</creationdate><title>An empirical assessment of stereological formulae applied to the counting of synaptic disks in the cerebral cortex</title><author>Colonnier, Marc ; Beaulieu, Clermont</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3495-943338654a0c01d96811a96971efa767b15d9966d515a28d67346980c7be5a653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Anatomy</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cats</topic><topic>Central nervous system</topic><topic>Cerebral Cortex - anatomy & histology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Histological Techniques</topic><topic>Mathematics</topic><topic>Models, Neurological</topic><topic>Neuroanatomy - methods</topic><topic>quantitation</topic><topic>stereology</topic><topic>synapse</topic><topic>Synapses</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Colonnier, Marc</creatorcontrib><creatorcontrib>Beaulieu, Clermont</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of comparative neurology (1911)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Colonnier, Marc</au><au>Beaulieu, Clermont</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An empirical assessment of stereological formulae applied to the counting of synaptic disks in the cerebral cortex</atitle><jtitle>Journal of comparative neurology (1911)</jtitle><addtitle>J. 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| ispartof | Journal of comparative neurology (1911), 1985-01, Vol.231 (2), p.175-179 |
| issn | 0021-9967 1096-9861 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Anatomy Animals Biological and medical sciences Cats Central nervous system Cerebral Cortex - anatomy & histology Fundamental and applied biological sciences. Psychology Histological Techniques Mathematics Models, Neurological Neuroanatomy - methods quantitation stereology synapse Synapses Vertebrates: nervous system and sense organs |
| title | An empirical assessment of stereological formulae applied to the counting of synaptic disks in the cerebral cortex |
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