Role of the pepper cytochrome P450 gene CaCYP450A in defense responses against microbial pathogens
Plant cytochrome P450 enzymes are involved in a wide range of biosynthetic reactions, leading to various fatty acid conjugates, plant hormones, or defensive compounds. Herein, we have identified the pepper cytochrome P450 gene CaCYP450A, which is differentially induced during Xanthomonas campestris...
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| Veröffentlicht in: | Planta 2010-11, Vol.232 (6), p.1409-1421 |
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| description | Plant cytochrome P450 enzymes are involved in a wide range of biosynthetic reactions, leading to various fatty acid conjugates, plant hormones, or defensive compounds. Herein, we have identified the pepper cytochrome P450 gene CaCYP450A, which is differentially induced during Xanthomonas campestris pv. vesicatoria (Xcv) infection. CaCYP450A contains a heme-binding motif, PXFXXGXRXCXG, located in the C-terminal region and a hydrophobic membrane anchor region at the N terminal. Knock-down of CaCYP450A by virus-induced gene silencing (VIGS) led to increased susceptibility to Xcv infection in pepper. CaCYP450A-overexpressing Arabidopsis plants exhibited lower pathogen growth and reduced disease symptoms, and they were more resistant to Pseudomonas syringae pv. tomato (Pst) and Hyaloperonospora arabidopsidis than wild-type plants. Overexpression of CaCYP450A also enhanced H₂O₂ accumulation and cell death. However, CaCYP450A Arabidopsis ortholog CYP94B3 mutants showed enhanced susceptibility to virulent Pst DC3000, but not to avirulent Pst DC3000 avrRpm1 or virulent H. arabidopsidis infection. Taken together, these results suggest that CaCYP450A is required for defense responses to microbial pathogens in plants. The nucleotide sequence data reported here has been deposited in the GenBank database under the accession number HM581974. |
| doi_str_mv | 10.1007/s00425-010-1266-y |
| format | Article |
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Herein, we have identified the pepper cytochrome P450 gene CaCYP450A, which is differentially induced during Xanthomonas campestris pv. vesicatoria (Xcv) infection. CaCYP450A contains a heme-binding motif, PXFXXGXRXCXG, located in the C-terminal region and a hydrophobic membrane anchor region at the N terminal. Knock-down of CaCYP450A by virus-induced gene silencing (VIGS) led to increased susceptibility to Xcv infection in pepper. CaCYP450A-overexpressing Arabidopsis plants exhibited lower pathogen growth and reduced disease symptoms, and they were more resistant to Pseudomonas syringae pv. tomato (Pst) and Hyaloperonospora arabidopsidis than wild-type plants. Overexpression of CaCYP450A also enhanced H₂O₂ accumulation and cell death. However, CaCYP450A Arabidopsis ortholog CYP94B3 mutants showed enhanced susceptibility to virulent Pst DC3000, but not to avirulent Pst DC3000 avrRpm1 or virulent H. arabidopsidis infection. Taken together, these results suggest that CaCYP450A is required for defense responses to microbial pathogens in plants. The nucleotide sequence data reported here has been deposited in the GenBank database under the accession number HM581974.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s00425-010-1266-y</identifier><identifier>PMID: 20830594</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Agriculture ; Amino Acid Sequence ; Biological and medical sciences ; Biomedical and Life Sciences ; Capsicum - enzymology ; Capsicum - genetics ; Capsicum - microbiology ; Capsicum annuum ; cell death ; Cytochrome ; cytochrome P-450 ; Cytochrome P-450 Enzyme System - chemistry ; Cytochrome P-450 Enzyme System - genetics ; Cytochromes ; Defense responses ; Ecology ; Forestry ; Fundamental and applied biological sciences. Psychology ; Gene Knockdown Techniques ; Genes, Plant ; Hormones ; Infections ; Inoculation ; Leaves ; Life Sciences ; Molecular Sequence Data ; Original Article ; Pathogens ; Peppers ; Plant growth ; Plant Sciences ; Plants ; Plants, Genetically Modified ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Amino Acid ; Tomatoes ; Transgenic Arabidopsis ; Transgenic plants ; VIGS assay ; Xanthomonas campestris ; Xanthomonas campestris - pathogenicity</subject><ispartof>Planta, 2010-11, Vol.232 (6), p.1409-1421</ispartof><rights>Springer-Verlag Berlin Heidelberg 2010</rights><rights>Springer-Verlag 2010</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-6135c97dced8c9e0f8e069979fe6833915fff63ac810a82ebe4a2d1d0cfa533b3</citedby><cites>FETCH-LOGICAL-c512t-6135c97dced8c9e0f8e069979fe6833915fff63ac810a82ebe4a2d1d0cfa533b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23391906$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23391906$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27903,27904,41467,42536,51297,57995,58228</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23419848$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20830594$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hwang, In Sun</creatorcontrib><creatorcontrib>Hwang, Byung Kook</creatorcontrib><title>Role of the pepper cytochrome P450 gene CaCYP450A in defense responses against microbial pathogens</title><title>Planta</title><addtitle>Planta</addtitle><addtitle>Planta</addtitle><description>Plant cytochrome P450 enzymes are involved in a wide range of biosynthetic reactions, leading to various fatty acid conjugates, plant hormones, or defensive compounds. Herein, we have identified the pepper cytochrome P450 gene CaCYP450A, which is differentially induced during Xanthomonas campestris pv. vesicatoria (Xcv) infection. CaCYP450A contains a heme-binding motif, PXFXXGXRXCXG, located in the C-terminal region and a hydrophobic membrane anchor region at the N terminal. Knock-down of CaCYP450A by virus-induced gene silencing (VIGS) led to increased susceptibility to Xcv infection in pepper. CaCYP450A-overexpressing Arabidopsis plants exhibited lower pathogen growth and reduced disease symptoms, and they were more resistant to Pseudomonas syringae pv. tomato (Pst) and Hyaloperonospora arabidopsidis than wild-type plants. Overexpression of CaCYP450A also enhanced H₂O₂ accumulation and cell death. However, CaCYP450A Arabidopsis ortholog CYP94B3 mutants showed enhanced susceptibility to virulent Pst DC3000, but not to avirulent Pst DC3000 avrRpm1 or virulent H. arabidopsidis infection. Taken together, these results suggest that CaCYP450A is required for defense responses to microbial pathogens in plants. The nucleotide sequence data reported here has been deposited in the GenBank database under the accession number HM581974.</description><subject>Agriculture</subject><subject>Amino Acid Sequence</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Capsicum - enzymology</subject><subject>Capsicum - genetics</subject><subject>Capsicum - microbiology</subject><subject>Capsicum annuum</subject><subject>cell death</subject><subject>Cytochrome</subject><subject>cytochrome P-450</subject><subject>Cytochrome P-450 Enzyme System - chemistry</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Cytochromes</subject><subject>Defense responses</subject><subject>Ecology</subject><subject>Forestry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Knockdown Techniques</subject><subject>Genes, Plant</subject><subject>Hormones</subject><subject>Infections</subject><subject>Inoculation</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Molecular Sequence Data</subject><subject>Original Article</subject><subject>Pathogens</subject><subject>Peppers</subject><subject>Plant growth</subject><subject>Plant Sciences</subject><subject>Plants</subject><subject>Plants, Genetically Modified</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Sequence Homology, Amino Acid</subject><subject>Tomatoes</subject><subject>Transgenic Arabidopsis</subject><subject>Transgenic plants</subject><subject>VIGS assay</subject><subject>Xanthomonas campestris</subject><subject>Xanthomonas campestris - pathogenicity</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kEFv1DAQhS0EokvhB3AALCTEKTC248Q-VitakCq1AnrgZDnOeDerJA529rD_vl5loYhDT57R--bN-BHymsEnBlB_TgAllwUwKBivquLwhKxYKXjBoVRPyQog16CFPCMvUtoBZLGun5MzDkqA1OWKNN9DjzR4Om-RTjhNGKk7zMFtYxiQ3pYS6AZHpGu7_nXsLmg30hY9jglpxDSFXCRqN7Yb00yHzsXQdLank523IY-ml-SZt33CV6f3nNxdfvm5_lpc31x9W19cF04yPhcVE9LpunXYKqcRvEKotK61x0oJoZn03lfCOsXAKo4Nlpa3rAXnrRSiEefk4-I7xfB7j2k2Q5cc9r0dMeyTqaWWnGfLTL7_j9yFfRzzcUaBVLWUgmWILVD-UEoRvZliN9h4MAzMMX6zxG_g2Of4zSHPvD0Z75sB278Tf_LOwIcTYJOzvY92dF164ETJtCpV5vjCpSyNG4wPFz62_c0ytEtziP-Y5vA0VFl_t-jeBmM3MS---8GBCcgyzybiHin_rsc</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Hwang, In Sun</creator><creator>Hwang, Byung Kook</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20101101</creationdate><title>Role of the pepper cytochrome P450 gene CaCYP450A in defense responses against microbial pathogens</title><author>Hwang, In Sun ; Hwang, Byung Kook</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-6135c97dced8c9e0f8e069979fe6833915fff63ac810a82ebe4a2d1d0cfa533b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Agriculture</topic><topic>Amino Acid Sequence</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Capsicum - enzymology</topic><topic>Capsicum - genetics</topic><topic>Capsicum - microbiology</topic><topic>Capsicum annuum</topic><topic>cell death</topic><topic>Cytochrome</topic><topic>cytochrome P-450</topic><topic>Cytochrome P-450 Enzyme System - chemistry</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>Cytochromes</topic><topic>Defense responses</topic><topic>Ecology</topic><topic>Forestry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Knockdown Techniques</topic><topic>Genes, Plant</topic><topic>Hormones</topic><topic>Infections</topic><topic>Inoculation</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Molecular Sequence Data</topic><topic>Original Article</topic><topic>Pathogens</topic><topic>Peppers</topic><topic>Plant growth</topic><topic>Plant Sciences</topic><topic>Plants</topic><topic>Plants, Genetically Modified</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Sequence Homology, Amino Acid</topic><topic>Tomatoes</topic><topic>Transgenic Arabidopsis</topic><topic>Transgenic plants</topic><topic>VIGS assay</topic><topic>Xanthomonas campestris</topic><topic>Xanthomonas campestris - pathogenicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hwang, In Sun</creatorcontrib><creatorcontrib>Hwang, Byung Kook</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hwang, In Sun</au><au>Hwang, Byung Kook</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of the pepper cytochrome P450 gene CaCYP450A in defense responses against microbial pathogens</atitle><jtitle>Planta</jtitle><stitle>Planta</stitle><addtitle>Planta</addtitle><date>2010-11-01</date><risdate>2010</risdate><volume>232</volume><issue>6</issue><spage>1409</spage><epage>1421</epage><pages>1409-1421</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>Plant cytochrome P450 enzymes are involved in a wide range of biosynthetic reactions, leading to various fatty acid conjugates, plant hormones, or defensive compounds. Herein, we have identified the pepper cytochrome P450 gene CaCYP450A, which is differentially induced during Xanthomonas campestris pv. vesicatoria (Xcv) infection. CaCYP450A contains a heme-binding motif, PXFXXGXRXCXG, located in the C-terminal region and a hydrophobic membrane anchor region at the N terminal. Knock-down of CaCYP450A by virus-induced gene silencing (VIGS) led to increased susceptibility to Xcv infection in pepper. CaCYP450A-overexpressing Arabidopsis plants exhibited lower pathogen growth and reduced disease symptoms, and they were more resistant to Pseudomonas syringae pv. tomato (Pst) and Hyaloperonospora arabidopsidis than wild-type plants. Overexpression of CaCYP450A also enhanced H₂O₂ accumulation and cell death. However, CaCYP450A Arabidopsis ortholog CYP94B3 mutants showed enhanced susceptibility to virulent Pst DC3000, but not to avirulent Pst DC3000 avrRpm1 or virulent H. arabidopsidis infection. Taken together, these results suggest that CaCYP450A is required for defense responses to microbial pathogens in plants. The nucleotide sequence data reported here has been deposited in the GenBank database under the accession number HM581974.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>20830594</pmid><doi>10.1007/s00425-010-1266-y</doi><tpages>13</tpages></addata></record> |
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| subjects | Agriculture Amino Acid Sequence Biological and medical sciences Biomedical and Life Sciences Capsicum - enzymology Capsicum - genetics Capsicum - microbiology Capsicum annuum cell death Cytochrome cytochrome P-450 Cytochrome P-450 Enzyme System - chemistry Cytochrome P-450 Enzyme System - genetics Cytochromes Defense responses Ecology Forestry Fundamental and applied biological sciences. Psychology Gene Knockdown Techniques Genes, Plant Hormones Infections Inoculation Leaves Life Sciences Molecular Sequence Data Original Article Pathogens Peppers Plant growth Plant Sciences Plants Plants, Genetically Modified Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Tomatoes Transgenic Arabidopsis Transgenic plants VIGS assay Xanthomonas campestris Xanthomonas campestris - pathogenicity |
| title | Role of the pepper cytochrome P450 gene CaCYP450A in defense responses against microbial pathogens |
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