Proinflammatory Cytokines Enhance Human Synoviocyte Expression of Functional Intercellular Adhesion Molecule-1 (ICAM-1)
We determined the ability of proinflammatory cytokines to enhance ICAM-1 (CD54) expression on, and PBMC adhesion to, human synoviocytes. Surface molecules were characterized by cell ELISA and by flow cytometry. Adhesion of PBMC to synoviocyte monolayers was measured by direct counting or by colorime...
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| Veröffentlicht in: | Clinical immunology and immunopathology 1993-09, Vol.68 (3), p.311-320 |
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| container_title | Clinical immunology and immunopathology |
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| creator | Lindsley, Herbert B. Smith, Donald D. Cohick, Christopher B. Koch, Alisa E. Davis, Laurie S. |
| description | We determined the ability of proinflammatory cytokines to enhance ICAM-1 (CD54) expression on, and PBMC adhesion to, human synoviocytes. Surface molecules were characterized by cell ELISA and by flow cytometry. Adhesion of PBMC to synoviocyte monolayers was measured by direct counting or by colorimetric staining. Most cytokines upregulated ICAM-1 expression (IL-1β > TNFα > IFN-γ ⪢ PDGF-bb, IL-6), but not GM-CSF or TGFβ. A similar concentration-dependent increase was observed for synoviocytes derived from patients with rheumatoid or osteoarthritis. Kinetic studies of ICAM-1 expression differed among several cytokines: an early rise with IL-1β or TNFα stimulation, a gradual increase with IFN-γ, a transient increase with PDGF-bb, and a plateau with IL-6. Adhesion of PBMC to synoviocytes was increased by IL-1β or TNFα and reduced by MAb to CD54 or CD18. Increased synoviocyte adhesiveness may promote interactions with infiltrating inflammatory cells. |
| doi_str_mv | 10.1006/clin.1993.1132 |
| format | Article |
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Surface molecules were characterized by cell ELISA and by flow cytometry. Adhesion of PBMC to synoviocyte monolayers was measured by direct counting or by colorimetric staining. Most cytokines upregulated ICAM-1 expression (IL-1β > TNFα > IFN-γ ⪢ PDGF-bb, IL-6), but not GM-CSF or TGFβ. A similar concentration-dependent increase was observed for synoviocytes derived from patients with rheumatoid or osteoarthritis. Kinetic studies of ICAM-1 expression differed among several cytokines: an early rise with IL-1β or TNFα stimulation, a gradual increase with IFN-γ, a transient increase with PDGF-bb, and a plateau with IL-6. Adhesion of PBMC to synoviocytes was increased by IL-1β or TNFα and reduced by MAb to CD54 or CD18. Increased synoviocyte adhesiveness may promote interactions with infiltrating inflammatory cells.</description><identifier>ISSN: 0090-1229</identifier><identifier>EISSN: 1090-2341</identifier><identifier>DOI: 10.1006/clin.1993.1132</identifier><identifier>PMID: 8103722</identifier><identifier>CODEN: CLIIAT</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Arthritis, Rheumatoid - metabolism ; Arthritis, Rheumatoid - pathology ; Biological and medical sciences ; Cell Adhesion ; Cell Adhesion Molecules - metabolism ; Cytokines - pharmacology ; Diseases of the osteoarticular system ; Humans ; Inflammatory joint diseases ; Intercellular Adhesion Molecule-1 ; Medical sciences ; Osteoarthritis - metabolism ; Osteoarthritis - pathology ; Synovial Membrane - metabolism</subject><ispartof>Clinical immunology and immunopathology, 1993-09, Vol.68 (3), p.311-320</ispartof><rights>1993 Academic Press</rights><rights>1994 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-fdd0547ff98d14e305ea2e3f579fc40923f9652321db35e7a32a19f9823c52963</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3778990$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8103722$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lindsley, Herbert B.</creatorcontrib><creatorcontrib>Smith, Donald D.</creatorcontrib><creatorcontrib>Cohick, Christopher B.</creatorcontrib><creatorcontrib>Koch, Alisa E.</creatorcontrib><creatorcontrib>Davis, Laurie S.</creatorcontrib><title>Proinflammatory Cytokines Enhance Human Synoviocyte Expression of Functional Intercellular Adhesion Molecule-1 (ICAM-1)</title><title>Clinical immunology and immunopathology</title><addtitle>Clin Immunol Immunopathol</addtitle><description>We determined the ability of proinflammatory cytokines to enhance ICAM-1 (CD54) expression on, and PBMC adhesion to, human synoviocytes. Surface molecules were characterized by cell ELISA and by flow cytometry. Adhesion of PBMC to synoviocyte monolayers was measured by direct counting or by colorimetric staining. Most cytokines upregulated ICAM-1 expression (IL-1β > TNFα > IFN-γ ⪢ PDGF-bb, IL-6), but not GM-CSF or TGFβ. A similar concentration-dependent increase was observed for synoviocytes derived from patients with rheumatoid or osteoarthritis. Kinetic studies of ICAM-1 expression differed among several cytokines: an early rise with IL-1β or TNFα stimulation, a gradual increase with IFN-γ, a transient increase with PDGF-bb, and a plateau with IL-6. Adhesion of PBMC to synoviocytes was increased by IL-1β or TNFα and reduced by MAb to CD54 or CD18. Increased synoviocyte adhesiveness may promote interactions with infiltrating inflammatory cells.</description><subject>Arthritis, Rheumatoid - metabolism</subject><subject>Arthritis, Rheumatoid - pathology</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>Cytokines - pharmacology</subject><subject>Diseases of the osteoarticular system</subject><subject>Humans</subject><subject>Inflammatory joint diseases</subject><subject>Intercellular Adhesion Molecule-1</subject><subject>Medical sciences</subject><subject>Osteoarthritis - metabolism</subject><subject>Osteoarthritis - pathology</subject><subject>Synovial Membrane - metabolism</subject><issn>0090-1229</issn><issn>1090-2341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1P3DAQhq2qCLa0V25IPlSIHrL1R7KOj2i1FCQQlQpnyzhjYerYWzuh5N_jdFfcepqR5plXrx6ETihZUkJW3413YUml5EtKOfuAFpRIUjFe049oQeadMiaP0Kecn0l5qIk4RIctJVwwtkB_f6bogvW67_UQ04TX0xB_uwAZb8KTDgbw1djrgH9NIb64aKYB8OZ1myBnFwOOFl-OwQxl1x5fhwGSAe9HrxO-6J7gH3QbPZjRQ0Xx-fX64rai3z6jA6t9hi_7eYweLjf366vq5u5HQW4qw1ftUNmuI00trJVtR2vgpAHNgNtGSGtqIhm3ctUwzmj3yBsQmjNNZaEZNw2TK36Mzna52xT_jJAH1bs8N9QB4piVaGQtaEsLuNyBJsWcE1i1Ta7XaVKUqNm0mk2r2bSaTZeH033y-NhD947v1Zb71_1dZ6O9TUWmy-8YF6KVkhSs3WFQLLw4SCobB8V75xKYQXXR_a_BG3l-mgk</recordid><startdate>19930901</startdate><enddate>19930901</enddate><creator>Lindsley, Herbert B.</creator><creator>Smith, Donald D.</creator><creator>Cohick, Christopher B.</creator><creator>Koch, Alisa E.</creator><creator>Davis, Laurie S.</creator><general>Elsevier Inc</general><general>Academic Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930901</creationdate><title>Proinflammatory Cytokines Enhance Human Synoviocyte Expression of Functional Intercellular Adhesion Molecule-1 (ICAM-1)</title><author>Lindsley, Herbert B. ; Smith, Donald D. ; Cohick, Christopher B. ; Koch, Alisa E. ; Davis, Laurie S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-fdd0547ff98d14e305ea2e3f579fc40923f9652321db35e7a32a19f9823c52963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Arthritis, Rheumatoid - metabolism</topic><topic>Arthritis, Rheumatoid - pathology</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>Cytokines - pharmacology</topic><topic>Diseases of the osteoarticular system</topic><topic>Humans</topic><topic>Inflammatory joint diseases</topic><topic>Intercellular Adhesion Molecule-1</topic><topic>Medical sciences</topic><topic>Osteoarthritis - metabolism</topic><topic>Osteoarthritis - pathology</topic><topic>Synovial Membrane - metabolism</topic><toplevel>online_resources</toplevel><creatorcontrib>Lindsley, Herbert B.</creatorcontrib><creatorcontrib>Smith, Donald D.</creatorcontrib><creatorcontrib>Cohick, Christopher B.</creatorcontrib><creatorcontrib>Koch, Alisa E.</creatorcontrib><creatorcontrib>Davis, Laurie S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lindsley, Herbert B.</au><au>Smith, Donald D.</au><au>Cohick, Christopher B.</au><au>Koch, Alisa E.</au><au>Davis, Laurie S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proinflammatory Cytokines Enhance Human Synoviocyte Expression of Functional Intercellular Adhesion Molecule-1 (ICAM-1)</atitle><jtitle>Clinical immunology and immunopathology</jtitle><addtitle>Clin Immunol Immunopathol</addtitle><date>1993-09-01</date><risdate>1993</risdate><volume>68</volume><issue>3</issue><spage>311</spage><epage>320</epage><pages>311-320</pages><issn>0090-1229</issn><eissn>1090-2341</eissn><coden>CLIIAT</coden><abstract>We determined the ability of proinflammatory cytokines to enhance ICAM-1 (CD54) expression on, and PBMC adhesion to, human synoviocytes. Surface molecules were characterized by cell ELISA and by flow cytometry. Adhesion of PBMC to synoviocyte monolayers was measured by direct counting or by colorimetric staining. Most cytokines upregulated ICAM-1 expression (IL-1β > TNFα > IFN-γ ⪢ PDGF-bb, IL-6), but not GM-CSF or TGFβ. A similar concentration-dependent increase was observed for synoviocytes derived from patients with rheumatoid or osteoarthritis. Kinetic studies of ICAM-1 expression differed among several cytokines: an early rise with IL-1β or TNFα stimulation, a gradual increase with IFN-γ, a transient increase with PDGF-bb, and a plateau with IL-6. Adhesion of PBMC to synoviocytes was increased by IL-1β or TNFα and reduced by MAb to CD54 or CD18. Increased synoviocyte adhesiveness may promote interactions with infiltrating inflammatory cells.</abstract><cop>San Diego, CA</cop><cop>New York, NY</cop><cop>Boston</cop><pub>Elsevier Inc</pub><pmid>8103722</pmid><doi>10.1006/clin.1993.1132</doi><tpages>10</tpages></addata></record> |
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| subjects | Arthritis, Rheumatoid - metabolism Arthritis, Rheumatoid - pathology Biological and medical sciences Cell Adhesion Cell Adhesion Molecules - metabolism Cytokines - pharmacology Diseases of the osteoarticular system Humans Inflammatory joint diseases Intercellular Adhesion Molecule-1 Medical sciences Osteoarthritis - metabolism Osteoarthritis - pathology Synovial Membrane - metabolism |
| title | Proinflammatory Cytokines Enhance Human Synoviocyte Expression of Functional Intercellular Adhesion Molecule-1 (ICAM-1) |
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