Diagnostic compatibility of a thymidine kinase, inverted repeat, gI, and gpX modified live gene-deleted PRV vaccine with three differential ELISAs
Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State University, Ames 50011. The differential pseudorabies virus (PRV) vaccines currently in use in the USA have deletions of the genes coding for the glycoprotein I (gI) and/or glycoprotein X (gpX). The absence of gI and/or gpX...
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| Veröffentlicht in: | Journal of veterinary diagnostic investigation 1993-07, Vol.5 (3), p.347-350 |
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| container_title | Journal of veterinary diagnostic investigation |
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| creator | Swenson, SL McMillen, J Hill, HT |
| description | Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State University, Ames 50011.
The differential pseudorabies virus (PRV) vaccines currently in use in the USA have deletions of the genes coding for the glycoprotein I (gI) and/or glycoprotein X (gpX). The absence of gI and/or gpX allow for the serologic differentiation of vaccinated swine from PRV-infected swine using differential enzyme-linked immunosorbent assays (ELISAs). A newly developed pseudorabies vaccine virus has 4 deletions of the viral genome: the genes coding for gI, gpX, and thymidine kinase and a portion of the repeat region to attenuate the virus. The purpose of this work was to evaluate the diagnostic compatibility of the gI/gpX gene-deleted vaccine with 3 differential vaccines and 3 differential ELISAs currently in use. Pigs vaccinated 3 times with the gI/gpX gene-deleted vaccine remained seronegative on the 3 differential ELISAs tested. Pigs previously vaccinated with either a gI or gpX gene-deleted vaccine and then vaccinated with the gI/gpX gene-deleted vaccine remained seronegative on the respective gI or gpX differential assay. |
| doi_str_mv | 10.1177/104063879300500307 |
| format | Article |
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The differential pseudorabies virus (PRV) vaccines currently in use in the USA have deletions of the genes coding for the glycoprotein I (gI) and/or glycoprotein X (gpX). The absence of gI and/or gpX allow for the serologic differentiation of vaccinated swine from PRV-infected swine using differential enzyme-linked immunosorbent assays (ELISAs). A newly developed pseudorabies vaccine virus has 4 deletions of the viral genome: the genes coding for gI, gpX, and thymidine kinase and a portion of the repeat region to attenuate the virus. The purpose of this work was to evaluate the diagnostic compatibility of the gI/gpX gene-deleted vaccine with 3 differential vaccines and 3 differential ELISAs currently in use. Pigs vaccinated 3 times with the gI/gpX gene-deleted vaccine remained seronegative on the 3 differential ELISAs tested. Pigs previously vaccinated with either a gI or gpX gene-deleted vaccine and then vaccinated with the gI/gpX gene-deleted vaccine remained seronegative on the respective gI or gpX differential assay.</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/104063879300500307</identifier><identifier>PMID: 8396984</identifier><language>eng</language><publisher>Los Angeles, CA: J Vet Diagn Invest</publisher><subject>Animals ; Antibodies, Viral - blood ; Chromosome Inversion ; Enzyme-Linked Immunosorbent Assay - veterinary ; Gene Deletion ; Genes, Viral - genetics ; Herpesvirus 1, Suid - enzymology ; Herpesvirus 1, Suid - genetics ; Herpesvirus 1, Suid - immunology ; Pseudorabies - diagnosis ; Pseudorabies - immunology ; Pseudorabies Vaccines ; Repetitive Sequences, Nucleic Acid ; Serologic Tests ; Swine ; Swine Diseases - diagnosis ; Swine Diseases - immunology ; Thymidine Kinase - genetics ; Vaccines, Attenuated - immunology ; Viral Envelope Proteins - genetics</subject><ispartof>Journal of veterinary diagnostic investigation, 1993-07, Vol.5 (3), p.347-350</ispartof><rights>1993 American Association of Veterinary Laboratory Diagnosticians</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c283t-e4a503dcead286d0bdce797c12a230b88641fe713ce6eb61a7f2e7ad80a64efd3</citedby><cites>FETCH-LOGICAL-c283t-e4a503dcead286d0bdce797c12a230b88641fe713ce6eb61a7f2e7ad80a64efd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8396984$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Swenson, SL</creatorcontrib><creatorcontrib>McMillen, J</creatorcontrib><creatorcontrib>Hill, HT</creatorcontrib><title>Diagnostic compatibility of a thymidine kinase, inverted repeat, gI, and gpX modified live gene-deleted PRV vaccine with three differential ELISAs</title><title>Journal of veterinary diagnostic investigation</title><addtitle>J Vet Diagn Invest</addtitle><description>Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State University, Ames 50011.
The differential pseudorabies virus (PRV) vaccines currently in use in the USA have deletions of the genes coding for the glycoprotein I (gI) and/or glycoprotein X (gpX). The absence of gI and/or gpX allow for the serologic differentiation of vaccinated swine from PRV-infected swine using differential enzyme-linked immunosorbent assays (ELISAs). A newly developed pseudorabies vaccine virus has 4 deletions of the viral genome: the genes coding for gI, gpX, and thymidine kinase and a portion of the repeat region to attenuate the virus. The purpose of this work was to evaluate the diagnostic compatibility of the gI/gpX gene-deleted vaccine with 3 differential vaccines and 3 differential ELISAs currently in use. Pigs vaccinated 3 times with the gI/gpX gene-deleted vaccine remained seronegative on the 3 differential ELISAs tested. Pigs previously vaccinated with either a gI or gpX gene-deleted vaccine and then vaccinated with the gI/gpX gene-deleted vaccine remained seronegative on the respective gI or gpX differential assay.</description><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>Chromosome Inversion</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Gene Deletion</subject><subject>Genes, Viral - genetics</subject><subject>Herpesvirus 1, Suid - enzymology</subject><subject>Herpesvirus 1, Suid - genetics</subject><subject>Herpesvirus 1, Suid - immunology</subject><subject>Pseudorabies - diagnosis</subject><subject>Pseudorabies - immunology</subject><subject>Pseudorabies Vaccines</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Serologic Tests</subject><subject>Swine</subject><subject>Swine Diseases - diagnosis</subject><subject>Swine Diseases - immunology</subject><subject>Thymidine Kinase - genetics</subject><subject>Vaccines, Attenuated - immunology</subject><subject>Viral Envelope Proteins - genetics</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2LFDEQhhtR1nX1DwhCLnqadpNOptN9XNZVBwYUv_AWqpPqnhr7a5PMLPM3_MVmmMGL4CkF7_M-kKoseyn4WyG0vhZc8VJWupacLzmXXD_KLkWtZK5qWT5OcwLyI_E0exbCNlHFUouL7KKSdVlX6jL7_Y6gG6cQyTI7DTNEaqineGBTy4DFzWEgRyOyXzRCwAWjcY8-omMeZ4S4YN1qwWB0rJt_smFy1FIKe9oj63DE3GGPR_zzlx9sD9YeXQ8UN0ntEVniW_Q4RoKe3a1XX2_C8-xJC33AF-f3Kvv-_u7b7cd8_enD6vZmnduikjFHBUsunUVwRVU63qRR19qKAgrJm6oqlWhRC2mxxKYUoNsCNbiKQ6mwdfIqe3Pyzn6632GIZqBgse9hxGkXjF7WSmkuElicQOunEDy2ZvY0gD8Ywc3xEObfQ6TSq7N91wzo_lbOm0_59SkP0KHZTjs_ps_-3_j61NhQt3kgjyYM0PfJX5jt3tHSSCOVln8ASRCfqg</recordid><startdate>199307</startdate><enddate>199307</enddate><creator>Swenson, SL</creator><creator>McMillen, J</creator><creator>Hill, HT</creator><general>J Vet Diagn Invest</general><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199307</creationdate><title>Diagnostic compatibility of a thymidine kinase, inverted repeat, gI, and gpX modified live gene-deleted PRV vaccine with three differential ELISAs</title><author>Swenson, SL ; McMillen, J ; Hill, HT</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c283t-e4a503dcead286d0bdce797c12a230b88641fe713ce6eb61a7f2e7ad80a64efd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>Chromosome Inversion</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Gene Deletion</topic><topic>Genes, Viral - genetics</topic><topic>Herpesvirus 1, Suid - enzymology</topic><topic>Herpesvirus 1, Suid - genetics</topic><topic>Herpesvirus 1, Suid - immunology</topic><topic>Pseudorabies - diagnosis</topic><topic>Pseudorabies - immunology</topic><topic>Pseudorabies Vaccines</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Serologic Tests</topic><topic>Swine</topic><topic>Swine Diseases - diagnosis</topic><topic>Swine Diseases - immunology</topic><topic>Thymidine Kinase - genetics</topic><topic>Vaccines, Attenuated - immunology</topic><topic>Viral Envelope Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Swenson, SL</creatorcontrib><creatorcontrib>McMillen, J</creatorcontrib><creatorcontrib>Hill, HT</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Swenson, SL</au><au>McMillen, J</au><au>Hill, HT</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diagnostic compatibility of a thymidine kinase, inverted repeat, gI, and gpX modified live gene-deleted PRV vaccine with three differential ELISAs</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><addtitle>J Vet Diagn Invest</addtitle><date>1993-07</date><risdate>1993</risdate><volume>5</volume><issue>3</issue><spage>347</spage><epage>350</epage><pages>347-350</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><abstract>Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State University, Ames 50011.
The differential pseudorabies virus (PRV) vaccines currently in use in the USA have deletions of the genes coding for the glycoprotein I (gI) and/or glycoprotein X (gpX). The absence of gI and/or gpX allow for the serologic differentiation of vaccinated swine from PRV-infected swine using differential enzyme-linked immunosorbent assays (ELISAs). A newly developed pseudorabies vaccine virus has 4 deletions of the viral genome: the genes coding for gI, gpX, and thymidine kinase and a portion of the repeat region to attenuate the virus. The purpose of this work was to evaluate the diagnostic compatibility of the gI/gpX gene-deleted vaccine with 3 differential vaccines and 3 differential ELISAs currently in use. Pigs vaccinated 3 times with the gI/gpX gene-deleted vaccine remained seronegative on the 3 differential ELISAs tested. Pigs previously vaccinated with either a gI or gpX gene-deleted vaccine and then vaccinated with the gI/gpX gene-deleted vaccine remained seronegative on the respective gI or gpX differential assay.</abstract><cop>Los Angeles, CA</cop><pub>J Vet Diagn Invest</pub><pmid>8396984</pmid><doi>10.1177/104063879300500307</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of veterinary diagnostic investigation, 1993-07, Vol.5 (3), p.347-350 |
| issn | 1040-6387 1943-4936 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Antibodies, Viral - blood Chromosome Inversion Enzyme-Linked Immunosorbent Assay - veterinary Gene Deletion Genes, Viral - genetics Herpesvirus 1, Suid - enzymology Herpesvirus 1, Suid - genetics Herpesvirus 1, Suid - immunology Pseudorabies - diagnosis Pseudorabies - immunology Pseudorabies Vaccines Repetitive Sequences, Nucleic Acid Serologic Tests Swine Swine Diseases - diagnosis Swine Diseases - immunology Thymidine Kinase - genetics Vaccines, Attenuated - immunology Viral Envelope Proteins - genetics |
| title | Diagnostic compatibility of a thymidine kinase, inverted repeat, gI, and gpX modified live gene-deleted PRV vaccine with three differential ELISAs |
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