Unique hexokinase messenger ribonucleic acids lacking the porin-binding domain are developmentally expressed in mouse spermatogenic cells
We have identified cDNAs representing three hexokinase mRNAs (Hk1-sa, Hk1-sb, Hk1-sc) by screening mouse spermatogenic cell cDNA libraries with a mouse hepatoma cell line hexokinase (Hk1) cDNA [Arora KK, Fanciulli M, Pederson PL. J Biol Chem 1990; 265:6481-6488]. Although all three cDNAs show 99% id...
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| Veröffentlicht in: | Biology of reproduction 1993-08, Vol.49 (2), p.191-203 |
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| container_title | Biology of reproduction |
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| creator | MORI, C WELCH, J. E FULCHER, K. D O'BRIEN, D. A EDDY, E. M |
| description | We have identified cDNAs representing three hexokinase mRNAs (Hk1-sa, Hk1-sb, Hk1-sc) by screening mouse spermatogenic cell
cDNA libraries with a mouse hepatoma cell line hexokinase (Hk1) cDNA [Arora KK, Fanciulli M, Pederson PL. J Biol Chem 1990;
265:6481-6488]. Although all three cDNAs show 99% identity to the somatic Hk1 cDNA sequence throughout most of their coding
region, they differ from this sequence at the 5' end. They contain a common spermatogenic cell-specific sequence and a sequence
unique to each cDNA immediately 5' to the common domain. However, they lack the porin-binding domain (PBD) present in this
region of Hk1, used for binding to a pore-forming protein in the outer mitochondrial membrane. These observations appear to
support a model proposed by others for hexokinase gene evolution in mammals. In addition, we found that Hk1-sb has an internal
sequence that is not present in Hk1, Hk1-sa, or Hk1-sc. Moreover, Hk1-sa and Hk1-sb transcripts are developmentally expressed
in mouse spermatogenic cells. Hk1-sa mRNA is first expressed during meiosis and continues to be present in postmeiotic germ
cells, while the more abundant Hk1-sb mRNA is detected only in postmeiotic germ cells. These and other findings suggest that
enzymes encoded by Hk1-sa, Hk1-sb, and Hk1-sc are present only in spermatogenic cells. |
| doi_str_mv | 10.1095/biolreprod49.2.191 |
| format | Article |
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cDNA libraries with a mouse hepatoma cell line hexokinase (Hk1) cDNA [Arora KK, Fanciulli M, Pederson PL. J Biol Chem 1990;
265:6481-6488]. Although all three cDNAs show 99% identity to the somatic Hk1 cDNA sequence throughout most of their coding
region, they differ from this sequence at the 5' end. They contain a common spermatogenic cell-specific sequence and a sequence
unique to each cDNA immediately 5' to the common domain. However, they lack the porin-binding domain (PBD) present in this
region of Hk1, used for binding to a pore-forming protein in the outer mitochondrial membrane. These observations appear to
support a model proposed by others for hexokinase gene evolution in mammals. In addition, we found that Hk1-sb has an internal
sequence that is not present in Hk1, Hk1-sa, or Hk1-sc. Moreover, Hk1-sa and Hk1-sb transcripts are developmentally expressed
in mouse spermatogenic cells. Hk1-sa mRNA is first expressed during meiosis and continues to be present in postmeiotic germ
cells, while the more abundant Hk1-sb mRNA is detected only in postmeiotic germ cells. These and other findings suggest that
enzymes encoded by Hk1-sa, Hk1-sb, and Hk1-sc are present only in spermatogenic cells.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod49.2.191</identifier><identifier>PMID: 8396993</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Aging ; Animals ; Bacterial Outer Membrane Proteins - metabolism ; Base Sequence ; Binding Sites ; Biological and medical sciences ; Blotting, Northern ; DNA - chemistry ; DNA - isolation & purification ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Hexokinase - chemistry ; Hexokinase - genetics ; Hexokinase - metabolism ; In Situ Hybridization ; Male ; Mammalian male genital system ; Mice ; Molecular Sequence Data ; Morphology. Physiology ; Porins ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; Spermatids - enzymology ; Spermatocytes - enzymology ; Spermatozoa - enzymology ; Testis - chemistry ; Testis - growth & development ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 1993-08, Vol.49 (2), p.191-203</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4856294$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8396993$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MORI, C</creatorcontrib><creatorcontrib>WELCH, J. E</creatorcontrib><creatorcontrib>FULCHER, K. D</creatorcontrib><creatorcontrib>O'BRIEN, D. A</creatorcontrib><creatorcontrib>EDDY, E. M</creatorcontrib><title>Unique hexokinase messenger ribonucleic acids lacking the porin-binding domain are developmentally expressed in mouse spermatogenic cells</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>We have identified cDNAs representing three hexokinase mRNAs (Hk1-sa, Hk1-sb, Hk1-sc) by screening mouse spermatogenic cell
cDNA libraries with a mouse hepatoma cell line hexokinase (Hk1) cDNA [Arora KK, Fanciulli M, Pederson PL. J Biol Chem 1990;
265:6481-6488]. Although all three cDNAs show 99% identity to the somatic Hk1 cDNA sequence throughout most of their coding
region, they differ from this sequence at the 5' end. They contain a common spermatogenic cell-specific sequence and a sequence
unique to each cDNA immediately 5' to the common domain. However, they lack the porin-binding domain (PBD) present in this
region of Hk1, used for binding to a pore-forming protein in the outer mitochondrial membrane. These observations appear to
support a model proposed by others for hexokinase gene evolution in mammals. In addition, we found that Hk1-sb has an internal
sequence that is not present in Hk1, Hk1-sa, or Hk1-sc. Moreover, Hk1-sa and Hk1-sb transcripts are developmentally expressed
in mouse spermatogenic cells. Hk1-sa mRNA is first expressed during meiosis and continues to be present in postmeiotic germ
cells, while the more abundant Hk1-sb mRNA is detected only in postmeiotic germ cells. These and other findings suggest that
enzymes encoded by Hk1-sa, Hk1-sb, and Hk1-sc are present only in spermatogenic cells.</description><subject>Aging</subject><subject>Animals</subject><subject>Bacterial Outer Membrane Proteins - metabolism</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>DNA - chemistry</subject><subject>DNA - isolation & purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Hexokinase - chemistry</subject><subject>Hexokinase - genetics</subject><subject>Hexokinase - metabolism</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Morphology. Physiology</subject><subject>Porins</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>Spermatids - enzymology</subject><subject>Spermatocytes - enzymology</subject><subject>Spermatozoa - enzymology</subject><subject>Testis - chemistry</subject><subject>Testis - growth & development</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kU1v1DAQhi0EKtuFP4CE5ANwy-LPbHxEFVCkSlzoOZo4k43BH8HOsu1P4F_jVVecRvI8embmNSFvONtxZvTHwSWfcclpVGYndtzwZ2TDtTDNXrTdc7JhjLWNlK18Sa5L-ckYV1LIK3LVSdMaIzfk7310v49IZ3xIv1yEgjRgKRgPmGl2Q4pH69FZCtaNhXqwlTrQdUa6pOxiM7g4nl_GFMBFChnpiH_QpyVgXMH7R4oPSz47R1qBkI51RlkwB1jTAWN1W_S-vCIvJvAFX1_qltx_-fzj5ra5-_71282nu2bmHVsbi1PLuB60nKCzRsoBJ24GZkc7GYFS8b2ZNBgQVu8HUKYVHdOd4EwJbSWTW_LhyVtzq5eXtQ-unDeAiHW3fq-NkqrlFXx7AY9DwLFfsguQH_tLdrX_7tKHYsFPGaJ15T-mOt2KqtqS90_Y7A7zyWXsS6ixVKnsT6eTMr3o68_Jf0OokJE</recordid><startdate>199308</startdate><enddate>199308</enddate><creator>MORI, C</creator><creator>WELCH, J. E</creator><creator>FULCHER, K. D</creator><creator>O'BRIEN, D. A</creator><creator>EDDY, E. M</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199308</creationdate><title>Unique hexokinase messenger ribonucleic acids lacking the porin-binding domain are developmentally expressed in mouse spermatogenic cells</title><author>MORI, C ; WELCH, J. E ; FULCHER, K. D ; O'BRIEN, D. A ; EDDY, E. M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h180t-cef6015b53fa8c933bef19b0cdcf92e34179f5a9a2c57ba49628058210425c303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Aging</topic><topic>Animals</topic><topic>Bacterial Outer Membrane Proteins - metabolism</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>DNA - chemistry</topic><topic>DNA - isolation & purification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Hexokinase - chemistry</topic><topic>Hexokinase - genetics</topic><topic>Hexokinase - metabolism</topic><topic>In Situ Hybridization</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Morphology. Physiology</topic><topic>Porins</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - genetics</topic><topic>Spermatids - enzymology</topic><topic>Spermatocytes - enzymology</topic><topic>Spermatozoa - enzymology</topic><topic>Testis - chemistry</topic><topic>Testis - growth & development</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MORI, C</creatorcontrib><creatorcontrib>WELCH, J. E</creatorcontrib><creatorcontrib>FULCHER, K. D</creatorcontrib><creatorcontrib>O'BRIEN, D. A</creatorcontrib><creatorcontrib>EDDY, E. M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MORI, C</au><au>WELCH, J. E</au><au>FULCHER, K. D</au><au>O'BRIEN, D. A</au><au>EDDY, E. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Unique hexokinase messenger ribonucleic acids lacking the porin-binding domain are developmentally expressed in mouse spermatogenic cells</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1993-08</date><risdate>1993</risdate><volume>49</volume><issue>2</issue><spage>191</spage><epage>203</epage><pages>191-203</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>We have identified cDNAs representing three hexokinase mRNAs (Hk1-sa, Hk1-sb, Hk1-sc) by screening mouse spermatogenic cell
cDNA libraries with a mouse hepatoma cell line hexokinase (Hk1) cDNA [Arora KK, Fanciulli M, Pederson PL. J Biol Chem 1990;
265:6481-6488]. Although all three cDNAs show 99% identity to the somatic Hk1 cDNA sequence throughout most of their coding
region, they differ from this sequence at the 5' end. They contain a common spermatogenic cell-specific sequence and a sequence
unique to each cDNA immediately 5' to the common domain. However, they lack the porin-binding domain (PBD) present in this
region of Hk1, used for binding to a pore-forming protein in the outer mitochondrial membrane. These observations appear to
support a model proposed by others for hexokinase gene evolution in mammals. In addition, we found that Hk1-sb has an internal
sequence that is not present in Hk1, Hk1-sa, or Hk1-sc. Moreover, Hk1-sa and Hk1-sb transcripts are developmentally expressed
in mouse spermatogenic cells. Hk1-sa mRNA is first expressed during meiosis and continues to be present in postmeiotic germ
cells, while the more abundant Hk1-sb mRNA is detected only in postmeiotic germ cells. These and other findings suggest that
enzymes encoded by Hk1-sa, Hk1-sb, and Hk1-sc are present only in spermatogenic cells.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>8396993</pmid><doi>10.1095/biolreprod49.2.191</doi><tpages>13</tpages></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Aging Animals Bacterial Outer Membrane Proteins - metabolism Base Sequence Binding Sites Biological and medical sciences Blotting, Northern DNA - chemistry DNA - isolation & purification Fundamental and applied biological sciences. Psychology Gene Expression Hexokinase - chemistry Hexokinase - genetics Hexokinase - metabolism In Situ Hybridization Male Mammalian male genital system Mice Molecular Sequence Data Morphology. Physiology Porins RNA, Messenger - analysis RNA, Messenger - genetics Spermatids - enzymology Spermatocytes - enzymology Spermatozoa - enzymology Testis - chemistry Testis - growth & development Vertebrates: reproduction |
| title | Unique hexokinase messenger ribonucleic acids lacking the porin-binding domain are developmentally expressed in mouse spermatogenic cells |
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