Detection of GTP-binding proteins in barley aleurone protoplasts

We report the existence of several families of GTP-binding proteins in barley aleurone protoplasts. Partial purified plasma membrane proteins were separated by SDS-PAGE, transferred to a nitrocellulose filter and incubated with either antisera raised against a highly conserved animal G protein α sub...

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Veröffentlicht in:	FEBS letters 1993-08, Vol.329 (3), p.245-248
Hauptverfasser:	Wang, Mei, Sedee, Norbert J.A., Heidekamp, Freek, Snaar-Jagalska, B.Ewa
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Barley aleurone Binding and carrier proteins Binding Sites Biological and medical sciences Blotting, Western Cell biochemistry Cell Membrane - metabolism Cell physiology EGTA, ethylene glycol-bis(β-aminoethyl ether) N, N, N', N'-tetraacetic acid Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology G protein GTP-binding GTP-Binding Proteins - analysis GTP-Binding Proteins - metabolism Hordeum - chemistry MES, 2-(N-morpholino)ethanesulfonic acid Phytohormone PIPES, 1,4-piperazinediethanesulfonic acid Plant Growth Regulators - pharmacology Plant physiology and development Proteins Protoplasts - chemistry Signal Transduction Space life sciences Temperature Tris, tris(hydroxymethyl)aminomethane
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