Chemiluminescent detection of potato and pome fruit viroids by digoxigenin-labeled dot blot and tissue blot hybridization

A chemiluminescent molecular hybridization protocol was compared to 32P autoradiography for detecting potato spindle tuber viroid (PSTVd) and apple scar skin group viroids (ASSVd). Labeled cRNA probes for PSTVd and ASSVd were synthesized by SP6 RNA polymerase transcription using digoxigenin-11-UTP o...

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Veröffentlicht in:	Journal of virological methods 1993-07, Vol.43 (2), p.147-158
Hauptverfasser:	Podleckis, Edward V., Hammond, Rosemarie W., Hurtt, Suzanne S., Hadidi, Ahmed
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Apple scar skin viroid Biological and medical sciences Chemiluminescent Digoxigenin Fruit - microbiology Fundamental and applied biological sciences. Psychology Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control Hybridization Luminescent Measurements Nucleic Acid Hybridization - methods Phytopathology. Animal pests. Plant and forest protection Plant Diseases - microbiology Plant viruses and viroids Potato spindle tuber viroid RNA Probes RNA, Viral - isolation & purification Solanum tuberosum - microbiology Viroids - genetics Viroids - isolation & purification
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