Two‐dimensional impedance studies of BSA buoyant density separated human erythrocytes
Combined DC (Coulter Volume) and radio frequency impedance studies were performed on human erythrocytes which had been separated by buoyant density in linear, neutral, isotonic bovine serum albumin gradients. The individual buoyant density fractions showed no reproducible shift in volume with buoyan...
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| Veröffentlicht in: | Cytometry (New York, N.Y.) N.Y.), 1985-01, Vol.6 (1), p.13-21 |
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| container_title | Cytometry (New York, N.Y.) |
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| creator | Leif, R. C. Schwartz, S. Rodriguez, C. M. Pell‐Fernandez, L. Groves, M. Leif, S. B. Cayer, M. Crews, H. |
| description | Combined DC (Coulter Volume) and radio frequency impedance studies were performed on human erythrocytes which had been separated by buoyant density in linear, neutral, isotonic bovine serum albumin gradients. The individual buoyant density fractions showed no reproducible shift in volume with buoyant density but did show a shift with opacity, radio frequency impedance divided by dc impedance. This new electronic parameter of opacity can be related to cell age, since both it and cell age are directly related to buoyant density. This increase in opacity with buoyant density is correlated with a change in shape. |
| doi_str_mv | 10.1002/cyto.990060104 |
| format | Article |
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This increase in opacity with buoyant density is correlated with a change in shape.</description><identifier>ISSN: 0196-4763</identifier><identifier>EISSN: 1097-0320</identifier><identifier>DOI: 10.1002/cyto.990060104</identifier><identifier>PMID: 3967550</identifier><identifier>CODEN: CYTODQ</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adult ; Biological and medical sciences ; bovine serum albumin (BSA) ; buoyant density ; Cell Separation ; Centrifugation, Density Gradient ; Coulter electronic cell volume ; density gradient centrifugation ; Diverse techniques ; Electric Conductivity ; Erythrocyte Aging ; erythrocytes ; Erythrocytes - cytology ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; impedance ; Male ; Molecular and cellular biology ; opacity ; radio frequency ; Radio Waves ; Serum Albumin, Bovine</subject><ispartof>Cytometry (New York, N.Y.), 1985-01, Vol.6 (1), p.13-21</ispartof><rights>Copyright © 1985 Wiley‐Liss, Inc.</rights><rights>1985 INIST-CNRS</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3244-e03726a0aa843dd9f0a0427256aceb6f7a3503db60e096e9fd0fcf064a8b1593</citedby><cites>FETCH-LOGICAL-c3244-e03726a0aa843dd9f0a0427256aceb6f7a3503db60e096e9fd0fcf064a8b1593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9142269$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3967550$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leif, R. C.</creatorcontrib><creatorcontrib>Schwartz, S.</creatorcontrib><creatorcontrib>Rodriguez, C. M.</creatorcontrib><creatorcontrib>Pell‐Fernandez, L.</creatorcontrib><creatorcontrib>Groves, M.</creatorcontrib><creatorcontrib>Leif, S. B.</creatorcontrib><creatorcontrib>Cayer, M.</creatorcontrib><creatorcontrib>Crews, H.</creatorcontrib><title>Two‐dimensional impedance studies of BSA buoyant density separated human erythrocytes</title><title>Cytometry (New York, N.Y.)</title><addtitle>Cytometry</addtitle><description>Combined DC (Coulter Volume) and radio frequency impedance studies were performed on human erythrocytes which had been separated by buoyant density in linear, neutral, isotonic bovine serum albumin gradients. The individual buoyant density fractions showed no reproducible shift in volume with buoyant density but did show a shift with opacity, radio frequency impedance divided by dc impedance. This new electronic parameter of opacity can be related to cell age, since both it and cell age are directly related to buoyant density. This increase in opacity with buoyant density is correlated with a change in shape.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>bovine serum albumin (BSA)</subject><subject>buoyant density</subject><subject>Cell Separation</subject><subject>Centrifugation, Density Gradient</subject><subject>Coulter electronic cell volume</subject><subject>density gradient centrifugation</subject><subject>Diverse techniques</subject><subject>Electric Conductivity</subject><subject>Erythrocyte Aging</subject><subject>erythrocytes</subject><subject>Erythrocytes - cytology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>impedance</subject><subject>Male</subject><subject>Molecular and cellular biology</subject><subject>opacity</subject><subject>radio frequency</subject><subject>Radio Waves</subject><subject>Serum Albumin, Bovine</subject><issn>0196-4763</issn><issn>1097-0320</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMoun5cvQk5iLeu0yZNN0dd_ALBgwviqUybCVbaZk1apDd_gr_RX2KXXdajpzm8z8y8PIydxjCNAZLLcujcVGsABTHIHTaJQWcRiAR22QRirSKZKXHADkN4BwCtpNhn-0KrLE1hwl4Wn-7n69tUDbWhci3WvGqWZLAtiYeuNxUF7iy_fr7iRe8GbDtuVmg38EBL9NiR4W99gy0nP3Rv3o2NKByzPYt1oJPNPGKL25vF_D56fLp7mF89RqVIpIwIRJYoBMSZFMZoCwgyyZJUYUmFshmKFIQpFNBYnbQ1YEsLSuKsiFMtjtjF-uzSu4-eQpc3VSiprrEl14c8G5nZiI_gdA2W3oXgyeZLXzXohzyGfCUyX4nMtyLHhbPN5b5oyGzxjbkxP9_kGEqsrR-NVWGL6VgmiVoV1Gvss6pp-OdpPn9dPP1V-AVXDY7n</recordid><startdate>198501</startdate><enddate>198501</enddate><creator>Leif, R. 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Psychology</topic><topic>Humans</topic><topic>impedance</topic><topic>Male</topic><topic>Molecular and cellular biology</topic><topic>opacity</topic><topic>radio frequency</topic><topic>Radio Waves</topic><topic>Serum Albumin, Bovine</topic><toplevel>online_resources</toplevel><creatorcontrib>Leif, R. C.</creatorcontrib><creatorcontrib>Schwartz, S.</creatorcontrib><creatorcontrib>Rodriguez, C. M.</creatorcontrib><creatorcontrib>Pell‐Fernandez, L.</creatorcontrib><creatorcontrib>Groves, M.</creatorcontrib><creatorcontrib>Leif, S. 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B.</au><au>Cayer, M.</au><au>Crews, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two‐dimensional impedance studies of BSA buoyant density separated human erythrocytes</atitle><jtitle>Cytometry (New York, N.Y.)</jtitle><addtitle>Cytometry</addtitle><date>1985-01</date><risdate>1985</risdate><volume>6</volume><issue>1</issue><spage>13</spage><epage>21</epage><pages>13-21</pages><issn>0196-4763</issn><eissn>1097-0320</eissn><coden>CYTODQ</coden><abstract>Combined DC (Coulter Volume) and radio frequency impedance studies were performed on human erythrocytes which had been separated by buoyant density in linear, neutral, isotonic bovine serum albumin gradients. The individual buoyant density fractions showed no reproducible shift in volume with buoyant density but did show a shift with opacity, radio frequency impedance divided by dc impedance. 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| identifier | ISSN: 0196-4763 |
| ispartof | Cytometry (New York, N.Y.), 1985-01, Vol.6 (1), p.13-21 |
| issn | 0196-4763 1097-0320 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75938064 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adult Biological and medical sciences bovine serum albumin (BSA) buoyant density Cell Separation Centrifugation, Density Gradient Coulter electronic cell volume density gradient centrifugation Diverse techniques Electric Conductivity Erythrocyte Aging erythrocytes Erythrocytes - cytology Female Fundamental and applied biological sciences. Psychology Humans impedance Male Molecular and cellular biology opacity radio frequency Radio Waves Serum Albumin, Bovine |
| title | Two‐dimensional impedance studies of BSA buoyant density separated human erythrocytes |
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