Simultaneous multi-site EPR spectroscopy in vivo
A gradient technique to measure electron paramagnetic resonance spectra simultaneously at several different locations is described. The technique is based on the use of point probes containing paramagnetic centers. The value of the magnetic field gradient is chosen to be sufficient to separate the E...
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| Veröffentlicht in: | Magnetic resonance in medicine 1993-08, Vol.30 (2), p.213-220 |
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| container_title | Magnetic resonance in medicine |
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| creator | Smirnov, Alex I. Norby, Shong-Wan Clarkson, R. B. Walczak, Ted Swartz, Harold M. |
| description | A gradient technique to measure electron paramagnetic resonance spectra simultaneously at several different locations is described. The technique is based on the use of point probes containing paramagnetic centers. The value of the magnetic field gradient is chosen to be sufficient to separate the EPR signals from the different paramagnetic probes yet at the same time small enough to change only minimally the shape of individual signals. The conditions to apply this technique are considered in detail. When experimental data have a high signal‐to‐noise ratio, the lineshape distortion induced by the gradient can be corrected with the aid of a known distribution function of paramagnetic centers within the probe. The maximum entropy deconvolution algorithm is successfully applied for the correction of significantly distorted lines. The technique is experimentally tested and applied to measure the concentration of oxygen in hypertrophied rat myocardium and normal rat kidney in vivo by low frequency EPR (L‐band, 1.2 GHz). No types of EPR oxygen‐sensitive probes‐lithium phthalocyanine crystals and synthetic carbohydrate chars‐were used. |
| doi_str_mv | 10.1002/mrm.1910300210 |
| format | Article |
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B. ; Walczak, Ted ; Swartz, Harold M.</creator><creatorcontrib>Smirnov, Alex I. ; Norby, Shong-Wan ; Clarkson, R. B. ; Walczak, Ted ; Swartz, Harold M.</creatorcontrib><description>A gradient technique to measure electron paramagnetic resonance spectra simultaneously at several different locations is described. The technique is based on the use of point probes containing paramagnetic centers. The value of the magnetic field gradient is chosen to be sufficient to separate the EPR signals from the different paramagnetic probes yet at the same time small enough to change only minimally the shape of individual signals. The conditions to apply this technique are considered in detail. When experimental data have a high signal‐to‐noise ratio, the lineshape distortion induced by the gradient can be corrected with the aid of a known distribution function of paramagnetic centers within the probe. The maximum entropy deconvolution algorithm is successfully applied for the correction of significantly distorted lines. The technique is experimentally tested and applied to measure the concentration of oxygen in hypertrophied rat myocardium and normal rat kidney in vivo by low frequency EPR (L‐band, 1.2 GHz). No types of EPR oxygen‐sensitive probes‐lithium phthalocyanine crystals and synthetic carbohydrate chars‐were used.</description><identifier>ISSN: 0740-3194</identifier><identifier>EISSN: 1522-2594</identifier><identifier>DOI: 10.1002/mrm.1910300210</identifier><identifier>PMID: 8396190</identifier><identifier>CODEN: MRMEEN</identifier><language>eng</language><publisher>Baltimore: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological and medical sciences ; carbonaceous materials ; Cardiomegaly - metabolism ; electron paramagnetic resonance ; Electron Spin Resonance Spectroscopy - methods ; Female ; Investigative techniques, diagnostic techniques (general aspects) ; Kidney - metabolism ; Medical sciences ; Miscellaneous. Technology ; myocardium ; oximetry ; Oxygen - analysis ; Pathology. Cytology. Biochemistry. Spectrometry. 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B.</creatorcontrib><creatorcontrib>Walczak, Ted</creatorcontrib><creatorcontrib>Swartz, Harold M.</creatorcontrib><title>Simultaneous multi-site EPR spectroscopy in vivo</title><title>Magnetic resonance in medicine</title><addtitle>Magn. Reson. Med</addtitle><description>A gradient technique to measure electron paramagnetic resonance spectra simultaneously at several different locations is described. The technique is based on the use of point probes containing paramagnetic centers. The value of the magnetic field gradient is chosen to be sufficient to separate the EPR signals from the different paramagnetic probes yet at the same time small enough to change only minimally the shape of individual signals. The conditions to apply this technique are considered in detail. When experimental data have a high signal‐to‐noise ratio, the lineshape distortion induced by the gradient can be corrected with the aid of a known distribution function of paramagnetic centers within the probe. The maximum entropy deconvolution algorithm is successfully applied for the correction of significantly distorted lines. The technique is experimentally tested and applied to measure the concentration of oxygen in hypertrophied rat myocardium and normal rat kidney in vivo by low frequency EPR (L‐band, 1.2 GHz). No types of EPR oxygen‐sensitive probes‐lithium phthalocyanine crystals and synthetic carbohydrate chars‐were used.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>carbonaceous materials</subject><subject>Cardiomegaly - metabolism</subject><subject>electron paramagnetic resonance</subject><subject>Electron Spin Resonance Spectroscopy - methods</subject><subject>Female</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Kidney - metabolism</subject><subject>Medical sciences</subject><subject>Miscellaneous. Technology</subject><subject>myocardium</subject><subject>oximetry</subject><subject>Oxygen - analysis</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>0740-3194</issn><issn>1522-2594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL1PwzAQxS0EKqWwsiFlQGwp_nY8IlQKUstHC2K0bONIhqQJcVLof0-qREVMTHen-727pwfAKYJjBCG-zKt8jCSCpB0Q3ANDxDCOMZN0HwyhoDAmSNJDcBTCO4RQSkEHYJAQyZGEQwCXPm-yWq9c0YRo2_o4-NpFk8dFFEpn66oItig3kV9Fa78ujsFBqrPgTvo6Ai83k-fr23j2ML27vprFliQJjKnT0gpJDBfYMGFSSpFLuZPaGuOIgTRJCRWOEU44NkRSoxG1LNGcc2Y0GYGL7m5ZFZ-NC7XKfbAuyzqrSjBJBCW4BccdaFunoXKpKiuf62qjEFTbiFQbkfqNqBWc9Zcbk7u3Hd5n0u7P-70OVmdppVfWhx1GE8wlYS0mO-zLZ27zz1M1X8z_WIg7rQ-1-95pdfWhuCCCqdf7qRJ4idgTpgqTH8_rjN0</recordid><startdate>199308</startdate><enddate>199308</enddate><creator>Smirnov, Alex I.</creator><creator>Norby, Shong-Wan</creator><creator>Clarkson, R. 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B. ; Walczak, Ted ; Swartz, Harold M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3880-4ea9c793b672b57bf441ef6e9acbbe3b048f347e536362b394ba14c58a6665ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>carbonaceous materials</topic><topic>Cardiomegaly - metabolism</topic><topic>electron paramagnetic resonance</topic><topic>Electron Spin Resonance Spectroscopy - methods</topic><topic>Female</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Kidney - metabolism</topic><topic>Medical sciences</topic><topic>Miscellaneous. Technology</topic><topic>myocardium</topic><topic>oximetry</topic><topic>Oxygen - analysis</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smirnov, Alex I.</creatorcontrib><creatorcontrib>Norby, Shong-Wan</creatorcontrib><creatorcontrib>Clarkson, R. B.</creatorcontrib><creatorcontrib>Walczak, Ted</creatorcontrib><creatorcontrib>Swartz, Harold M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Magnetic resonance in medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smirnov, Alex I.</au><au>Norby, Shong-Wan</au><au>Clarkson, R. B.</au><au>Walczak, Ted</au><au>Swartz, Harold M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous multi-site EPR spectroscopy in vivo</atitle><jtitle>Magnetic resonance in medicine</jtitle><addtitle>Magn. Reson. Med</addtitle><date>1993-08</date><risdate>1993</risdate><volume>30</volume><issue>2</issue><spage>213</spage><epage>220</epage><pages>213-220</pages><issn>0740-3194</issn><eissn>1522-2594</eissn><coden>MRMEEN</coden><abstract>A gradient technique to measure electron paramagnetic resonance spectra simultaneously at several different locations is described. The technique is based on the use of point probes containing paramagnetic centers. The value of the magnetic field gradient is chosen to be sufficient to separate the EPR signals from the different paramagnetic probes yet at the same time small enough to change only minimally the shape of individual signals. The conditions to apply this technique are considered in detail. When experimental data have a high signal‐to‐noise ratio, the lineshape distortion induced by the gradient can be corrected with the aid of a known distribution function of paramagnetic centers within the probe. The maximum entropy deconvolution algorithm is successfully applied for the correction of significantly distorted lines. The technique is experimentally tested and applied to measure the concentration of oxygen in hypertrophied rat myocardium and normal rat kidney in vivo by low frequency EPR (L‐band, 1.2 GHz). No types of EPR oxygen‐sensitive probes‐lithium phthalocyanine crystals and synthetic carbohydrate chars‐were used.</abstract><cop>Baltimore</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>8396190</pmid><doi>10.1002/mrm.1910300210</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Wiley Journals |
| subjects | Animals Biological and medical sciences carbonaceous materials Cardiomegaly - metabolism electron paramagnetic resonance Electron Spin Resonance Spectroscopy - methods Female Investigative techniques, diagnostic techniques (general aspects) Kidney - metabolism Medical sciences Miscellaneous. Technology myocardium oximetry Oxygen - analysis Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Rats Rats, Sprague-Dawley |
| title | Simultaneous multi-site EPR spectroscopy in vivo |
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