In vitro phosphorylation of proteins tightly bound to DNA by protein kinase NII

1. 1. Highly purified DNA from calf thymus was phosphorylated with protein kinase NII. 2. 2. Digestion with proteinase K of this DNA demonstrates proteins as phosphorylated component. 3. 3. Gel filtration chromatography on Bio-Gel A-0.5m gel column shows a major protein peak between 50 and 70 kDa. 4...

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Veröffentlicht in:	International journal of biochemistry 1993-07, Vol.25 (7), p.1035-1039
Hauptverfasser:	Piccinini, Gina, Bramucci, Massimo, Maccari, Ennio, Miano, Antonino, Amici, Domenico, Gianfranceschi, Gian Luigi, Cardellini, Elena
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Binding and carrier proteins Biological and medical sciences Caseins - metabolism Cattle Chromatography, Gel Chromatography, Thin Layer DNA - metabolism DNA-Binding Proteins - metabolism Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Phosphorylation Protein Kinases - metabolism Proteins Substrate Specificity
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