Diacylglycerol lipase activity in human platelet intracellular and surface membranes: Some kinetic properties and fatty acid specificity

Diacyl glycerol lipase activity has been examined of intracellular and surface membranes isolated from human blood platelets by free flow electrophoresis. Enzyme activity is present on both membranes but is activated at different substrate concentrations ( K m 14 μM and 140 μM for intracellular and...

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Veröffentlicht in:	FEBS letters 1985-01, Vol.180 (1), p.95-101
Hauptverfasser:	Authi, Kalwant S., Lagarde, Michel, Crawford, Neville
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Blood Platelets - enzymology Blood Platelets - ultrastructure Cell Membrane - enzymology Centrifugation, Density Gradient Diacylglycerol lipase Egtazic Acid - pharmacology Enzymes and enzyme inhibitors Fatty acid specificity fatty acids Fatty Acids - metabolism Fundamental and applied biological sciences. Psychology Glutathione - pharmacology Human platelet membrane Humans Hydrolases Intracellular Membranes - enzymology Kinetics Leupeptins - pharmacology lipoprotein lipase Lipoprotein Lipase - blood man platelets Substrate Specificity Trifluoperazine - pharmacology
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