Comparative analysis of the germ cell markers c-KIT, SSEA-1 and VASA in testicular biopsies from secretory and obstructive azoospermias
Testicular biopsy is needed to confirm diagnosis in azoospermic patients and to recover spermatozoa, if possible. This report aims to quantitatively analyse the germline markers stage-specific embryonic antigen (SSEA-1), c-KIT and VASA in testicular biopsies with distinct azoospermic aetiologies. Tw...
Gespeichert in:
| Veröffentlicht in: | Molecular human reproduction 2010-11, Vol.16 (11), p.811-817 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 817 |
|---|---|
| container_issue | 11 |
| container_start_page | 811 |
| container_title | Molecular human reproduction |
| container_volume | 16 |
| creator | Medrano, J.V. Marqués-Marí, A.I. Aguilar, C.E. Riboldi, M. Garrido, N. Martínez-Romero, A. O'Connor, E. Gil-Salom, M. Simón, C. |
| description | Testicular biopsy is needed to confirm diagnosis in azoospermic patients and to recover spermatozoa, if possible. This report aims to quantitatively analyse the germline markers stage-specific embryonic antigen (SSEA-1), c-KIT and VASA in testicular biopsies with distinct azoospermic aetiologies. Twenty-three testicular biopsies were analysed by flow cytometry and RT-qPCR for c-KIT, SSEA-1 and VASA. In all the Sertoli cell-only (SCO) samples, significantly lower VASA mRNA expression and fewer VASA+ cells were found compared with obstructive controls. Maturation arrest (MA) cases showed significant differences only with the non-mosaic SCO samples when compared for VASA mRNA expression and percentage of VASA+ cells, but not with the mosaics. However, the normalized VASA–KIT parameter obtained by subtracting the percentage of c-KIT+ cells from the percentage of VASA+ cells showed significant differences between the MA and all the SCO samples. RT-qPCR consistently found differences for the VASA expression between SCO mosaic and non-mosaic samples. However, by flow cytometry, only VASA–KIT showed significant differences between them. Conversely, the percentage of SSEA-1+ cells revealed no inter-group differences. In conclusion, testicular biopsies display different expression profiles for c-KIT and VASA depending on the azoospermic aetiology. These results can be used as a complementary tool to create new molecular categories for diagnoses in azoospermic patients, particularly useful to discriminate between mosaic and non-mosaic SCO patients. |
| doi_str_mv | 10.1093/molehr/gaq044 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_759325862</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>759325862</sourcerecordid><originalsourceid>FETCH-LOGICAL-c435t-5addc84e91290b0c0af43341a7e4489853650b5b03edfda8cb98f0af09ff2b1d3</originalsourceid><addsrcrecordid>eNpFkMFu1DAQhi0EoqVw5Ip840LacWwnznG1Km3VCg7bIsTFcpxxa0jWqZ0glhfoa-M2pT3NSPPpn5mPkPcMDhk0_GgIPd7Eo2tzC0K8IPtMVFCUAuqXuee5bxpR75E3Kf0EYHUp1WuyV4IEBZXcJ3frMIwmmsn_Rmq2pt8ln2hwdLpBeo1xoBb7ng4m_sKYqC3Ozy4_0c3meFWwzHf022qzon5LJ0yTt3NvIm19GJPHRF0MA01oI04h7h7w0KYpznZZ9zeENOYd3qS35JUzfcJ3j_WAXH0-vlyfFhdfT87Wq4vCCi6nQpqus0pgw8oGWrBgnOBcMFOjEKpRklcSWtkCx851Rtm2US5D0DhXtqzjB-TjkjvGcDvnm_Xg0_2LZothTrqWDc-OqjKTxULaGFKK6PQYffaw0wz0vXq9qNeL-sx_eEye2wG7J_q_6-dAnyb88zTPZnVV81rq0-8_tILyi1IntS75P2BSkgk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>759325862</pqid></control><display><type>article</type><title>Comparative analysis of the germ cell markers c-KIT, SSEA-1 and VASA in testicular biopsies from secretory and obstructive azoospermias</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Medrano, J.V. ; Marqués-Marí, A.I. ; Aguilar, C.E. ; Riboldi, M. ; Garrido, N. ; Martínez-Romero, A. ; O'Connor, E. ; Gil-Salom, M. ; Simón, C.</creator><creatorcontrib>Medrano, J.V. ; Marqués-Marí, A.I. ; Aguilar, C.E. ; Riboldi, M. ; Garrido, N. ; Martínez-Romero, A. ; O'Connor, E. ; Gil-Salom, M. ; Simón, C.</creatorcontrib><description>Testicular biopsy is needed to confirm diagnosis in azoospermic patients and to recover spermatozoa, if possible. This report aims to quantitatively analyse the germline markers stage-specific embryonic antigen (SSEA-1), c-KIT and VASA in testicular biopsies with distinct azoospermic aetiologies. Twenty-three testicular biopsies were analysed by flow cytometry and RT-qPCR for c-KIT, SSEA-1 and VASA. In all the Sertoli cell-only (SCO) samples, significantly lower VASA mRNA expression and fewer VASA+ cells were found compared with obstructive controls. Maturation arrest (MA) cases showed significant differences only with the non-mosaic SCO samples when compared for VASA mRNA expression and percentage of VASA+ cells, but not with the mosaics. However, the normalized VASA–KIT parameter obtained by subtracting the percentage of c-KIT+ cells from the percentage of VASA+ cells showed significant differences between the MA and all the SCO samples. RT-qPCR consistently found differences for the VASA expression between SCO mosaic and non-mosaic samples. However, by flow cytometry, only VASA–KIT showed significant differences between them. Conversely, the percentage of SSEA-1+ cells revealed no inter-group differences. In conclusion, testicular biopsies display different expression profiles for c-KIT and VASA depending on the azoospermic aetiology. These results can be used as a complementary tool to create new molecular categories for diagnoses in azoospermic patients, particularly useful to discriminate between mosaic and non-mosaic SCO patients.</description><identifier>ISSN: 1360-9947</identifier><identifier>EISSN: 1460-2407</identifier><identifier>DOI: 10.1093/molehr/gaq044</identifier><identifier>PMID: 20508065</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adult ; Azoospermia - diagnosis ; Azoospermia - pathology ; Biomarkers - metabolism ; c-KIT ; DEAD-box RNA Helicases - metabolism ; Gene Expression Profiling ; Humans ; Lewis X Antigen - metabolism ; Male ; male infertility ; Sertoli Cells - pathology ; SSEA-1 ; testicular biopsy ; VASA</subject><ispartof>Molecular human reproduction, 2010-11, Vol.16 (11), p.811-817</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-5addc84e91290b0c0af43341a7e4489853650b5b03edfda8cb98f0af09ff2b1d3</citedby><cites>FETCH-LOGICAL-c435t-5addc84e91290b0c0af43341a7e4489853650b5b03edfda8cb98f0af09ff2b1d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20508065$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Medrano, J.V.</creatorcontrib><creatorcontrib>Marqués-Marí, A.I.</creatorcontrib><creatorcontrib>Aguilar, C.E.</creatorcontrib><creatorcontrib>Riboldi, M.</creatorcontrib><creatorcontrib>Garrido, N.</creatorcontrib><creatorcontrib>Martínez-Romero, A.</creatorcontrib><creatorcontrib>O'Connor, E.</creatorcontrib><creatorcontrib>Gil-Salom, M.</creatorcontrib><creatorcontrib>Simón, C.</creatorcontrib><title>Comparative analysis of the germ cell markers c-KIT, SSEA-1 and VASA in testicular biopsies from secretory and obstructive azoospermias</title><title>Molecular human reproduction</title><addtitle>Mol Hum Reprod</addtitle><description>Testicular biopsy is needed to confirm diagnosis in azoospermic patients and to recover spermatozoa, if possible. This report aims to quantitatively analyse the germline markers stage-specific embryonic antigen (SSEA-1), c-KIT and VASA in testicular biopsies with distinct azoospermic aetiologies. Twenty-three testicular biopsies were analysed by flow cytometry and RT-qPCR for c-KIT, SSEA-1 and VASA. In all the Sertoli cell-only (SCO) samples, significantly lower VASA mRNA expression and fewer VASA+ cells were found compared with obstructive controls. Maturation arrest (MA) cases showed significant differences only with the non-mosaic SCO samples when compared for VASA mRNA expression and percentage of VASA+ cells, but not with the mosaics. However, the normalized VASA–KIT parameter obtained by subtracting the percentage of c-KIT+ cells from the percentage of VASA+ cells showed significant differences between the MA and all the SCO samples. RT-qPCR consistently found differences for the VASA expression between SCO mosaic and non-mosaic samples. However, by flow cytometry, only VASA–KIT showed significant differences between them. Conversely, the percentage of SSEA-1+ cells revealed no inter-group differences. In conclusion, testicular biopsies display different expression profiles for c-KIT and VASA depending on the azoospermic aetiology. These results can be used as a complementary tool to create new molecular categories for diagnoses in azoospermic patients, particularly useful to discriminate between mosaic and non-mosaic SCO patients.</description><subject>Adult</subject><subject>Azoospermia - diagnosis</subject><subject>Azoospermia - pathology</subject><subject>Biomarkers - metabolism</subject><subject>c-KIT</subject><subject>DEAD-box RNA Helicases - metabolism</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Lewis X Antigen - metabolism</subject><subject>Male</subject><subject>male infertility</subject><subject>Sertoli Cells - pathology</subject><subject>SSEA-1</subject><subject>testicular biopsy</subject><subject>VASA</subject><issn>1360-9947</issn><issn>1460-2407</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMFu1DAQhi0EoqVw5Ip840LacWwnznG1Km3VCg7bIsTFcpxxa0jWqZ0glhfoa-M2pT3NSPPpn5mPkPcMDhk0_GgIPd7Eo2tzC0K8IPtMVFCUAuqXuee5bxpR75E3Kf0EYHUp1WuyV4IEBZXcJ3frMIwmmsn_Rmq2pt8ln2hwdLpBeo1xoBb7ng4m_sKYqC3Ozy4_0c3meFWwzHf022qzon5LJ0yTt3NvIm19GJPHRF0MA01oI04h7h7w0KYpznZZ9zeENOYd3qS35JUzfcJ3j_WAXH0-vlyfFhdfT87Wq4vCCi6nQpqus0pgw8oGWrBgnOBcMFOjEKpRklcSWtkCx851Rtm2US5D0DhXtqzjB-TjkjvGcDvnm_Xg0_2LZothTrqWDc-OqjKTxULaGFKK6PQYffaw0wz0vXq9qNeL-sx_eEye2wG7J_q_6-dAnyb88zTPZnVV81rq0-8_tILyi1IntS75P2BSkgk</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Medrano, J.V.</creator><creator>Marqués-Marí, A.I.</creator><creator>Aguilar, C.E.</creator><creator>Riboldi, M.</creator><creator>Garrido, N.</creator><creator>Martínez-Romero, A.</creator><creator>O'Connor, E.</creator><creator>Gil-Salom, M.</creator><creator>Simón, C.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20101101</creationdate><title>Comparative analysis of the germ cell markers c-KIT, SSEA-1 and VASA in testicular biopsies from secretory and obstructive azoospermias</title><author>Medrano, J.V. ; Marqués-Marí, A.I. ; Aguilar, C.E. ; Riboldi, M. ; Garrido, N. ; Martínez-Romero, A. ; O'Connor, E. ; Gil-Salom, M. ; Simón, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-5addc84e91290b0c0af43341a7e4489853650b5b03edfda8cb98f0af09ff2b1d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Azoospermia - diagnosis</topic><topic>Azoospermia - pathology</topic><topic>Biomarkers - metabolism</topic><topic>c-KIT</topic><topic>DEAD-box RNA Helicases - metabolism</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Lewis X Antigen - metabolism</topic><topic>Male</topic><topic>male infertility</topic><topic>Sertoli Cells - pathology</topic><topic>SSEA-1</topic><topic>testicular biopsy</topic><topic>VASA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Medrano, J.V.</creatorcontrib><creatorcontrib>Marqués-Marí, A.I.</creatorcontrib><creatorcontrib>Aguilar, C.E.</creatorcontrib><creatorcontrib>Riboldi, M.</creatorcontrib><creatorcontrib>Garrido, N.</creatorcontrib><creatorcontrib>Martínez-Romero, A.</creatorcontrib><creatorcontrib>O'Connor, E.</creatorcontrib><creatorcontrib>Gil-Salom, M.</creatorcontrib><creatorcontrib>Simón, C.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular human reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Medrano, J.V.</au><au>Marqués-Marí, A.I.</au><au>Aguilar, C.E.</au><au>Riboldi, M.</au><au>Garrido, N.</au><au>Martínez-Romero, A.</au><au>O'Connor, E.</au><au>Gil-Salom, M.</au><au>Simón, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative analysis of the germ cell markers c-KIT, SSEA-1 and VASA in testicular biopsies from secretory and obstructive azoospermias</atitle><jtitle>Molecular human reproduction</jtitle><addtitle>Mol Hum Reprod</addtitle><date>2010-11-01</date><risdate>2010</risdate><volume>16</volume><issue>11</issue><spage>811</spage><epage>817</epage><pages>811-817</pages><issn>1360-9947</issn><eissn>1460-2407</eissn><abstract>Testicular biopsy is needed to confirm diagnosis in azoospermic patients and to recover spermatozoa, if possible. This report aims to quantitatively analyse the germline markers stage-specific embryonic antigen (SSEA-1), c-KIT and VASA in testicular biopsies with distinct azoospermic aetiologies. Twenty-three testicular biopsies were analysed by flow cytometry and RT-qPCR for c-KIT, SSEA-1 and VASA. In all the Sertoli cell-only (SCO) samples, significantly lower VASA mRNA expression and fewer VASA+ cells were found compared with obstructive controls. Maturation arrest (MA) cases showed significant differences only with the non-mosaic SCO samples when compared for VASA mRNA expression and percentage of VASA+ cells, but not with the mosaics. However, the normalized VASA–KIT parameter obtained by subtracting the percentage of c-KIT+ cells from the percentage of VASA+ cells showed significant differences between the MA and all the SCO samples. RT-qPCR consistently found differences for the VASA expression between SCO mosaic and non-mosaic samples. However, by flow cytometry, only VASA–KIT showed significant differences between them. Conversely, the percentage of SSEA-1+ cells revealed no inter-group differences. In conclusion, testicular biopsies display different expression profiles for c-KIT and VASA depending on the azoospermic aetiology. These results can be used as a complementary tool to create new molecular categories for diagnoses in azoospermic patients, particularly useful to discriminate between mosaic and non-mosaic SCO patients.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>20508065</pmid><doi>10.1093/molehr/gaq044</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1360-9947 |
| ispartof | Molecular human reproduction, 2010-11, Vol.16 (11), p.811-817 |
| issn | 1360-9947 1460-2407 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_759325862 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adult Azoospermia - diagnosis Azoospermia - pathology Biomarkers - metabolism c-KIT DEAD-box RNA Helicases - metabolism Gene Expression Profiling Humans Lewis X Antigen - metabolism Male male infertility Sertoli Cells - pathology SSEA-1 testicular biopsy VASA |
| title | Comparative analysis of the germ cell markers c-KIT, SSEA-1 and VASA in testicular biopsies from secretory and obstructive azoospermias |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T07%3A54%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20analysis%20of%20the%20germ%20cell%20markers%20c-KIT,%20SSEA-1%20and%20VASA%20in%20testicular%20biopsies%20from%20secretory%20and%20obstructive%20azoospermias&rft.jtitle=Molecular%20human%20reproduction&rft.au=Medrano,%20J.V.&rft.date=2010-11-01&rft.volume=16&rft.issue=11&rft.spage=811&rft.epage=817&rft.pages=811-817&rft.issn=1360-9947&rft.eissn=1460-2407&rft_id=info:doi/10.1093/molehr/gaq044&rft_dat=%3Cproquest_cross%3E759325862%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=759325862&rft_id=info:pmid/20508065&rfr_iscdi=true |