Enhanced Expression of the Sweet Taste Receptors and Alpha-gustducin in Reactive Astrocytes of the Rat Hippocampus Following Ischemic Injury
The heterodimeric sweet taste receptors, T1R2 and T1R3, have recently been proposed to be associated with the brain glucose sensor. To identify whether sweet taste signaling is regulated in response to an ischemic injury inducing acute impairment of glucose metabolism, we investigated the spatiotemp...
Gespeichert in:
Veröffentlicht in: | Neurochemical research 2010-10, Vol.35 (10), p.1628-1634 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 1634 |
---|---|
container_issue | 10 |
container_start_page | 1628 |
container_title | Neurochemical research |
container_volume | 35 |
creator | Shin, Yoo-Jin Park, Joo-Hee Choi, Jeong-Sun Chun, Myung-Hoon Moon, Young Wha Lee, Mun-Yong |
description | The heterodimeric sweet taste receptors, T1R2 and T1R3, have recently been proposed to be associated with the brain glucose sensor. To identify whether sweet taste signaling is regulated in response to an ischemic injury inducing acute impairment of glucose metabolism, we investigated the spatiotemporal expression of the sweet taste receptors and their associated taste-specific G-protein α-gustducin in the rat hippocampus after ischemia. The expression profiles of both receptor subunits and α-gustducin shared overlapping expression patterns in sham-operated and ischemic hippocampi. Constitutive expression of both receptors and α-gustducin was localized in neurons of the pyramidal cell and granule cell layers, but their upregulation was detected in reactive astrocytes in ischemic hippocampi. Immunoblot analysis confirmed the immmunohistochemically determined temporal patterns of sweet-taste signaling proteins. These results suggest that the expression of sweet taste signaling proteins in astrocytes might be regulated in response to altered extracellular levels of glucose following an ischemic insult. |
doi_str_mv | 10.1007/s11064-010-0223-2 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_759320863</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2146419981</sourcerecordid><originalsourceid>FETCH-LOGICAL-c468t-a57ac5f873cab7ba540b8427581be4f76c45f91b235606d64839fcd7b35a77803</originalsourceid><addsrcrecordid>eNqNkc-KFDEQh4Mo7rj6AF4kePHUmj-dpPs4LLPuwIIwrucmna6e6aEnaVPpXecdfGgzzK6CIAgFdaivfkXxEfKWs4-cMfMJOWe6LBhnBRNCFuIZWXBlZKFrJp-TBZN5KnnNLsgrxD3LIBP8JbkQTNXa6HpBfq78znoHHV39mCIgDsHT0NO0A_r1ASDRO4sJ6AYcTClEpNZ3dDlOO1tsZ0zd7AZPc23AujTcA11iisEdE-BTzsYmejNMU3D2MM1Ir8M4hofBb-ka3Q4Og6Nrv5_j8TV50dsR4c1jvyTfrld3VzfF7ZfP66vlbeFKXaXCKmOd6isjnW1Na1XJ2qoURlW8hbI32pWqr3krpNJMd7qsZN27zrRSWWMqJi_Jh3PuFMP3GTA1hwEdjKP1EGZsjKqlYJWW_0EqbqSoVSbf_0Xuwxx9fiNDJVeM1yJD_Ay5GBAj9M0Uh4ONx4az5qS0OSttsqnmpLQ57bx7DJ7bA3S_N54cZkCcAcwjv4X45_K_U38Bg06sPw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>754150192</pqid></control><display><type>article</type><title>Enhanced Expression of the Sweet Taste Receptors and Alpha-gustducin in Reactive Astrocytes of the Rat Hippocampus Following Ischemic Injury</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Shin, Yoo-Jin ; Park, Joo-Hee ; Choi, Jeong-Sun ; Chun, Myung-Hoon ; Moon, Young Wha ; Lee, Mun-Yong</creator><creatorcontrib>Shin, Yoo-Jin ; Park, Joo-Hee ; Choi, Jeong-Sun ; Chun, Myung-Hoon ; Moon, Young Wha ; Lee, Mun-Yong</creatorcontrib><description>The heterodimeric sweet taste receptors, T1R2 and T1R3, have recently been proposed to be associated with the brain glucose sensor. To identify whether sweet taste signaling is regulated in response to an ischemic injury inducing acute impairment of glucose metabolism, we investigated the spatiotemporal expression of the sweet taste receptors and their associated taste-specific G-protein α-gustducin in the rat hippocampus after ischemia. The expression profiles of both receptor subunits and α-gustducin shared overlapping expression patterns in sham-operated and ischemic hippocampi. Constitutive expression of both receptors and α-gustducin was localized in neurons of the pyramidal cell and granule cell layers, but their upregulation was detected in reactive astrocytes in ischemic hippocampi. Immunoblot analysis confirmed the immmunohistochemically determined temporal patterns of sweet-taste signaling proteins. These results suggest that the expression of sweet taste signaling proteins in astrocytes might be regulated in response to altered extracellular levels of glucose following an ischemic insult.</description><identifier>ISSN: 0364-3190</identifier><identifier>EISSN: 1573-6903</identifier><identifier>DOI: 10.1007/s11064-010-0223-2</identifier><identifier>PMID: 20596769</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Animals ; Astrocytes - metabolism ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Hippocampus - metabolism ; Immunoblotting ; Immunohistochemistry ; Ischemic Attack, Transient - metabolism ; Male ; Neurochemistry ; Neurology ; Neurons - metabolism ; Neurosciences ; Original Paper ; Protein Subunits - biosynthesis ; Rats ; Rats, Sprague-Dawley ; Receptors, G-Protein-Coupled - biosynthesis ; Taste ; Time Factors ; Transducin - biosynthesis</subject><ispartof>Neurochemical research, 2010-10, Vol.35 (10), p.1628-1634</ispartof><rights>Springer Science+Business Media, LLC 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-a57ac5f873cab7ba540b8427581be4f76c45f91b235606d64839fcd7b35a77803</citedby><cites>FETCH-LOGICAL-c468t-a57ac5f873cab7ba540b8427581be4f76c45f91b235606d64839fcd7b35a77803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11064-010-0223-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11064-010-0223-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20596769$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shin, Yoo-Jin</creatorcontrib><creatorcontrib>Park, Joo-Hee</creatorcontrib><creatorcontrib>Choi, Jeong-Sun</creatorcontrib><creatorcontrib>Chun, Myung-Hoon</creatorcontrib><creatorcontrib>Moon, Young Wha</creatorcontrib><creatorcontrib>Lee, Mun-Yong</creatorcontrib><title>Enhanced Expression of the Sweet Taste Receptors and Alpha-gustducin in Reactive Astrocytes of the Rat Hippocampus Following Ischemic Injury</title><title>Neurochemical research</title><addtitle>Neurochem Res</addtitle><addtitle>Neurochem Res</addtitle><description>The heterodimeric sweet taste receptors, T1R2 and T1R3, have recently been proposed to be associated with the brain glucose sensor. To identify whether sweet taste signaling is regulated in response to an ischemic injury inducing acute impairment of glucose metabolism, we investigated the spatiotemporal expression of the sweet taste receptors and their associated taste-specific G-protein α-gustducin in the rat hippocampus after ischemia. The expression profiles of both receptor subunits and α-gustducin shared overlapping expression patterns in sham-operated and ischemic hippocampi. Constitutive expression of both receptors and α-gustducin was localized in neurons of the pyramidal cell and granule cell layers, but their upregulation was detected in reactive astrocytes in ischemic hippocampi. Immunoblot analysis confirmed the immmunohistochemically determined temporal patterns of sweet-taste signaling proteins. These results suggest that the expression of sweet taste signaling proteins in astrocytes might be regulated in response to altered extracellular levels of glucose following an ischemic insult.</description><subject>Animals</subject><subject>Astrocytes - metabolism</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Hippocampus - metabolism</subject><subject>Immunoblotting</subject><subject>Immunohistochemistry</subject><subject>Ischemic Attack, Transient - metabolism</subject><subject>Male</subject><subject>Neurochemistry</subject><subject>Neurology</subject><subject>Neurons - metabolism</subject><subject>Neurosciences</subject><subject>Original Paper</subject><subject>Protein Subunits - biosynthesis</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, G-Protein-Coupled - biosynthesis</subject><subject>Taste</subject><subject>Time Factors</subject><subject>Transducin - biosynthesis</subject><issn>0364-3190</issn><issn>1573-6903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkc-KFDEQh4Mo7rj6AF4kePHUmj-dpPs4LLPuwIIwrucmna6e6aEnaVPpXecdfGgzzK6CIAgFdaivfkXxEfKWs4-cMfMJOWe6LBhnBRNCFuIZWXBlZKFrJp-TBZN5KnnNLsgrxD3LIBP8JbkQTNXa6HpBfq78znoHHV39mCIgDsHT0NO0A_r1ASDRO4sJ6AYcTClEpNZ3dDlOO1tsZ0zd7AZPc23AujTcA11iisEdE-BTzsYmejNMU3D2MM1Ir8M4hofBb-ka3Q4Og6Nrv5_j8TV50dsR4c1jvyTfrld3VzfF7ZfP66vlbeFKXaXCKmOd6isjnW1Na1XJ2qoURlW8hbI32pWqr3krpNJMd7qsZN27zrRSWWMqJi_Jh3PuFMP3GTA1hwEdjKP1EGZsjKqlYJWW_0EqbqSoVSbf_0Xuwxx9fiNDJVeM1yJD_Ay5GBAj9M0Uh4ONx4az5qS0OSttsqnmpLQ57bx7DJ7bA3S_N54cZkCcAcwjv4X45_K_U38Bg06sPw</recordid><startdate>20101001</startdate><enddate>20101001</enddate><creator>Shin, Yoo-Jin</creator><creator>Park, Joo-Hee</creator><creator>Choi, Jeong-Sun</creator><creator>Chun, Myung-Hoon</creator><creator>Moon, Young Wha</creator><creator>Lee, Mun-Yong</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20101001</creationdate><title>Enhanced Expression of the Sweet Taste Receptors and Alpha-gustducin in Reactive Astrocytes of the Rat Hippocampus Following Ischemic Injury</title><author>Shin, Yoo-Jin ; Park, Joo-Hee ; Choi, Jeong-Sun ; Chun, Myung-Hoon ; Moon, Young Wha ; Lee, Mun-Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-a57ac5f873cab7ba540b8427581be4f76c45f91b235606d64839fcd7b35a77803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Astrocytes - metabolism</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Biology</topic><topic>Hippocampus - metabolism</topic><topic>Immunoblotting</topic><topic>Immunohistochemistry</topic><topic>Ischemic Attack, Transient - metabolism</topic><topic>Male</topic><topic>Neurochemistry</topic><topic>Neurology</topic><topic>Neurons - metabolism</topic><topic>Neurosciences</topic><topic>Original Paper</topic><topic>Protein Subunits - biosynthesis</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, G-Protein-Coupled - biosynthesis</topic><topic>Taste</topic><topic>Time Factors</topic><topic>Transducin - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shin, Yoo-Jin</creatorcontrib><creatorcontrib>Park, Joo-Hee</creatorcontrib><creatorcontrib>Choi, Jeong-Sun</creatorcontrib><creatorcontrib>Chun, Myung-Hoon</creatorcontrib><creatorcontrib>Moon, Young Wha</creatorcontrib><creatorcontrib>Lee, Mun-Yong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>Proquest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Neurochemical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shin, Yoo-Jin</au><au>Park, Joo-Hee</au><au>Choi, Jeong-Sun</au><au>Chun, Myung-Hoon</au><au>Moon, Young Wha</au><au>Lee, Mun-Yong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced Expression of the Sweet Taste Receptors and Alpha-gustducin in Reactive Astrocytes of the Rat Hippocampus Following Ischemic Injury</atitle><jtitle>Neurochemical research</jtitle><stitle>Neurochem Res</stitle><addtitle>Neurochem Res</addtitle><date>2010-10-01</date><risdate>2010</risdate><volume>35</volume><issue>10</issue><spage>1628</spage><epage>1634</epage><pages>1628-1634</pages><issn>0364-3190</issn><eissn>1573-6903</eissn><abstract>The heterodimeric sweet taste receptors, T1R2 and T1R3, have recently been proposed to be associated with the brain glucose sensor. To identify whether sweet taste signaling is regulated in response to an ischemic injury inducing acute impairment of glucose metabolism, we investigated the spatiotemporal expression of the sweet taste receptors and their associated taste-specific G-protein α-gustducin in the rat hippocampus after ischemia. The expression profiles of both receptor subunits and α-gustducin shared overlapping expression patterns in sham-operated and ischemic hippocampi. Constitutive expression of both receptors and α-gustducin was localized in neurons of the pyramidal cell and granule cell layers, but their upregulation was detected in reactive astrocytes in ischemic hippocampi. Immunoblot analysis confirmed the immmunohistochemically determined temporal patterns of sweet-taste signaling proteins. These results suggest that the expression of sweet taste signaling proteins in astrocytes might be regulated in response to altered extracellular levels of glucose following an ischemic insult.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>20596769</pmid><doi>10.1007/s11064-010-0223-2</doi><tpages>7</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0364-3190 |
ispartof | Neurochemical research, 2010-10, Vol.35 (10), p.1628-1634 |
issn | 0364-3190 1573-6903 |
language | eng |
recordid | cdi_proquest_miscellaneous_759320863 |
source | MEDLINE; SpringerNature Journals |
subjects | Animals Astrocytes - metabolism Biochemistry Biomedical and Life Sciences Biomedicine Cell Biology Hippocampus - metabolism Immunoblotting Immunohistochemistry Ischemic Attack, Transient - metabolism Male Neurochemistry Neurology Neurons - metabolism Neurosciences Original Paper Protein Subunits - biosynthesis Rats Rats, Sprague-Dawley Receptors, G-Protein-Coupled - biosynthesis Taste Time Factors Transducin - biosynthesis |
title | Enhanced Expression of the Sweet Taste Receptors and Alpha-gustducin in Reactive Astrocytes of the Rat Hippocampus Following Ischemic Injury |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-14T20%3A05%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enhanced%20Expression%20of%20the%20Sweet%20Taste%20Receptors%20and%20Alpha-gustducin%20in%20Reactive%20Astrocytes%20of%20the%20Rat%20Hippocampus%20Following%20Ischemic%20Injury&rft.jtitle=Neurochemical%20research&rft.au=Shin,%20Yoo-Jin&rft.date=2010-10-01&rft.volume=35&rft.issue=10&rft.spage=1628&rft.epage=1634&rft.pages=1628-1634&rft.issn=0364-3190&rft.eissn=1573-6903&rft_id=info:doi/10.1007/s11064-010-0223-2&rft_dat=%3Cproquest_cross%3E2146419981%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=754150192&rft_id=info:pmid/20596769&rfr_iscdi=true |