Enhanced Expression of the Sweet Taste Receptors and Alpha-gustducin in Reactive Astrocytes of the Rat Hippocampus Following Ischemic Injury

The heterodimeric sweet taste receptors, T1R2 and T1R3, have recently been proposed to be associated with the brain glucose sensor. To identify whether sweet taste signaling is regulated in response to an ischemic injury inducing acute impairment of glucose metabolism, we investigated the spatiotemp...

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Veröffentlicht in:Neurochemical research 2010-10, Vol.35 (10), p.1628-1634
Hauptverfasser: Shin, Yoo-Jin, Park, Joo-Hee, Choi, Jeong-Sun, Chun, Myung-Hoon, Moon, Young Wha, Lee, Mun-Yong
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container_end_page 1634
container_issue 10
container_start_page 1628
container_title Neurochemical research
container_volume 35
creator Shin, Yoo-Jin
Park, Joo-Hee
Choi, Jeong-Sun
Chun, Myung-Hoon
Moon, Young Wha
Lee, Mun-Yong
description The heterodimeric sweet taste receptors, T1R2 and T1R3, have recently been proposed to be associated with the brain glucose sensor. To identify whether sweet taste signaling is regulated in response to an ischemic injury inducing acute impairment of glucose metabolism, we investigated the spatiotemporal expression of the sweet taste receptors and their associated taste-specific G-protein α-gustducin in the rat hippocampus after ischemia. The expression profiles of both receptor subunits and α-gustducin shared overlapping expression patterns in sham-operated and ischemic hippocampi. Constitutive expression of both receptors and α-gustducin was localized in neurons of the pyramidal cell and granule cell layers, but their upregulation was detected in reactive astrocytes in ischemic hippocampi. Immunoblot analysis confirmed the immmunohistochemically determined temporal patterns of sweet-taste signaling proteins. These results suggest that the expression of sweet taste signaling proteins in astrocytes might be regulated in response to altered extracellular levels of glucose following an ischemic insult.
doi_str_mv 10.1007/s11064-010-0223-2
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subjects Animals
Astrocytes - metabolism
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cell Biology
Hippocampus - metabolism
Immunoblotting
Immunohistochemistry
Ischemic Attack, Transient - metabolism
Male
Neurochemistry
Neurology
Neurons - metabolism
Neurosciences
Original Paper
Protein Subunits - biosynthesis
Rats
Rats, Sprague-Dawley
Receptors, G-Protein-Coupled - biosynthesis
Taste
Time Factors
Transducin - biosynthesis
title Enhanced Expression of the Sweet Taste Receptors and Alpha-gustducin in Reactive Astrocytes of the Rat Hippocampus Following Ischemic Injury
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