The rne gene and ribonuclease E

The cloned rne + gene complements temperature sensitive RNase E mutations and directs the synthesis of a polypeptide. In vitro the RNA transcribed from the rne gene directs the synthesis of a number of polypeptides, one of which is identical in size to the in vivo product of the rne gene. A rabbit r...

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Veröffentlicht in:	Biochimie 1993, Vol.75 (6), p.473-479
Hauptverfasser:	Miczak, A., Apirion, D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences DNA, Bacterial - genetics Endoribonucleases - genetics Enzyme Stability Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Expression Genes. Genome Hot Temperature Molecular and cellular biology Molecular genetics Plasmids post-translational changes Rabbits Reticulocytes - metabolism RNase E temperature sensitivity Transcription, Genetic
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container_title	Biochimie
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creator	Miczak, A. Apirion, D.
description	The cloned rne + gene complements temperature sensitive RNase E mutations and directs the synthesis of a polypeptide. In vitro the RNA transcribed from the rne gene directs the synthesis of a number of polypeptides, one of which is identical in size to the in vivo product of the rne gene. A rabbit reticulocyte cell free extract programmed with this RNA produced RNase E activity. Thus, it is evident that the rne gene is the structural gene for RNase E. However, the in vivo product of the cloned RNase E gene is more thermolabile than the chromosomal gene product. When cells containing the rne plasmid were treated with chloramphenicol, the pre-existing RNase E became less heat labile with time. This leads to the suggestion that in the cell RNase E undergoes post-translational modification(s).
doi_str_mv	10.1016/0300-9084(93)90113-7
format	Article
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Psychology</topic><topic>Gene Expression</topic><topic>Genes. Genome</topic><topic>Hot Temperature</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Plasmids</topic><topic>post-translational changes</topic><topic>Rabbits</topic><topic>Reticulocytes - metabolism</topic><topic>RNase E</topic><topic>temperature sensitivity</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miczak, A.</creatorcontrib><creatorcontrib>Apirion, D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochimie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miczak, A.</au><au>Apirion, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The rne gene and ribonuclease E</atitle><jtitle>Biochimie</jtitle><addtitle>Biochimie</addtitle><date>1993</date><risdate>1993</risdate><volume>75</volume><issue>6</issue><spage>473</spage><epage>479</epage><pages>473-479</pages><issn>0300-9084</issn><eissn>1638-6183</eissn><coden>BICMBE</coden><abstract>The cloned rne + gene complements temperature sensitive RNase E mutations and directs the synthesis of a polypeptide. 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subjects	Animals Biological and medical sciences DNA, Bacterial - genetics Endoribonucleases - genetics Enzyme Stability Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Expression Genes. Genome Hot Temperature Molecular and cellular biology Molecular genetics Plasmids post-translational changes Rabbits Reticulocytes - metabolism RNase E temperature sensitivity Transcription, Genetic
title	The rne gene and ribonuclease E
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