Use of magnetically oriented orthogonal collagen scaffolds for hemi-corneal reconstruction and regeneration
Abstract We recently showed that the highly organized architecture of the corneal stroma could be reproduced using scaffolds consisting of orthogonally aligned multilayers of collagen fibrils prepared using a high magnetic field. Here we show that such scaffolds permit the reconstruction in vitro of...
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| Veröffentlicht in: | Biomaterials 2010-11, Vol.31 (32), p.8313-8322 |
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| creator | Builles, Nicolas Janin-Manificat, Hélène Malbouyres, Marilyne Justin, Virginie Rovère, Marie-Rose Pellegrini, Graziella Torbet, Jim Hulmes, David J.S Burillon, Carole Damour, Odile Ruggiero, Florence |
| description | Abstract We recently showed that the highly organized architecture of the corneal stroma could be reproduced using scaffolds consisting of orthogonally aligned multilayers of collagen fibrils prepared using a high magnetic field. Here we show that such scaffolds permit the reconstruction in vitro of human hemi-corneas (stroma + epithelium), using primary human keratocytes and limbal stem cell derived human keratinocytes. On the surface of these hemi-corneas, a well-differentiated epithelium was formed, as determined both histologically and ultrastructurally and by the expression of characteristic markers. Within the stroma, the keratocytes aligned with the directions of the fibrils in the scaffold and synthesized a new extracellular matrix with typical collagen markers and small, uniform diameter fibrils. Finally, in vivo experiments using a rabbit model showed that these orthogonally oriented multi-layer scaffolds could be used to repair the anterior region of the stroma, leading to re-epithelialization and recovery of both transparency and ultrastructural organization. |
| doi_str_mv | 10.1016/j.biomaterials.2010.07.066 |
| format | Article |
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Here we show that such scaffolds permit the reconstruction in vitro of human hemi-corneas (stroma + epithelium), using primary human keratocytes and limbal stem cell derived human keratinocytes. On the surface of these hemi-corneas, a well-differentiated epithelium was formed, as determined both histologically and ultrastructurally and by the expression of characteristic markers. Within the stroma, the keratocytes aligned with the directions of the fibrils in the scaffold and synthesized a new extracellular matrix with typical collagen markers and small, uniform diameter fibrils. Finally, in vivo experiments using a rabbit model showed that these orthogonally oriented multi-layer scaffolds could be used to repair the anterior region of the stroma, leading to re-epithelialization and recovery of both transparency and ultrastructural organization.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2010.07.066</identifier><identifier>PMID: 20708260</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Alignment ; Animals ; Cells, Cultured ; Collagen ; Collagen - chemistry ; Collagen - metabolism ; Cornea ; Cornea - cytology ; Cornea - physiology ; Cornea - ultrastructure ; Dentistry ; Humans ; Implants, Experimental ; Keratinocytes - cytology ; Keratinocytes - metabolism ; Magnetics ; Male ; Rabbits ; Regeneration ; Scaffold ; Stem Cells - cytology ; Tissue Scaffolds - chemistry</subject><ispartof>Biomaterials, 2010-11, Vol.31 (32), p.8313-8322</ispartof><rights>Elsevier Ltd</rights><rights>2010 Elsevier Ltd</rights><rights>Copyright (c) 2010 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-47c3db7e325690a2e244a98c4e356f11e70eb76a4ab9d7802034905f44a287a3</citedby><cites>FETCH-LOGICAL-c532t-47c3db7e325690a2e244a98c4e356f11e70eb76a4ab9d7802034905f44a287a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biomaterials.2010.07.066$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20708260$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Builles, Nicolas</creatorcontrib><creatorcontrib>Janin-Manificat, Hélène</creatorcontrib><creatorcontrib>Malbouyres, Marilyne</creatorcontrib><creatorcontrib>Justin, Virginie</creatorcontrib><creatorcontrib>Rovère, Marie-Rose</creatorcontrib><creatorcontrib>Pellegrini, Graziella</creatorcontrib><creatorcontrib>Torbet, Jim</creatorcontrib><creatorcontrib>Hulmes, David J.S</creatorcontrib><creatorcontrib>Burillon, Carole</creatorcontrib><creatorcontrib>Damour, Odile</creatorcontrib><creatorcontrib>Ruggiero, Florence</creatorcontrib><title>Use of magnetically oriented orthogonal collagen scaffolds for hemi-corneal reconstruction and regeneration</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract We recently showed that the highly organized architecture of the corneal stroma could be reproduced using scaffolds consisting of orthogonally aligned multilayers of collagen fibrils prepared using a high magnetic field. Here we show that such scaffolds permit the reconstruction in vitro of human hemi-corneas (stroma + epithelium), using primary human keratocytes and limbal stem cell derived human keratinocytes. On the surface of these hemi-corneas, a well-differentiated epithelium was formed, as determined both histologically and ultrastructurally and by the expression of characteristic markers. Within the stroma, the keratocytes aligned with the directions of the fibrils in the scaffold and synthesized a new extracellular matrix with typical collagen markers and small, uniform diameter fibrils. Finally, in vivo experiments using a rabbit model showed that these orthogonally oriented multi-layer scaffolds could be used to repair the anterior region of the stroma, leading to re-epithelialization and recovery of both transparency and ultrastructural organization.</description><subject>Advanced Basic Science</subject><subject>Alignment</subject><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Collagen</subject><subject>Collagen - chemistry</subject><subject>Collagen - metabolism</subject><subject>Cornea</subject><subject>Cornea - cytology</subject><subject>Cornea - physiology</subject><subject>Cornea - ultrastructure</subject><subject>Dentistry</subject><subject>Humans</subject><subject>Implants, Experimental</subject><subject>Keratinocytes - cytology</subject><subject>Keratinocytes - metabolism</subject><subject>Magnetics</subject><subject>Male</subject><subject>Rabbits</subject><subject>Regeneration</subject><subject>Scaffold</subject><subject>Stem Cells - cytology</subject><subject>Tissue Scaffolds - chemistry</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksFu1DAQhi0EotvCK6CIC6csY8exYw5IqECpVKmHlrPlOJOtt4ldbAdp3x5HWxDqqSfb4_-fGc9nQt5T2FKg4uN-27swm4zRmSltGZQLkFsQ4gXZ0E52daugfUk2QDmrlaDshJymtIdyBs5ekxMGEjomYEPufyaswljNZucxO2um6VCF6NBnHMom34Vd8GaqbJgms0NfJWvGMUxDqsYQqzucXW1D9Fg0EW3wKcfFZhd8ZfxQQsWD0ayBN-TVWBrGt4_rGbn9_u32_Ed9dX1xef7lqrZtw3LNpW2GXmLDWqHAMGScG9VZjk0rRkpRAvZSGG56NcgOGDS8vHcsKtZJ05yRD8e0DzH8WjBlPbtksbTvMSxJy1Y1lHeie4ayUUpwpYry01FpY0gp4qgfoptNPGgKeoWi9_p_KHqFokHqAqWY3z2WWfoZh3_WvxSK4OtRgGUqvx1GnWwhYHFwZaRZD8E9r87nJ2ns5PzK9B4PmPZhiX71UJ2YBn2zfo_1d1AAUIy3zR_FNbsN</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Builles, Nicolas</creator><creator>Janin-Manificat, Hélène</creator><creator>Malbouyres, Marilyne</creator><creator>Justin, Virginie</creator><creator>Rovère, Marie-Rose</creator><creator>Pellegrini, Graziella</creator><creator>Torbet, Jim</creator><creator>Hulmes, David J.S</creator><creator>Burillon, Carole</creator><creator>Damour, Odile</creator><creator>Ruggiero, Florence</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20101101</creationdate><title>Use of magnetically oriented orthogonal collagen scaffolds for hemi-corneal reconstruction and regeneration</title><author>Builles, Nicolas ; Janin-Manificat, Hélène ; Malbouyres, Marilyne ; Justin, Virginie ; Rovère, Marie-Rose ; Pellegrini, Graziella ; Torbet, Jim ; Hulmes, David J.S ; Burillon, Carole ; Damour, Odile ; Ruggiero, Florence</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-47c3db7e325690a2e244a98c4e356f11e70eb76a4ab9d7802034905f44a287a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Advanced Basic Science</topic><topic>Alignment</topic><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Collagen</topic><topic>Collagen - chemistry</topic><topic>Collagen - metabolism</topic><topic>Cornea</topic><topic>Cornea - cytology</topic><topic>Cornea - physiology</topic><topic>Cornea - ultrastructure</topic><topic>Dentistry</topic><topic>Humans</topic><topic>Implants, Experimental</topic><topic>Keratinocytes - cytology</topic><topic>Keratinocytes - metabolism</topic><topic>Magnetics</topic><topic>Male</topic><topic>Rabbits</topic><topic>Regeneration</topic><topic>Scaffold</topic><topic>Stem Cells - cytology</topic><topic>Tissue Scaffolds - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Builles, Nicolas</creatorcontrib><creatorcontrib>Janin-Manificat, Hélène</creatorcontrib><creatorcontrib>Malbouyres, Marilyne</creatorcontrib><creatorcontrib>Justin, Virginie</creatorcontrib><creatorcontrib>Rovère, Marie-Rose</creatorcontrib><creatorcontrib>Pellegrini, Graziella</creatorcontrib><creatorcontrib>Torbet, Jim</creatorcontrib><creatorcontrib>Hulmes, David J.S</creatorcontrib><creatorcontrib>Burillon, Carole</creatorcontrib><creatorcontrib>Damour, Odile</creatorcontrib><creatorcontrib>Ruggiero, Florence</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Builles, Nicolas</au><au>Janin-Manificat, Hélène</au><au>Malbouyres, Marilyne</au><au>Justin, Virginie</au><au>Rovère, Marie-Rose</au><au>Pellegrini, Graziella</au><au>Torbet, Jim</au><au>Hulmes, David J.S</au><au>Burillon, Carole</au><au>Damour, Odile</au><au>Ruggiero, Florence</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of magnetically oriented orthogonal collagen scaffolds for hemi-corneal reconstruction and regeneration</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2010-11-01</date><risdate>2010</risdate><volume>31</volume><issue>32</issue><spage>8313</spage><epage>8322</epage><pages>8313-8322</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract We recently showed that the highly organized architecture of the corneal stroma could be reproduced using scaffolds consisting of orthogonally aligned multilayers of collagen fibrils prepared using a high magnetic field. Here we show that such scaffolds permit the reconstruction in vitro of human hemi-corneas (stroma + epithelium), using primary human keratocytes and limbal stem cell derived human keratinocytes. On the surface of these hemi-corneas, a well-differentiated epithelium was formed, as determined both histologically and ultrastructurally and by the expression of characteristic markers. Within the stroma, the keratocytes aligned with the directions of the fibrils in the scaffold and synthesized a new extracellular matrix with typical collagen markers and small, uniform diameter fibrils. 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| subjects | Advanced Basic Science Alignment Animals Cells, Cultured Collagen Collagen - chemistry Collagen - metabolism Cornea Cornea - cytology Cornea - physiology Cornea - ultrastructure Dentistry Humans Implants, Experimental Keratinocytes - cytology Keratinocytes - metabolism Magnetics Male Rabbits Regeneration Scaffold Stem Cells - cytology Tissue Scaffolds - chemistry |
| title | Use of magnetically oriented orthogonal collagen scaffolds for hemi-corneal reconstruction and regeneration |
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