Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey
Propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties attributed to the presence of polyphenols. In this study, we determined the antioxidant properties of lyophilized aqueous extract of propolis (LAEP) from Erzurum province of Turkey and corre...
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| Veröffentlicht in: | Food and chemical toxicology 2010-08, Vol.48 (8), p.2227-2238 |
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| creator | Gülçin, İlhami Bursal, Ercan Şehitoğlu, M. Hilal Bilsel, Mine Gören, Ahmet C. |
| description | Propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties attributed to the presence of polyphenols. In this study, we determined the antioxidant properties of lyophilized aqueous extract of propolis (LAEP) from Erzurum province of Turkey and correlated the values with total levels of polyphenolic compounds. In order to estimate the capacity of LAEP to act as antioxidants, we studied its 1,1-diphenyl-2-picryl-hydrazyl radicals (DPPH
), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals (ABTS
+), N,N-dimethyl-
p-phenylenediamine radicals (DMPD
+), superoxide anion radicals (
O
2
-
) and hydrogen peroxide (H
2O
2) scavenging activity, total antioxidant activity, ferric ions (Fe
3+) and cupric ions (Cu
2+) reducing ability, ferrous ions (Fe
2+) chelating activity. LAEP inhibited 93.2% lipid peroxidation of a linoleic acid emulsion at 30
μg/mL concentration. On the other hand, BHA, BHT, α-tocopherol and trolox displayed 83.3%, 82.1%, 68.1% and 81.3% inhibition of peroxidation at the same concentration, respectively. Quantitative amounts of caffeic acid, ferulic acid, syringic acid, ellagic acid, quercetin, α-tocopherol, pyrogallol,
p-hydroxybenzoic acid, vanillin,
p-coumaric acid, gallic acid and ascorbic acid were detected by high performance liquid chromatography and tandem mass spectrometry (LC–MS/MS). This study will bring an innovation for further studies with regard to the antioxidant properties of LAEP. |
| doi_str_mv | 10.1016/j.fct.2010.05.053 |
| format | Article |
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), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals (ABTS
+), N,N-dimethyl-
p-phenylenediamine radicals (DMPD
+), superoxide anion radicals (
O
2
-
) and hydrogen peroxide (H
2O
2) scavenging activity, total antioxidant activity, ferric ions (Fe
3+) and cupric ions (Cu
2+) reducing ability, ferrous ions (Fe
2+) chelating activity. LAEP inhibited 93.2% lipid peroxidation of a linoleic acid emulsion at 30
μg/mL concentration. On the other hand, BHA, BHT, α-tocopherol and trolox displayed 83.3%, 82.1%, 68.1% and 81.3% inhibition of peroxidation at the same concentration, respectively. Quantitative amounts of caffeic acid, ferulic acid, syringic acid, ellagic acid, quercetin, α-tocopherol, pyrogallol,
p-hydroxybenzoic acid, vanillin,
p-coumaric acid, gallic acid and ascorbic acid were detected by high performance liquid chromatography and tandem mass spectrometry (LC–MS/MS). This study will bring an innovation for further studies with regard to the antioxidant properties of LAEP.</description><identifier>ISSN: 0278-6915</identifier><identifier>EISSN: 1873-6351</identifier><identifier>DOI: 10.1016/j.fct.2010.05.053</identifier><identifier>PMID: 20685228</identifier><identifier>CODEN: FCTOD7</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Anti-Infective Agents - analysis ; Antioxidant activity ; Antioxidants - analysis ; Biological and medical sciences ; Chromatography, High Pressure Liquid ; Flavonoids - analysis ; Free Radical Scavengers - analysis ; Free Radicals - metabolism ; General pharmacology ; LC–MS/MS ; Limit of Detection ; Lipid Peroxidation ; Medical sciences ; Metal chelating ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Phenols - analysis ; Polyphenol content ; Polyphenols ; Propolis ; Propolis - chemistry ; Radical scavenging ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry ; Turkey</subject><ispartof>Food and chemical toxicology, 2010-08, Vol.48 (8), p.2227-2238</ispartof><rights>2010 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright (c) 2010 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c480t-68f57634d58368a4a7b9b51fff50afc357e48530617841a68f59f74ae761b1783</citedby><cites>FETCH-LOGICAL-c480t-68f57634d58368a4a7b9b51fff50afc357e48530617841a68f59f74ae761b1783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0278691510003315$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23117237$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20685228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gülçin, İlhami</creatorcontrib><creatorcontrib>Bursal, Ercan</creatorcontrib><creatorcontrib>Şehitoğlu, M. Hilal</creatorcontrib><creatorcontrib>Bilsel, Mine</creatorcontrib><creatorcontrib>Gören, Ahmet C.</creatorcontrib><title>Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey</title><title>Food and chemical toxicology</title><addtitle>Food Chem Toxicol</addtitle><description>Propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties attributed to the presence of polyphenols. In this study, we determined the antioxidant properties of lyophilized aqueous extract of propolis (LAEP) from Erzurum province of Turkey and correlated the values with total levels of polyphenolic compounds. In order to estimate the capacity of LAEP to act as antioxidants, we studied its 1,1-diphenyl-2-picryl-hydrazyl radicals (DPPH
), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals (ABTS
+), N,N-dimethyl-
p-phenylenediamine radicals (DMPD
+), superoxide anion radicals (
O
2
-
) and hydrogen peroxide (H
2O
2) scavenging activity, total antioxidant activity, ferric ions (Fe
3+) and cupric ions (Cu
2+) reducing ability, ferrous ions (Fe
2+) chelating activity. LAEP inhibited 93.2% lipid peroxidation of a linoleic acid emulsion at 30
μg/mL concentration. On the other hand, BHA, BHT, α-tocopherol and trolox displayed 83.3%, 82.1%, 68.1% and 81.3% inhibition of peroxidation at the same concentration, respectively. Quantitative amounts of caffeic acid, ferulic acid, syringic acid, ellagic acid, quercetin, α-tocopherol, pyrogallol,
p-hydroxybenzoic acid, vanillin,
p-coumaric acid, gallic acid and ascorbic acid were detected by high performance liquid chromatography and tandem mass spectrometry (LC–MS/MS). This study will bring an innovation for further studies with regard to the antioxidant properties of LAEP.</description><subject>Anti-Infective Agents - analysis</subject><subject>Antioxidant activity</subject><subject>Antioxidants - analysis</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Flavonoids - analysis</subject><subject>Free Radical Scavengers - analysis</subject><subject>Free Radicals - metabolism</subject><subject>General pharmacology</subject><subject>LC–MS/MS</subject><subject>Limit of Detection</subject><subject>Lipid Peroxidation</subject><subject>Medical sciences</subject><subject>Metal chelating</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Phenols - analysis</subject><subject>Polyphenol content</subject><subject>Polyphenols</subject><subject>Propolis</subject><subject>Propolis - chemistry</subject><subject>Radical scavenging</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Tandem Mass Spectrometry</subject><subject>Turkey</subject><issn>0278-6915</issn><issn>1873-6351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EotvCD-CCfEFcmsWO448VJ1SVD6kSHMrZ8jq26sWJg-1UTX89E-0CN4Q01mjsZ8av5kXoFSVbSqh4d9h6W7ctgZpwCPYEbaiSrBGM06doQ1qpGrGj_Aydl3IghEgqxXN01hKheNuqDYrfUlymOzemiG0aqxtrwWbs4dSQHkIPGRtbw32oC04exyVNdyGGRwfMz9mluWD3UDMw6_OU05RiKNjnNODr_DjnebjEt3P-4ZYX6Jk3sbiXp3yBvn-8vr363Nx8_fTl6sNNYztFaiOU51KwrueKCWU6I_e7Pafee06Mt4xL1ynOiKBSddSs-M7Lzjgp6B7u2AV6e5wLakBiqXoIxboYzbjq1ZLvGCWMtP9BdkB1kgJJj6TNqZTsvJ5yGExeNCV6dUMfNLihVzc04RAMel6fps_7wfV_On6vH4A3J8AUa6LPZrSh_OUYpbJlErj3R87B1u6Dy7rY4Ebr-pAdfNqn8A8ZvwDguKhX</recordid><startdate>20100801</startdate><enddate>20100801</enddate><creator>Gülçin, İlhami</creator><creator>Bursal, Ercan</creator><creator>Şehitoğlu, M. Hilal</creator><creator>Bilsel, Mine</creator><creator>Gören, Ahmet C.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20100801</creationdate><title>Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey</title><author>Gülçin, İlhami ; Bursal, Ercan ; Şehitoğlu, M. Hilal ; Bilsel, Mine ; Gören, Ahmet C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c480t-68f57634d58368a4a7b9b51fff50afc357e48530617841a68f59f74ae761b1783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Anti-Infective Agents - analysis</topic><topic>Antioxidant activity</topic><topic>Antioxidants - analysis</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Flavonoids - analysis</topic><topic>Free Radical Scavengers - analysis</topic><topic>Free Radicals - metabolism</topic><topic>General pharmacology</topic><topic>LC–MS/MS</topic><topic>Limit of Detection</topic><topic>Lipid Peroxidation</topic><topic>Medical sciences</topic><topic>Metal chelating</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Phenols - analysis</topic><topic>Polyphenol content</topic><topic>Polyphenols</topic><topic>Propolis</topic><topic>Propolis - chemistry</topic><topic>Radical scavenging</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Tandem Mass Spectrometry</topic><topic>Turkey</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gülçin, İlhami</creatorcontrib><creatorcontrib>Bursal, Ercan</creatorcontrib><creatorcontrib>Şehitoğlu, M. Hilal</creatorcontrib><creatorcontrib>Bilsel, Mine</creatorcontrib><creatorcontrib>Gören, Ahmet C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Food and chemical toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gülçin, İlhami</au><au>Bursal, Ercan</au><au>Şehitoğlu, M. Hilal</au><au>Bilsel, Mine</au><au>Gören, Ahmet C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey</atitle><jtitle>Food and chemical toxicology</jtitle><addtitle>Food Chem Toxicol</addtitle><date>2010-08-01</date><risdate>2010</risdate><volume>48</volume><issue>8</issue><spage>2227</spage><epage>2238</epage><pages>2227-2238</pages><issn>0278-6915</issn><eissn>1873-6351</eissn><coden>FCTOD7</coden><abstract>Propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties attributed to the presence of polyphenols. In this study, we determined the antioxidant properties of lyophilized aqueous extract of propolis (LAEP) from Erzurum province of Turkey and correlated the values with total levels of polyphenolic compounds. In order to estimate the capacity of LAEP to act as antioxidants, we studied its 1,1-diphenyl-2-picryl-hydrazyl radicals (DPPH
), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals (ABTS
+), N,N-dimethyl-
p-phenylenediamine radicals (DMPD
+), superoxide anion radicals (
O
2
-
) and hydrogen peroxide (H
2O
2) scavenging activity, total antioxidant activity, ferric ions (Fe
3+) and cupric ions (Cu
2+) reducing ability, ferrous ions (Fe
2+) chelating activity. LAEP inhibited 93.2% lipid peroxidation of a linoleic acid emulsion at 30
μg/mL concentration. On the other hand, BHA, BHT, α-tocopherol and trolox displayed 83.3%, 82.1%, 68.1% and 81.3% inhibition of peroxidation at the same concentration, respectively. Quantitative amounts of caffeic acid, ferulic acid, syringic acid, ellagic acid, quercetin, α-tocopherol, pyrogallol,
p-hydroxybenzoic acid, vanillin,
p-coumaric acid, gallic acid and ascorbic acid were detected by high performance liquid chromatography and tandem mass spectrometry (LC–MS/MS). This study will bring an innovation for further studies with regard to the antioxidant properties of LAEP.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>20685228</pmid><doi>10.1016/j.fct.2010.05.053</doi><tpages>12</tpages></addata></record> |
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| ispartof | Food and chemical toxicology, 2010-08, Vol.48 (8), p.2227-2238 |
| issn | 0278-6915 1873-6351 |
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| subjects | Anti-Infective Agents - analysis Antioxidant activity Antioxidants - analysis Biological and medical sciences Chromatography, High Pressure Liquid Flavonoids - analysis Free Radical Scavengers - analysis Free Radicals - metabolism General pharmacology LC–MS/MS Limit of Detection Lipid Peroxidation Medical sciences Metal chelating Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Phenols - analysis Polyphenol content Polyphenols Propolis Propolis - chemistry Radical scavenging Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Turkey |
| title | Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey |
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