Assay of the Human Liver Citric Acid Cycle Probe Phenylacetylglutamine and of Phenylacetate in Plasma by Gas Chromatography-Mass Spectrometry
Phenylacetate, derived from phenylalanine, is converted in human and primate liver to phenylacetylglutamine. The latter has been used to assess the labeling pattern of liver citric acid cycle intermediates. We present gas chromatographic-mass spectrometric assays of phenylacetylglutamine, Phenylacet...
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| Veröffentlicht in: | Analytical biochemistry 1993-07, Vol.212 (1), p.277-282 |
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| creator | Yang, D.W. Beylot, M. Agarwal, K.C. Soloviev, M.V. Brunengraber, H. |
| description | Phenylacetate, derived from phenylalanine, is converted in human and primate liver to phenylacetylglutamine. The latter has been used to assess the labeling pattern of liver citric acid cycle intermediates. We present gas chromatographic-mass spectrometric assays of phenylacetylglutamine, Phenylacetate, and phenylalanine in biological fluids. The compounds are derivatized with dimethylformamide dimethyl acetal. Limits of detection are 0.1 nmol for phenylacetylglutamine and phenylacetate and 2 nmol for phenylalanine. Baseline plasma concentrations of phenylacetate and phenylacetylglutamine are 1 and 3 μM, respectively. The 24-h urinary excretions of phenylacetate and phenylacetylglutamine are about 4 μmol and 1 mmol, respectively. Ingestion of phenylalanine (in the form of aspartame) by a human is followed by sequential increases in phenylacetate and phenylacetylglutamine concentrations in plasma and urine. This assay opens the way to noninvasive probing of the 13C-labeling pattern of liver citric acid cycle intermediates in humans. |
| doi_str_mv | 10.1006/abio.1993.1323 |
| format | Article |
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The latter has been used to assess the labeling pattern of liver citric acid cycle intermediates. We present gas chromatographic-mass spectrometric assays of phenylacetylglutamine, Phenylacetate, and phenylalanine in biological fluids. The compounds are derivatized with dimethylformamide dimethyl acetal. Limits of detection are 0.1 nmol for phenylacetylglutamine and phenylacetate and 2 nmol for phenylalanine. Baseline plasma concentrations of phenylacetate and phenylacetylglutamine are 1 and 3 μM, respectively. The 24-h urinary excretions of phenylacetate and phenylacetylglutamine are about 4 μmol and 1 mmol, respectively. Ingestion of phenylalanine (in the form of aspartame) by a human is followed by sequential increases in phenylacetate and phenylacetylglutamine concentrations in plasma and urine. This assay opens the way to noninvasive probing of the 13C-labeling pattern of liver citric acid cycle intermediates in humans.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1006/abio.1993.1323</identifier><identifier>PMID: 8368503</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Adult ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Citric Acid Cycle ; Evaluation Studies as Topic ; Fundamental and applied biological sciences. Psychology ; Gas Chromatography-Mass Spectrometry - methods ; Gas Chromatography-Mass Spectrometry - statistics & numerical data ; Glutamine - analogs & derivatives ; Glutamine - analysis ; Glutamine - blood ; Humans ; Intermediary metabolites. Miscellaneous ; Liver - metabolism ; Molecular Probes ; Other biological molecules ; Phenylacetates - analysis ; Phenylacetates - blood ; Phenylalanine - analysis ; Phenylalanine - blood ; Sensitivity and Specificity</subject><ispartof>Analytical biochemistry, 1993-07, Vol.212 (1), p.277-282</ispartof><rights>1993 Academic Press</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-30c0ac00ea172d2d5fbc4584194b9a0462227c79fee3da72c80976dd4eeb505a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/abio.1993.1323$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4863612$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8368503$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, D.W.</creatorcontrib><creatorcontrib>Beylot, M.</creatorcontrib><creatorcontrib>Agarwal, K.C.</creatorcontrib><creatorcontrib>Soloviev, M.V.</creatorcontrib><creatorcontrib>Brunengraber, H.</creatorcontrib><title>Assay of the Human Liver Citric Acid Cycle Probe Phenylacetylglutamine and of Phenylacetate in Plasma by Gas Chromatography-Mass Spectrometry</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Phenylacetate, derived from phenylalanine, is converted in human and primate liver to phenylacetylglutamine. The latter has been used to assess the labeling pattern of liver citric acid cycle intermediates. We present gas chromatographic-mass spectrometric assays of phenylacetylglutamine, Phenylacetate, and phenylalanine in biological fluids. The compounds are derivatized with dimethylformamide dimethyl acetal. Limits of detection are 0.1 nmol for phenylacetylglutamine and phenylacetate and 2 nmol for phenylalanine. Baseline plasma concentrations of phenylacetate and phenylacetylglutamine are 1 and 3 μM, respectively. The 24-h urinary excretions of phenylacetate and phenylacetylglutamine are about 4 μmol and 1 mmol, respectively. Ingestion of phenylalanine (in the form of aspartame) by a human is followed by sequential increases in phenylacetate and phenylacetylglutamine concentrations in plasma and urine. This assay opens the way to noninvasive probing of the 13C-labeling pattern of liver citric acid cycle intermediates in humans.</description><subject>Adult</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Citric Acid Cycle</subject><subject>Evaluation Studies as Topic</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gas Chromatography-Mass Spectrometry - methods</subject><subject>Gas Chromatography-Mass Spectrometry - statistics & numerical data</subject><subject>Glutamine - analogs & derivatives</subject><subject>Glutamine - analysis</subject><subject>Glutamine - blood</subject><subject>Humans</subject><subject>Intermediary metabolites. Miscellaneous</subject><subject>Liver - metabolism</subject><subject>Molecular Probes</subject><subject>Other biological molecules</subject><subject>Phenylacetates - analysis</subject><subject>Phenylacetates - blood</subject><subject>Phenylalanine - analysis</subject><subject>Phenylalanine - blood</subject><subject>Sensitivity and Specificity</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE2LFDEQhoMo6-zq1ZuQg3jrsdLprxyHQXcXRlxQz6E6qd6J9MeYpBf6R_ifTTPDevKSgrxPvRQPY-8EbAVA9QlbN22FUnIrZC5fsI0AVWUgQb1kGwCQWV6p-jW7DuEXgBBFWV2xq0ZWTQlyw_7sQsCFTx2PR-J384AjP7gn8nzvoneG74yzfL-YnviDn9r0HmlcejQUl_6xnyMObiSOo11L_oUYibuRP_QYBuTtwm8x8P3RTwPG6dHj6bhkXzEE_v1EJqZvin55w1512Ad6e5k37OeXzz_2d9nh2-39fnfITLo7ZhIMoAEgFHVuc1t2rSnKphCqaBVCUeV5XptadUTSYp2bBlRdWVsQtSWUKG_Yx3PvyU-_ZwpRDy4Y6nscaZqDrkuVBDYygdszaPwUgqdOn7wb0C9agF7969W_Xv3r1X9aeH9pntuB7DN-EZ7yD5ccg8G-8zgaF56xoqlkJfKENWeMkoUnR14H42g0ZJ1PurSd3P8u-Asc4aLd</recordid><startdate>19930701</startdate><enddate>19930701</enddate><creator>Yang, D.W.</creator><creator>Beylot, M.</creator><creator>Agarwal, K.C.</creator><creator>Soloviev, M.V.</creator><creator>Brunengraber, H.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930701</creationdate><title>Assay of the Human Liver Citric Acid Cycle Probe Phenylacetylglutamine and of Phenylacetate in Plasma by Gas Chromatography-Mass Spectrometry</title><author>Yang, D.W. ; Beylot, M. ; Agarwal, K.C. ; Soloviev, M.V. ; Brunengraber, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-30c0ac00ea172d2d5fbc4584194b9a0462227c79fee3da72c80976dd4eeb505a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adult</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Citric Acid Cycle</topic><topic>Evaluation Studies as Topic</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gas Chromatography-Mass Spectrometry - methods</topic><topic>Gas Chromatography-Mass Spectrometry - statistics & numerical data</topic><topic>Glutamine - analogs & derivatives</topic><topic>Glutamine - analysis</topic><topic>Glutamine - blood</topic><topic>Humans</topic><topic>Intermediary metabolites. Miscellaneous</topic><topic>Liver - metabolism</topic><topic>Molecular Probes</topic><topic>Other biological molecules</topic><topic>Phenylacetates - analysis</topic><topic>Phenylacetates - blood</topic><topic>Phenylalanine - analysis</topic><topic>Phenylalanine - blood</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, D.W.</creatorcontrib><creatorcontrib>Beylot, M.</creatorcontrib><creatorcontrib>Agarwal, K.C.</creatorcontrib><creatorcontrib>Soloviev, M.V.</creatorcontrib><creatorcontrib>Brunengraber, H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, D.W.</au><au>Beylot, M.</au><au>Agarwal, K.C.</au><au>Soloviev, M.V.</au><au>Brunengraber, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assay of the Human Liver Citric Acid Cycle Probe Phenylacetylglutamine and of Phenylacetate in Plasma by Gas Chromatography-Mass Spectrometry</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1993-07-01</date><risdate>1993</risdate><volume>212</volume><issue>1</issue><spage>277</spage><epage>282</epage><pages>277-282</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>Phenylacetate, derived from phenylalanine, is converted in human and primate liver to phenylacetylglutamine. The latter has been used to assess the labeling pattern of liver citric acid cycle intermediates. We present gas chromatographic-mass spectrometric assays of phenylacetylglutamine, Phenylacetate, and phenylalanine in biological fluids. The compounds are derivatized with dimethylformamide dimethyl acetal. Limits of detection are 0.1 nmol for phenylacetylglutamine and phenylacetate and 2 nmol for phenylalanine. Baseline plasma concentrations of phenylacetate and phenylacetylglutamine are 1 and 3 μM, respectively. The 24-h urinary excretions of phenylacetate and phenylacetylglutamine are about 4 μmol and 1 mmol, respectively. Ingestion of phenylalanine (in the form of aspartame) by a human is followed by sequential increases in phenylacetate and phenylacetylglutamine concentrations in plasma and urine. This assay opens the way to noninvasive probing of the 13C-labeling pattern of liver citric acid cycle intermediates in humans.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8368503</pmid><doi>10.1006/abio.1993.1323</doi><tpages>6</tpages></addata></record> |
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| ispartof | Analytical biochemistry, 1993-07, Vol.212 (1), p.277-282 |
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| subjects | Adult Analytical, structural and metabolic biochemistry Biological and medical sciences Citric Acid Cycle Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology Gas Chromatography-Mass Spectrometry - methods Gas Chromatography-Mass Spectrometry - statistics & numerical data Glutamine - analogs & derivatives Glutamine - analysis Glutamine - blood Humans Intermediary metabolites. Miscellaneous Liver - metabolism Molecular Probes Other biological molecules Phenylacetates - analysis Phenylacetates - blood Phenylalanine - analysis Phenylalanine - blood Sensitivity and Specificity |
| title | Assay of the Human Liver Citric Acid Cycle Probe Phenylacetylglutamine and of Phenylacetate in Plasma by Gas Chromatography-Mass Spectrometry |
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