Detection of Gram-Negative Bacteremia by Limulus Amebocyte Lysate Assay: Evaluation in a Rat Model of Peritonitis
A spectrophotometric Limulus amebocyte lysate assay using lysis filtration and centrifugation has been developed for the detection of gram-negative bacteria in blood. The assay is directed at detection of endotoxin in viable and nonviable bacteria present in the bloodstream and not detection of free...
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| Veröffentlicht in: | The Journal of infectious diseases 1985-01, Vol.151 (1), p.148-152 |
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| container_title | The Journal of infectious diseases |
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| creator | du Moulin, Gary C. Lynch, Susan E. Hedley-Whyte, John Broitman, Selwyn A. |
| description | A spectrophotometric Limulus amebocyte lysate assay using lysis filtration and centrifugation has been developed for the detection of gram-negative bacteria in blood. The assay is directed at detection of endotoxin in viable and nonviable bacteria present in the bloodstream and not detection of free endotoxin in plasma. The assay was evaluated in a model of peritonitis in which rats were challenged with an inoculum consisting of sterilized human feces, barium sulfate, and one of eight species of bacteria. This assay was able to detect gram-negative bacteremia due to Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, Proteus mirabilis, and Klebsiella pneumoniae in the rat model when compared with sham-inoculated uninfected rats. The assay failed to detect bacteremia due to Bacteroides fragilis or Staphylococcus aureus, nor was there a significant rise in absorbance when a pellet containing sterilized feces was implanted in the rat. |
| doi_str_mv | 10.1093/infdis/151.1.148 |
| format | Article |
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The assay is directed at detection of endotoxin in viable and nonviable bacteria present in the bloodstream and not detection of free endotoxin in plasma. The assay was evaluated in a model of peritonitis in which rats were challenged with an inoculum consisting of sterilized human feces, barium sulfate, and one of eight species of bacteria. This assay was able to detect gram-negative bacteremia due to Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, Proteus mirabilis, and Klebsiella pneumoniae in the rat model when compared with sham-inoculated uninfected rats. The assay failed to detect bacteremia due to Bacteroides fragilis or Staphylococcus aureus, nor was there a significant rise in absorbance when a pellet containing sterilized feces was implanted in the rat.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/151.1.148</identifier><identifier>PMID: 3965586</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Animals ; Bacteremia ; Bacteria ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; Blood ; Capsules ; Centrifugation ; Disease Models, Animal ; Endotoxins ; Feces ; Fundamental and applied biological sciences. Psychology ; Inoculum ; Limulus Test ; Male ; Microbiology ; Original Articles ; Peritonitis ; Peritonitis - diagnosis ; Peritonitis - microbiology ; Rats ; Rats, Inbred Strains ; Sepsis - diagnosis</subject><ispartof>The Journal of infectious diseases, 1985-01, Vol.151 (1), p.148-152</ispartof><rights>Copyright 1985 The University of Chicago</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-4d3180f3ba4c7a6df50a118217a40e808df48ed236e1f2b67555c07c9676e54d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30109755$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30109755$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,4022,27922,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9261197$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3965586$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>du Moulin, Gary C.</creatorcontrib><creatorcontrib>Lynch, Susan E.</creatorcontrib><creatorcontrib>Hedley-Whyte, John</creatorcontrib><creatorcontrib>Broitman, Selwyn A.</creatorcontrib><title>Detection of Gram-Negative Bacteremia by Limulus Amebocyte Lysate Assay: Evaluation in a Rat Model of Peritonitis</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>A spectrophotometric Limulus amebocyte lysate assay using lysis filtration and centrifugation has been developed for the detection of gram-negative bacteria in blood. The assay is directed at detection of endotoxin in viable and nonviable bacteria present in the bloodstream and not detection of free endotoxin in plasma. The assay was evaluated in a model of peritonitis in which rats were challenged with an inoculum consisting of sterilized human feces, barium sulfate, and one of eight species of bacteria. This assay was able to detect gram-negative bacteremia due to Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, Proteus mirabilis, and Klebsiella pneumoniae in the rat model when compared with sham-inoculated uninfected rats. The assay failed to detect bacteremia due to Bacteroides fragilis or Staphylococcus aureus, nor was there a significant rise in absorbance when a pellet containing sterilized feces was implanted in the rat.</description><subject>Animals</subject><subject>Bacteremia</subject><subject>Bacteria</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Capsules</subject><subject>Centrifugation</subject><subject>Disease Models, Animal</subject><subject>Endotoxins</subject><subject>Feces</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Inoculum</subject><subject>Limulus Test</subject><subject>Male</subject><subject>Microbiology</subject><subject>Original Articles</subject><subject>Peritonitis</subject><subject>Peritonitis - diagnosis</subject><subject>Peritonitis - microbiology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Sepsis - diagnosis</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1TAQhSMEKpfCng2SF4hdWjt-ht3tG-kWEAKEurEmzgS55NHaTkX-PS730i7RLGZxzvms8SmK14weMFrzQz92rY-HTLKDPMI8KVZMcl0qxfjTYkVpVZXM1PXz4kWM15RSwZXeK_Z4raQ0alXcnmBCl_w0kqkj5wGG8iP-hOTvkByBSxhw8ECahWz8MPdzJOsBm8ktCclmiZDXOkZY3pPTO-hn-EvyIwHyBRK5nFrs78GfMfg0jT75-LJ41kEf8dVu7xffzk6_Hl-Um0_nH47Xm9IJJlIpWs4M7XgDwmlQbScpMGYqpkFQNNS0nTDYVlwh66pGaSmlo9rVSiuUOb1fvNtyb8J0O2NMdvDRYd_DiNMcrZY1p5LX_zUyQY1QFc1GujW6MMUYsLM3wQ8QFsuova_DbuuwuQ6bR5gcebNjz82A7UNg9_9Zf7vTITrouwCjy4B_trpSjNX6EXMd0xQeKTS_mg_PernVfUz4-0GH8MsqzbW0Fz-u7En1_ejqjCp7yf8ANkKsZQ</recordid><startdate>198501</startdate><enddate>198501</enddate><creator>du Moulin, Gary C.</creator><creator>Lynch, Susan E.</creator><creator>Hedley-Whyte, John</creator><creator>Broitman, Selwyn A.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>198501</creationdate><title>Detection of Gram-Negative Bacteremia by Limulus Amebocyte Lysate Assay: Evaluation in a Rat Model of Peritonitis</title><author>du Moulin, Gary C. ; Lynch, Susan E. ; Hedley-Whyte, John ; Broitman, Selwyn A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-4d3180f3ba4c7a6df50a118217a40e808df48ed236e1f2b67555c07c9676e54d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Bacteremia</topic><topic>Bacteria</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Capsules</topic><topic>Centrifugation</topic><topic>Disease Models, Animal</topic><topic>Endotoxins</topic><topic>Feces</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Inoculum</topic><topic>Limulus Test</topic><topic>Male</topic><topic>Microbiology</topic><topic>Original Articles</topic><topic>Peritonitis</topic><topic>Peritonitis - diagnosis</topic><topic>Peritonitis - microbiology</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Sepsis - diagnosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>du Moulin, Gary C.</creatorcontrib><creatorcontrib>Lynch, Susan E.</creatorcontrib><creatorcontrib>Hedley-Whyte, John</creatorcontrib><creatorcontrib>Broitman, Selwyn A.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>du Moulin, Gary C.</au><au>Lynch, Susan E.</au><au>Hedley-Whyte, John</au><au>Broitman, Selwyn A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Gram-Negative Bacteremia by Limulus Amebocyte Lysate Assay: Evaluation in a Rat Model of Peritonitis</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1985-01</date><risdate>1985</risdate><volume>151</volume><issue>1</issue><spage>148</spage><epage>152</epage><pages>148-152</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>A spectrophotometric Limulus amebocyte lysate assay using lysis filtration and centrifugation has been developed for the detection of gram-negative bacteria in blood. The assay is directed at detection of endotoxin in viable and nonviable bacteria present in the bloodstream and not detection of free endotoxin in plasma. The assay was evaluated in a model of peritonitis in which rats were challenged with an inoculum consisting of sterilized human feces, barium sulfate, and one of eight species of bacteria. This assay was able to detect gram-negative bacteremia due to Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, Proteus mirabilis, and Klebsiella pneumoniae in the rat model when compared with sham-inoculated uninfected rats. The assay failed to detect bacteremia due to Bacteroides fragilis or Staphylococcus aureus, nor was there a significant rise in absorbance when a pellet containing sterilized feces was implanted in the rat.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>3965586</pmid><doi>10.1093/infdis/151.1.148</doi><tpages>5</tpages></addata></record> |
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| ispartof | The Journal of infectious diseases, 1985-01, Vol.151 (1), p.148-152 |
| issn | 0022-1899 1537-6613 |
| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing |
| subjects | Animals Bacteremia Bacteria Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Blood Capsules Centrifugation Disease Models, Animal Endotoxins Feces Fundamental and applied biological sciences. Psychology Inoculum Limulus Test Male Microbiology Original Articles Peritonitis Peritonitis - diagnosis Peritonitis - microbiology Rats Rats, Inbred Strains Sepsis - diagnosis |
| title | Detection of Gram-Negative Bacteremia by Limulus Amebocyte Lysate Assay: Evaluation in a Rat Model of Peritonitis |
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