Restriction‐enzyme cleavage of DNA modified by platinum(II) complexes
The effect of binding of cis‐diamminedichloroplatinum(II), its trans isomer and diethylenetriami‐nechloroplatinum(II) chloride to DNA on the splicing effectiveness of BamHI, EcoRI and SalI restriction endonucleases has been determined by means of gel electrophoresis. All three platinum complexes inh...
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| Veröffentlicht in: | European journal of biochemistry 1993-08, Vol.216 (1), p.183-187 |
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| container_title | European journal of biochemistry |
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| creator | BRABEC, Viktor BALCAROVA, Zdenka |
| description | The effect of binding of cis‐diamminedichloroplatinum(II), its trans isomer and diethylenetriami‐nechloroplatinum(II) chloride to DNA on the splicing effectiveness of BamHI, EcoRI and SalI restriction endonucleases has been determined by means of gel electrophoresis. All three platinum complexes inhibit the cleavage of linearized plasmid DNA. In addition, the three platinum complexes bound to DNA constitute a barrier across which the linear diffusion of EcoRI on DNA is difficult. We interprete these findings to mean that the splicing effectiveness of restriction enzymes is influenced by bifunctional and monofunctional DNA adducts of platinum via both steric interference and DNA conformational distortions. Whereas the platinum adducts in the restriction sites or in their very close proximity inhibit the cleavage, the lesions occurring a greater distance from the restriction site can slow down the process of the localization of recognition sequences. |
| doi_str_mv | 10.1111/j.1432-1033.1993.tb18131.x |
| format | Article |
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All three platinum complexes inhibit the cleavage of linearized plasmid DNA. In addition, the three platinum complexes bound to DNA constitute a barrier across which the linear diffusion of EcoRI on DNA is difficult. We interprete these findings to mean that the splicing effectiveness of restriction enzymes is influenced by bifunctional and monofunctional DNA adducts of platinum via both steric interference and DNA conformational distortions. Whereas the platinum adducts in the restriction sites or in their very close proximity inhibit the cleavage, the lesions occurring a greater distance from the restriction site can slow down the process of the localization of recognition sequences.</description><identifier>ISSN: 0014-2956</identifier><identifier>EISSN: 1432-1033</identifier><identifier>DOI: 10.1111/j.1432-1033.1993.tb18131.x</identifier><identifier>PMID: 8365404</identifier><identifier>CODEN: EJBCAI</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Antineoplastic agents ; Binding Sites ; Biological and medical sciences ; Cisplatin - pharmacology ; Deoxyribonuclease BamHI - metabolism ; Deoxyribonuclease EcoRI - metabolism ; Deoxyribonucleases, Type II Site-Specific - metabolism ; DNA - metabolism ; Electrophoresis, Agar Gel ; General aspects ; Medical sciences ; Organoplatinum Compounds - pharmacology ; Pharmacology. Drug treatments ; Plasmids - drug effects ; Stereoisomerism</subject><ispartof>European journal of biochemistry, 1993-08, Vol.216 (1), p.183-187</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4513-d8e21838ca359e5fb8abfbdd53a5a307def30d030b4b269bddb3e94b71bf0b093</citedby><cites>FETCH-LOGICAL-c4513-d8e21838ca359e5fb8abfbdd53a5a307def30d030b4b269bddb3e94b71bf0b093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4899998$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8365404$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BRABEC, Viktor</creatorcontrib><creatorcontrib>BALCAROVA, Zdenka</creatorcontrib><title>Restriction‐enzyme cleavage of DNA modified by platinum(II) complexes</title><title>European journal of biochemistry</title><addtitle>Eur J Biochem</addtitle><description>The effect of binding of cis‐diamminedichloroplatinum(II), its trans isomer and diethylenetriami‐nechloroplatinum(II) chloride to DNA on the splicing effectiveness of BamHI, EcoRI and SalI restriction endonucleases has been determined by means of gel electrophoresis. All three platinum complexes inhibit the cleavage of linearized plasmid DNA. In addition, the three platinum complexes bound to DNA constitute a barrier across which the linear diffusion of EcoRI on DNA is difficult. We interprete these findings to mean that the splicing effectiveness of restriction enzymes is influenced by bifunctional and monofunctional DNA adducts of platinum via both steric interference and DNA conformational distortions. Whereas the platinum adducts in the restriction sites or in their very close proximity inhibit the cleavage, the lesions occurring a greater distance from the restriction site can slow down the process of the localization of recognition sequences.</description><subject>Antineoplastic agents</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Cisplatin - pharmacology</subject><subject>Deoxyribonuclease BamHI - metabolism</subject><subject>Deoxyribonuclease EcoRI - metabolism</subject><subject>Deoxyribonucleases, Type II Site-Specific - metabolism</subject><subject>DNA - metabolism</subject><subject>Electrophoresis, Agar Gel</subject><subject>General aspects</subject><subject>Medical sciences</subject><subject>Organoplatinum Compounds - pharmacology</subject><subject>Pharmacology. Drug treatments</subject><subject>Plasmids - drug effects</subject><subject>Stereoisomerism</subject><issn>0014-2956</issn><issn>1432-1033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkMtKAzEUhoMoWi-PIAwioosZk8lcEjdSL60FUfCyDknmRFLmUiettq58BJ_RJzGlQ_eezVn837nwIXREcER8nY8jktA4JJjSiHBOo6kijFASzTdQbx1toh7GJAljnmY7aNe5McY441m-jbYZzdIEJz00fAI3ba2e2qb-_f6B-mtRQaBLkB_yDYLGBDcP_aBqCmssFIFaBJNSTm09q05Ho7NAN9WkhDm4fbRlZOngoOt76HVw-3J9F94_DkfX_ftQJymhYcEgJowyLWnKITWKSWVUUaRUppLivABDcYEpVomKM-4TRYEnKifKYIU53UMnq72Ttnmf-d9FZZ2GspQ1NDMn8pTHGeO5By9WoG4b51owYtLaSrYLQbBYWhRjsVQllqrE0qLoLIq5Hz7srsxUBcV6tNPm8-Mul07L0rSy1tatsYRxX8xjlyvs05aw-McDYnB79ew90T-u6JAc</recordid><startdate>19930815</startdate><enddate>19930815</enddate><creator>BRABEC, Viktor</creator><creator>BALCAROVA, Zdenka</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930815</creationdate><title>Restriction‐enzyme cleavage of DNA modified by platinum(II) complexes</title><author>BRABEC, Viktor ; BALCAROVA, Zdenka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4513-d8e21838ca359e5fb8abfbdd53a5a307def30d030b4b269bddb3e94b71bf0b093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Antineoplastic agents</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Cisplatin - pharmacology</topic><topic>Deoxyribonuclease BamHI - metabolism</topic><topic>Deoxyribonuclease EcoRI - metabolism</topic><topic>Deoxyribonucleases, Type II Site-Specific - metabolism</topic><topic>DNA - metabolism</topic><topic>Electrophoresis, Agar Gel</topic><topic>General aspects</topic><topic>Medical sciences</topic><topic>Organoplatinum Compounds - pharmacology</topic><topic>Pharmacology. Drug treatments</topic><topic>Plasmids - drug effects</topic><topic>Stereoisomerism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BRABEC, Viktor</creatorcontrib><creatorcontrib>BALCAROVA, Zdenka</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BRABEC, Viktor</au><au>BALCAROVA, Zdenka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Restriction‐enzyme cleavage of DNA modified by platinum(II) complexes</atitle><jtitle>European journal of biochemistry</jtitle><addtitle>Eur J Biochem</addtitle><date>1993-08-15</date><risdate>1993</risdate><volume>216</volume><issue>1</issue><spage>183</spage><epage>187</epage><pages>183-187</pages><issn>0014-2956</issn><eissn>1432-1033</eissn><coden>EJBCAI</coden><abstract>The effect of binding of cis‐diamminedichloroplatinum(II), its trans isomer and diethylenetriami‐nechloroplatinum(II) chloride to DNA on the splicing effectiveness of BamHI, EcoRI and SalI restriction endonucleases has been determined by means of gel electrophoresis. All three platinum complexes inhibit the cleavage of linearized plasmid DNA. In addition, the three platinum complexes bound to DNA constitute a barrier across which the linear diffusion of EcoRI on DNA is difficult. We interprete these findings to mean that the splicing effectiveness of restriction enzymes is influenced by bifunctional and monofunctional DNA adducts of platinum via both steric interference and DNA conformational distortions. Whereas the platinum adducts in the restriction sites or in their very close proximity inhibit the cleavage, the lesions occurring a greater distance from the restriction site can slow down the process of the localization of recognition sequences.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8365404</pmid><doi>10.1111/j.1432-1033.1993.tb18131.x</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | European journal of biochemistry, 1993-08, Vol.216 (1), p.183-187 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Antineoplastic agents Binding Sites Biological and medical sciences Cisplatin - pharmacology Deoxyribonuclease BamHI - metabolism Deoxyribonuclease EcoRI - metabolism Deoxyribonucleases, Type II Site-Specific - metabolism DNA - metabolism Electrophoresis, Agar Gel General aspects Medical sciences Organoplatinum Compounds - pharmacology Pharmacology. Drug treatments Plasmids - drug effects Stereoisomerism |
| title | Restriction‐enzyme cleavage of DNA modified by platinum(II) complexes |
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