Vesicular transport of myelin proteolipid and cerebroside sulfates to the myelin membrane
The possibility that cerebroside sulfates and myelin proteolipid (PLP) could be simultaneously located in transport vesicles destined to be assembled in myelin was investigated in the brain of 20 day old rats. The brain was homogenized and fractionated according to Burkart et al. (J Biol Chem 257:31...
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| Veröffentlicht in: | Journal of neuroscience research 1993-07, Vol.35 (4), p.402-408 |
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| container_title | Journal of neuroscience research |
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| creator | Brown, M. C. Moreno, M. Besio Bongarzone, E. R. Cohen, P. D. Soto, E. F. Pasquini, J. M. |
| description | The possibility that cerebroside sulfates and myelin proteolipid (PLP) could be simultaneously located in transport vesicles destined to be assembled in myelin was investigated in the brain of 20 day old rats. The brain was homogenized and fractionated according to Burkart et al. (J Biol Chem 257:3151–3156, 1982) to obtain a microsomal fraction that was further subfractionated in a linear sucrose density gradient following the procedure of Siegrist et al. (J Neurochem 33:497–504, 1979) to obtain a vesicular fraction which has been shown to transport cerebroside sulfates (Burkart et al., as above). This fraction was associated with acid hydrolase activity and had a lipid composition different from that of myelin and microsomal fractions. Studied by slab gel electrophoresis, dot blot, and Western blot analysis, using a highly specific anti‐PLP antibody, it was found to contain myelin PLP. In view of previous findings of several laboratories including our own, the presence of myelin proteolipid in a vesicular fraction which is related to the transport of cerebroside sulfates gives further support to the hypothesis that the delivery of both constituents to the myelin membrane could be associated. © 1993 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jnr.490350407 |
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C. ; Moreno, M. Besio ; Bongarzone, E. R. ; Cohen, P. D. ; Soto, E. F. ; Pasquini, J. M.</creator><creatorcontrib>Brown, M. C. ; Moreno, M. Besio ; Bongarzone, E. R. ; Cohen, P. D. ; Soto, E. F. ; Pasquini, J. M.</creatorcontrib><description>The possibility that cerebroside sulfates and myelin proteolipid (PLP) could be simultaneously located in transport vesicles destined to be assembled in myelin was investigated in the brain of 20 day old rats. The brain was homogenized and fractionated according to Burkart et al. (J Biol Chem 257:3151–3156, 1982) to obtain a microsomal fraction that was further subfractionated in a linear sucrose density gradient following the procedure of Siegrist et al. (J Neurochem 33:497–504, 1979) to obtain a vesicular fraction which has been shown to transport cerebroside sulfates (Burkart et al., as above). This fraction was associated with acid hydrolase activity and had a lipid composition different from that of myelin and microsomal fractions. Studied by slab gel electrophoresis, dot blot, and Western blot analysis, using a highly specific anti‐PLP antibody, it was found to contain myelin PLP. In view of previous findings of several laboratories including our own, the presence of myelin proteolipid in a vesicular fraction which is related to the transport of cerebroside sulfates gives further support to the hypothesis that the delivery of both constituents to the myelin membrane could be associated. © 1993 Wiley‐Liss, Inc.</description><identifier>ISSN: 0360-4012</identifier><identifier>EISSN: 1097-4547</identifier><identifier>DOI: 10.1002/jnr.490350407</identifier><identifier>PMID: 8360948</identifier><identifier>CODEN: JNREDK</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biochemistry and metabolism ; Biological and medical sciences ; Blotting, Western ; Brain - ultrastructure ; Brain Chemistry - physiology ; Central nervous system ; Electrophoresis, Polyacrylamide Gel ; Female ; Fundamental and applied biological sciences. Psychology ; Immunoblotting ; Male ; Microsomes - enzymology ; Microsomes - metabolism ; myelin assembly ; myelin PLP ; Myelin Sheath - enzymology ; Myelin Sheath - metabolism ; Oligodendroglia - metabolism ; Proteolipids - metabolism ; Rats ; Rats, Wistar ; Subcellular Fractions - enzymology ; Subcellular Fractions - metabolism ; sulfatides ; Sulfoglycosphingolipids - metabolism ; Vertebrates: nervous system and sense organs ; vesicular transport</subject><ispartof>Journal of neuroscience research, 1993-07, Vol.35 (4), p.402-408</ispartof><rights>Copyright © 1993 Wiley‐Liss, Inc.</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5007-f8ced10cb5af111260fceb3d4fc0febefdcf2d561e3b34b6b4f75222ec13fe1f3</citedby><cites>FETCH-LOGICAL-c5007-f8ced10cb5af111260fceb3d4fc0febefdcf2d561e3b34b6b4f75222ec13fe1f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjnr.490350407$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjnr.490350407$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4791100$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8360948$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brown, M. C.</creatorcontrib><creatorcontrib>Moreno, M. Besio</creatorcontrib><creatorcontrib>Bongarzone, E. R.</creatorcontrib><creatorcontrib>Cohen, P. D.</creatorcontrib><creatorcontrib>Soto, E. F.</creatorcontrib><creatorcontrib>Pasquini, J. M.</creatorcontrib><title>Vesicular transport of myelin proteolipid and cerebroside sulfates to the myelin membrane</title><title>Journal of neuroscience research</title><addtitle>J. Neurosci. Res</addtitle><description>The possibility that cerebroside sulfates and myelin proteolipid (PLP) could be simultaneously located in transport vesicles destined to be assembled in myelin was investigated in the brain of 20 day old rats. The brain was homogenized and fractionated according to Burkart et al. (J Biol Chem 257:3151–3156, 1982) to obtain a microsomal fraction that was further subfractionated in a linear sucrose density gradient following the procedure of Siegrist et al. (J Neurochem 33:497–504, 1979) to obtain a vesicular fraction which has been shown to transport cerebroside sulfates (Burkart et al., as above). This fraction was associated with acid hydrolase activity and had a lipid composition different from that of myelin and microsomal fractions. Studied by slab gel electrophoresis, dot blot, and Western blot analysis, using a highly specific anti‐PLP antibody, it was found to contain myelin PLP. In view of previous findings of several laboratories including our own, the presence of myelin proteolipid in a vesicular fraction which is related to the transport of cerebroside sulfates gives further support to the hypothesis that the delivery of both constituents to the myelin membrane could be associated. © 1993 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Brain - ultrastructure</subject><subject>Brain Chemistry - physiology</subject><subject>Central nervous system</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunoblotting</subject><subject>Male</subject><subject>Microsomes - enzymology</subject><subject>Microsomes - metabolism</subject><subject>myelin assembly</subject><subject>myelin PLP</subject><subject>Myelin Sheath - enzymology</subject><subject>Myelin Sheath - metabolism</subject><subject>Oligodendroglia - metabolism</subject><subject>Proteolipids - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Subcellular Fractions - enzymology</subject><subject>Subcellular Fractions - metabolism</subject><subject>sulfatides</subject><subject>Sulfoglycosphingolipids - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>vesicular transport</subject><issn>0360-4012</issn><issn>1097-4547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1v1DAQxS0EKkvhyBHJB9Rbyvg7OaKKFkopH4JFnCzHGQuXZLPYiej-9xhtWPUEpzm837x58wh5yuCUAfAXN5t0KhsQCiSYe2TFoDGVVNLcJysQGioJjD8kj3K-AYCmUeKIHNVFaGS9It_WmKOfe5folNwmb8c00THQYYd93NBtGicc-7iNHXWbjnpM2KYxxw5pnvvgJsx0Gun0Hf-uDDi0xQkfkwfB9RmfLPOYfDl_9fnsdXX1_uLN2curyisAU4XaY8fAt8oFxhjXEDy2opPBQ8AWQ-cD75RmKFohW93KYBTnHD0TAVkQx-Rk71uy_pwxT3aI2WPflwzjnK1RDdfK6P-CTGve1DUvYLUHffk0Jwx2m-Lg0s4ysH86t6Vze-i88M8W47kdsDvQS8lFf77oLnvXh9KOj_mASdOw4lows8d-xR53_75pL68_3Q2wBI55wtvDpks_rDbCKPv1-sK-Mx8u1x_V2r4VvwFwS6uU</recordid><startdate>19930701</startdate><enddate>19930701</enddate><creator>Brown, M. C.</creator><creator>Moreno, M. Besio</creator><creator>Bongarzone, E. R.</creator><creator>Cohen, P. D.</creator><creator>Soto, E. F.</creator><creator>Pasquini, J. M.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19930701</creationdate><title>Vesicular transport of myelin proteolipid and cerebroside sulfates to the myelin membrane</title><author>Brown, M. C. ; Moreno, M. Besio ; Bongarzone, E. R. ; Cohen, P. D. ; Soto, E. F. ; Pasquini, J. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5007-f8ced10cb5af111260fceb3d4fc0febefdcf2d561e3b34b6b4f75222ec13fe1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Brain - ultrastructure</topic><topic>Brain Chemistry - physiology</topic><topic>Central nervous system</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunoblotting</topic><topic>Male</topic><topic>Microsomes - enzymology</topic><topic>Microsomes - metabolism</topic><topic>myelin assembly</topic><topic>myelin PLP</topic><topic>Myelin Sheath - enzymology</topic><topic>Myelin Sheath - metabolism</topic><topic>Oligodendroglia - metabolism</topic><topic>Proteolipids - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Subcellular Fractions - enzymology</topic><topic>Subcellular Fractions - metabolism</topic><topic>sulfatides</topic><topic>Sulfoglycosphingolipids - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>vesicular transport</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brown, M. C.</creatorcontrib><creatorcontrib>Moreno, M. Besio</creatorcontrib><creatorcontrib>Bongarzone, E. R.</creatorcontrib><creatorcontrib>Cohen, P. D.</creatorcontrib><creatorcontrib>Soto, E. 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Neurosci. Res</addtitle><date>1993-07-01</date><risdate>1993</risdate><volume>35</volume><issue>4</issue><spage>402</spage><epage>408</epage><pages>402-408</pages><issn>0360-4012</issn><eissn>1097-4547</eissn><coden>JNREDK</coden><abstract>The possibility that cerebroside sulfates and myelin proteolipid (PLP) could be simultaneously located in transport vesicles destined to be assembled in myelin was investigated in the brain of 20 day old rats. The brain was homogenized and fractionated according to Burkart et al. (J Biol Chem 257:3151–3156, 1982) to obtain a microsomal fraction that was further subfractionated in a linear sucrose density gradient following the procedure of Siegrist et al. (J Neurochem 33:497–504, 1979) to obtain a vesicular fraction which has been shown to transport cerebroside sulfates (Burkart et al., as above). This fraction was associated with acid hydrolase activity and had a lipid composition different from that of myelin and microsomal fractions. Studied by slab gel electrophoresis, dot blot, and Western blot analysis, using a highly specific anti‐PLP antibody, it was found to contain myelin PLP. In view of previous findings of several laboratories including our own, the presence of myelin proteolipid in a vesicular fraction which is related to the transport of cerebroside sulfates gives further support to the hypothesis that the delivery of both constituents to the myelin membrane could be associated. © 1993 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>8360948</pmid><doi>10.1002/jnr.490350407</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of neuroscience research, 1993-07, Vol.35 (4), p.402-408 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Biochemistry and metabolism Biological and medical sciences Blotting, Western Brain - ultrastructure Brain Chemistry - physiology Central nervous system Electrophoresis, Polyacrylamide Gel Female Fundamental and applied biological sciences. Psychology Immunoblotting Male Microsomes - enzymology Microsomes - metabolism myelin assembly myelin PLP Myelin Sheath - enzymology Myelin Sheath - metabolism Oligodendroglia - metabolism Proteolipids - metabolism Rats Rats, Wistar Subcellular Fractions - enzymology Subcellular Fractions - metabolism sulfatides Sulfoglycosphingolipids - metabolism Vertebrates: nervous system and sense organs vesicular transport |
| title | Vesicular transport of myelin proteolipid and cerebroside sulfates to the myelin membrane |
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