Adenylate cyclase-the more membrane associated, the less radiosensitive
A dose dependent but not parallel decreases were observed both in SH content and catalytic activity of "free" catalytic subunit after irradiation (0-3200 Gy), while SH groups of membrane-associated adenylate cyclase were insensitive (under 3200 Gy). An initial "radioactivation" o...
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| Veröffentlicht in: | Journal of radiation research 1993, Vol.34 (2), p.141-147 |
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| container_title | Journal of radiation research |
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| creator | Takáts, A Binh, V H Offenmüller, K Bodó, K Bertók, L Köteles, G J |
| description | A dose dependent but not parallel decreases were observed both in SH content and catalytic activity of "free" catalytic subunit after irradiation (0-3200 Gy), while SH groups of membrane-associated adenylate cyclase were insensitive (under 3200 Gy). An initial "radioactivation" of membrane-associated enzyme was found under 800 Gy, then an inhibition above 1600 Gy. The SH alkylating agent, N-ethylmaleimide resulted in a complete inactivation, both of membrane associated form of adenylate cyclase and "free" catalytic subunit with similar inactivation profiles. These data indicate that in the radiosensitivity or "radioprotection" of adenylate cyclase, its membrane association/integration might play a more important role than the SH groups themselves. |
| doi_str_mv | 10.1269/jrr.34.141 |
| format | Article |
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An initial "radioactivation" of membrane-associated enzyme was found under 800 Gy, then an inhibition above 1600 Gy. The SH alkylating agent, N-ethylmaleimide resulted in a complete inactivation, both of membrane associated form of adenylate cyclase and "free" catalytic subunit with similar inactivation profiles. These data indicate that in the radiosensitivity or "radioprotection" of adenylate cyclase, its membrane association/integration might play a more important role than the SH groups themselves.</description><identifier>ISSN: 0449-3060</identifier><identifier>EISSN: 1349-9157</identifier><identifier>DOI: 10.1269/jrr.34.141</identifier><identifier>PMID: 8360856</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adenylyl Cyclases - radiation effects ; Animals ; Brain - enzymology ; Brain - ultrastructure ; Cell Membrane - enzymology ; Chick Embryo ; In Vitro Techniques ; Radiation Tolerance</subject><ispartof>Journal of radiation research, 1993, Vol.34 (2), p.141-147</ispartof><rights>Copyright Japan Science and Technology Agency 1993</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8360856$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takáts, A</creatorcontrib><creatorcontrib>Binh, V H</creatorcontrib><creatorcontrib>Offenmüller, K</creatorcontrib><creatorcontrib>Bodó, K</creatorcontrib><creatorcontrib>Bertók, L</creatorcontrib><creatorcontrib>Köteles, G J</creatorcontrib><title>Adenylate cyclase-the more membrane associated, the less radiosensitive</title><title>Journal of radiation research</title><addtitle>J Radiat Res</addtitle><description>A dose dependent but not parallel decreases were observed both in SH content and catalytic activity of "free" catalytic subunit after irradiation (0-3200 Gy), while SH groups of membrane-associated adenylate cyclase were insensitive (under 3200 Gy). An initial "radioactivation" of membrane-associated enzyme was found under 800 Gy, then an inhibition above 1600 Gy. The SH alkylating agent, N-ethylmaleimide resulted in a complete inactivation, both of membrane associated form of adenylate cyclase and "free" catalytic subunit with similar inactivation profiles. These data indicate that in the radiosensitivity or "radioprotection" of adenylate cyclase, its membrane association/integration might play a more important role than the SH groups themselves.</description><subject>Adenylyl Cyclases - radiation effects</subject><subject>Animals</subject><subject>Brain - enzymology</subject><subject>Brain - ultrastructure</subject><subject>Cell Membrane - enzymology</subject><subject>Chick Embryo</subject><subject>In Vitro Techniques</subject><subject>Radiation Tolerance</subject><issn>0449-3060</issn><issn>1349-9157</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctLw0AQxhdRaq1evAsBwYOYOvvM7rEUrULBi56XTTLBlDzqbiL0v3dLiwcvXr4ZmN98zIOQawpzypR53Hg_52JOBT0hU8qFSQ2V2SmZgog5BwXn5CKEDQDLQMKETDRXoKWaktWixG7XuAGTYlc0LmA6fGLS9j4Ktrl3HSYuhL6oI1M-JPtqgyEk3pV1H7AL9VB_4yU5q1wT8OoYZ-Tj-el9-ZKu31avy8U6LSTwIVUsy5lyJRjDKqlLJStVcoWaZxAlL5yqQCNqLYFpTQ0KTTUYl7scmEI-I3cH363vv0YMg23rUGDTxDn7MdhMGiZBin9BqjLOVMYiePsH3PSj7-ISlgohtBEaeKTuD1Th-xA8Vnbr69b5naVg90-w8QmWi9hDI3xztBzzFstf9Hh1_gNEJoDX</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>Takáts, A</creator><creator>Binh, V H</creator><creator>Offenmüller, K</creator><creator>Bodó, K</creator><creator>Bertók, L</creator><creator>Köteles, G J</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>1993</creationdate><title>Adenylate cyclase-the more membrane associated, the less radiosensitive</title><author>Takáts, A ; Binh, V H ; Offenmüller, K ; Bodó, K ; Bertók, L ; Köteles, G J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-627b26ad0992f58d65f6d36e8370e83bca6f08ee885028819e481809abab026e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adenylyl Cyclases - radiation effects</topic><topic>Animals</topic><topic>Brain - enzymology</topic><topic>Brain - ultrastructure</topic><topic>Cell Membrane - enzymology</topic><topic>Chick Embryo</topic><topic>In Vitro Techniques</topic><topic>Radiation Tolerance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takáts, A</creatorcontrib><creatorcontrib>Binh, V H</creatorcontrib><creatorcontrib>Offenmüller, K</creatorcontrib><creatorcontrib>Bodó, K</creatorcontrib><creatorcontrib>Bertók, L</creatorcontrib><creatorcontrib>Köteles, G J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of radiation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takáts, A</au><au>Binh, V H</au><au>Offenmüller, K</au><au>Bodó, K</au><au>Bertók, L</au><au>Köteles, G J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adenylate cyclase-the more membrane associated, the less radiosensitive</atitle><jtitle>Journal of radiation research</jtitle><addtitle>J Radiat Res</addtitle><date>1993</date><risdate>1993</risdate><volume>34</volume><issue>2</issue><spage>141</spage><epage>147</epage><pages>141-147</pages><issn>0449-3060</issn><eissn>1349-9157</eissn><abstract>A dose dependent but not parallel decreases were observed both in SH content and catalytic activity of "free" catalytic subunit after irradiation (0-3200 Gy), while SH groups of membrane-associated adenylate cyclase were insensitive (under 3200 Gy). An initial "radioactivation" of membrane-associated enzyme was found under 800 Gy, then an inhibition above 1600 Gy. The SH alkylating agent, N-ethylmaleimide resulted in a complete inactivation, both of membrane associated form of adenylate cyclase and "free" catalytic subunit with similar inactivation profiles. These data indicate that in the radiosensitivity or "radioprotection" of adenylate cyclase, its membrane association/integration might play a more important role than the SH groups themselves.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>8360856</pmid><doi>10.1269/jrr.34.141</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of radiation research, 1993, Vol.34 (2), p.141-147 |
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| language | eng |
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| source | Oxford Journals Open Access Collection; MEDLINE; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Adenylyl Cyclases - radiation effects Animals Brain - enzymology Brain - ultrastructure Cell Membrane - enzymology Chick Embryo In Vitro Techniques Radiation Tolerance |
| title | Adenylate cyclase-the more membrane associated, the less radiosensitive |
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