Role of Thrombospondin in the Adhesion of Human Endothelial Cells in Primary Culture
The role of thrombospondin on the adhesion of endothelial cells in primary culture was studied using a serum-free defined medium or thrombospondin-depleted fetal bovine serum. Under these conditions, only 6% of the cells adhered to gelatin-coated dishes, whereas cells adhering to gelatin in the pres...
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| Veröffentlicht in: | In Vitro Cellular & Developmental Biology - Animal 1993-07, Vol.29A (7), p.585-591 |
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| container_title | In Vitro Cellular & Developmental Biology - Animal |
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| creator | Veronica Morandi Françoise Fauvel-Lafeve Chantal Legrand Legrand, Yves J. |
| description | The role of thrombospondin on the adhesion of endothelial cells in primary culture was studied using a serum-free defined medium or thrombospondin-depleted fetal bovine serum. Under these conditions, only 6% of the cells adhered to gelatin-coated dishes, whereas cells adhering to gelatin in the presence of normal fetal bovine serum were considered as 100% adhesion. The percentage of cells attached to fibronectin or thrombospondin-coated dishes in thrombospondin-depleted serum was 66 and 32%, respectively. The addition of purified platelet thrombospondin to thrombospondin-depleted serum increased the adhesion of endothelial cells to gelatin and to thrombospondin, up to 32 and 59%, respectively, and restored the attachment to fibronectin to the same extent as that observed in the presence of normal serum. In contrast to the attachment, the spreading of the adhering cells was not further influenced by the addition of soluble thrombospondin. Subcultured cells did not require any protein for adhering to gelatin substrata. These observations indicate that thrombospondin plays a major role in the adhesion of endothelial cells in primary culture. |
| doi_str_mv | 10.1007/BF02634152 |
| format | Article |
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Under these conditions, only 6% of the cells adhered to gelatin-coated dishes, whereas cells adhering to gelatin in the presence of normal fetal bovine serum were considered as 100% adhesion. The percentage of cells attached to fibronectin or thrombospondin-coated dishes in thrombospondin-depleted serum was 66 and 32%, respectively. The addition of purified platelet thrombospondin to thrombospondin-depleted serum increased the adhesion of endothelial cells to gelatin and to thrombospondin, up to 32 and 59%, respectively, and restored the attachment to fibronectin to the same extent as that observed in the presence of normal serum. In contrast to the attachment, the spreading of the adhering cells was not further influenced by the addition of soluble thrombospondin. Subcultured cells did not require any protein for adhering to gelatin substrata. These observations indicate that thrombospondin plays a major role in the adhesion of endothelial cells in primary culture.</description><identifier>ISSN: 1071-2690</identifier><identifier>ISSN: 0883-8364</identifier><identifier>EISSN: 1543-706X</identifier><identifier>EISSN: 2327-431X</identifier><identifier>DOI: 10.1007/BF02634152</identifier><identifier>PMID: 8354667</identifier><identifier>CODEN: ICDBEO</identifier><language>eng</language><publisher>Largo, MD: Tissue Culture Association, Inc</publisher><subject>Biological and medical sciences ; Cell adhesion ; Cell Adhesion - physiology ; Cell interactions, adhesion ; Cell lines ; Cells ; Cells, Cultured ; Cellular Models ; Culture Media, Conditioned - pharmacology ; Culture Media, Serum-Free - pharmacology ; Cultured cells ; Endothelial cells ; Endothelium, Vascular - cytology ; Endothelium, Vascular - physiology ; Enzyme-Linked Immunosorbent Assay ; Fibronectins - physiology ; Fibrosis ; Fundamental and applied biological sciences. Psychology ; Gelatins ; Humans ; Membrane Glycoproteins - physiology ; Molecular and cellular biology ; Neuroglia ; Platelets ; Thrombospondins ; Umbilical Veins - cytology</subject><ispartof>In Vitro Cellular & Developmental Biology - Animal, 1993-07, Vol.29A (7), p.585-591</ispartof><rights>Copyright 1993 Tissue Culture Association</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-d0f5bb42aaa88c0e73cc860d624ce285f4735c98048702b911ace3a6c1f4b1653</citedby><cites>FETCH-LOGICAL-c332t-d0f5bb42aaa88c0e73cc860d624ce285f4735c98048702b911ace3a6c1f4b1653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4294082$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4294082$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3748201$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8354667$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Veronica Morandi</creatorcontrib><creatorcontrib>Françoise Fauvel-Lafeve</creatorcontrib><creatorcontrib>Chantal Legrand</creatorcontrib><creatorcontrib>Legrand, Yves J.</creatorcontrib><title>Role of Thrombospondin in the Adhesion of Human Endothelial Cells in Primary Culture</title><title>In Vitro Cellular & Developmental Biology - Animal</title><addtitle>In Vitro Cell Dev Biol Anim</addtitle><description>The role of thrombospondin on the adhesion of endothelial cells in primary culture was studied using a serum-free defined medium or thrombospondin-depleted fetal bovine serum. Under these conditions, only 6% of the cells adhered to gelatin-coated dishes, whereas cells adhering to gelatin in the presence of normal fetal bovine serum were considered as 100% adhesion. The percentage of cells attached to fibronectin or thrombospondin-coated dishes in thrombospondin-depleted serum was 66 and 32%, respectively. The addition of purified platelet thrombospondin to thrombospondin-depleted serum increased the adhesion of endothelial cells to gelatin and to thrombospondin, up to 32 and 59%, respectively, and restored the attachment to fibronectin to the same extent as that observed in the presence of normal serum. In contrast to the attachment, the spreading of the adhering cells was not further influenced by the addition of soluble thrombospondin. Subcultured cells did not require any protein for adhering to gelatin substrata. These observations indicate that thrombospondin plays a major role in the adhesion of endothelial cells in primary culture.</description><subject>Biological and medical sciences</subject><subject>Cell adhesion</subject><subject>Cell Adhesion - physiology</subject><subject>Cell interactions, adhesion</subject><subject>Cell lines</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>Cellular Models</subject><subject>Culture Media, Conditioned - pharmacology</subject><subject>Culture Media, Serum-Free - pharmacology</subject><subject>Cultured cells</subject><subject>Endothelial cells</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - physiology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fibronectins - physiology</subject><subject>Fibrosis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gelatins</subject><subject>Humans</subject><subject>Membrane Glycoproteins - physiology</subject><subject>Molecular and cellular biology</subject><subject>Neuroglia</subject><subject>Platelets</subject><subject>Thrombospondins</subject><subject>Umbilical Veins - cytology</subject><issn>1071-2690</issn><issn>0883-8364</issn><issn>1543-706X</issn><issn>2327-431X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkN1LwzAUxYMoU6cvPiv0QXwQqvlO-jjL5oSBIhN8K2maso60mUn74H9vRse8XLgXzo_D4QBwg-ATglA8vywg5oQihk_ABWKUpALy79P4Q4FSzDN4Di5D2MI4GeITMJGEUc7FBVh_OmsSVyfrjXdt6cLOdVXTJXH7jUlm1caExnV7Yjm0qkvmXeWiYhtlk9xYG_boh29a5X-TfLD94M0VOKuVDeb6cKfgazFf58t09f76ls9WqSYE92kFa1aWFCulpNTQCKK15LDimGqDJaupIExnElIpIC4zhJQ2RHGNaloizsgUPIy-O-9-BhP6om2CjqFUZ9wQCsEyTLPoMgWPI6i9C8GbutiNiQsEi32FxX-FEb47uA5la6ojeugs6vcHXQWtbO1Vp5twxIigEkMUsdsR24be-aNMcUahxOQPMyyAlg</recordid><startdate>19930701</startdate><enddate>19930701</enddate><creator>Veronica Morandi</creator><creator>Françoise Fauvel-Lafeve</creator><creator>Chantal Legrand</creator><creator>Legrand, Yves J.</creator><general>Tissue Culture Association, Inc</general><general>Society for In Vitro Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930701</creationdate><title>Role of Thrombospondin in the Adhesion of Human Endothelial Cells in Primary Culture</title><author>Veronica Morandi ; Françoise Fauvel-Lafeve ; Chantal Legrand ; Legrand, Yves J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-d0f5bb42aaa88c0e73cc860d624ce285f4735c98048702b911ace3a6c1f4b1653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Biological and medical sciences</topic><topic>Cell adhesion</topic><topic>Cell Adhesion - physiology</topic><topic>Cell interactions, adhesion</topic><topic>Cell lines</topic><topic>Cells</topic><topic>Cells, Cultured</topic><topic>Cellular Models</topic><topic>Culture Media, Conditioned - pharmacology</topic><topic>Culture Media, Serum-Free - pharmacology</topic><topic>Cultured cells</topic><topic>Endothelial cells</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - physiology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fibronectins - physiology</topic><topic>Fibrosis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gelatins</topic><topic>Humans</topic><topic>Membrane Glycoproteins - physiology</topic><topic>Molecular and cellular biology</topic><topic>Neuroglia</topic><topic>Platelets</topic><topic>Thrombospondins</topic><topic>Umbilical Veins - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Veronica Morandi</creatorcontrib><creatorcontrib>Françoise Fauvel-Lafeve</creatorcontrib><creatorcontrib>Chantal Legrand</creatorcontrib><creatorcontrib>Legrand, Yves J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>In Vitro Cellular & Developmental Biology - Animal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Veronica Morandi</au><au>Françoise Fauvel-Lafeve</au><au>Chantal Legrand</au><au>Legrand, Yves J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Thrombospondin in the Adhesion of Human Endothelial Cells in Primary Culture</atitle><jtitle>In Vitro Cellular & Developmental Biology - Animal</jtitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><date>1993-07-01</date><risdate>1993</risdate><volume>29A</volume><issue>7</issue><spage>585</spage><epage>591</epage><pages>585-591</pages><issn>1071-2690</issn><issn>0883-8364</issn><eissn>1543-706X</eissn><eissn>2327-431X</eissn><coden>ICDBEO</coden><abstract>The role of thrombospondin on the adhesion of endothelial cells in primary culture was studied using a serum-free defined medium or thrombospondin-depleted fetal bovine serum. Under these conditions, only 6% of the cells adhered to gelatin-coated dishes, whereas cells adhering to gelatin in the presence of normal fetal bovine serum were considered as 100% adhesion. The percentage of cells attached to fibronectin or thrombospondin-coated dishes in thrombospondin-depleted serum was 66 and 32%, respectively. The addition of purified platelet thrombospondin to thrombospondin-depleted serum increased the adhesion of endothelial cells to gelatin and to thrombospondin, up to 32 and 59%, respectively, and restored the attachment to fibronectin to the same extent as that observed in the presence of normal serum. In contrast to the attachment, the spreading of the adhering cells was not further influenced by the addition of soluble thrombospondin. Subcultured cells did not require any protein for adhering to gelatin substrata. These observations indicate that thrombospondin plays a major role in the adhesion of endothelial cells in primary culture.</abstract><cop>Largo, MD</cop><pub>Tissue Culture Association, Inc</pub><pmid>8354667</pmid><doi>10.1007/BF02634152</doi><tpages>7</tpages></addata></record> |
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| ispartof | In Vitro Cellular & Developmental Biology - Animal, 1993-07, Vol.29A (7), p.585-591 |
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| language | eng |
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| source | MEDLINE; SpringerNature Journals; JSTOR Archive Collection A-Z Listing |
| subjects | Biological and medical sciences Cell adhesion Cell Adhesion - physiology Cell interactions, adhesion Cell lines Cells Cells, Cultured Cellular Models Culture Media, Conditioned - pharmacology Culture Media, Serum-Free - pharmacology Cultured cells Endothelial cells Endothelium, Vascular - cytology Endothelium, Vascular - physiology Enzyme-Linked Immunosorbent Assay Fibronectins - physiology Fibrosis Fundamental and applied biological sciences. Psychology Gelatins Humans Membrane Glycoproteins - physiology Molecular and cellular biology Neuroglia Platelets Thrombospondins Umbilical Veins - cytology |
| title | Role of Thrombospondin in the Adhesion of Human Endothelial Cells in Primary Culture |
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