A rapid, simple method for calculating equilibrium constants and antibody site concentrations from dilution curves alone

A rapid new practical method for calculating both the antibody-antigen equilibrium constant and the antibody concentration from antibody dilution curve data alone is described. This method is faster than the inhibition curve method for evaluating a humoral immune response. It is particularly suitabl...

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Veröffentlicht in:	Hybridoma 1993-06, Vol.12 (3), p.297-304
Hauptverfasser:	BOUTTEN, B, BAUD, M, VICENTELLI, M. H, DRAY, F, MAMAS, S
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Animals Antibody Affinity Antigen-Antibody Reactions Antigen-antibody reactions, antigen-antibody complexes, antibody-complement and others. Study of affinity. Antigen presentation Binding Sites, Antibody Biological and medical sciences Female Fundamental and applied biological sciences. Psychology Fundamental immunology Immune Sera Kinetics Mice Mice, Inbred BALB C - immunology Molecular immunology Protein Binding SRS-A - immunology
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