A rapid, simple method for calculating equilibrium constants and antibody site concentrations from dilution curves alone
A rapid new practical method for calculating both the antibody-antigen equilibrium constant and the antibody concentration from antibody dilution curve data alone is described. This method is faster than the inhibition curve method for evaluating a humoral immune response. It is particularly suitabl...
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| Veröffentlicht in: | Hybridoma 1993-06, Vol.12 (3), p.297-304 |
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| container_end_page | 304 |
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| container_issue | 3 |
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| container_title | Hybridoma |
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| creator | BOUTTEN, B BAUD, M VICENTELLI, M. H DRAY, F MAMAS, S |
| description | A rapid new practical method for calculating both the antibody-antigen equilibrium constant and the antibody concentration from antibody dilution curve data alone is described. This method is faster than the inhibition curve method for evaluating a humoral immune response. It is particularly suitable for monitoring the immune response of an immunization program. The response is assessed as an immunization index, Abi*Ka. This index is more exact than the antibody titer obtained from dilution curves and independent of the specific activity of the labelled molecule and total activity used in the assay. The method was used to monitor the production of a monoclonal antibody to the sulphide peptide leucotriene including immunization, cloning and purification. |
| doi_str_mv | 10.1089/hyb.1993.12.297 |
| format | Article |
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The method was used to monitor the production of a monoclonal antibody to the sulphide peptide leucotriene including immunization, cloning and purification.</description><identifier>ISSN: 0272-457X</identifier><identifier>EISSN: 2168-7897</identifier><identifier>EISSN: 0272-457X</identifier><identifier>DOI: 10.1089/hyb.1993.12.297</identifier><identifier>PMID: 8359824</identifier><identifier>CODEN: HYBRDY</identifier><language>eng</language><publisher>Larchmont, NY: Liebert</publisher><subject>Algorithms ; Animals ; Antibody Affinity ; Antigen-Antibody Reactions ; Antigen-antibody reactions, antigen-antibody complexes, antibody-complement and others. Study of affinity. Antigen presentation ; Binding Sites, Antibody ; Biological and medical sciences ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Immune Sera ; Kinetics ; Mice ; Mice, Inbred BALB C - immunology ; Molecular immunology ; Protein Binding ; SRS-A - immunology</subject><ispartof>Hybridoma, 1993-06, Vol.12 (3), p.297-304</ispartof><rights>1993 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-82df53fa121d9bbfaf9504dc25be8a201d8534a8b4d18a8da034d356e366c2a73</citedby><cites>FETCH-LOGICAL-c352t-82df53fa121d9bbfaf9504dc25be8a201d8534a8b4d18a8da034d356e366c2a73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3042,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4805446$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8359824$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BOUTTEN, B</creatorcontrib><creatorcontrib>BAUD, M</creatorcontrib><creatorcontrib>VICENTELLI, M. H</creatorcontrib><creatorcontrib>DRAY, F</creatorcontrib><creatorcontrib>MAMAS, S</creatorcontrib><title>A rapid, simple method for calculating equilibrium constants and antibody site concentrations from dilution curves alone</title><title>Hybridoma</title><addtitle>Hybridoma</addtitle><description>A rapid new practical method for calculating both the antibody-antigen equilibrium constant and the antibody concentration from antibody dilution curve data alone is described. This method is faster than the inhibition curve method for evaluating a humoral immune response. It is particularly suitable for monitoring the immune response of an immunization program. The response is assessed as an immunization index, Abi*Ka. This index is more exact than the antibody titer obtained from dilution curves and independent of the specific activity of the labelled molecule and total activity used in the assay. The method was used to monitor the production of a monoclonal antibody to the sulphide peptide leucotriene including immunization, cloning and purification.</description><subject>Algorithms</subject><subject>Animals</subject><subject>Antibody Affinity</subject><subject>Antigen-Antibody Reactions</subject><subject>Antigen-antibody reactions, antigen-antibody complexes, antibody-complement and others. Study of affinity. Antigen presentation</subject><subject>Binding Sites, Antibody</subject><subject>Biological and medical sciences</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Immune Sera</subject><subject>Kinetics</subject><subject>Mice</subject><subject>Mice, Inbred BALB C - immunology</subject><subject>Molecular immunology</subject><subject>Protein Binding</subject><subject>SRS-A - immunology</subject><issn>0272-457X</issn><issn>2168-7897</issn><issn>0272-457X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhYMoYzu6diVkIa6snjyrkuUw-IIBNwruwq08nEiq0pOkZPrfm2aa2bq4XC7nO2dxD0JvKdlTovTV3XHeU635nrI909MztGN0VMOk9PQc7Qib2CDk9OslelXrH0KIZGK6QBeKS62Y2KGHa1zgEN1HXONySB4vvt1lh0Mu2EKyW4IW19_Y328xxbnEbcE2r7XB2iqG1fVpcc7u2AOaP2nWr610V6dwKHnBLqbtdGK7lb--u1Je_Wv0IkCq_s15X6Kfnz_9uPk63H7_8u3m-nawXLI2KOaC5AEoo07Pc4CgJRHOMjl7BYxQpyQXoGbhqALlgHDhuBw9H0fLYOKX6MNj7qHk-83XZpZYrU8JVp-3aiapGe8_-y9IR6k5F7qDV4-gLbnW4oM5lLhAORpKzKkU00sxp1IMZaaX0h3vztHbvHj3xJ9b6Pr7sw61Pz0UWG2sT5hQRAox8n97TpfW</recordid><startdate>19930601</startdate><enddate>19930601</enddate><creator>BOUTTEN, B</creator><creator>BAUD, M</creator><creator>VICENTELLI, M. 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Psychology</topic><topic>Fundamental immunology</topic><topic>Immune Sera</topic><topic>Kinetics</topic><topic>Mice</topic><topic>Mice, Inbred BALB C - immunology</topic><topic>Molecular immunology</topic><topic>Protein Binding</topic><topic>SRS-A - immunology</topic><toplevel>online_resources</toplevel><creatorcontrib>BOUTTEN, B</creatorcontrib><creatorcontrib>BAUD, M</creatorcontrib><creatorcontrib>VICENTELLI, M. 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H</au><au>DRAY, F</au><au>MAMAS, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid, simple method for calculating equilibrium constants and antibody site concentrations from dilution curves alone</atitle><jtitle>Hybridoma</jtitle><addtitle>Hybridoma</addtitle><date>1993-06-01</date><risdate>1993</risdate><volume>12</volume><issue>3</issue><spage>297</spage><epage>304</epage><pages>297-304</pages><issn>0272-457X</issn><eissn>2168-7897</eissn><eissn>0272-457X</eissn><coden>HYBRDY</coden><abstract>A rapid new practical method for calculating both the antibody-antigen equilibrium constant and the antibody concentration from antibody dilution curve data alone is described. This method is faster than the inhibition curve method for evaluating a humoral immune response. It is particularly suitable for monitoring the immune response of an immunization program. The response is assessed as an immunization index, Abi*Ka. 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| fulltext | fulltext |
| identifier | ISSN: 0272-457X |
| ispartof | Hybridoma, 1993-06, Vol.12 (3), p.297-304 |
| issn | 0272-457X 2168-7897 0272-457X |
| language | eng |
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| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Algorithms Animals Antibody Affinity Antigen-Antibody Reactions Antigen-antibody reactions, antigen-antibody complexes, antibody-complement and others. Study of affinity. Antigen presentation Binding Sites, Antibody Biological and medical sciences Female Fundamental and applied biological sciences. Psychology Fundamental immunology Immune Sera Kinetics Mice Mice, Inbred BALB C - immunology Molecular immunology Protein Binding SRS-A - immunology |
| title | A rapid, simple method for calculating equilibrium constants and antibody site concentrations from dilution curves alone |
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