An engineered PGK promoter and lac operator-repressor system for the regulation of gene expression in mammalian cells

Previous reports have demonstrated that the Escherichia coli lac repressor can operate effectively in mammalian cells to repress expression of genes driven by modified viral or metallothionein (MT) promoters. We have developed a more general expression system using the promoter from the PGK1 gene (e...

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Veröffentlicht in:	Gene 1993-08, Vol.130 (2), p.233-239
Hauptverfasser:	Hannan, Garry N., Lehnert, Sigrid A., MacAvoy, Elizabeth S., Jennings, Philip A., Molloy, Peter L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences Biotechnology Cell Nucleus - metabolism Cells, Cultured DNA Escherichia coli expression vector Fundamental and applied biological sciences. Psychology Gene Expression Regulation - drug effects Genetic engineering Genetic technics Genetic Vectors Isopropyl Thiogalactoside - pharmacology Methods. Procedures. Technologies Mice Molecular Sequence Data monkey Mouse phosphoglycerate kinase gene nuclear localisation signal Operator Regions, Genetic Phosphoglycerate Kinase - genetics Plasmids Promoter Regions, Genetic reporter genes Repressor Proteins - genetics Transcription, Genetic Vectors (cloning, transfer, expression). Insertion sequences and transposons
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