Gene cloning and expression of a bone morphogenetic protein derived from a murine osteosarcoma

Based on information from partial amino acid sequences of a protein with bone-inducing activity that was purified from a murine osteosarcoma (Dunn type), a cDNA library of the sarcoma was screened to clone a gene complementary to the protein. The cloned cDNA was amplified and transfected into Chines...

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Veröffentlicht in:	Clinical orthopaedics and related research 1993 (294), p.344-352
Hauptverfasser:	TAKAOKA, K, YOSHIKAWA, H, TWARAGI, Y, INUZUKA, C, KATAYAMA, T, SUGIYAMA, M, TSUJIMOTO, M, NAKANISHI, T, NAKAZATIO, H, HASIMOTO, J, MASUHARA, K, MIYAMOTO, S, SUZUKI, S, ONO, K, MATSUI, M, OIKAWA, S, TSURUOKA, N
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animal tumors. Experimental tumors Animals Base Sequence Biological and medical sciences Bone Morphogenetic Proteins Cloning, Molecular Cricetinae Cricetulus DNA, Circular - genetics Gene Expression Gene Library Growth Substances - genetics Medical sciences Mice Mice, Inbred ICR Molecular Sequence Data Neoplasm Proteins - genetics Neoplasm Proteins - isolation & purification Osteosarcoma - chemistry Osteosarcoma - genetics Polymerase Chain Reaction Proteins - genetics Proteins - isolation & purification Sarcoma, Experimental - chemistry Spontaneous animal tumors Transfection Transforming Growth Factor beta - genetics Tumors
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