Ultra-rapid, simple, sensitive, and economical silica method for extraction of dengue viral RNA from clinical specimens and mosquitoes by reverse transcriptase-polymerase chain reaction
A rapid, simple and efficient single‐tube procedure is described for the isolation of dengue virus RNA from small amount of serum (10 μl) followed by a reverse transcriptase‐polymerase chain reaction (RT‐PCR). Recovery of RNA is based on the lysing and nuclease‐inactivating properties of guanidinium...
Gespeichert in:
| Veröffentlicht in: | Journal of medical virology 1993-06, Vol.40 (2), p.142-145 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 145 |
|---|---|
| container_issue | 2 |
| container_start_page | 142 |
| container_title | Journal of medical virology |
| container_volume | 40 |
| creator | Chungue, Eliane Roche, Claudine Lefevre, Marie-France Barbazan, P. Chanteau, Suzanne |
| description | A rapid, simple and efficient single‐tube procedure is described for the isolation of dengue virus RNA from small amount of serum (10 μl) followed by a reverse transcriptase‐polymerase chain reaction (RT‐PCR). Recovery of RNA is based on the lysing and nuclease‐inactivating properties of guanidinium thiocyanate in the presence of silica. The silica RT‐PCR can be completed within 5 hours starting from RNA extraction to agarose gel electrophoresis. All of the 63 dengue‐3 culture‐positive sera were RT‐PCR positive (virus titres: |
| doi_str_mv | 10.1002/jmv.1890400211 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75918170</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>75918170</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4481-e090ac1fba4eaee62c3e25b057efc1ca7e4320c36112afa6a6ac2e2bb40b9ab43</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhi0EKtvClRuSD4hTs7Xz4STHakUXqtIiRIvExXKcCXWx49ROlu5P498xkNUiTpUPM9Y88449LyGvOFtyxtKTO7dZ8qpmOV44f0IWnNUiqVnJn5IF47lIhODFc3IY4x1jrKrT9IAclKKqRVYsyK9rOwaVBDWY9phG4wYLGKGPZjQbTFXfUtC-985oZZGwGKmD8da3tPOBwgMK6NH4nvqOttB_n4BuTED48-Up7YJ3VFvTz-0DaONQ_a-u8_F-MqOHSJstDbCBEIGiXB91MMOoIiSDt1sHAVOqb5XpEZunvSDPOmUjvNzFI3J99u7L6n1ycbX-sDq9SHSeVzwBVjOledeoHBSASHUGadGwooROc61KyLOU6UxwnqpOCTw6hbRpctbUqsmzI_J21h2Cv58gjtKZqMFa1YOfoiyLmle8ZI-CXIgiY1WF4HIGdfAxBujkEIxTYSs5k39MlWiq_GcqNrzeKU-Ng3aP71zE-ptdXUXccocL1Cbusbwq8Y8ZYvWM_TQWto8Mlecfb_57QjL3mjjCw75XhR9SlFlZyK-Xa_npbP1NFDfncpX9BvDkz3I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16653088</pqid></control><display><type>article</type><title>Ultra-rapid, simple, sensitive, and economical silica method for extraction of dengue viral RNA from clinical specimens and mosquitoes by reverse transcriptase-polymerase chain reaction</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Chungue, Eliane ; Roche, Claudine ; Lefevre, Marie-France ; Barbazan, P. ; Chanteau, Suzanne</creator><creatorcontrib>Chungue, Eliane ; Roche, Claudine ; Lefevre, Marie-France ; Barbazan, P. ; Chanteau, Suzanne</creatorcontrib><description>A rapid, simple and efficient single‐tube procedure is described for the isolation of dengue virus RNA from small amount of serum (10 μl) followed by a reverse transcriptase‐polymerase chain reaction (RT‐PCR). Recovery of RNA is based on the lysing and nuclease‐inactivating properties of guanidinium thiocyanate in the presence of silica. The silica RT‐PCR can be completed within 5 hours starting from RNA extraction to agarose gel electrophoresis. All of the 63 dengue‐3 culture‐positive sera were RT‐PCR positive (virus titres: <102 to 1110.69). Of 33 culture‐ negative acute sera from serologically confirmed dengue fever patients collected during dengue‐3 epidemic, 4 were RT‐PCR‐positive. RT‐PCR was also positive in 29 of 30 dengue‐1 culture‐ positive sera (virus titres range: <102 to 108.69). Dengue‐1 virus was also detected in fieldcaught Aedes aegypti mosquitoes by silica RT‐PCR. © 1993 Wiley‐Liss, Inc.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.1890400211</identifier><identifier>PMID: 7689635</identifier><identifier>CODEN: JMVIDB</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Aedes - microbiology ; Aedes aegypti ; Animals ; Arboviroses ; Biological and medical sciences ; Dengue - microbiology ; dengue diagnosis ; Dengue fevers ; dengue virus ; Dengue Virus - genetics ; Dengue Virus - isolation & purification ; Female ; genomic amplification ; Guanidines ; guanidinium extraction ; Human viral diseases ; Humans ; Infectious diseases ; Medical sciences ; Polymerase Chain Reaction - methods ; RNA isolation ; RNA, Viral - blood ; RNA, Viral - genetics ; RNA, Viral - isolation & purification ; RNA-Directed DNA Polymerase ; Sensitivity and Specificity ; silica RT-PCR ; Silicon Dioxide ; Thiocyanates ; Tropical viral diseases ; Viral diseases</subject><ispartof>Journal of medical virology, 1993-06, Vol.40 (2), p.142-145</ispartof><rights>Copyright © 1993 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4481-e090ac1fba4eaee62c3e25b057efc1ca7e4320c36112afa6a6ac2e2bb40b9ab43</citedby><cites>FETCH-LOGICAL-c4481-e090ac1fba4eaee62c3e25b057efc1ca7e4320c36112afa6a6ac2e2bb40b9ab43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.1890400211$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.1890400211$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4874323$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7689635$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chungue, Eliane</creatorcontrib><creatorcontrib>Roche, Claudine</creatorcontrib><creatorcontrib>Lefevre, Marie-France</creatorcontrib><creatorcontrib>Barbazan, P.</creatorcontrib><creatorcontrib>Chanteau, Suzanne</creatorcontrib><title>Ultra-rapid, simple, sensitive, and economical silica method for extraction of dengue viral RNA from clinical specimens and mosquitoes by reverse transcriptase-polymerase chain reaction</title><title>Journal of medical virology</title><addtitle>J. Med. Virol</addtitle><description>A rapid, simple and efficient single‐tube procedure is described for the isolation of dengue virus RNA from small amount of serum (10 μl) followed by a reverse transcriptase‐polymerase chain reaction (RT‐PCR). Recovery of RNA is based on the lysing and nuclease‐inactivating properties of guanidinium thiocyanate in the presence of silica. The silica RT‐PCR can be completed within 5 hours starting from RNA extraction to agarose gel electrophoresis. All of the 63 dengue‐3 culture‐positive sera were RT‐PCR positive (virus titres: <102 to 1110.69). Of 33 culture‐ negative acute sera from serologically confirmed dengue fever patients collected during dengue‐3 epidemic, 4 were RT‐PCR‐positive. RT‐PCR was also positive in 29 of 30 dengue‐1 culture‐ positive sera (virus titres range: <102 to 108.69). Dengue‐1 virus was also detected in fieldcaught Aedes aegypti mosquitoes by silica RT‐PCR. © 1993 Wiley‐Liss, Inc.</description><subject>Aedes - microbiology</subject><subject>Aedes aegypti</subject><subject>Animals</subject><subject>Arboviroses</subject><subject>Biological and medical sciences</subject><subject>Dengue - microbiology</subject><subject>dengue diagnosis</subject><subject>Dengue fevers</subject><subject>dengue virus</subject><subject>Dengue Virus - genetics</subject><subject>Dengue Virus - isolation & purification</subject><subject>Female</subject><subject>genomic amplification</subject><subject>Guanidines</subject><subject>guanidinium extraction</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Polymerase Chain Reaction - methods</subject><subject>RNA isolation</subject><subject>RNA, Viral - blood</subject><subject>RNA, Viral - genetics</subject><subject>RNA, Viral - isolation & purification</subject><subject>RNA-Directed DNA Polymerase</subject><subject>Sensitivity and Specificity</subject><subject>silica RT-PCR</subject><subject>Silicon Dioxide</subject><subject>Thiocyanates</subject><subject>Tropical viral diseases</subject><subject>Viral diseases</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EKtvClRuSD4hTs7Xz4STHakUXqtIiRIvExXKcCXWx49ROlu5P498xkNUiTpUPM9Y88449LyGvOFtyxtKTO7dZ8qpmOV44f0IWnNUiqVnJn5IF47lIhODFc3IY4x1jrKrT9IAclKKqRVYsyK9rOwaVBDWY9phG4wYLGKGPZjQbTFXfUtC-985oZZGwGKmD8da3tPOBwgMK6NH4nvqOttB_n4BuTED48-Up7YJ3VFvTz-0DaONQ_a-u8_F-MqOHSJstDbCBEIGiXB91MMOoIiSDt1sHAVOqb5XpEZunvSDPOmUjvNzFI3J99u7L6n1ycbX-sDq9SHSeVzwBVjOledeoHBSASHUGadGwooROc61KyLOU6UxwnqpOCTw6hbRpctbUqsmzI_J21h2Cv58gjtKZqMFa1YOfoiyLmle8ZI-CXIgiY1WF4HIGdfAxBujkEIxTYSs5k39MlWiq_GcqNrzeKU-Ng3aP71zE-ptdXUXccocL1Cbusbwq8Y8ZYvWM_TQWto8Mlecfb_57QjL3mjjCw75XhR9SlFlZyK-Xa_npbP1NFDfncpX9BvDkz3I</recordid><startdate>199306</startdate><enddate>199306</enddate><creator>Chungue, Eliane</creator><creator>Roche, Claudine</creator><creator>Lefevre, Marie-France</creator><creator>Barbazan, P.</creator><creator>Chanteau, Suzanne</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199306</creationdate><title>Ultra-rapid, simple, sensitive, and economical silica method for extraction of dengue viral RNA from clinical specimens and mosquitoes by reverse transcriptase-polymerase chain reaction</title><author>Chungue, Eliane ; Roche, Claudine ; Lefevre, Marie-France ; Barbazan, P. ; Chanteau, Suzanne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4481-e090ac1fba4eaee62c3e25b057efc1ca7e4320c36112afa6a6ac2e2bb40b9ab43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Aedes - microbiology</topic><topic>Aedes aegypti</topic><topic>Animals</topic><topic>Arboviroses</topic><topic>Biological and medical sciences</topic><topic>Dengue - microbiology</topic><topic>dengue diagnosis</topic><topic>Dengue fevers</topic><topic>dengue virus</topic><topic>Dengue Virus - genetics</topic><topic>Dengue Virus - isolation & purification</topic><topic>Female</topic><topic>genomic amplification</topic><topic>Guanidines</topic><topic>guanidinium extraction</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Polymerase Chain Reaction - methods</topic><topic>RNA isolation</topic><topic>RNA, Viral - blood</topic><topic>RNA, Viral - genetics</topic><topic>RNA, Viral - isolation & purification</topic><topic>RNA-Directed DNA Polymerase</topic><topic>Sensitivity and Specificity</topic><topic>silica RT-PCR</topic><topic>Silicon Dioxide</topic><topic>Thiocyanates</topic><topic>Tropical viral diseases</topic><topic>Viral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chungue, Eliane</creatorcontrib><creatorcontrib>Roche, Claudine</creatorcontrib><creatorcontrib>Lefevre, Marie-France</creatorcontrib><creatorcontrib>Barbazan, P.</creatorcontrib><creatorcontrib>Chanteau, Suzanne</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chungue, Eliane</au><au>Roche, Claudine</au><au>Lefevre, Marie-France</au><au>Barbazan, P.</au><au>Chanteau, Suzanne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultra-rapid, simple, sensitive, and economical silica method for extraction of dengue viral RNA from clinical specimens and mosquitoes by reverse transcriptase-polymerase chain reaction</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J. Med. Virol</addtitle><date>1993-06</date><risdate>1993</risdate><volume>40</volume><issue>2</issue><spage>142</spage><epage>145</epage><pages>142-145</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><coden>JMVIDB</coden><abstract>A rapid, simple and efficient single‐tube procedure is described for the isolation of dengue virus RNA from small amount of serum (10 μl) followed by a reverse transcriptase‐polymerase chain reaction (RT‐PCR). Recovery of RNA is based on the lysing and nuclease‐inactivating properties of guanidinium thiocyanate in the presence of silica. The silica RT‐PCR can be completed within 5 hours starting from RNA extraction to agarose gel electrophoresis. All of the 63 dengue‐3 culture‐positive sera were RT‐PCR positive (virus titres: <102 to 1110.69). Of 33 culture‐ negative acute sera from serologically confirmed dengue fever patients collected during dengue‐3 epidemic, 4 were RT‐PCR‐positive. RT‐PCR was also positive in 29 of 30 dengue‐1 culture‐ positive sera (virus titres range: <102 to 108.69). Dengue‐1 virus was also detected in fieldcaught Aedes aegypti mosquitoes by silica RT‐PCR. © 1993 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>7689635</pmid><doi>10.1002/jmv.1890400211</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0146-6615 |
| ispartof | Journal of medical virology, 1993-06, Vol.40 (2), p.142-145 |
| issn | 0146-6615 1096-9071 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75918170 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Aedes - microbiology Aedes aegypti Animals Arboviroses Biological and medical sciences Dengue - microbiology dengue diagnosis Dengue fevers dengue virus Dengue Virus - genetics Dengue Virus - isolation & purification Female genomic amplification Guanidines guanidinium extraction Human viral diseases Humans Infectious diseases Medical sciences Polymerase Chain Reaction - methods RNA isolation RNA, Viral - blood RNA, Viral - genetics RNA, Viral - isolation & purification RNA-Directed DNA Polymerase Sensitivity and Specificity silica RT-PCR Silicon Dioxide Thiocyanates Tropical viral diseases Viral diseases |
| title | Ultra-rapid, simple, sensitive, and economical silica method for extraction of dengue viral RNA from clinical specimens and mosquitoes by reverse transcriptase-polymerase chain reaction |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T20%3A58%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ultra-rapid,%20simple,%20sensitive,%20and%20economical%20silica%20method%20for%20extraction%20of%20dengue%20viral%20RNA%20from%20clinical%20specimens%20and%20mosquitoes%20by%20reverse%20transcriptase-polymerase%20chain%20reaction&rft.jtitle=Journal%20of%20medical%20virology&rft.au=Chungue,%20Eliane&rft.date=1993-06&rft.volume=40&rft.issue=2&rft.spage=142&rft.epage=145&rft.pages=142-145&rft.issn=0146-6615&rft.eissn=1096-9071&rft.coden=JMVIDB&rft_id=info:doi/10.1002/jmv.1890400211&rft_dat=%3Cproquest_cross%3E75918170%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16653088&rft_id=info:pmid/7689635&rfr_iscdi=true |