Cryopreservation of Transgenic Mouse Embryos by Ultrarapid Freezing
Two transgenic mice lines were produced by introducing the rat GH antisense transgene and the chicken β-actin promoter/firefly luciferase hybrid gene in our labolatory. We cryopreserved the transgenic embryos, obtained by fertilization in vitro with the sperm of hemizygous males for the transgene, b...
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| Veröffentlicht in: | Experimental Animals 1993/07/01, Vol.42(3), pp.467-470 |
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| container_title | Experimental Animals |
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| creator | ANZAI, Masayuki NAKAGATA, Naomi MATSUMOTO, Kazuya TAKAHASI, Akio TAKAHASI, Yumi MIYATA, Kenji |
| description | Two transgenic mice lines were produced by introducing the rat GH antisense transgene and the chicken β-actin promoter/firefly luciferase hybrid gene in our labolatory. We cryopreserved the transgenic embryos, obtained by fertilization in vitro with the sperm of hemizygous males for the transgene, by the ultrarapid freezing. The survival rates of cryopreserved 2-cell embryos were high (>70%) at thawing in both lines and 53% and 16% of cryopreserved 2-cell embryos, respectively, developed to live young after transferring to oviducts of recipients. Each transgene was detected at about 40% of the live young from two transgenic lines. These results indicated that the cryopreservation of embryos by ultrarapid freezing was valuable for sustaining transgenic mouse lines without genetic contamination. |
| doi_str_mv | 10.1538/expanim1978.42.3_467 |
| format | Article |
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We cryopreserved the transgenic embryos, obtained by fertilization in vitro with the sperm of hemizygous males for the transgene, by the ultrarapid freezing. The survival rates of cryopreserved 2-cell embryos were high (>70%) at thawing in both lines and 53% and 16% of cryopreserved 2-cell embryos, respectively, developed to live young after transferring to oviducts of recipients. Each transgene was detected at about 40% of the live young from two transgenic lines. 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These results indicated that the cryopreservation of embryos by ultrarapid freezing was valuable for sustaining transgenic mouse lines without genetic contamination.</description><subject>Animals</subject><subject>Cryopreservation - methods</subject><subject>Embryo, Mammalian</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>mouse embryos</subject><subject>transgenic mouse</subject><subject>ultrarapid freezing</subject><issn>0007-5124</issn><issn>1341-1357</issn><issn>1881-7122</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9LwzAYh4MoOtRvoNCTt868TdIk3mQ4FRQveg5p-nZG-s-kE-ent7oxRARPIeT3PISHkBOgUxBMneN7b1vfgJZqyrMpMzyXO2QCSkEqIct2yYRSKlMBGT8gxzG-jFeqgYIU-2RfMcGZhAmZzcKq6wNGDG928F2bdFXyGGwbF9h6l9x3y4jJVVOMs5gUq-SpHoINtvdlMg-IH75dHJG9ytYRjzfnIXmaXz3ObtK7h-vb2eVd6jhkQ4qobakyYZkuhaKcKQkgXE6zomAq18wxVnCouLUyz4USSpeFplhpKKrcAjskZ2tvH7rXJcbBND46rGvb4vhNI4UGRjUdh3w9dKGLMWBl-uAbG1YGqPnKZ37kMzwz3_lG7HTjXxYNlltoE2t8v_ildX74jjYm8fV_8us1_BIHu8Ct3IbBuxr_gDbkduGebTDYsk85R5p0</recordid><startdate>19930701</startdate><enddate>19930701</enddate><creator>ANZAI, Masayuki</creator><creator>NAKAGATA, Naomi</creator><creator>MATSUMOTO, Kazuya</creator><creator>TAKAHASI, Akio</creator><creator>TAKAHASI, Yumi</creator><creator>MIYATA, Kenji</creator><general>Japanese Association for Laboratory Animal Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930701</creationdate><title>Cryopreservation of Transgenic Mouse Embryos by Ultrarapid Freezing</title><author>ANZAI, Masayuki ; NAKAGATA, Naomi ; MATSUMOTO, Kazuya ; TAKAHASI, Akio ; TAKAHASI, Yumi ; MIYATA, Kenji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-ee9ad825a39d5804387115c602bb38693c33b41f4aa76658589db90ef91bf6a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng ; jpn</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Cryopreservation - methods</topic><topic>Embryo, Mammalian</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>mouse embryos</topic><topic>transgenic mouse</topic><topic>ultrarapid freezing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ANZAI, Masayuki</creatorcontrib><creatorcontrib>NAKAGATA, Naomi</creatorcontrib><creatorcontrib>MATSUMOTO, Kazuya</creatorcontrib><creatorcontrib>TAKAHASI, Akio</creatorcontrib><creatorcontrib>TAKAHASI, Yumi</creatorcontrib><creatorcontrib>MIYATA, Kenji</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental Animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ANZAI, Masayuki</au><au>NAKAGATA, Naomi</au><au>MATSUMOTO, Kazuya</au><au>TAKAHASI, Akio</au><au>TAKAHASI, Yumi</au><au>MIYATA, Kenji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryopreservation of Transgenic Mouse Embryos by Ultrarapid Freezing</atitle><jtitle>Experimental Animals</jtitle><addtitle>Experimental Animals</addtitle><date>1993-07-01</date><risdate>1993</risdate><volume>42</volume><issue>3</issue><spage>467</spage><epage>470</epage><pages>467-470</pages><artnum>467</artnum><issn>0007-5124</issn><issn>1341-1357</issn><eissn>1881-7122</eissn><abstract>Two transgenic mice lines were produced by introducing the rat GH antisense transgene and the chicken β-actin promoter/firefly luciferase hybrid gene in our labolatory. 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| identifier | ISSN: 0007-5124 |
| ispartof | Experimental Animals, 1993/07/01, Vol.42(3), pp.467-470 |
| issn | 0007-5124 1341-1357 1881-7122 |
| language | eng ; jpn |
| recordid | cdi_proquest_miscellaneous_75913090 |
| source | J-STAGE Free; MEDLINE |
| subjects | Animals Cryopreservation - methods Embryo, Mammalian Female Fertilization in Vitro Male Mice Mice, Transgenic mouse embryos transgenic mouse ultrarapid freezing |
| title | Cryopreservation of Transgenic Mouse Embryos by Ultrarapid Freezing |
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