Noninvasive up-regulation of angiopoietin-2 and fibroblast growth factor-2 in bone marrow by pulsed electromagnetic field therapy
Pulsed electromagnetic field (PEMF) therapy has been widely used in clinical practice for bone fracture healing. However, the mechanism of its action remains to be elucidated. Our object was to investigate the mechanism by which PEMF accelerates bone fracture healing. We used 20 mice in this study....
Gespeichert in:
| Veröffentlicht in: | Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association 2010-09, Vol.15 (5), p.661-665 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 665 |
|---|---|
| container_issue | 5 |
| container_start_page | 661 |
| container_title | Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association |
| container_volume | 15 |
| creator | Goto, Tsuyoshi Fujioka, Mikihiro Ishida, Masashi Kuribayashi, Masaaki Ueshima, Keiichiro Kubo, Toshikazu |
| description | Pulsed electromagnetic field (PEMF) therapy has been widely used in clinical practice for bone fracture healing. However, the mechanism of its action remains to be elucidated. Our object was to investigate the mechanism by which PEMF accelerates bone fracture healing.
We used 20 mice in this study. Ten mice received PEMF for 10 h/day for 1 week via the coils of a PEMF stimulation device (PEMF group), while the remaining 10 mice did not (control group). The femurs were harvested immediately after euthanasia to examine the proteins included in the bone marrow. The proteins examined by Western blotting were growth factors with angiogenetic activities, including tunica interna endothelial cell kinase-2, angiopoietin-1, angiopoietin-2, fibroblast growth factor-2, and vascular endothelial growth factor. The expression levels of angiogenesis-related proteins extracted from the bone marrow of each mouse were compared.
The expression levels of angiopoietin-2 and fibroblast growth factor-2 were significantly higher in the PEMF group than in the control group. This difference suggests that PEMF may induce an angiogenesis-prone environment in the bone marrow. Such angiogenesis acceleration represents one possible mechanism for the acceleration of bone fracture healing by PEMF. There were no significant differences between the two groups for the expression levels of tunica interna endothelial cell kinase-2, angiopoietin-1, and vascular endothelial growth factor. The lack of increase in tunica interna endothelial cell kinase-2 expression may indicate that PEMF does not unnecessarily increase blood vessels in normal bone marrow. The lack of an increase in the expression level of vascular endothelial growth factor suggests that PEMF does not have invasive effects including the induction of hypoxic conditions and inflammation on the bone marrow.
The angiogenesis-promoting function of PEMF may contribute to its mechanism to noninvasively accelerate bone fracture healing. |
| doi_str_mv | 10.1007/s00776-010-1510-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_759130089</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0949265815309040</els_id><sourcerecordid>759130089</sourcerecordid><originalsourceid>FETCH-LOGICAL-c541t-141628deb0f94ac19e2ff290f8a591f8f859b70b573b16d3ac6b24cf1ec497383</originalsourceid><addsrcrecordid>eNp9UU1v1DAQjRCILoUfwAVZXDgFxo7zYXFCFdBKFVzgbNnOeOsqawc7WbRH_jlTpYDEoZexRu_D9ntV9ZLDWw7Qvys0-q4GDjVvacCjasdl09UCRPO42oGSqhZdO5xVz0q5BeB9q9qn1ZkA1TZKDLvq15cUQzyaEo7I1rnOuF8ns4QUWfLMxH1Icwq4hFgLWkfmg83JTqYsbJ_Tz-WGeeOWlAkOkdkUkR1MJoTZE5vXqeDIcEK35HQw-0hOjjxwGtlyg9nMp-fVE2-I9uL-PK--f_r47eKyvv76-eriw3XtWsmXmkveiWFEC15J47hC4b1Q4AfTKu4HP7TK9mDbvrG8GxvjOiuk8xydVH0zNOfVm813zunHimXRh1AcTpOJmNaiKRreAAyKmK__Y96mNUd6nB5AckpOSiLxjeRyKiWj13MO9POT5qDv2tFbOxrudmpHA2le3Ruv9oDjX8WfOoggNkIhKO4x_7v5Idf3mwgpvWMgUXEBo8MxZMpdjyk8oP4Nmeeu7w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>804153944</pqid></control><display><type>article</type><title>Noninvasive up-regulation of angiopoietin-2 and fibroblast growth factor-2 in bone marrow by pulsed electromagnetic field therapy</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><source>Alma/SFX Local Collection</source><creator>Goto, Tsuyoshi ; Fujioka, Mikihiro ; Ishida, Masashi ; Kuribayashi, Masaaki ; Ueshima, Keiichiro ; Kubo, Toshikazu</creator><creatorcontrib>Goto, Tsuyoshi ; Fujioka, Mikihiro ; Ishida, Masashi ; Kuribayashi, Masaaki ; Ueshima, Keiichiro ; Kubo, Toshikazu</creatorcontrib><description>Pulsed electromagnetic field (PEMF) therapy has been widely used in clinical practice for bone fracture healing. However, the mechanism of its action remains to be elucidated. Our object was to investigate the mechanism by which PEMF accelerates bone fracture healing.
We used 20 mice in this study. Ten mice received PEMF for 10 h/day for 1 week via the coils of a PEMF stimulation device (PEMF group), while the remaining 10 mice did not (control group). The femurs were harvested immediately after euthanasia to examine the proteins included in the bone marrow. The proteins examined by Western blotting were growth factors with angiogenetic activities, including tunica interna endothelial cell kinase-2, angiopoietin-1, angiopoietin-2, fibroblast growth factor-2, and vascular endothelial growth factor. The expression levels of angiogenesis-related proteins extracted from the bone marrow of each mouse were compared.
The expression levels of angiopoietin-2 and fibroblast growth factor-2 were significantly higher in the PEMF group than in the control group. This difference suggests that PEMF may induce an angiogenesis-prone environment in the bone marrow. Such angiogenesis acceleration represents one possible mechanism for the acceleration of bone fracture healing by PEMF. There were no significant differences between the two groups for the expression levels of tunica interna endothelial cell kinase-2, angiopoietin-1, and vascular endothelial growth factor. The lack of increase in tunica interna endothelial cell kinase-2 expression may indicate that PEMF does not unnecessarily increase blood vessels in normal bone marrow. The lack of an increase in the expression level of vascular endothelial growth factor suggests that PEMF does not have invasive effects including the induction of hypoxic conditions and inflammation on the bone marrow.
The angiogenesis-promoting function of PEMF may contribute to its mechanism to noninvasively accelerate bone fracture healing.</description><identifier>ISSN: 0949-2658</identifier><identifier>EISSN: 1436-2023</identifier><identifier>DOI: 10.1007/s00776-010-1510-0</identifier><identifier>PMID: 20953928</identifier><language>eng</language><publisher>Japan: Elsevier B.V</publisher><subject>Angiopoietin-2 - metabolism ; Animals ; Bone Marrow - metabolism ; Femur ; Fibroblast Growth Factor 2 - metabolism ; Fracture Healing ; Magnetic Field Therapy ; Male ; Medicine ; Medicine & Public Health ; Mice ; Mice, Inbred ICR ; Neovascularization, Physiologic ; Original Article ; Orthopedics ; Receptor, TIE-2 - metabolism ; Rheumatology ; Up-Regulation ; Vascular Endothelial Growth Factor A - metabolism</subject><ispartof>Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association, 2010-09, Vol.15 (5), p.661-665</ispartof><rights>2010 The Japanese Orthopaedic Association</rights><rights>The Japanese Orthopaedic Association 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c541t-141628deb0f94ac19e2ff290f8a591f8f859b70b573b16d3ac6b24cf1ec497383</citedby><cites>FETCH-LOGICAL-c541t-141628deb0f94ac19e2ff290f8a591f8f859b70b573b16d3ac6b24cf1ec497383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00776-010-1510-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00776-010-1510-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20953928$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goto, Tsuyoshi</creatorcontrib><creatorcontrib>Fujioka, Mikihiro</creatorcontrib><creatorcontrib>Ishida, Masashi</creatorcontrib><creatorcontrib>Kuribayashi, Masaaki</creatorcontrib><creatorcontrib>Ueshima, Keiichiro</creatorcontrib><creatorcontrib>Kubo, Toshikazu</creatorcontrib><title>Noninvasive up-regulation of angiopoietin-2 and fibroblast growth factor-2 in bone marrow by pulsed electromagnetic field therapy</title><title>Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association</title><addtitle>J Orthop Sci</addtitle><addtitle>J Orthop Sci</addtitle><description>Pulsed electromagnetic field (PEMF) therapy has been widely used in clinical practice for bone fracture healing. However, the mechanism of its action remains to be elucidated. Our object was to investigate the mechanism by which PEMF accelerates bone fracture healing.
We used 20 mice in this study. Ten mice received PEMF for 10 h/day for 1 week via the coils of a PEMF stimulation device (PEMF group), while the remaining 10 mice did not (control group). The femurs were harvested immediately after euthanasia to examine the proteins included in the bone marrow. The proteins examined by Western blotting were growth factors with angiogenetic activities, including tunica interna endothelial cell kinase-2, angiopoietin-1, angiopoietin-2, fibroblast growth factor-2, and vascular endothelial growth factor. The expression levels of angiogenesis-related proteins extracted from the bone marrow of each mouse were compared.
The expression levels of angiopoietin-2 and fibroblast growth factor-2 were significantly higher in the PEMF group than in the control group. This difference suggests that PEMF may induce an angiogenesis-prone environment in the bone marrow. Such angiogenesis acceleration represents one possible mechanism for the acceleration of bone fracture healing by PEMF. There were no significant differences between the two groups for the expression levels of tunica interna endothelial cell kinase-2, angiopoietin-1, and vascular endothelial growth factor. The lack of increase in tunica interna endothelial cell kinase-2 expression may indicate that PEMF does not unnecessarily increase blood vessels in normal bone marrow. The lack of an increase in the expression level of vascular endothelial growth factor suggests that PEMF does not have invasive effects including the induction of hypoxic conditions and inflammation on the bone marrow.
The angiogenesis-promoting function of PEMF may contribute to its mechanism to noninvasively accelerate bone fracture healing.</description><subject>Angiopoietin-2 - metabolism</subject><subject>Animals</subject><subject>Bone Marrow - metabolism</subject><subject>Femur</subject><subject>Fibroblast Growth Factor 2 - metabolism</subject><subject>Fracture Healing</subject><subject>Magnetic Field Therapy</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Neovascularization, Physiologic</subject><subject>Original Article</subject><subject>Orthopedics</subject><subject>Receptor, TIE-2 - metabolism</subject><subject>Rheumatology</subject><subject>Up-Regulation</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><issn>0949-2658</issn><issn>1436-2023</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp9UU1v1DAQjRCILoUfwAVZXDgFxo7zYXFCFdBKFVzgbNnOeOsqawc7WbRH_jlTpYDEoZexRu_D9ntV9ZLDWw7Qvys0-q4GDjVvacCjasdl09UCRPO42oGSqhZdO5xVz0q5BeB9q9qn1ZkA1TZKDLvq15cUQzyaEo7I1rnOuF8ns4QUWfLMxH1Icwq4hFgLWkfmg83JTqYsbJ_Tz-WGeeOWlAkOkdkUkR1MJoTZE5vXqeDIcEK35HQw-0hOjjxwGtlyg9nMp-fVE2-I9uL-PK--f_r47eKyvv76-eriw3XtWsmXmkveiWFEC15J47hC4b1Q4AfTKu4HP7TK9mDbvrG8GxvjOiuk8xydVH0zNOfVm813zunHimXRh1AcTpOJmNaiKRreAAyKmK__Y96mNUd6nB5AckpOSiLxjeRyKiWj13MO9POT5qDv2tFbOxrudmpHA2le3Ruv9oDjX8WfOoggNkIhKO4x_7v5Idf3mwgpvWMgUXEBo8MxZMpdjyk8oP4Nmeeu7w</recordid><startdate>20100901</startdate><enddate>20100901</enddate><creator>Goto, Tsuyoshi</creator><creator>Fujioka, Mikihiro</creator><creator>Ishida, Masashi</creator><creator>Kuribayashi, Masaaki</creator><creator>Ueshima, Keiichiro</creator><creator>Kubo, Toshikazu</creator><general>Elsevier B.V</general><general>Springer Japan</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20100901</creationdate><title>Noninvasive up-regulation of angiopoietin-2 and fibroblast growth factor-2 in bone marrow by pulsed electromagnetic field therapy</title><author>Goto, Tsuyoshi ; Fujioka, Mikihiro ; Ishida, Masashi ; Kuribayashi, Masaaki ; Ueshima, Keiichiro ; Kubo, Toshikazu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c541t-141628deb0f94ac19e2ff290f8a591f8f859b70b573b16d3ac6b24cf1ec497383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Angiopoietin-2 - metabolism</topic><topic>Animals</topic><topic>Bone Marrow - metabolism</topic><topic>Femur</topic><topic>Fibroblast Growth Factor 2 - metabolism</topic><topic>Fracture Healing</topic><topic>Magnetic Field Therapy</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Neovascularization, Physiologic</topic><topic>Original Article</topic><topic>Orthopedics</topic><topic>Receptor, TIE-2 - metabolism</topic><topic>Rheumatology</topic><topic>Up-Regulation</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goto, Tsuyoshi</creatorcontrib><creatorcontrib>Fujioka, Mikihiro</creatorcontrib><creatorcontrib>Ishida, Masashi</creatorcontrib><creatorcontrib>Kuribayashi, Masaaki</creatorcontrib><creatorcontrib>Ueshima, Keiichiro</creatorcontrib><creatorcontrib>Kubo, Toshikazu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goto, Tsuyoshi</au><au>Fujioka, Mikihiro</au><au>Ishida, Masashi</au><au>Kuribayashi, Masaaki</au><au>Ueshima, Keiichiro</au><au>Kubo, Toshikazu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Noninvasive up-regulation of angiopoietin-2 and fibroblast growth factor-2 in bone marrow by pulsed electromagnetic field therapy</atitle><jtitle>Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association</jtitle><stitle>J Orthop Sci</stitle><addtitle>J Orthop Sci</addtitle><date>2010-09-01</date><risdate>2010</risdate><volume>15</volume><issue>5</issue><spage>661</spage><epage>665</epage><pages>661-665</pages><issn>0949-2658</issn><eissn>1436-2023</eissn><abstract>Pulsed electromagnetic field (PEMF) therapy has been widely used in clinical practice for bone fracture healing. However, the mechanism of its action remains to be elucidated. Our object was to investigate the mechanism by which PEMF accelerates bone fracture healing.
We used 20 mice in this study. Ten mice received PEMF for 10 h/day for 1 week via the coils of a PEMF stimulation device (PEMF group), while the remaining 10 mice did not (control group). The femurs were harvested immediately after euthanasia to examine the proteins included in the bone marrow. The proteins examined by Western blotting were growth factors with angiogenetic activities, including tunica interna endothelial cell kinase-2, angiopoietin-1, angiopoietin-2, fibroblast growth factor-2, and vascular endothelial growth factor. The expression levels of angiogenesis-related proteins extracted from the bone marrow of each mouse were compared.
The expression levels of angiopoietin-2 and fibroblast growth factor-2 were significantly higher in the PEMF group than in the control group. This difference suggests that PEMF may induce an angiogenesis-prone environment in the bone marrow. Such angiogenesis acceleration represents one possible mechanism for the acceleration of bone fracture healing by PEMF. There were no significant differences between the two groups for the expression levels of tunica interna endothelial cell kinase-2, angiopoietin-1, and vascular endothelial growth factor. The lack of increase in tunica interna endothelial cell kinase-2 expression may indicate that PEMF does not unnecessarily increase blood vessels in normal bone marrow. The lack of an increase in the expression level of vascular endothelial growth factor suggests that PEMF does not have invasive effects including the induction of hypoxic conditions and inflammation on the bone marrow.
The angiogenesis-promoting function of PEMF may contribute to its mechanism to noninvasively accelerate bone fracture healing.</abstract><cop>Japan</cop><pub>Elsevier B.V</pub><pmid>20953928</pmid><doi>10.1007/s00776-010-1510-0</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0949-2658 |
| ispartof | Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association, 2010-09, Vol.15 (5), p.661-665 |
| issn | 0949-2658 1436-2023 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_759130089 |
| source | MEDLINE; Springer Nature - Complete Springer Journals; Alma/SFX Local Collection |
| subjects | Angiopoietin-2 - metabolism Animals Bone Marrow - metabolism Femur Fibroblast Growth Factor 2 - metabolism Fracture Healing Magnetic Field Therapy Male Medicine Medicine & Public Health Mice Mice, Inbred ICR Neovascularization, Physiologic Original Article Orthopedics Receptor, TIE-2 - metabolism Rheumatology Up-Regulation Vascular Endothelial Growth Factor A - metabolism |
| title | Noninvasive up-regulation of angiopoietin-2 and fibroblast growth factor-2 in bone marrow by pulsed electromagnetic field therapy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T04%3A42%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Noninvasive%20up-regulation%20of%20angiopoietin-2%20and%20fibroblast%20growth%20factor-2%20in%20bone%20marrow%20by%20pulsed%20electromagnetic%20field%20therapy&rft.jtitle=Journal%20of%20orthopaedic%20science%20:%20official%20journal%20of%20the%20Japanese%20Orthopaedic%20Association&rft.au=Goto,%20Tsuyoshi&rft.date=2010-09-01&rft.volume=15&rft.issue=5&rft.spage=661&rft.epage=665&rft.pages=661-665&rft.issn=0949-2658&rft.eissn=1436-2023&rft_id=info:doi/10.1007/s00776-010-1510-0&rft_dat=%3Cproquest_cross%3E759130089%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=804153944&rft_id=info:pmid/20953928&rft_els_id=S0949265815309040&rfr_iscdi=true |