A comparative study of the reversed-phase HPLC retention behaviour of S-adenosyl- l-methionine and its related metabolites on Hypersil ODS and Supelcosil™ LC-ABZ stationary phases
S-Adenosyl- l-methionine (SAM) and its metabolites S-adenosyl- l-homocysteine (SAH) and methylthioadenosine (MTA) are endogenous compounds that are heavily involved in a variety of biochemical processes, and have therefore been the target for several assays in body fluids and tissues. Reversed-phase...
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Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 1993-04, Vol.11 (4), p.361-366 |
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creator | Valkó, Klára Hamedani, Morteza P. Ascah, Tracy L. Gibbons, William A. |
description | S-Adenosyl-
l-methionine (SAM) and its metabolites S-adenosyl-
l-homocysteine (SAH) and methylthioadenosine (MTA) are endogenous compounds that are heavily involved in a variety of biochemical processes, and have therefore been the target for several assays in body fluids and tissues. Reversed-phase chromatographic behaviour of SAM and its metabolites has been studied by using Supelcosil™ LC-ABZ column, specially designed for analysis of acidic, basic, zwitterionic and neutral compounds, and on a Hypersil ODS column as a function of mobile phase pH. The retentions of the compounds, expressed by the capacity ratio (
k′), are measured on both column with mobile phases comprised of 10% acetonitrile and 10 mM ammonium formate buffer with pH values ranging from 2 to 9. Higher selectivity is observed on Supelcosil LC-ABZ within pH range 4–6. Different retention properties are observed at very low pH and seemed as if the Supelcosil LC-ABZ column reduced the effect of the mobile phase pH by about 1 pH unit. Whilst the Supelcosil column can be recommended for the routine analysis of SAM and its related metabolises in biological fluids by using mobile phase pH 5, the Hypersil ODS column may be suggested for use with mobile phase pH values of 3–4. |
doi_str_mv | 10.1016/0731-7085(93)80029-Z |
format | Article |
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l-methionine (SAM) and its metabolites S-adenosyl-
l-homocysteine (SAH) and methylthioadenosine (MTA) are endogenous compounds that are heavily involved in a variety of biochemical processes, and have therefore been the target for several assays in body fluids and tissues. Reversed-phase chromatographic behaviour of SAM and its metabolites has been studied by using Supelcosil™ LC-ABZ column, specially designed for analysis of acidic, basic, zwitterionic and neutral compounds, and on a Hypersil ODS column as a function of mobile phase pH. The retentions of the compounds, expressed by the capacity ratio (
k′), are measured on both column with mobile phases comprised of 10% acetonitrile and 10 mM ammonium formate buffer with pH values ranging from 2 to 9. Higher selectivity is observed on Supelcosil LC-ABZ within pH range 4–6. Different retention properties are observed at very low pH and seemed as if the Supelcosil LC-ABZ column reduced the effect of the mobile phase pH by about 1 pH unit. Whilst the Supelcosil column can be recommended for the routine analysis of SAM and its related metabolises in biological fluids by using mobile phase pH 5, the Hypersil ODS column may be suggested for use with mobile phase pH values of 3–4.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/0731-7085(93)80029-Z</identifier><identifier>PMID: 8395220</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Adenosine - analysis ; Chromatography, High Pressure Liquid ; Deoxyadenosines - analysis ; Deoxyuridine - analogs & derivatives ; Deoxyuridine - analysis ; dependence of the RP-HPLC retention on pH ; Hydrogen-Ion Concentration ; reversed-phase HPLC ; S-Adenosyl- l-methionine and related metabolites ; S-Adenosylhomocysteine - analysis ; S-Adenosylmethionine - analysis ; S-Adenosylmethionine - chemistry ; Silicon Dioxide ; Spectrophotometry, Ultraviolet ; Thionucleosides - analysis</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 1993-04, Vol.11 (4), p.361-366</ispartof><rights>1993</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-a561a27538bd51d9dd6395086d8b03e2796599fc9f830c319144116d158f4b143</citedby><cites>FETCH-LOGICAL-c357t-a561a27538bd51d9dd6395086d8b03e2796599fc9f830c319144116d158f4b143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0731-7085(93)80029-Z$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8395220$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valkó, Klára</creatorcontrib><creatorcontrib>Hamedani, Morteza P.</creatorcontrib><creatorcontrib>Ascah, Tracy L.</creatorcontrib><creatorcontrib>Gibbons, William A.</creatorcontrib><title>A comparative study of the reversed-phase HPLC retention behaviour of S-adenosyl- l-methionine and its related metabolites on Hypersil ODS and Supelcosil™ LC-ABZ stationary phases</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>S-Adenosyl-
l-methionine (SAM) and its metabolites S-adenosyl-
l-homocysteine (SAH) and methylthioadenosine (MTA) are endogenous compounds that are heavily involved in a variety of biochemical processes, and have therefore been the target for several assays in body fluids and tissues. Reversed-phase chromatographic behaviour of SAM and its metabolites has been studied by using Supelcosil™ LC-ABZ column, specially designed for analysis of acidic, basic, zwitterionic and neutral compounds, and on a Hypersil ODS column as a function of mobile phase pH. The retentions of the compounds, expressed by the capacity ratio (
k′), are measured on both column with mobile phases comprised of 10% acetonitrile and 10 mM ammonium formate buffer with pH values ranging from 2 to 9. Higher selectivity is observed on Supelcosil LC-ABZ within pH range 4–6. Different retention properties are observed at very low pH and seemed as if the Supelcosil LC-ABZ column reduced the effect of the mobile phase pH by about 1 pH unit. Whilst the Supelcosil column can be recommended for the routine analysis of SAM and its related metabolises in biological fluids by using mobile phase pH 5, the Hypersil ODS column may be suggested for use with mobile phase pH values of 3–4.</description><subject>Adenosine - analysis</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Deoxyadenosines - analysis</subject><subject>Deoxyuridine - analogs & derivatives</subject><subject>Deoxyuridine - analysis</subject><subject>dependence of the RP-HPLC retention on pH</subject><subject>Hydrogen-Ion Concentration</subject><subject>reversed-phase HPLC</subject><subject>S-Adenosyl- l-methionine and related metabolites</subject><subject>S-Adenosylhomocysteine - analysis</subject><subject>S-Adenosylmethionine - analysis</subject><subject>S-Adenosylmethionine - chemistry</subject><subject>Silicon Dioxide</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Thionucleosides - analysis</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uc2q1DAUDqJc5159A4WsRBfRpGnaZiOM488IA1cYBZlNSJtTJtI2vUk6MHuf5L6DL-STmM4Md-nqwDnf3-FD6AWjbxllxTtackZKWonXkr-pKM0k2T1CC1aVnGRF_vMxWjxAnqLrEH5RSgWT-RW6qrgUWUYX6M8SN64ftdfRHgCHOJkjdi2Oe8AeDuADGDLudQC8_rZZpV2EIVo34Br2-mDd5Gf4lmgDgwvHjuCO9BD3CWIHwHow2MaQeJ2OYHA66dp1NkLASWR9HJOF7fDtx-0Ju51G6BqXVn9_3-PNiiw_7FIqPVtqf8SnKOEZetLqLsDzy7xBPz5_-r5ak83tl6-r5YY0XJSRaFEwnZWCV7URzEhjivQ3rQpT1ZRDVspCSNk2sq04bTiTLM8ZKwwTVZvXLOc36NVZd_TuboIQVW9DA12nB3BTUKWQVFZFloD5Gdh4F4KHVo3e9imwYlTNbam5CjVXoSRXp7bULtFeXvSnugfzQLrUk-7vz3dITx4seBUaC0MDxnpoojLO_t_gH8iBptc</recordid><startdate>19930401</startdate><enddate>19930401</enddate><creator>Valkó, Klára</creator><creator>Hamedani, Morteza P.</creator><creator>Ascah, Tracy L.</creator><creator>Gibbons, William A.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930401</creationdate><title>A comparative study of the reversed-phase HPLC retention behaviour of S-adenosyl- l-methionine and its related metabolites on Hypersil ODS and Supelcosil™ LC-ABZ stationary phases</title><author>Valkó, Klára ; Hamedani, Morteza P. ; Ascah, Tracy L. ; Gibbons, William A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-a561a27538bd51d9dd6395086d8b03e2796599fc9f830c319144116d158f4b143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adenosine - analysis</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Deoxyadenosines - analysis</topic><topic>Deoxyuridine - analogs & derivatives</topic><topic>Deoxyuridine - analysis</topic><topic>dependence of the RP-HPLC retention on pH</topic><topic>Hydrogen-Ion Concentration</topic><topic>reversed-phase HPLC</topic><topic>S-Adenosyl- l-methionine and related metabolites</topic><topic>S-Adenosylhomocysteine - analysis</topic><topic>S-Adenosylmethionine - analysis</topic><topic>S-Adenosylmethionine - chemistry</topic><topic>Silicon Dioxide</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Thionucleosides - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valkó, Klára</creatorcontrib><creatorcontrib>Hamedani, Morteza P.</creatorcontrib><creatorcontrib>Ascah, Tracy L.</creatorcontrib><creatorcontrib>Gibbons, William A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valkó, Klára</au><au>Hamedani, Morteza P.</au><au>Ascah, Tracy L.</au><au>Gibbons, William A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparative study of the reversed-phase HPLC retention behaviour of S-adenosyl- l-methionine and its related metabolites on Hypersil ODS and Supelcosil™ LC-ABZ stationary phases</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>1993-04-01</date><risdate>1993</risdate><volume>11</volume><issue>4</issue><spage>361</spage><epage>366</epage><pages>361-366</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><abstract>S-Adenosyl-
l-methionine (SAM) and its metabolites S-adenosyl-
l-homocysteine (SAH) and methylthioadenosine (MTA) are endogenous compounds that are heavily involved in a variety of biochemical processes, and have therefore been the target for several assays in body fluids and tissues. Reversed-phase chromatographic behaviour of SAM and its metabolites has been studied by using Supelcosil™ LC-ABZ column, specially designed for analysis of acidic, basic, zwitterionic and neutral compounds, and on a Hypersil ODS column as a function of mobile phase pH. The retentions of the compounds, expressed by the capacity ratio (
k′), are measured on both column with mobile phases comprised of 10% acetonitrile and 10 mM ammonium formate buffer with pH values ranging from 2 to 9. Higher selectivity is observed on Supelcosil LC-ABZ within pH range 4–6. Different retention properties are observed at very low pH and seemed as if the Supelcosil LC-ABZ column reduced the effect of the mobile phase pH by about 1 pH unit. Whilst the Supelcosil column can be recommended for the routine analysis of SAM and its related metabolises in biological fluids by using mobile phase pH 5, the Hypersil ODS column may be suggested for use with mobile phase pH values of 3–4.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>8395220</pmid><doi>10.1016/0731-7085(93)80029-Z</doi><tpages>6</tpages></addata></record> |
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subjects | Adenosine - analysis Chromatography, High Pressure Liquid Deoxyadenosines - analysis Deoxyuridine - analogs & derivatives Deoxyuridine - analysis dependence of the RP-HPLC retention on pH Hydrogen-Ion Concentration reversed-phase HPLC S-Adenosyl- l-methionine and related metabolites S-Adenosylhomocysteine - analysis S-Adenosylmethionine - analysis S-Adenosylmethionine - chemistry Silicon Dioxide Spectrophotometry, Ultraviolet Thionucleosides - analysis |
title | A comparative study of the reversed-phase HPLC retention behaviour of S-adenosyl- l-methionine and its related metabolites on Hypersil ODS and Supelcosil™ LC-ABZ stationary phases |
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