Purification of the structural and nonstructural proteins of St. Louis encephalitis virus
We have developed a procedure for purifying both the structural and nonstructural proteins of flaviviruses from lysates of infected cell cultures. The procedure involves: (l)immunoprecipitation to concentrate viral proteins and eliminate most of the cellular proteins, (2) preparative polyacrylamide...
Gespeichert in:
| Veröffentlicht in: | Journal of virological methods 1984-01, Vol.9 (4), p.283-291 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 291 |
|---|---|
| container_issue | 4 |
| container_start_page | 283 |
| container_title | Journal of virological methods |
| container_volume | 9 |
| creator | Vorndam, A.Vance Trent, Dennis W. |
| description | We have developed a procedure for purifying both the structural and nonstructural proteins of flaviviruses from lysates of infected cell cultures. The procedure involves: (l)immunoprecipitation to concentrate viral proteins and eliminate most of the cellular proteins, (2) preparative polyacrylamide gel electrophoresis to separate the viral proteins, and (3) hydroxyapatite chromatography, which eliminates most of the unlabeled cellular protein. This procedure offers an improvement over previous purification schemes in that there is no loss of viral proteins after the immunoprecipitation step, any combination of labeling isotopes may be used, and it is not necessary to soak proteins out of gel slices. |
| doi_str_mv | 10.1016/0166-0934(84)90054-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75908541</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0166093484900545</els_id><sourcerecordid>14095416</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-b0ccb70a962c784acd1b10fb1fdb10de343c826b580b28c826deae4c1a0c33fe3</originalsourceid><addsrcrecordid>eNqFkFFLHDEQgIO02Kv1H1jYhyLtw2pyyW6SF0FEbeGgBeuDTyE7mWBkb_eaZIX-e7PecfjUPoTJzHwzDB8hJ4yeMcra8_LammouvirxTVPaiLo5IAumpC5lJd6RxR75QD6m9EQLJDk_JIct1ZpLtiAPv6YYfACbwzhUo6_yI1YpxwnyFG1f2cFVwzi8qWzimDEMaYbv8lm1GqeQKhwAN4-2D7kkzyFO6RN5722f8HgXj8j9zfXvq-_16uftj6vLVQ2CyVx3FKCT1Op2CVIJC451jPqOeVeiQy44qGXbNYp2SzV_HVoUwCwFzj3yI3K63VsO-zNhymYdEmDf2wHHKRnZaKoawf4LMkF14doCii0IcUwpojebGNY2_jWMmlm9mb2a2atRwryqN00Z-7zbP3VrdPuhnevS_7Lr2wS299EOENIe00wyvZQFu9hiWKQ9B4wmQZj1uhARsnFj-PcdL9B3oRE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14095416</pqid></control><display><type>article</type><title>Purification of the structural and nonstructural proteins of St. Louis encephalitis virus</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Vorndam, A.Vance ; Trent, Dennis W.</creator><creatorcontrib>Vorndam, A.Vance ; Trent, Dennis W.</creatorcontrib><description>We have developed a procedure for purifying both the structural and nonstructural proteins of flaviviruses from lysates of infected cell cultures. The procedure involves: (l)immunoprecipitation to concentrate viral proteins and eliminate most of the cellular proteins, (2) preparative polyacrylamide gel electrophoresis to separate the viral proteins, and (3) hydroxyapatite chromatography, which eliminates most of the unlabeled cellular protein. This procedure offers an improvement over previous purification schemes in that there is no loss of viral proteins after the immunoprecipitation step, any combination of labeling isotopes may be used, and it is not necessary to soak proteins out of gel slices.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/0166-0934(84)90054-5</identifier><identifier>PMID: 6099371</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Biological and medical sciences ; Chromatography ; Electrophoresis, Polyacrylamide Gel ; Encephalitis Virus, St. Louis - analysis ; flavivirus ; Flavivirus - analysis ; Fundamental and applied biological sciences. Psychology ; Microbiology ; Morphology, structure, chemical composition, physicochemical properties ; protein purification ; proteins ; St Louis encephalitis virus ; Viral Proteins - analysis ; Viral Proteins - isolation & purification ; Virology</subject><ispartof>Journal of virological methods, 1984-01, Vol.9 (4), p.283-291</ispartof><rights>1984</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-b0ccb70a962c784acd1b10fb1fdb10de343c826b580b28c826deae4c1a0c33fe3</citedby><cites>FETCH-LOGICAL-c417t-b0ccb70a962c784acd1b10fb1fdb10de343c826b580b28c826deae4c1a0c33fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0166-0934(84)90054-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9171927$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6099371$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vorndam, A.Vance</creatorcontrib><creatorcontrib>Trent, Dennis W.</creatorcontrib><title>Purification of the structural and nonstructural proteins of St. Louis encephalitis virus</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>We have developed a procedure for purifying both the structural and nonstructural proteins of flaviviruses from lysates of infected cell cultures. The procedure involves: (l)immunoprecipitation to concentrate viral proteins and eliminate most of the cellular proteins, (2) preparative polyacrylamide gel electrophoresis to separate the viral proteins, and (3) hydroxyapatite chromatography, which eliminates most of the unlabeled cellular protein. This procedure offers an improvement over previous purification schemes in that there is no loss of viral proteins after the immunoprecipitation step, any combination of labeling isotopes may be used, and it is not necessary to soak proteins out of gel slices.</description><subject>Biological and medical sciences</subject><subject>Chromatography</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Encephalitis Virus, St. Louis - analysis</subject><subject>flavivirus</subject><subject>Flavivirus - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbiology</subject><subject>Morphology, structure, chemical composition, physicochemical properties</subject><subject>protein purification</subject><subject>proteins</subject><subject>St Louis encephalitis virus</subject><subject>Viral Proteins - analysis</subject><subject>Viral Proteins - isolation & purification</subject><subject>Virology</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkFFLHDEQgIO02Kv1H1jYhyLtw2pyyW6SF0FEbeGgBeuDTyE7mWBkb_eaZIX-e7PecfjUPoTJzHwzDB8hJ4yeMcra8_LammouvirxTVPaiLo5IAumpC5lJd6RxR75QD6m9EQLJDk_JIct1ZpLtiAPv6YYfACbwzhUo6_yI1YpxwnyFG1f2cFVwzi8qWzimDEMaYbv8lm1GqeQKhwAN4-2D7kkzyFO6RN5722f8HgXj8j9zfXvq-_16uftj6vLVQ2CyVx3FKCT1Op2CVIJC451jPqOeVeiQy44qGXbNYp2SzV_HVoUwCwFzj3yI3K63VsO-zNhymYdEmDf2wHHKRnZaKoawf4LMkF14doCii0IcUwpojebGNY2_jWMmlm9mb2a2atRwryqN00Z-7zbP3VrdPuhnevS_7Lr2wS299EOENIe00wyvZQFu9hiWKQ9B4wmQZj1uhARsnFj-PcdL9B3oRE</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>Vorndam, A.Vance</creator><creator>Trent, Dennis W.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19840101</creationdate><title>Purification of the structural and nonstructural proteins of St. Louis encephalitis virus</title><author>Vorndam, A.Vance ; Trent, Dennis W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-b0ccb70a962c784acd1b10fb1fdb10de343c826b580b28c826deae4c1a0c33fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Biological and medical sciences</topic><topic>Chromatography</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Encephalitis Virus, St. Louis - analysis</topic><topic>flavivirus</topic><topic>Flavivirus - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbiology</topic><topic>Morphology, structure, chemical composition, physicochemical properties</topic><topic>protein purification</topic><topic>proteins</topic><topic>St Louis encephalitis virus</topic><topic>Viral Proteins - analysis</topic><topic>Viral Proteins - isolation & purification</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vorndam, A.Vance</creatorcontrib><creatorcontrib>Trent, Dennis W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vorndam, A.Vance</au><au>Trent, Dennis W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification of the structural and nonstructural proteins of St. Louis encephalitis virus</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>1984-01-01</date><risdate>1984</risdate><volume>9</volume><issue>4</issue><spage>283</spage><epage>291</epage><pages>283-291</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>We have developed a procedure for purifying both the structural and nonstructural proteins of flaviviruses from lysates of infected cell cultures. The procedure involves: (l)immunoprecipitation to concentrate viral proteins and eliminate most of the cellular proteins, (2) preparative polyacrylamide gel electrophoresis to separate the viral proteins, and (3) hydroxyapatite chromatography, which eliminates most of the unlabeled cellular protein. This procedure offers an improvement over previous purification schemes in that there is no loss of viral proteins after the immunoprecipitation step, any combination of labeling isotopes may be used, and it is not necessary to soak proteins out of gel slices.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>6099371</pmid><doi>10.1016/0166-0934(84)90054-5</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0166-0934 |
| ispartof | Journal of virological methods, 1984-01, Vol.9 (4), p.283-291 |
| issn | 0166-0934 1879-0984 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75908541 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Biological and medical sciences Chromatography Electrophoresis, Polyacrylamide Gel Encephalitis Virus, St. Louis - analysis flavivirus Flavivirus - analysis Fundamental and applied biological sciences. Psychology Microbiology Morphology, structure, chemical composition, physicochemical properties protein purification proteins St Louis encephalitis virus Viral Proteins - analysis Viral Proteins - isolation & purification Virology |
| title | Purification of the structural and nonstructural proteins of St. Louis encephalitis virus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T11%3A17%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Purification%20of%20the%20structural%20and%20nonstructural%20proteins%20of%20St.%20Louis%20encephalitis%20virus&rft.jtitle=Journal%20of%20virological%20methods&rft.au=Vorndam,%20A.Vance&rft.date=1984-01-01&rft.volume=9&rft.issue=4&rft.spage=283&rft.epage=291&rft.pages=283-291&rft.issn=0166-0934&rft.eissn=1879-0984&rft.coden=JVMEDH&rft_id=info:doi/10.1016/0166-0934(84)90054-5&rft_dat=%3Cproquest_cross%3E14095416%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14095416&rft_id=info:pmid/6099371&rft_els_id=0166093484900545&rfr_iscdi=true |