Glutathione and bile acid synthesis. II. Effect of hepatic glutathione content on the activity and mRNA levels of cholesterol 7α-hydroxylase in the rat
Hepatic cholesterol 7α-hydroxylase (CH-7α) activity in intact rats depleted of glutathione (GSH) was reduced significantly (P < 0.007) compared with that in untreated controls. Northern blot analysis of poly A + mRNA isolated from GSH-depleted and control rat livers showed that there was a reduct...
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| Veröffentlicht in: | Biochemical pharmacology 1993-08, Vol.46 (3), p.555-556 |
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| container_title | Biochemical pharmacology |
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| creator | Hassan, Aslam S. Bunick, David St. Denis, Suzanne H. Lund, Lisa A. |
| description | Hepatic cholesterol 7α-hydroxylase (CH-7α) activity in intact rats depleted of glutathione (GSH) was reduced significantly (P < 0.007) compared with that in untreated controls. Northern blot analysis of poly A
+ mRNA isolated from GSH-depleted and control rat livers showed that there was a reduction in mRNA for CH-7α in treated rats that was commensurate with the reduction in CH-7α activity. The fact that the level of transferrin mRNA was unaltered by the depletion of GSH suggests that the change in
steady-state CH-7α mRNA content is
specifically sensitive to GSH content. This observation extends previous
in vitro findings and provides strong justification for a more detailed biochemical investigation into the interaction between GSH levels and the regulation of CH-7α mRNA levels. |
| doi_str_mv | 10.1016/0006-2952(93)90536-6 |
| format | Article |
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+ mRNA isolated from GSH-depleted and control rat livers showed that there was a reduction in mRNA for CH-7α in treated rats that was commensurate with the reduction in CH-7α activity. The fact that the level of transferrin mRNA was unaltered by the depletion of GSH suggests that the change in
steady-state CH-7α mRNA content is
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+ mRNA isolated from GSH-depleted and control rat livers showed that there was a reduction in mRNA for CH-7α in treated rats that was commensurate with the reduction in CH-7α activity. The fact that the level of transferrin mRNA was unaltered by the depletion of GSH suggests that the change in
steady-state CH-7α mRNA content is
specifically sensitive to GSH content. This observation extends previous
in vitro findings and provides strong justification for a more detailed biochemical investigation into the interaction between GSH levels and the regulation of CH-7α mRNA levels.</description><subject>Animals</subject><subject>Bile Acids and Salts - biosynthesis</subject><subject>Biological and medical sciences</subject><subject>Cholesterol 7-alpha-Hydroxylase - genetics</subject><subject>Cholesterol 7-alpha-Hydroxylase - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutathione - deficiency</subject><subject>Liver - metabolism</subject><subject>Liver. Bile. Biliary tracts</subject><subject>Male</subject><subject>Poly A - genetics</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - analysis</subject><subject>Transferrin - genetics</subject><subject>Vertebrates: digestive system</subject><issn>0006-2952</issn><issn>1873-2968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU2KFDEYDaKM7egNFLIQ0UW1SapSqWyEYRjHhkFBdB1SyVdWJF1pk3Rj3cRreBHPZGq6aVy5SvK9H768h9BzStaU0PYtIaStmOTstazfSMLrtmofoBXtRF3GbfcQrc6Ux-hJSt-XZ9fSC3TR1Y2goluhX7d-n3UeXZgA68ni3vlyMc7iNE95hOTSGm82a3wzDGAyDgMeYaezM_jbP1ITpgxTgSdcRMUgu4PL873l9vPHK-zhAD4tcjMGDylDDB6LP7-rcbYx_Jy9ToDdUR51fooeDdoneHY6L9HX9zdfrj9Ud59uN9dXd5WpCc0V8M5SwoSmxHLGB9lLwbpecMo1o0JL1kMNAJpB05OmY1YIbiizTS-t5Ka-RK-OvrsYfuzLXmrrkgHv9QRhn5TgJVohWSE2R6KJIaUIg9pFt9VxVpSopRC1xKuWtJWs1X0hqi2yFyf_fb8FexadGij4yxOuk9F-iHoyLp1ptWia8qFCe3eklRDh4CCqZBxMBqyLpRZlg_v_Hn8BmsOo9A</recordid><startdate>19930803</startdate><enddate>19930803</enddate><creator>Hassan, Aslam S.</creator><creator>Bunick, David</creator><creator>St. Denis, Suzanne H.</creator><creator>Lund, Lisa A.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930803</creationdate><title>Glutathione and bile acid synthesis. II. Effect of hepatic glutathione content on the activity and mRNA levels of cholesterol 7α-hydroxylase in the rat</title><author>Hassan, Aslam S. ; Bunick, David ; St. Denis, Suzanne H. ; Lund, Lisa A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-e58d1027a10d525f9b9728b7515a217a92be3eeea2e4b0482d775c12d4b9d95c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Bile Acids and Salts - biosynthesis</topic><topic>Biological and medical sciences</topic><topic>Cholesterol 7-alpha-Hydroxylase - genetics</topic><topic>Cholesterol 7-alpha-Hydroxylase - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutathione - deficiency</topic><topic>Liver - metabolism</topic><topic>Liver. Bile. Biliary tracts</topic><topic>Male</topic><topic>Poly A - genetics</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger - analysis</topic><topic>Transferrin - genetics</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hassan, Aslam S.</creatorcontrib><creatorcontrib>Bunick, David</creatorcontrib><creatorcontrib>St. Denis, Suzanne H.</creatorcontrib><creatorcontrib>Lund, Lisa A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hassan, Aslam S.</au><au>Bunick, David</au><au>St. Denis, Suzanne H.</au><au>Lund, Lisa A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glutathione and bile acid synthesis. II. Effect of hepatic glutathione content on the activity and mRNA levels of cholesterol 7α-hydroxylase in the rat</atitle><jtitle>Biochemical pharmacology</jtitle><addtitle>Biochem Pharmacol</addtitle><date>1993-08-03</date><risdate>1993</risdate><volume>46</volume><issue>3</issue><spage>555</spage><epage>556</epage><pages>555-556</pages><issn>0006-2952</issn><eissn>1873-2968</eissn><coden>BCPCA6</coden><abstract>Hepatic cholesterol 7α-hydroxylase (CH-7α) activity in intact rats depleted of glutathione (GSH) was reduced significantly (P < 0.007) compared with that in untreated controls. Northern blot analysis of poly A
+ mRNA isolated from GSH-depleted and control rat livers showed that there was a reduction in mRNA for CH-7α in treated rats that was commensurate with the reduction in CH-7α activity. The fact that the level of transferrin mRNA was unaltered by the depletion of GSH suggests that the change in
steady-state CH-7α mRNA content is
specifically sensitive to GSH content. This observation extends previous
in vitro findings and provides strong justification for a more detailed biochemical investigation into the interaction between GSH levels and the regulation of CH-7α mRNA levels.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>8347178</pmid><doi>10.1016/0006-2952(93)90536-6</doi><tpages>2</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-2952 |
| ispartof | Biochemical pharmacology, 1993-08, Vol.46 (3), p.555-556 |
| issn | 0006-2952 1873-2968 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Bile Acids and Salts - biosynthesis Biological and medical sciences Cholesterol 7-alpha-Hydroxylase - genetics Cholesterol 7-alpha-Hydroxylase - metabolism Fundamental and applied biological sciences. Psychology Glutathione - deficiency Liver - metabolism Liver. Bile. Biliary tracts Male Poly A - genetics Rats Rats, Sprague-Dawley RNA, Messenger - analysis Transferrin - genetics Vertebrates: digestive system |
| title | Glutathione and bile acid synthesis. II. Effect of hepatic glutathione content on the activity and mRNA levels of cholesterol 7α-hydroxylase in the rat |
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