Direct polymerase chain reaction detection of Campylobacter jejuni and Campylobacter coli in raw milk and dairy products
A polymerase chain reaction (PCR) method designed to sensitively detect and identify Campylobacter jejuni and Campylobacter coli without the need for isolating and culturing strains is described. The intergenic sequence between the flagellin genes flaA and flaB was amplified and characterized with a...
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| Veröffentlicht in: | Applied and Environmental Microbiology 1993-07, Vol.59 (7), p.2161-2165 |
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| creator | Wegmuller, B Luthy, J Candrian, U |
| description | A polymerase chain reaction (PCR) method designed to sensitively detect and identify Campylobacter jejuni and Campylobacter coli without the need for isolating and culturing strains is described. The intergenic sequence between the flagellin genes flaA and flaB was amplified and characterized with a triple primer or seminested primer approach. A total of 50 bacterial strains, 27 of C. jejuni and C. coli and 23 of other species, were tested, giving no false-positive or false-negative results. The detection limit as determined by ethidium bromide staining of amplification products on agarose gels was 10 bacteria or less in artificially contaminated water, milk, and soft cheese samples with the seminested primer PCR assay. As an application of the PCR system, a set of 93 samples of milk and other dairy products was screened for the presence of C. jejuni and C. coli. We identified six positive samples (6.5%), while none were found with a conventional culture method |
| doi_str_mv | 10.1128/aem.59.7.2161-2165.1993 |
| format | Article |
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The intergenic sequence between the flagellin genes flaA and flaB was amplified and characterized with a triple primer or seminested primer approach. A total of 50 bacterial strains, 27 of C. jejuni and C. coli and 23 of other species, were tested, giving no false-positive or false-negative results. The detection limit as determined by ethidium bromide staining of amplification products on agarose gels was 10 bacteria or less in artificially contaminated water, milk, and soft cheese samples with the seminested primer PCR assay. As an application of the PCR system, a set of 93 samples of milk and other dairy products was screened for the presence of C. jejuni and C. coli. We identified six positive samples (6.5%), while none were found with a conventional culture method</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.59.7.2161-2165.1993</identifier><identifier>PMID: 8357250</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Bacteriology ; Base Sequence ; BIOCHIMIE ; BIODEGRADACION ; BIODEGRADATION ; Biological and medical sciences ; BIOQUIMICA ; Biotechnology ; CAMPYLOBACTER ; Campylobacter coli ; Campylobacter coli - genetics ; Campylobacter coli - isolation & purification ; Campylobacter jejuni ; Campylobacter jejuni - genetics ; Campylobacter jejuni - isolation & purification ; CONTAMINACION ; CONTAMINATION ; Dairy products ; Dairy Products - microbiology ; DNA, Bacterial - genetics ; Food industries ; Food science ; FROMAGE ; FROMAGE A PATE MOLLE ; Fundamental and applied biological sciences. Psychology ; INFECCION ; INFECTION ; LAIT ; LECHE ; Milk - microbiology ; Milk and cheese industries. 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The intergenic sequence between the flagellin genes flaA and flaB was amplified and characterized with a triple primer or seminested primer approach. A total of 50 bacterial strains, 27 of C. jejuni and C. coli and 23 of other species, were tested, giving no false-positive or false-negative results. The detection limit as determined by ethidium bromide staining of amplification products on agarose gels was 10 bacteria or less in artificially contaminated water, milk, and soft cheese samples with the seminested primer PCR assay. As an application of the PCR system, a set of 93 samples of milk and other dairy products was screened for the presence of C. jejuni and C. coli. We identified six positive samples (6.5%), while none were found with a conventional culture method</description><subject>Animals</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>BIOCHIMIE</subject><subject>BIODEGRADACION</subject><subject>BIODEGRADATION</subject><subject>Biological and medical sciences</subject><subject>BIOQUIMICA</subject><subject>Biotechnology</subject><subject>CAMPYLOBACTER</subject><subject>Campylobacter coli</subject><subject>Campylobacter coli - genetics</subject><subject>Campylobacter coli - isolation & purification</subject><subject>Campylobacter jejuni</subject><subject>Campylobacter jejuni - genetics</subject><subject>Campylobacter jejuni - isolation & purification</subject><subject>CONTAMINACION</subject><subject>CONTAMINATION</subject><subject>Dairy products</subject><subject>Dairy Products - microbiology</subject><subject>DNA, Bacterial - genetics</subject><subject>Food industries</subject><subject>Food science</subject><subject>FROMAGE</subject><subject>FROMAGE A PATE MOLLE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>INFECCION</subject><subject>INFECTION</subject><subject>LAIT</subject><subject>LECHE</subject><subject>Milk - microbiology</subject><subject>Milk and cheese industries. Ice creams</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction - methods</subject><subject>QUESO</subject><subject>QUESO BLANDO</subject><subject>Sensitivity and Specificity</subject><subject>YAOURT</subject><subject>YOGUR</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkktv1DAUhS0EKtPCH0BChAqxS_A78YIFGp5SJRbQtXVrOzMekniwE8r8exwyGtFu2NiWz3euH_cg9ILgihDavAHXV0JVdUWJJGUeREWUYg_QimDVlIIx-RCtMFaqpJTjx-g8pR3GmGPZnKGzhomaCrxCv9_76MxY7EN36F2E5AqzBT8U0YEZfRgK60a3rEJbrKHfH7pwkzUXi53bTYMvYLD3BBM6X8xF4LbofffjL2LBx0Oxj8FOZkxP0KMWuuSeHucLdP3xw_f15_Lq66cv63dXpZGSjaWTlGBHDWADijhR48Yq0TKhQDDDW9UQa4VlxFAKYI0ByfOWZNJwzKllF-jtUnc_3fTOGjeMETq9j76HeNABvL6rDH6rN-GXJg2lgmT_66M_hp-TS6PufTKu62BwYUq6FgozXqv_grlFdU0xzeDlPXAXpjjkT9AUC0V5U4sM1QtkYkgpuvZ0Y4L1nACdE6CF0rWeEzAPQs8JyM7n_z745Du2POuvjjokA10bYTA-nTDeSIpxk7GXC7b1m-1tDomG1N89NDPPFqaFoGETc5nrb4pTyZVgfwCzks_W</recordid><startdate>19930701</startdate><enddate>19930701</enddate><creator>Wegmuller, B</creator><creator>Luthy, J</creator><creator>Candrian, U</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19930701</creationdate><title>Direct polymerase chain reaction detection of Campylobacter jejuni and Campylobacter coli in raw milk and dairy products</title><author>Wegmuller, B ; Luthy, J ; Candrian, U</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c663t-e6210e2ca0ca91e5708d95f359a53c4f981dd5d31c22aadcca6481d636c4042d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>BIOCHIMIE</topic><topic>BIODEGRADACION</topic><topic>BIODEGRADATION</topic><topic>Biological and medical sciences</topic><topic>BIOQUIMICA</topic><topic>Biotechnology</topic><topic>CAMPYLOBACTER</topic><topic>Campylobacter coli</topic><topic>Campylobacter coli - genetics</topic><topic>Campylobacter coli - isolation & purification</topic><topic>Campylobacter jejuni</topic><topic>Campylobacter jejuni - genetics</topic><topic>Campylobacter jejuni - isolation & purification</topic><topic>CONTAMINACION</topic><topic>CONTAMINATION</topic><topic>Dairy products</topic><topic>Dairy Products - microbiology</topic><topic>DNA, Bacterial - genetics</topic><topic>Food industries</topic><topic>Food science</topic><topic>FROMAGE</topic><topic>FROMAGE A PATE MOLLE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>INFECCION</topic><topic>INFECTION</topic><topic>LAIT</topic><topic>LECHE</topic><topic>Milk - microbiology</topic><topic>Milk and cheese industries. Ice creams</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction - methods</topic><topic>QUESO</topic><topic>QUESO BLANDO</topic><topic>Sensitivity and Specificity</topic><topic>YAOURT</topic><topic>YOGUR</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wegmuller, B</creatorcontrib><creatorcontrib>Luthy, J</creatorcontrib><creatorcontrib>Candrian, U</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wegmuller, B</au><au>Luthy, J</au><au>Candrian, U</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct polymerase chain reaction detection of Campylobacter jejuni and Campylobacter coli in raw milk and dairy products</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1993-07-01</date><risdate>1993</risdate><volume>59</volume><issue>7</issue><spage>2161</spage><epage>2165</epage><pages>2161-2165</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>A polymerase chain reaction (PCR) method designed to sensitively detect and identify Campylobacter jejuni and Campylobacter coli without the need for isolating and culturing strains is described. The intergenic sequence between the flagellin genes flaA and flaB was amplified and characterized with a triple primer or seminested primer approach. A total of 50 bacterial strains, 27 of C. jejuni and C. coli and 23 of other species, were tested, giving no false-positive or false-negative results. The detection limit as determined by ethidium bromide staining of amplification products on agarose gels was 10 bacteria or less in artificially contaminated water, milk, and soft cheese samples with the seminested primer PCR assay. As an application of the PCR system, a set of 93 samples of milk and other dairy products was screened for the presence of C. jejuni and C. coli. We identified six positive samples (6.5%), while none were found with a conventional culture method</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>8357250</pmid><doi>10.1128/aem.59.7.2161-2165.1993</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied and Environmental Microbiology, 1993-07, Vol.59 (7), p.2161-2165 |
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| source | American Society for Microbiology; MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Bacteriology Base Sequence BIOCHIMIE BIODEGRADACION BIODEGRADATION Biological and medical sciences BIOQUIMICA Biotechnology CAMPYLOBACTER Campylobacter coli Campylobacter coli - genetics Campylobacter coli - isolation & purification Campylobacter jejuni Campylobacter jejuni - genetics Campylobacter jejuni - isolation & purification CONTAMINACION CONTAMINATION Dairy products Dairy Products - microbiology DNA, Bacterial - genetics Food industries Food science FROMAGE FROMAGE A PATE MOLLE Fundamental and applied biological sciences. Psychology INFECCION INFECTION LAIT LECHE Milk - microbiology Milk and cheese industries. Ice creams Molecular Sequence Data Polymerase Chain Reaction - methods QUESO QUESO BLANDO Sensitivity and Specificity YAOURT YOGUR |
| title | Direct polymerase chain reaction detection of Campylobacter jejuni and Campylobacter coli in raw milk and dairy products |
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