Restoration of the antibody response to sheep erythrocytes in thymectomized Xenopus implanted with MHC-compatible or MHC-incompatible thymus

These experiments make use of an amphibian model system for investigating the role of the thymus in T helper cell education. Clawed toads ( Xenopus laevis ), thymectomized at 7 days, are unable to mount an antibody response to thymus-dependent antigens, such as sheep red blood cells (SRBC). When thy...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Development (Cambridge) 1984-12, Vol.84 (1), p.287-302
Hauptverfasser:	GEARING, A. J. H, CRIBBIN, F. A, HORTON, J. D
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibody Formation Biological and medical sciences Erythrocytes - immunology Fundamental and applied biological sciences. Psychology Fundamental immunology General aspects. Ontogeny. Phylogeny Hemolytic Plaque Technique Immunobiology Larva Leukocyte Count Major Histocompatibility Complex Ploidies Sheep T-Lymphocytes Thymectomy Thymus Gland - immunology Thymus Gland - transplantation Xenopus laevis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

container_end_page	302
container_issue	1
container_start_page	287
container_title	Development (Cambridge)
container_volume	84
creator	GEARING, A. J. H CRIBBIN, F. A HORTON, J. D
description	These experiments make use of an amphibian model system for investigating the role of the thymus in T helper cell education. Clawed toads ( Xenopus laevis ), thymectomized at 7 days, are unable to mount an antibody response to thymus-dependent antigens, such as sheep red blood cells (SRBC). When thymectomized larvae are implanted with larval thymuses (either irradiated or non-irradiated), incompatible at the major histocompatibility complex (MHC) or with MHC-compatible or -incompatible âadultâ thymuses, their splenic plaque-forming cell response and serum haemolytic antibody production to SRBC are both restored, to some extent. However, levels of mercaptoethanol-resistant antibody were extremely poor in those animals implanted with MHC-incompatible âadultâ thymus. Larval thymus implants were shown, by ploidy-labelling studies, to become repopulated with host-derived lymphocytes. Whether or not these lymphocytes acquire their MHC restriction specificities in the thymus awaits clarification.
doi_str_mv	10.1242/dev.84.1.287
format	Article
fullrecord	<record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75902911</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>75902911</sourcerecordid><originalsourceid>FETCH-LOGICAL-c382t-8f54c2f1815dd7ed832d8f5a2d6560c60007dcd4f664183dc2d74d4fb8f031c13</originalsourceid><addsrcrecordid>eNqFkc1u1DAUhS1EVaaFHVskLxCbksF_iZ1lNWopUhESAomdldg3jVESB9uhCs_Qh8bDjAo7VpbP_XTOtQ9CLynZUibYOws_t0ps6ZYp-QRtqJCyqCmrn6INqUtS0Lqmz9BZjN8JIbyS8hSdVrxWvBQb9PAZYvKhSc5P2Hc49YCbKbnW2xUHiLOfIuDkcewBZgxhTX3wZk0QsZsyvo5gkh_dL7D4G0x-XvJgnIdskpV7l3r88WZXGD_OOaQdAPvwR3HTP9reZ4nP0UnXDBFeHM9z9PX66svuprj99P7D7vK2MFyxVKiuFIZ1VNHSWglWcWaz1jBblRUxVX6mtMaKrqoEVdwaZqXI11Z1hFND-Tl6c_Cdg_-x5A_Qo4sGhrw0-CVqWdaE1fT_IBWcMSplBt8eQBN8jAE6PQc3NmHVlOh9Szq3pJXQVOeWMv7q6Lu0I9hH-FhLnr8-zptomqELzWRcfMRqKvfBGbs4YL276-9dAN06P_g7F1PcB8Lg57-hvwGuHa0b</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14322177</pqid></control><display><type>article</type><title>Restoration of the antibody response to sheep erythrocytes in thymectomized Xenopus implanted with MHC-compatible or MHC-incompatible thymus</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Company of Biologists</source><creator>GEARING, A. J. H ; CRIBBIN, F. A ; HORTON, J. D</creator><creatorcontrib>GEARING, A. J. H ; CRIBBIN, F. A ; HORTON, J. D</creatorcontrib><description>These experiments make use of an amphibian model system for investigating the role of the thymus in T helper cell education. Clawed toads ( Xenopus laevis ), thymectomized at 7 days, are unable to mount an antibody response to thymus-dependent antigens, such as sheep red blood cells (SRBC). When thymectomized larvae are implanted with larval thymuses (either irradiated or non-irradiated), incompatible at the major histocompatibility complex (MHC) or with MHC-compatible or -incompatible âadultâ thymuses, their splenic plaque-forming cell response and serum haemolytic antibody production to SRBC are both restored, to some extent. However, levels of mercaptoethanol-resistant antibody were extremely poor in those animals implanted with MHC-incompatible âadultâ thymus. Larval thymus implants were shown, by ploidy-labelling studies, to become repopulated with host-derived lymphocytes. Whether or not these lymphocytes acquire their MHC restriction specificities in the thymus awaits clarification.</description><identifier>ISSN: 0950-1991</identifier><identifier>ISSN: 0022-0752</identifier><identifier>EISSN: 1477-9129</identifier><identifier>DOI: 10.1242/dev.84.1.287</identifier><identifier>PMID: 6398354</identifier><language>eng</language><publisher>London: The Company of Biologists Limited</publisher><subject>Animals ; Antibody Formation ; Biological and medical sciences ; Erythrocytes - immunology ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; General aspects. Ontogeny. Phylogeny ; Hemolytic Plaque Technique ; Immunobiology ; Larva ; Leukocyte Count ; Major Histocompatibility Complex ; Ploidies ; Sheep ; T-Lymphocytes ; Thymectomy ; Thymus Gland - immunology ; Thymus Gland - transplantation ; Xenopus laevis</subject><ispartof>Development (Cambridge), 1984-12, Vol.84 (1), p.287-302</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-8f54c2f1815dd7ed832d8f5a2d6560c60007dcd4f664183dc2d74d4fb8f031c13</citedby><cites>FETCH-LOGICAL-c382t-8f54c2f1815dd7ed832d8f5a2d6560c60007dcd4f664183dc2d74d4fb8f031c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3665,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9174322$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6398354$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GEARING, A. J. H</creatorcontrib><creatorcontrib>CRIBBIN, F. A</creatorcontrib><creatorcontrib>HORTON, J. D</creatorcontrib><title>Restoration of the antibody response to sheep erythrocytes in thymectomized Xenopus implanted with MHC-compatible or MHC-incompatible thymus</title><title>Development (Cambridge)</title><addtitle>J Embryol Exp Morphol</addtitle><description>These experiments make use of an amphibian model system for investigating the role of the thymus in T helper cell education. Clawed toads ( Xenopus laevis ), thymectomized at 7 days, are unable to mount an antibody response to thymus-dependent antigens, such as sheep red blood cells (SRBC). When thymectomized larvae are implanted with larval thymuses (either irradiated or non-irradiated), incompatible at the major histocompatibility complex (MHC) or with MHC-compatible or -incompatible âadultâ thymuses, their splenic plaque-forming cell response and serum haemolytic antibody production to SRBC are both restored, to some extent. However, levels of mercaptoethanol-resistant antibody were extremely poor in those animals implanted with MHC-incompatible âadultâ thymus. Larval thymus implants were shown, by ploidy-labelling studies, to become repopulated with host-derived lymphocytes. Whether or not these lymphocytes acquire their MHC restriction specificities in the thymus awaits clarification.</description><subject>Animals</subject><subject>Antibody Formation</subject><subject>Biological and medical sciences</subject><subject>Erythrocytes - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>General aspects. Ontogeny. Phylogeny</subject><subject>Hemolytic Plaque Technique</subject><subject>Immunobiology</subject><subject>Larva</subject><subject>Leukocyte Count</subject><subject>Major Histocompatibility Complex</subject><subject>Ploidies</subject><subject>Sheep</subject><subject>T-Lymphocytes</subject><subject>Thymectomy</subject><subject>Thymus Gland - immunology</subject><subject>Thymus Gland - transplantation</subject><subject>Xenopus laevis</subject><issn>0950-1991</issn><issn>0022-0752</issn><issn>1477-9129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS1EVaaFHVskLxCbksF_iZ1lNWopUhESAomdldg3jVESB9uhCs_Qh8bDjAo7VpbP_XTOtQ9CLynZUibYOws_t0ps6ZYp-QRtqJCyqCmrn6INqUtS0Lqmz9BZjN8JIbyS8hSdVrxWvBQb9PAZYvKhSc5P2Hc49YCbKbnW2xUHiLOfIuDkcewBZgxhTX3wZk0QsZsyvo5gkh_dL7D4G0x-XvJgnIdskpV7l3r88WZXGD_OOaQdAPvwR3HTP9reZ4nP0UnXDBFeHM9z9PX66svuprj99P7D7vK2MFyxVKiuFIZ1VNHSWglWcWaz1jBblRUxVX6mtMaKrqoEVdwaZqXI11Z1hFND-Tl6c_Cdg_-x5A_Qo4sGhrw0-CVqWdaE1fT_IBWcMSplBt8eQBN8jAE6PQc3NmHVlOh9Szq3pJXQVOeWMv7q6Lu0I9hH-FhLnr8-zptomqELzWRcfMRqKvfBGbs4YL276-9dAN06P_g7F1PcB8Lg57-hvwGuHa0b</recordid><startdate>198412</startdate><enddate>198412</enddate><creator>GEARING, A. J. H</creator><creator>CRIBBIN, F. A</creator><creator>HORTON, J. D</creator><general>The Company of Biologists Limited</general><general>Cambridge University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>198412</creationdate><title>Restoration of the antibody response to sheep erythrocytes in thymectomized Xenopus implanted with MHC-compatible or MHC-incompatible thymus</title><author>GEARING, A. J. H ; CRIBBIN, F. A ; HORTON, J. D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-8f54c2f1815dd7ed832d8f5a2d6560c60007dcd4f664183dc2d74d4fb8f031c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Antibody Formation</topic><topic>Biological and medical sciences</topic><topic>Erythrocytes - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>General aspects. Ontogeny. Phylogeny</topic><topic>Hemolytic Plaque Technique</topic><topic>Immunobiology</topic><topic>Larva</topic><topic>Leukocyte Count</topic><topic>Major Histocompatibility Complex</topic><topic>Ploidies</topic><topic>Sheep</topic><topic>T-Lymphocytes</topic><topic>Thymectomy</topic><topic>Thymus Gland - immunology</topic><topic>Thymus Gland - transplantation</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GEARING, A. J. H</creatorcontrib><creatorcontrib>CRIBBIN, F. A</creatorcontrib><creatorcontrib>HORTON, J. D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Development (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GEARING, A. J. H</au><au>CRIBBIN, F. A</au><au>HORTON, J. D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Restoration of the antibody response to sheep erythrocytes in thymectomized Xenopus implanted with MHC-compatible or MHC-incompatible thymus</atitle><jtitle>Development (Cambridge)</jtitle><addtitle>J Embryol Exp Morphol</addtitle><date>1984-12</date><risdate>1984</risdate><volume>84</volume><issue>1</issue><spage>287</spage><epage>302</epage><pages>287-302</pages><issn>0950-1991</issn><issn>0022-0752</issn><eissn>1477-9129</eissn><abstract>These experiments make use of an amphibian model system for investigating the role of the thymus in T helper cell education. Clawed toads ( Xenopus laevis ), thymectomized at 7 days, are unable to mount an antibody response to thymus-dependent antigens, such as sheep red blood cells (SRBC). When thymectomized larvae are implanted with larval thymuses (either irradiated or non-irradiated), incompatible at the major histocompatibility complex (MHC) or with MHC-compatible or -incompatible âadultâ thymuses, their splenic plaque-forming cell response and serum haemolytic antibody production to SRBC are both restored, to some extent. However, levels of mercaptoethanol-resistant antibody were extremely poor in those animals implanted with MHC-incompatible âadultâ thymus. Larval thymus implants were shown, by ploidy-labelling studies, to become repopulated with host-derived lymphocytes. Whether or not these lymphocytes acquire their MHC restriction specificities in the thymus awaits clarification.</abstract><cop>London</cop><pub>The Company of Biologists Limited</pub><pmid>6398354</pmid><doi>10.1242/dev.84.1.287</doi><tpages>16</tpages></addata></record>
fulltext	fulltext
identifier	ISSN: 0950-1991
ispartof	Development (Cambridge), 1984-12, Vol.84 (1), p.287-302
issn	0950-1991 0022-0752 1477-9129
language	eng
recordid	cdi_proquest_miscellaneous_75902911
source	MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Company of Biologists
subjects	Animals Antibody Formation Biological and medical sciences Erythrocytes - immunology Fundamental and applied biological sciences. Psychology Fundamental immunology General aspects. Ontogeny. Phylogeny Hemolytic Plaque Technique Immunobiology Larva Leukocyte Count Major Histocompatibility Complex Ploidies Sheep T-Lymphocytes Thymectomy Thymus Gland - immunology Thymus Gland - transplantation Xenopus laevis
title	Restoration of the antibody response to sheep erythrocytes in thymectomized Xenopus implanted with MHC-compatible or MHC-incompatible thymus
url	https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T21%3A08%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Restoration%20of%20the%20antibody%20response%20to%20sheep%20erythrocytes%20in%20thymectomized%20Xenopus%20implanted%20with%20MHC-compatible%20or%20MHC-incompatible%20thymus&rft.jtitle=Development%20(Cambridge)&rft.au=GEARING,%20A.%20J.%20H&rft.date=1984-12&rft.volume=84&rft.issue=1&rft.spage=287&rft.epage=302&rft.pages=287-302&rft.issn=0950-1991&rft.eissn=1477-9129&rft_id=info:doi/10.1242/dev.84.1.287&rft_dat=%3Cproquest_cross%3E75902911%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14322177&rft_id=info:pmid/6398354&rfr_iscdi=true