IDENTIFICATION OF ENTEROTOXIGENIC ESCHERICHIA COLI-PRODUCING HEAT-LABILE ENTEROTOXIN BY AN ENZYME-LINKED IMMUNOSORBENT ASSAY ON SINGLE COLONIES GROWN ON MACCONKEY AGAR PLATES

An enzyme-linked immunosorbent assay (ELISA) using one antibody has been developed to detect heat-labile enterotoxin (LT) of Escherichia coli. The ELISA was able to detect as little as 0.1 ng/mL of purified LT. This ELISA was used successfully to identify LT-producing E. coli, using single colonies...

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Veröffentlicht in:	Journal of diarrhoeal diseases research 1984-12, Vol.2 (4), p.223-227
Hauptverfasser:	HONDA, TAKESHI, SATO, MINORU, KONGMUANG, URIRAT, MIWATANI, TOSHIO
Format:	Artikel
Sprache:	eng
Schlagworte:	Agar Antibodies Antigens Bacterial diseases Diarrhea Enterotoxins Enterotoxins - analysis Enterotoxins - immunology Enzyme linked immunosorbent assay Escherichia Escherichia coli Gangliosides Toxins
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container_title	Journal of diarrhoeal diseases research
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creator	HONDA, TAKESHI SATO, MINORU KONGMUANG, URIRAT MIWATANI, TOSHIO
description	An enzyme-linked immunosorbent assay (ELISA) using one antibody has been developed to detect heat-labile enterotoxin (LT) of Escherichia coli. The ELISA was able to detect as little as 0.1 ng/mL of purified LT. This ELISA was used successfully to identify LT-producing E. coli, using single colonies grown on a selective medium, MacConkey agar. The LT amount in one colony was calculated as 0.15 to 200 ng. As results can be available within 4-5 h after obtaining colonies, this ELISA allows very rapid diagnosis of diarrhoea caused by LT-producing E. coli.
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Aziz</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19841201</creationdate><title>IDENTIFICATION OF ENTEROTOXIGENIC ESCHERICHIA COLI-PRODUCING HEAT-LABILE ENTEROTOXIN BY AN ENZYME-LINKED IMMUNOSORBENT ASSAY ON SINGLE COLONIES GROWN ON MACCONKEY AGAR PLATES</title><author>HONDA, TAKESHI ; SATO, MINORU ; KONGMUANG, URIRAT ; MIWATANI, TOSHIO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j143t-dca2e68423810ab39aa37f23074a80abc8fd6c0bfea167be6017010d23cbc0423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Agar</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>Bacterial diseases</topic><topic>Diarrhea</topic><topic>Enterotoxins</topic><topic>Enterotoxins - analysis</topic><topic>Enterotoxins - immunology</topic><topic>Enzyme linked immunosorbent assay</topic><topic>Escherichia</topic><topic>Escherichia coli</topic><topic>Gangliosides</topic><topic>Toxins</topic><toplevel>online_resources</toplevel><creatorcontrib>HONDA, TAKESHI</creatorcontrib><creatorcontrib>SATO, MINORU</creatorcontrib><creatorcontrib>KONGMUANG, URIRAT</creatorcontrib><creatorcontrib>MIWATANI, TOSHIO</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of diarrhoeal diseases research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HONDA, TAKESHI</au><au>SATO, MINORU</au><au>KONGMUANG, URIRAT</au><au>MIWATANI, TOSHIO</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IDENTIFICATION OF ENTEROTOXIGENIC ESCHERICHIA COLI-PRODUCING HEAT-LABILE ENTEROTOXIN BY AN ENZYME-LINKED IMMUNOSORBENT ASSAY ON SINGLE COLONIES GROWN ON MACCONKEY AGAR PLATES</atitle><jtitle>Journal of diarrhoeal diseases research</jtitle><addtitle>J Diarrhoeal Dis Res</addtitle><date>1984-12-01</date><risdate>1984</risdate><volume>2</volume><issue>4</issue><spage>223</spage><epage>227</epage><pages>223-227</pages><issn>0253-8768</issn><eissn>2311-8512</eissn><abstract>An enzyme-linked immunosorbent assay (ELISA) using one antibody has been developed to detect heat-labile enterotoxin (LT) of Escherichia coli. 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subjects	Agar Antibodies Antigens Bacterial diseases Diarrhea Enterotoxins Enterotoxins - analysis Enterotoxins - immunology Enzyme linked immunosorbent assay Escherichia Escherichia coli Gangliosides Toxins
title	IDENTIFICATION OF ENTEROTOXIGENIC ESCHERICHIA COLI-PRODUCING HEAT-LABILE ENTEROTOXIN BY AN ENZYME-LINKED IMMUNOSORBENT ASSAY ON SINGLE COLONIES GROWN ON MACCONKEY AGAR PLATES
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