The purification of human creatine kinase BB with high specific activity

This paper describes the purification of human creatine kinase BB with high specific activity (1,122 U/mg). The procedure used resulted in a protein yield of 5.4 mg (21% recovery) from 150 g of brain tissue. Two-dimensional electrophoresis and PAGE studies indicated that purified CK-BB might exist a...

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Veröffentlicht in:	Clinica chimica acta 1984-12, Vol.144 (2), p.225-236
Hauptverfasser:	McBride, James H., Rodgerson, Denis O.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Brain - enzymology Chromatography, Gel Creatine Kinase - isolation & purification Creatine kinase BB Electrophoresis, Agar Gel Electrophoresis, Disc Enzyme purification Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology High specific activity Humans Isoelectric Focusing Isoenzymes Mercaptoethanol Molecular Weight Specimen Handling Staining and Labeling Transferases
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