A plasmid system for high-level expression and in vitro processing of recombinant proteins

A novel plasmid expression system has been constructed that combines two useful functions: it facilitates single-step affinity purification of cytoplasmically overproduced fusion proteins and the in vitro processing of fusions with IgA protease (Igase). The significant features directing the high ex...

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Veröffentlicht in:	Gene 1993-08, Vol.130 (1), p.121-126
Hauptverfasser:	Pohlner, Johannes, Krämer, Joachim, Meyer, Thomas F.
Format:	Artikel
Sprache:	eng
Schlagworte:	affinity purification Amino Acid Sequence Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - genetics Base Sequence Biological and medical sciences Biotechnology Carrier Proteins - metabolism Chromatography, Affinity Cloning, Molecular downstream box Escherichia coli - genetics Expression vector Fundamental and applied biological sciences. Psychology Gene Expression Genetic engineering Genetic technics Genetic Vectors Histidine IgA protease Immunoglobulin Heavy Chains - biosynthesis Immunoglobulin Heavy Chains - genetics Immunoglobulin Light Chains - biosynthesis Immunoglobulin Light Chains - genetics Methods. Procedures. Technologies Modification of gene expression level Molecular Sequence Data Nickel Oligonucleotides Peptide Hydrolases - metabolism Plasmids - genetics Protein Binding Protein Engineering - methods Protein Processing, Post-Translational recombinant antibody Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - isolation & purification Recombinant Fusion Proteins - metabolism sequence-specific cleavage Serine Endopeptidases
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